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The map label for this gene is 86748065

Identifier: 86748065

GI number: 86748065

Start: 1087028

End: 1088542

Strand: Direct

Name: 86748065

Synonym: RPB_0940

Alternate gene names: NA

Gene position: 1087028-1088542 (Clockwise)

Preceding gene: 86748064

Following gene: 86748066

Centisome position: 20.39

GC content: 65.74

Gene sequence:

>1515_bases
TTGGCCGTTTCACTTCCCACCCCCACGCAGCTTCTCGACGTCGCCGATCAGTGCGGCCTGTCGCTCACCGATGAGGACGT
GATGTCCTTCCGCGCGCTGATGCAGCCCTCGGTCGATGCCTACAATGTCGTCGCCGCGTTGCCGGACGAAGTGCCGCTGG
TGAAGTATCCGCGCACGCCCGGCTACTCGCCGGACTCCGACGAGAATCCGCGCAACGCCTGGTACCGCAAGTCGAGCGTG
AAGGGCGCCGCGTCGGGCAAGCTGACAGGCAAGACCATCGCGCTCAAGGACAACATCATGCTGGCCGGCGTGCCGATGAT
GAACGGCTCCTCGACGCTCGAAGGCTTCGTCCCGGATTTCGACGCGACCGTCGTGATGCGGATTCTCGATGCCGGCGGCG
AAATTCTCGGCAAGACGCATTGCGAACATTTCTGCATGTCCGGCGGCAGCCACACCGGCTCCAACGGTCCGGTGCACAAT
CCGCACAAGATGGGCTATTCGGCCGGCGGTTCGTCGTCGGGCAGTGCCGTCGTGGTCGCGCTCGGCGAGGCCGACATGGC
GATCGGCGGCGACCAGGGCGGCTCGATCCGGATGCCGGCGTCGTTCAGCGGCATCTACGGCATGAAGCCGACATGGGGCC
TGGTGCCCTACACCGGCATCATGCCGATCGAAATCTTCGTCGATCACACCGGCCCGATGACCGCGAGCGTCGCGGACAAT
GCGCTGTTGCTGGAAGTGCTCGCGGGCGACGACGGCTACGATCCACGGATCCGCGCACCGGTCGTCCACGACTACACCAA
AGCGCTCGGCGGCGGCGTCAAGGGCATGAAGATCGGCATCGTCCGCGAAGGCTTCGAACAGGCCGGCGCCGAGCCGGCGG
TCAACGAGAGCGTGCGCGAGGCCGCCAAGCGGCTCGCGAGCCTCGGCGCCACCGTCGAGGAGATCTCGATCCCAGCGCAC
AATCTCGCCGCCGCGGTGTGGATGCCGATCGGAACCGAGGGCATGACGCAGACCATGATGTTCGGTGACGGTTACGGCCT
CAGCCGTCCCGATCTGTATTCCACCGCGCTCATGAAGGCGCATCGCGGCTGGCGCGAACAGGCGGATTCGCTGTCCGAAA
CCACCAAGATGATGCTGCTGTTCGGCACCTACATCAACAACACATTCGGCCCGCGCTATTACGGCAAGGCGCAGAACATC
TCGCGCCGGGTCACCGCCGCCTATGATGCGGCGCTGGCGAAGTTCGATCTGCTGCTGATGCCGACGACGCCGATGAAGGC
GACGCCGCTGCCGCCGCCCGGCGCCAGCCGCGAGGAGGTCATCGGCCGCGCCTTCGAGATGATCAGCAACACCGCGCCGT
TCGACATCACCCATCACCCGGCGATGTCGCTGCCCTGCGGCATGGTCGACGGCCTGCCGGTCGGGCTGATGTTGATCGGC
AAGCATTTCGACGAGCCGACGATCTATCGCGCGGCGCACGCCTTCGAGCAGTCCGGCGACTGGAAGACGATGTGA

Upstream 100 bases:

