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The map label for this gene is artI [H]

Identifier: 86748013

GI number: 86748013

Start: 1015634

End: 1016506

Strand: Direct

Name: artI [H]

Synonym: RPB_0887

Alternate gene names: 86748013

Gene position: 1015634-1016506 (Clockwise)

Preceding gene: 86748011

Following gene: 86748014

Centisome position: 19.05

GC content: 66.78

Gene sequence:

>873_bases
ATGCGGCCATTGCTGATGGCGCTGATGCTGTTCGGTGCTCTGCTGCCGCTGTGCGACGCGCACGCGGCCGAGACCGGCGC
GGAAGCCAAGGCCGTCGCGGCGGCGACTGCGCATGCCGTGCCGGGATTCTGGGACCCGCGGCGGCGCCCGGATCGGCCCG
ACCTGTCGCGCCTCACCGTGATCCGGTTTCTCACCGAGACCGACTATCCGCCGTTCAATTTCACCGGCGCCGACGGCAAT
CCGGCCGGCTTCAATGTCGATCTGGCGCGGGCGCTGTGCGAGGAGATGAAGGTCACCTGCACGGTGCAGATGCGGCGGTT
CGAGACGCTGCTCGATGCGCTGGCGAGCAATCGCGGCGACGCCATCATCGCATCGCTGGCGGTGACGCCGCAGACCCGCG
GCAAGGTCGATTTCACCGATCCTTATTACCGCGCGCCCGGCCGTTTCGTCGCCCGCAAGGACGCGGTGCTGCCGGAAATC
CGCCCGGAATATCTCGAGGGCAAGAAGGTCGGCGCGATCGCCGGCTCCGCGCACGAGGCCTATCTCAAGGCAATGTTCAC
CGACGCCGAGTTGCATTCCTACCCGAACGACGAGGCGCTGCGGGCGGCGCTGCGGCGCGGCGAGGTCGACTTCATCTTCG
GCGACGCGATCTCGCTGGCGTTCTGGATCAACGGCACCGATTCCGAGAATTGCTGCGCCTTCAGCGGCGGTCCGTTCACC
GAAAGTCGCTATTTCGGCGAGGGCGTCGGCATCGCGGTCCGCAAGGGCAACGATCTGCTGCGCCAGTCGCTGAACTGGGC
GCTGTTCCGGGTCTGGGAGAAGGGCCGCTACACCGATCTGTGGCTGCGGTATTTTTCCGTCAGCCCGTTCTGA

Upstream 100 bases:

>100_bases
GTCTATAAGATCGCCGGTGCGGATCCCGACTATGCAACCATGGATTGAGTTCTTGATCAACGGCCGATCGGCCGTGGCGC
GGCCATTGTTCGGGGCCGTG

Downstream 100 bases:

>100_bases
CAAATTCGAAAGACCCGCCTCGCGCGGTTGGAGACCTTGATGACCACGACCGATCTGCCCAGCGCGTCCGACCTGCGCAC
GCTCGCCGAACAATCCAACG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRPLLMALMLFGALLPLCDAHAAETGAEAKAVAAATAHAVPGFWDPRRRPDRPDLSRLTVIRFLTETDYPPFNFTGADGN
PAGFNVDLARALCEEMKVTCTVQMRRFETLLDALASNRGDAIIASLAVTPQTRGKVDFTDPYYRAPGRFVARKDAVLPEI
RPEYLEGKKVGAIAGSAHEAYLKAMFTDAELHSYPNDEALRAALRRGEVDFIFGDAISLAFWINGTDSENCCAFSGGPFT
ESRYFGEGVGIAVRKGNDLLRQSLNWALFRVWEKGRYTDLWLRYFSVSPF

Sequences:

>Translated_290_residues
MRPLLMALMLFGALLPLCDAHAAETGAEAKAVAAATAHAVPGFWDPRRRPDRPDLSRLTVIRFLTETDYPPFNFTGADGN
PAGFNVDLARALCEEMKVTCTVQMRRFETLLDALASNRGDAIIASLAVTPQTRGKVDFTDPYYRAPGRFVARKDAVLPEI
RPEYLEGKKVGAIAGSAHEAYLKAMFTDAELHSYPNDEALRAALRRGEVDFIFGDAISLAFWINGTDSENCCAFSGGPFT
ESRYFGEGVGIAVRKGNDLLRQSLNWALFRVWEKGRYTDLWLRYFSVSPF
>Mature_290_residues
MRPLLMALMLFGALLPLCDAHAAETGAEAKAVAAATAHAVPGFWDPRRRPDRPDLSRLTVIRFLTETDYPPFNFTGADGN
PAGFNVDLARALCEEMKVTCTVQMRRFETLLDALASNRGDAIIASLAVTPQTRGKVDFTDPYYRAPGRFVARKDAVLPEI
RPEYLEGKKVGAIAGSAHEAYLKAMFTDAELHSYPNDEALRAALRRGEVDFIFGDAISLAFWINGTDSENCCAFSGGPFT
ESRYFGEGVGIAVRKGNDLLRQSLNWALFRVWEKGRYTDLWLRYFSVSPF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=36.8421052631579, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=31.5555555555556, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=29.4871794871795, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=29.6943231441048, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788228, Length=220, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787031, Length=225, Percent_Identity=27.5555555555556, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 32144; Mature: 32144

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLLMALMLFGALLPLCDAHAAETGAEAKAVAAATAHAVPGFWDPRRRPDRPDLSRLTV
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
IRFLTETDYPPFNFTGADGNPAGFNVDLARALCEEMKVTCTVQMRRFETLLDALASNRGD
HHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCC
AIIASLAVTPQTRGKVDFTDPYYRAPGRFVARKDAVLPEIRPEYLEGKKVGAIAGSAHEA
EEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCEEEEECCCHHHH
YLKAMFTDAELHSYPNDEALRAALRRGEVDFIFGDAISLAFWINGTDSENCCAFSGGPFT
HHHHHHCCHHHHCCCCHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCCCEEEECCCCCC
ESRYFGEGVGIAVRKGNDLLRQSLNWALFRVWEKGRYTDLWLRYFSVSPF
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHEEECCCCC
>Mature Secondary Structure
MRPLLMALMLFGALLPLCDAHAAETGAEAKAVAAATAHAVPGFWDPRRRPDRPDLSRLTV
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
IRFLTETDYPPFNFTGADGNPAGFNVDLARALCEEMKVTCTVQMRRFETLLDALASNRGD
HHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCC
AIIASLAVTPQTRGKVDFTDPYYRAPGRFVARKDAVLPEIRPEYLEGKKVGAIAGSAHEA
EEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCEEEEECCCHHHH
YLKAMFTDAELHSYPNDEALRAALRRGEVDFIFGDAISLAFWINGTDSENCCAFSGGPFT
HHHHHHCCHHHHCCCCHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCCCEEEECCCCCC
ESRYFGEGVGIAVRKGNDLLRQSLNWALFRVWEKGRYTDLWLRYFSVSPF
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]