Click here to switch to the map view.

The map label for this gene is maiA [H]

Identifier: 86748034

GI number: 86748034

Start: 1045512

End: 1046168

Strand: Direct

Name: maiA [H]

Synonym: RPB_0908

Alternate gene names: 86748034

Gene position: 1045512-1046168 (Clockwise)

Preceding gene: 86748033

Following gene: 86748035

Centisome position: 19.61

GC content: 64.99

Gene sequence:

>657_bases
ATGACCAAGACCGCTGATCACGACGCCGCGGTGCTGTACGGTTACTTCCGGTCCTCCGCGGCCTATCGGGTGCGCATCGC
GCTCAATCTGAAGGGCGTCGTCGTCGCGCAGCGCTACGTGCATCTGCGCGACGGCGAGCAGAATCTCGAAGCCTACCGCT
GGATCAATCCCGCCGGATCGGTGCCGTTTTGGCGCGAGGGCGATTTCGATCTCGCGCAATCGCTGGCGATCATCGAATAT
CTCGACGAGACCCATCCCGAGCCGCCGCTGCTGCCGAGAGATCCCAGGGCCCGTGCGATCGTCCGCGAAATGGCCTACGC
GGTCGCCTGCGACATCCATCCGCTCGGCAATCTGCGGATTCTGAAACGGCTGACCGAGCTCGGCATCGACGAGATCGAGC
GCGCGCGCTGGTCGAAGCAATGGATCGAGCAGGGTTTTGTGGCGATCGAGGCGCGGCTTTCGCAGACGCCGGGGCCGTTT
GCTTATGGAGATCAGCCGACGCTGGCCGATCTGTGCATCGTGCCGCAGGTCTTCAACGCCCGTCGCTTCGACGCCGACCT
CGCCCCGTTCGAACGCATCCGCCAGATCGAAGCCGAAGCGATGAAACTCGACGCCTTCGTCGCCGCCGAGCCGGGCCGGC
AGCCGGATGCGGAGTGA

Upstream 100 bases:

>100_bases
CGCAGCGCGTCACCGAATATGCCGCGACCGCCGGCACGCTGCAGGACGACTACGCCGATTGCTGGCGCGGCCTGGAGAAG
CGCTTCGACCCGAGCCGGCC

Downstream 100 bases:

>100_bases
TGTTGGACAACCACACCTCGCGCCTGCGCACCCGCTAACCTCTCCCCGCGCGCGGGGAGAGGTCGACCGGCGTAGCGCAG
CGAAGCCGGGCGGGTGAGGG

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTKTADHDAAVLYGYFRSSAAYRVRIALNLKGVVVAQRYVHLRDGEQNLEAYRWINPAGSVPFWREGDFDLAQSLAIIEY
LDETHPEPPLLPRDPRARAIVREMAYAVACDIHPLGNLRILKRLTELGIDEIERARWSKQWIEQGFVAIEARLSQTPGPF
AYGDQPTLADLCIVPQVFNARRFDADLAPFERIRQIEAEAMKLDAFVAAEPGRQPDAE

Sequences:

>Translated_218_residues
MTKTADHDAAVLYGYFRSSAAYRVRIALNLKGVVVAQRYVHLRDGEQNLEAYRWINPAGSVPFWREGDFDLAQSLAIIEY
LDETHPEPPLLPRDPRARAIVREMAYAVACDIHPLGNLRILKRLTELGIDEIERARWSKQWIEQGFVAIEARLSQTPGPF
AYGDQPTLADLCIVPQVFNARRFDADLAPFERIRQIEAEAMKLDAFVAAEPGRQPDAE
>Mature_217_residues
TKTADHDAAVLYGYFRSSAAYRVRIALNLKGVVVAQRYVHLRDGEQNLEAYRWINPAGSVPFWREGDFDLAQSLAIIEYL
DETHPEPPLLPRDPRARAIVREMAYAVACDIHPLGNLRILKRLTELGIDEIERARWSKQWIEQGFVAIEARLSQTPGPFA
YGDQPTLADLCIVPQVFNARRFDADLAPFERIRQIEAEAMKLDAFVAAEPGRQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=209, Percent_Identity=43.5406698564593, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI22202622, Length=157, Percent_Identity=43.9490445859873, Blast_Score=130, Evalue=7e-31,
Organism=Homo sapiens, GI194440732, Length=209, Percent_Identity=32.5358851674641, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17551302, Length=209, Percent_Identity=42.5837320574163, Blast_Score=157, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17510461, Length=213, Percent_Identity=37.0892018779343, Blast_Score=128, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556142, Length=210, Percent_Identity=36.1904761904762, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355859, Length=209, Percent_Identity=42.5837320574163, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24645375, Length=209, Percent_Identity=42.5837320574163, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI45553325, Length=212, Percent_Identity=41.9811320754717, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21355857, Length=210, Percent_Identity=39.0476190476191, Blast_Score=160, Evalue=6e-40,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24717; Mature: 24586

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTADHDAAVLYGYFRSSAAYRVRIALNLKGVVVAQRYVHLRDGEQNLEAYRWINPAGS
CCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCEEEHHHHHHCCCCCHHHHHHEEECCCCC
VPFWREGDFDLAQSLAIIEYLDETHPEPPLLPRDPRARAIVREMAYAVACDIHPLGNLRI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
LKRLTELGIDEIERARWSKQWIEQGFVAIEARLSQTPGPFAYGDQPTLADLCIVPQVFNA
HHHHHHCCHHHHHHHHHHHHHHHCCHHEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHCC
RRFDADLAPFERIRQIEAEAMKLDAFVAAEPGRQPDAE
HHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
>Mature Secondary Structure 
TKTADHDAAVLYGYFRSSAAYRVRIALNLKGVVVAQRYVHLRDGEQNLEAYRWINPAGS
CCCCCCCHHHHHHHHHCCCEEEEEEEEECCCEEEHHHHHHCCCCCHHHHHHEEECCCCC
VPFWREGDFDLAQSLAIIEYLDETHPEPPLLPRDPRARAIVREMAYAVACDIHPLGNLRI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
LKRLTELGIDEIERARWSKQWIEQGFVAIEARLSQTPGPFAYGDQPTLADLCIVPQVFNA
HHHHHHCCHHHHHHHHHHHHHHHCCHHEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHCC
RRFDADLAPFERIRQIEAEAMKLDAFVAAEPGRQPDAE
HHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]