>100_bases
ATATTTCCGTATGTAGTATCCATGACGCGGCGGCCCGCGAGCCGCAGCCGGTCTTCCCCGGACCGGCGCCGCCGGTCTGC
TCATGCGCAGGAGGACAACC

Downstream 100 bases:

>100_bases
GCGCCGTGATCCGGACAAGCGTCGACCGGTTGTCCGGATCGCCCCCACGTCGCAACGCTTGCCACCCCATCCGGTAAGAA
CGAATGGAAAATCTGCTCGT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAVSLPTPTQLLDVADQCGLSLTDEDVMSFRALMQPSVDAYNVVAALPDEVPLVKYPRTPGYSPDSDENPRNAWYRKSSV
KGAASGKLTGKTIALKDNIMLAGVPMMNGSSTLEGFVPDFDATVVMRILDAGGEILGKTHCEHFCMSGGSHTGSNGPVHN
PHKMGYSAGGSSSGSAVVVALGEADMAIGGDQGGSIRMPASFSGIYGMKPTWGLVPYTGIMPIEIFVDHTGPMTASVADN
ALLLEVLAGDDGYDPRIRAPVVHDYTKALGGGVKGMKIGIVREGFEQAGAEPAVNESVREAAKRLASLGATVEEISIPAH
NLAAAVWMPIGTEGMTQTMMFGDGYGLSRPDLYSTALMKAHRGWREQADSLSETTKMMLLFGTYINNTFGPRYYGKAQNI
SRRVTAAYDAALAKFDLLLMPTTPMKATPLPPPGASREEVIGRAFEMISNTAPFDITHHPAMSLPCGMVDGLPVGLMLIG
KHFDEPTIYRAAHAFEQSGDWKTM

Sequences:

>Translated_504_residues
MAVSLPTPTQLLDVADQCGLSLTDEDVMSFRALMQPSVDAYNVVAALPDEVPLVKYPRTPGYSPDSDENPRNAWYRKSSV
KGAASGKLTGKTIALKDNIMLAGVPMMNGSSTLEGFVPDFDATVVMRILDAGGEILGKTHCEHFCMSGGSHTGSNGPVHN
PHKMGYSAGGSSSGSAVVVALGEADMAIGGDQGGSIRMPASFSGIYGMKPTWGLVPYTGIMPIEIFVDHTGPMTASVADN
ALLLEVLAGDDGYDPRIRAPVVHDYTKALGGGVKGMKIGIVREGFEQAGAEPAVNESVREAAKRLASLGATVEEISIPAH
NLAAAVWMPIGTEGMTQTMMFGDGYGLSRPDLYSTALMKAHRGWREQADSLSETTKMMLLFGTYINNTFGPRYYGKAQNI
SRRVTAAYDAALAKFDLLLMPTTPMKATPLPPPGASREEVIGRAFEMISNTAPFDITHHPAMSLPCGMVDGLPVGLMLIG
KHFDEPTIYRAAHAFEQSGDWKTM
>Mature_503_residues
AVSLPTPTQLLDVADQCGLSLTDEDVMSFRALMQPSVDAYNVVAALPDEVPLVKYPRTPGYSPDSDENPRNAWYRKSSVK
GAASGKLTGKTIALKDNIMLAGVPMMNGSSTLEGFVPDFDATVVMRILDAGGEILGKTHCEHFCMSGGSHTGSNGPVHNP
HKMGYSAGGSSSGSAVVVALGEADMAIGGDQGGSIRMPASFSGIYGMKPTWGLVPYTGIMPIEIFVDHTGPMTASVADNA
LLLEVLAGDDGYDPRIRAPVVHDYTKALGGGVKGMKIGIVREGFEQAGAEPAVNESVREAAKRLASLGATVEEISIPAHN
LAAAVWMPIGTEGMTQTMMFGDGYGLSRPDLYSTALMKAHRGWREQADSLSETTKMMLLFGTYINNTFGPRYYGKAQNIS
RRVTAAYDAALAKFDLLLMPTTPMKATPLPPPGASREEVIGRAFEMISNTAPFDITHHPAMSLPCGMVDGLPVGLMLIGK
HFDEPTIYRAAHAFEQSGDWKTM

