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The map label for this gene is nac [H]

Identifier: 86748005

GI number: 86748005

Start: 1006406

End: 1007308

Strand: Reverse

Name: nac [H]

Synonym: RPB_0879

Alternate gene names: 86748005

Gene position: 1007308-1006406 (Counterclockwise)

Preceding gene: 86748006

Following gene: 86748004

Centisome position: 18.89

GC content: 69.44

Gene sequence:

>903_bases
ATGAATCTCAAGCAGCTCGAATATTTCATGCGCGTGGCCGAGCTCGGCTCGTTCAGCAAGGCGGCGATGATGCTCGACAT
CGCGCAGCCGGCGCTGAGCCGGCAGGTCCGTGGGCTGGAGACCGAACTCAATCAGCAATTGTTCCTGCGCAACGGCCGCG
GCGTGGCGCTGACGGAAGCGGGGCGCCGGCTGTTCGACCACAGCGTCGGCATCATGCAGATGGTGGCGCATGCGCGCGAG
GATCTCGGCGCCAGCCGCGACGCGCCGGTCGGCCGCGTCACCATCGGCCTGCCGCCGTCGATCGGACGCCAGCTCACGCT
GCCGCTGATCGACCGCTTCAAGCGCGAACTGCCGGCGGCGCGGCTCGCGATCGTCGAGGGGCTGTCGTCGCATATCGTCG
AATGGGTCACCACCGGCCGCGCCGATGTCGGGCTCGTGTACAATCCGGAAGCGCAATCCGGTATCGACATCACGCCGCTG
CTGCAGGAACCGCTCGGCCTCGTCAGCCGGGCCCCGAAGGGCAAGCGCGGCCAGCCGGCGCCGCCGCTGCCGTTCAAGGA
ACTGTCGGCCTATCCGCTGATCGTGCCGGAGCGCGTCCATGCGATGCGCCGGCTGCTCGAGACCCAGGCGGCGCTCGCCG
GGGTCAAGCTCGACATCGCCTGGGAAGTCTCGAGCGTGCCGTCGATCATCGAACTGGTCTGCGCCGGCTACGGCCATGCG
GTACTGACCGCGAGCGGCGTCGCCGCCTCGCCGCGCGCGGCCGAACTGAGTTCGCGCAGGCTGATAGACCCCGCGGCGAC
CAGCGCGCTGTGCCTCGCGGTGTCCGCGACGAGACGGCCGACGCCGCTCGCGCAATATACCATGCGGCTGCTGACGGAAC
TGGCGCGCAGCCTGCCGAACTGA

Upstream 100 bases:

>100_bases
GCTCGCGACGCGACTGCGCGCCGACATCGAGAAATGGAAGGCCGTGATCGAGACCTCCGGGATCGAGAAACAATAGCGGC
CCGTCCGGGCAAGGCATTCG

Downstream 100 bases:

>100_bases
GCGCGCCGCGCGTTGCAGTGCTGGGGCGGCATGGCGGCACGACTTTGCCATACCTTAACCCGATAGCTGTTATTGAGACG
CGGCGGTGGCGTCGTCCGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNLKQLEYFMRVAELGSFSKAAMMLDIAQPALSRQVRGLETELNQQLFLRNGRGVALTEAGRRLFDHSVGIMQMVAHARE
DLGASRDAPVGRVTIGLPPSIGRQLTLPLIDRFKRELPAARLAIVEGLSSHIVEWVTTGRADVGLVYNPEAQSGIDITPL
LQEPLGLVSRAPKGKRGQPAPPLPFKELSAYPLIVPERVHAMRRLLETQAALAGVKLDIAWEVSSVPSIIELVCAGYGHA
VLTASGVAASPRAAELSSRRLIDPAATSALCLAVSATRRPTPLAQYTMRLLTELARSLPN

Sequences:

>Translated_300_residues
MNLKQLEYFMRVAELGSFSKAAMMLDIAQPALSRQVRGLETELNQQLFLRNGRGVALTEAGRRLFDHSVGIMQMVAHARE
DLGASRDAPVGRVTIGLPPSIGRQLTLPLIDRFKRELPAARLAIVEGLSSHIVEWVTTGRADVGLVYNPEAQSGIDITPL
LQEPLGLVSRAPKGKRGQPAPPLPFKELSAYPLIVPERVHAMRRLLETQAALAGVKLDIAWEVSSVPSIIELVCAGYGHA
VLTASGVAASPRAAELSSRRLIDPAATSALCLAVSATRRPTPLAQYTMRLLTELARSLPN
>Mature_300_residues
MNLKQLEYFMRVAELGSFSKAAMMLDIAQPALSRQVRGLETELNQQLFLRNGRGVALTEAGRRLFDHSVGIMQMVAHARE
DLGASRDAPVGRVTIGLPPSIGRQLTLPLIDRFKRELPAARLAIVEGLSSHIVEWVTTGRADVGLVYNPEAQSGIDITPL
LQEPLGLVSRAPKGKRGQPAPPLPFKELSAYPLIVPERVHAMRRLLETQAALAGVKLDIAWEVSSVPSIIELVCAGYGHA
VLTASGVAASPRAAELSSRRLIDPAATSALCLAVSATRRPTPLAQYTMRLLTELARSLPN

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=298, Percent_Identity=29.1946308724832, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693105, Length=259, Percent_Identity=32.046332046332, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI157672245, Length=232, Percent_Identity=28.448275862069, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790399, Length=250, Percent_Identity=28.4, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788887, Length=169, Percent_Identity=33.1360946745562, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=154, Percent_Identity=29.8701298701299, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=24.6913580246914, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=261, Percent_Identity=27.9693486590038, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786401, Length=159, Percent_Identity=31.4465408805031, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789639, Length=126, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789506, Length=232, Percent_Identity=24.1379310344828, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789204, Length=257, Percent_Identity=29.1828793774319, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=172, Percent_Identity=27.3255813953488, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=260, Percent_Identity=24.2307692307692, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367136, Length=127, Percent_Identity=32.2834645669291, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789440, Length=192, Percent_Identity=27.0833333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=130, Percent_Identity=30, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32411; Mature: 32411

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQLEYFMRVAELGSFSKAAMMLDIAQPALSRQVRGLETELNQQLFLRNGRGVALTEA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GRRLFDHSVGIMQMVAHAREDLGASRDAPVGRVTIGLPPSIGRQLTLPLIDRFKRELPAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHCCHH
RLAIVEGLSSHIVEWVTTGRADVGLVYNPEAQSGIDITPLLQEPLGLVSRAPKGKRGQPA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PPLPFKELSAYPLIVPERVHAMRRLLETQAALAGVKLDIAWEVSSVPSIIELVCAGYGHA
CCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHCCCCC
VLTASGVAASPRAAELSSRRLIDPAATSALCLAVSATRRPTPLAQYTMRLLTELARSLPN
EEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLKQLEYFMRVAELGSFSKAAMMLDIAQPALSRQVRGLETELNQQLFLRNGRGVALTEA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GRRLFDHSVGIMQMVAHAREDLGASRDAPVGRVTIGLPPSIGRQLTLPLIDRFKRELPAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHCCHH
RLAIVEGLSSHIVEWVTTGRADVGLVYNPEAQSGIDITPLLQEPLGLVSRAPKGKRGQPA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PPLPFKELSAYPLIVPERVHAMRRLLETQAALAGVKLDIAWEVSSVPSIIELVCAGYGHA
CCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHCCCCC
VLTASGVAASPRAAELSSRRLIDPAATSALCLAVSATRRPTPLAQYTMRLLTELARSLPN
EEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]