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The map label for this gene is nifD [H]

Identifier: 86748095

GI number: 86748095

Start: 1116194

End: 1117657

Strand: Direct

Name: nifD [H]

Synonym: RPB_0970

Alternate gene names: 86748095

Gene position: 1116194-1117657 (Clockwise)

Preceding gene: 86748094

Following gene: 86748096

Centisome position: 20.94

GC content: 61.2

Gene sequence:

>1464_bases
ATGAGCACCGCAGTCGCAGAATCCCCCGCGGACATCAAGGAACGCAACAAGCAGCTGATCGGCGAAGTCCTGGAAGCCTA
TCCGGACAAGTCCGCCAAGCGCCGCGCCAAGCACCTCAACACCTACGAGGCCGAAAAGGCCGAGTGTTCGGTCAAGTCCA
ACATCAAGTCGATCCCCGGCGTGATGACGATCCGCGGCTGCGCCTACGCCGGCTCCAAGGGCGTGGTGTGGGGTCCGATC
AAGGACATGGTCCACATCAGCCATGGTCCGGTCGGCTGCGGCCAGTATTCCTGGGGTTCGCGCCGCAACTACTACAAGGG
CAACACCGGCATCGACACCTTCGGCACGATGCAGTTCACCTCCGACTTCCAGGAGAAGGACATCGTCTTCGGCGGTGACA
AGAAGCTCGGCAAGATCATCGACGAAATTCAGGACCTGTTCCCGCTGAACCGCGGCATCTCGGTGCAGTCGGAATGCCCG
ATCGGCCTGATCGGCGACGACATCGAGGCGGTCTCCAAGGCCAAGACCAAGCAATACGACGGCAAGCCGATCATCCCGGT
GCGCTGCGAAGGCTTCCGCGGCGTGTCGCAGTCGCTCGGCCATCACATCGCCAACGACGTGATCCGCGACTGGGTGTTCG
ACAAGGCCGGCGACAAGGTCGCCACCTTCGAATCGACCCCCTACGACGTCGCGATCATCGGCGACTACAACATCGGCGGC
GACGCCTGGGCCTCGCGCATCCTGCTCGAGGAAATGGGTCTGCGCGTGATCGCGCAGTGGTCCGGCGACGGCACCATCGC
CGAGCTGGAGAACACCCCGAAGGCGAAGCTGAACATCCTGCATTGCTACCGCTCGATGAACTACATCACGCGGCACATGG
AAGAGAAGTTCGGGATCCCGTGGGTCGAGTACAATTTCTTCGGCCCGACCAAGATCGAAGCCTCGCTGCGAGAGATCGCC
GCGAAATTCGACGACAAGATCAAGGAAGGCGCCGAGCGCGTCATCGCCAAATACAAGCCGCGGATGCAGGCGATCGTCGA
TCGTTATCGCCCGCGCCTCGAAGGCAAGAAGGTCATGCTCTATGTCGGCGGCCTGCGTCCGCGGCACGTCATCGGCGCCT
ACGAAGACCTCGGCATGGAAGTGGTCGGCACCGGCTATGAATTCGGCCACAACGACGACTATCAGCGCACCACCCACTAC
GTGAAAGACGGCACGCTGATCTACGACGACGTCACCGGCTACGAATTCGAGAAGTTCGTCGAGAAGGTCCGGCCCGATCT
GGTCGGCTCCGGCATCAAGGAAAAGTACATCTTCCAGAAGATGGGTGTGCCGTTCCGCCAGATGCATTCGTGGGACTATT
CCGGCCCGTATCACGGCTATGACGGCTTCGCCATCTTCGCCCGCGACATGGACATCGCCATCAACGCTCCGATCTGGAAG
CTGACCAAGGCACCTTGGAGCTGA

Upstream 100 bases:

>100_bases
GGAAGCCGCCAAGGCGGCCGCCGCCACCGCCTGATCCATCAGCCGGGCCGGCCGCCTTGCGCGGCCGGCTCCCCGGCGGC
CCCTTACACGAGGAACAACG

Downstream 100 bases:

>100_bases
AGGTTCTGAGCTGAACCAACTCGTCACACAGGCTTCCGGTGCGATCCACCGGAAGCCCCGTGTCCCCGAATTCCTTGGGA
AAGAACATCAACATGACCGA

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSTAVAESPADIKERNKQLIGEVLEAYPDKSAKRRAKHLNTYEAEKAECSVKSNIKSIPGVMTIRGCAYAGSKGVVWGPI
KDMVHISHGPVGCGQYSWGSRRNYYKGNTGIDTFGTMQFTSDFQEKDIVFGGDKKLGKIIDEIQDLFPLNRGISVQSECP
IGLIGDDIEAVSKAKTKQYDGKPIIPVRCEGFRGVSQSLGHHIANDVIRDWVFDKAGDKVATFESTPYDVAIIGDYNIGG
DAWASRILLEEMGLRVIAQWSGDGTIAELENTPKAKLNILHCYRSMNYITRHMEEKFGIPWVEYNFFGPTKIEASLREIA
AKFDDKIKEGAERVIAKYKPRMQAIVDRYRPRLEGKKVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHY
VKDGTLIYDDVTGYEFEKFVEKVRPDLVGSGIKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDIAINAPIWK
LTKAPWS