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=455, Percent_Identity=29.010989010989, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI195972892, Length=437, Percent_Identity=25.4004576659039, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=430, Percent_Identity=27.906976744186, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537465, Length=428, Percent_Identity=23.3644859813084, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=242, Percent_Identity=26.8595041322314, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538252, Length=232, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=440, Percent_Identity=27.2727272727273, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6319685, Length=441, Percent_Identity=24.9433106575964, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648113, Length=441, Percent_Identity=30.3854875283447, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI161078093, Length=430, Percent_Identity=25.1162790697674, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644968, Length=450, Percent_Identity=24.8888888888889, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648435, Length=170, Percent_Identity=31.7647058823529, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648437, Length=170, Percent_Identity=31.7647058823529, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45550774, Length=170, Percent_Identity=31.7647058823529, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648441, Length=179, Percent_Identity=30.7262569832402, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=179, Percent_Identity=30.7262569832402, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652985, Length=209, Percent_Identity=28.7081339712919, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922090, Length=209, Percent_Identity=28.7081339712919, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652981, Length=209, Percent_Identity=28.7081339712919, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652983, Length=209, Percent_Identity=28.7081339712919, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 53614; Mature: 53483

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSLPTPTQLLDVADQCGLSLTDEDVMSFRALMQPSVDAYNVVAALPDEVPLVKYPRTP
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHEEECCCCCCEEECCCCC
GYSPDSDENPRNAWYRKSSVKGAASGKLTGKTIALKDNIMLAGVPMMNGSSTLEGFVPDF
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
DATVVMRILDAGGEILGKTHCEHFCMSGGSHTGSNGPVHNPHKMGYSAGGSSSGSAVVVA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEE
LGEADMAIGGDQGGSIRMPASFSGIYGMKPTWGLVPYTGIMPIEIFVDHTGPMTASVADN
ECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHCCC
ALLLEVLAGDDGYDPRIRAPVVHDYTKALGGGVKGMKIGIVREGFEQAGAEPAVNESVRE
EEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHH
AAKRLASLGATVEEISIPAHNLAAAVWMPIGTEGMTQTMMFGDGYGLSRPDLYSTALMKA
HHHHHHHHCCCHHHHCCCHHHHEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
HRGWREQADSLSETTKMMLLFGTYINNTFGPRYYGKAQNISRRVTAAYDAALAKFDLLLM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
PTTPMKATPLPPPGASREEVIGRAFEMISNTAPFDITHHPAMSLPCGMVDGLPVGLMLIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
KHFDEPTIYRAAHAFEQSGDWKTM
CCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AVSLPTPTQLLDVADQCGLSLTDEDVMSFRALMQPSVDAYNVVAALPDEVPLVKYPRTP
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHEEECCCCCCEEECCCCC
GYSPDSDENPRNAWYRKSSVKGAASGKLTGKTIALKDNIMLAGVPMMNGSSTLEGFVPDF
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
DATVVMRILDAGGEILGKTHCEHFCMSGGSHTGSNGPVHNPHKMGYSAGGSSSGSAVVVA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEE
LGEADMAIGGDQGGSIRMPASFSGIYGMKPTWGLVPYTGIMPIEIFVDHTGPMTASVADN
ECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHCCC
ALLLEVLAGDDGYDPRIRAPVVHDYTKALGGGVKGMKIGIVREGFEQAGAEPAVNESVRE
EEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHH
AAKRLASLGATVEEISIPAHNLAAAVWMPIGTEGMTQTMMFGDGYGLSRPDLYSTALMKA
HHHHHHHHCCCHHHHCCCHHHHEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
HRGWREQADSLSETTKMMLLFGTYINNTFGPRYYGKAQNISRRVTAAYDAALAKFDLLLM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
PTTPMKATPLPPPGASREEVIGRAFEMISNTAPFDITHHPAMSLPCGMVDGLPVGLMLIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
KHFDEPTIYRAAHAFEQSGDWKTM
CCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]