Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 86748066

GI number: 86748066

Start: 1088626

End: 1089483

Strand: Direct

Name: braD [H]

Synonym: RPB_0941

Alternate gene names: 86748066

Gene position: 1088626-1089483 (Clockwise)

Preceding gene: 86748065

Following gene: 86748067

Centisome position: 20.42

GC content: 64.57

Gene sequence:

>858_bases
ATGGAAAATCTGCTCGTCGCTTTGTTCGAAATCCTGAGCTTCGGCGCCATCGTCGTGTTGGTGGTGCTGGGGCTCGGGAT
CATCGCCAGCATGATGGGGATCTTCAATTTCGCGCAGGGCGAATTCGTCCTGCTCGGCGCTTACGTCACTTATCTCGCTT
ACACGGCCGGGCTGCCGGTGTGGCTCGGCATGGTGGCGGCGCCGTTCGTCGTCGGCGCGCTGGGCTTCGTGCTCGAACGT
CTGATCGTTCGCAGATTCTACGCCGCGCCGATCGTGGCGATGCTCGGGACCTATGCGCTCGGCCTGATCATTCGCGAAAT
CGTCCGTGGCCTGATCGGCGGTCTCTATATCTCCGTGCCGGAGCCGGTCGGCGGTTCAGTTGCTCTGGGCGCGATGCATT
TCTCGACCTGGCGGCTGGTGATCATCGTCATCACCGCGCTGGTGATGGTGGCGAGTTATCTGTTGCTGTCGCGCACCTCG
TTCGGCCTGCGGGTCCGTGCCTCATTGGAGAATCCAGCGCTCGCGCGCGCCTCGGGGATCTCCACCAACGCGATCTATGG
CGCAACCTTTGCGTTCGGCGCGGCGCTGGCGGGCCTCGCCGGTGCGCTGATCGTACCGGTGTTCTCGCTGTTCGCCGATC
TCGGCCTGCGCTTCCTGATCCAGGGCTTCGTCGCCGTGATGGTCGGCGGCATCGGATCGTTCGCAGGCCCGGTCGCCGGC
GCGGCGGTGATCGGCACGCTGTCCGCGGCGCTGCCCTGGGTGATCCAGCCGGTCGTCGCCGACGTGCTCGTCTTCGTCCT
CGCCATCATCTTCATCAAGTTCCGGCCGCAGGGCCTCGTTTCGGGAAAAGGGATCTAG

Upstream 100 bases:

>100_bases
ACTGGAAGACGATGTGAGCGCCGTGATCCGGACAAGCGTCGACCGGTTGTCCGGATCGCCCCCACGTCGCAACGCTTGCC
ACCCCATCCGGTAAGAACGA

Downstream 100 bases:

>100_bases
CCTGATGTCCAGCGACAACAAACACGCGTTCACCCGCCGCCGTTTCCTCTCCAACTTCGCCTTCGCCGGCACCGCGATCG
CCACCGGCGTCGGCAGCTGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MENLLVALFEILSFGAIVVLVVLGLGIIASMMGIFNFAQGEFVLLGAYVTYLAYTAGLPVWLGMVAAPFVVGALGFVLER
LIVRRFYAAPIVAMLGTYALGLIIREIVRGLIGGLYISVPEPVGGSVALGAMHFSTWRLVIIVITALVMVASYLLLSRTS
FGLRVRASLENPALARASGISTNAIYGATFAFGAALAGLAGALIVPVFSLFADLGLRFLIQGFVAVMVGGIGSFAGPVAG
AAVIGTLSAALPWVIQPVVADVLVFVLAIIFIKFRPQGLVSGKGI

Sequences:

>Translated_285_residues
MENLLVALFEILSFGAIVVLVVLGLGIIASMMGIFNFAQGEFVLLGAYVTYLAYTAGLPVWLGMVAAPFVVGALGFVLER
LIVRRFYAAPIVAMLGTYALGLIIREIVRGLIGGLYISVPEPVGGSVALGAMHFSTWRLVIIVITALVMVASYLLLSRTS
FGLRVRASLENPALARASGISTNAIYGATFAFGAALAGLAGALIVPVFSLFADLGLRFLIQGFVAVMVGGIGSFAGPVAG
AAVIGTLSAALPWVIQPVVADVLVFVLAIIFIKFRPQGLVSGKGI
>Mature_285_residues
MENLLVALFEILSFGAIVVLVVLGLGIIASMMGIFNFAQGEFVLLGAYVTYLAYTAGLPVWLGMVAAPFVVGALGFVLER
LIVRRFYAAPIVAMLGTYALGLIIREIVRGLIGGLYISVPEPVGGSVALGAMHFSTWRLVIIVITALVMVASYLLLSRTS
FGLRVRASLENPALARASGISTNAIYGATFAFGAALAGLAGALIVPVFSLFADLGLRFLIQGFVAVMVGGIGSFAGPVAG
AAVIGTLSAALPWVIQPVVADVLVFVLAIIFIKFRPQGLVSGKGI

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=292, Percent_Identity=26.3698630136986, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 29620; Mature: 29620

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLLVALFEILSFGAIVVLVVLGLGIIASMMGIFNFAQGEFVLLGAYVTYLAYTAGLPV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCHHH
WLGMVAAPFVVGALGFVLERLIVRRFYAAPIVAMLGTYALGLIIREIVRGLIGGLYISVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
EPVGGSVALGAMHFSTWRLVIIVITALVMVASYLLLSRTSFGLRVRASLENPALARASGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCC
STNAIYGATFAFGAALAGLAGALIVPVFSLFADLGLRFLIQGFVAVMVGGIGSFAGPVAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVIGTLSAALPWVIQPVVADVLVFVLAIIFIKFRPQGLVSGKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MENLLVALFEILSFGAIVVLVVLGLGIIASMMGIFNFAQGEFVLLGAYVTYLAYTAGLPV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCHHH
WLGMVAAPFVVGALGFVLERLIVRRFYAAPIVAMLGTYALGLIIREIVRGLIGGLYISVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
EPVGGSVALGAMHFSTWRLVIIVITALVMVASYLLLSRTSFGLRVRASLENPALARASGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCC
STNAIYGATFAFGAALAGLAGALIVPVFSLFADLGLRFLIQGFVAVMVGGIGSFAGPVAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVIGTLSAALPWVIQPVVADVLVFVLAIIFIKFRPQGLVSGKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]