Sequences:

>Translated_487_residues
MSTAVAESPADIKERNKQLIGEVLEAYPDKSAKRRAKHLNTYEAEKAECSVKSNIKSIPGVMTIRGCAYAGSKGVVWGPI
KDMVHISHGPVGCGQYSWGSRRNYYKGNTGIDTFGTMQFTSDFQEKDIVFGGDKKLGKIIDEIQDLFPLNRGISVQSECP
IGLIGDDIEAVSKAKTKQYDGKPIIPVRCEGFRGVSQSLGHHIANDVIRDWVFDKAGDKVATFESTPYDVAIIGDYNIGG
DAWASRILLEEMGLRVIAQWSGDGTIAELENTPKAKLNILHCYRSMNYITRHMEEKFGIPWVEYNFFGPTKIEASLREIA
AKFDDKIKEGAERVIAKYKPRMQAIVDRYRPRLEGKKVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHY
VKDGTLIYDDVTGYEFEKFVEKVRPDLVGSGIKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDIAINAPIWK
LTKAPWS
>Mature_486_residues
STAVAESPADIKERNKQLIGEVLEAYPDKSAKRRAKHLNTYEAEKAECSVKSNIKSIPGVMTIRGCAYAGSKGVVWGPIK
DMVHISHGPVGCGQYSWGSRRNYYKGNTGIDTFGTMQFTSDFQEKDIVFGGDKKLGKIIDEIQDLFPLNRGISVQSECPI
GLIGDDIEAVSKAKTKQYDGKPIIPVRCEGFRGVSQSLGHHIANDVIRDWVFDKAGDKVATFESTPYDVAIIGDYNIGGD
AWASRILLEEMGLRVIAQWSGDGTIAELENTPKAKLNILHCYRSMNYITRHMEEKFGIPWVEYNFFGPTKIEASLREIAA
KFDDKIKEGAERVIAKYKPRMQAIVDRYRPRLEGKKVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHYV
KDGTLIYDDVTGYEFEKFVEKVRPDLVGSGIKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDIAINAPIWKL
TKAPWS

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54830; Mature: 54699

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAVAESPADIKERNKQLIGEVLEAYPDKSAKRRAKHLNTYEAEKAECSVKSNIKSIPG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMVHISHGPVGCGQYSWGSRRNYYKGNTGIDTFGTMQFT
EEEEEEEEECCCCCCEEECHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
SDFQEKDIVFGGDKKLGKIIDEIQDLFPLNRGISVQSECPIGLIGDDIEAVSKAKTKQYD
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCC
GKPIIPVRCEGFRGVSQSLGHHIANDVIRDWVFDKAGDKVATFESTPYDVAIIGDYNIGG
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCH
DAWASRILLEEMGLRVIAQWSGDGTIAELENTPKAKLNILHCYRSMNYITRHMEEKFGIP
HHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
WVEYNFFGPTKIEASLREIAAKFDDKIKEGAERVIAKYKPRMQAIVDRYRPRLEGKKVML
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEE
YVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHYVKDGTLIYDDVTGYEFEKFV
EECCCCCHHHCCCHHHCCCEEEECCEECCCCCCHHHHHHEEECCEEEEECCCCCHHHHHH
EKVRPDLVGSGIKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDIAINAPIWK
HHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCEEECCCCEE
LTKAPWS
CCCCCCC
>Mature Secondary Structure 
STAVAESPADIKERNKQLIGEVLEAYPDKSAKRRAKHLNTYEAEKAECSVKSNIKSIPG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMVHISHGPVGCGQYSWGSRRNYYKGNTGIDTFGTMQFT
EEEEEEEEECCCCCCEEECHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
SDFQEKDIVFGGDKKLGKIIDEIQDLFPLNRGISVQSECPIGLIGDDIEAVSKAKTKQYD
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCC
GKPIIPVRCEGFRGVSQSLGHHIANDVIRDWVFDKAGDKVATFESTPYDVAIIGDYNIGG
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCH
DAWASRILLEEMGLRVIAQWSGDGTIAELENTPKAKLNILHCYRSMNYITRHMEEKFGIP
HHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
WVEYNFFGPTKIEASLREIAAKFDDKIKEGAERVIAKYKPRMQAIVDRYRPRLEGKKVML
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEE
YVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHYVKDGTLIYDDVTGYEFEKFV
EECCCCCHHHCCCHHHCCCEEEECCEECCCCCCHHHHHHEEECCEEEEECCCCCHHHHHH
EKVRPDLVGSGIKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDIAINAPIWK
HHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCEEECCCCEE
LTKAPWS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275; 6588133 [H]