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The map label for this gene is spo0F [H]

Identifier: 86747958

GI number: 86747958

Start: 947541

End: 947918

Strand: Reverse

Name: spo0F [H]

Synonym: RPB_0832

Alternate gene names: 86747958

Gene position: 947918-947541 (Counterclockwise)

Preceding gene: 86747959

Following gene: 86747956

Centisome position: 17.78

GC content: 66.93

Gene sequence:

>378_bases
ATGGCGCGAATCCTGATCGCCGACGACGAAGAATCGATGCGCCTCCTGGTGGCGCGCGCCATCGGCATGGACGGCCACGA
GACCGTCACCGCCCAGGACGGCAGCGAGGCGCTGGAAATCCTGATCGAGAGTGCCGGCCGGTTCGATCTGTTGCTGACCG
ACATCAAGATGCCGATCATGGACGGCATCGCGCTGGCGCTGGCGGCGGCGCGCGACTTCCCCGATCTCACCATCCTGCTG
ATGACCGGTTTCGCCGATCAGCGCGAACGCGCCTCCGGGCTCGACGCCCTGATCCACGACGTCATCACCAAGCCTTTCTC
GGTGGCCGACATCCGCAACGCGGTGGCCGCCGCGCTGGCGGCGAAGAAGACGGGCTGA

Upstream 100 bases:

>100_bases
TTCTTCAACCGGCGCGATATGATGGCCGGGCGCGCGTGACGCCGCCCGGATCCGTCCGCACGCCGGCCGACCGCACCACC
GCGCAAACTAGGACCGAGTG

Downstream 100 bases:

>100_bases
TCACCCAGCGCCGGGCCGCAGCCTGCCCCCTCTCCCGCCTGCGGGAGAGGGCCGGGGTGAGGGCGACGCGGGCAGTGACT
CAGCCACTCGTTGAGAGGAG

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MARILIADDEESMRLLVARAIGMDGHETVTAQDGSEALEILIESAGRFDLLLTDIKMPIMDGIALALAAARDFPDLTILL
MTGFADQRERASGLDALIHDVITKPFSVADIRNAVAAALAAKKTG

Sequences:

>Translated_125_residues
MARILIADDEESMRLLVARAIGMDGHETVTAQDGSEALEILIESAGRFDLLLTDIKMPIMDGIALALAAARDFPDLTILL
MTGFADQRERASGLDALIHDVITKPFSVADIRNAVAAALAAKKTG
>Mature_124_residues
ARILIADDEESMRLLVARAIGMDGHETVTAQDGSEALEILIESAGRFDLLLTDIKMPIMDGIALALAAARDFPDLTILLM
TGFADQRERASGLDALIHDVITKPFSVADIRNAVAAALAAKKTG

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=35.3982300884956, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=35.5932203389831, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13320; Mature: 13188

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILIADDEESMRLLVARAIGMDGHETVTAQDGSEALEILIESAGRFDLLLTDIKMPIM
CCEEEEECCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHHH
DGIALALAAARDFPDLTILLMTGFADQRERASGLDALIHDVITKPFSVADIRNAVAAALA
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AKKTG
HHCCC
>Mature Secondary Structure 
ARILIADDEESMRLLVARAIGMDGHETVTAQDGSEALEILIESAGRFDLLLTDIKMPIM
CEEEEECCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHHH
DGIALALAAARDFPDLTILLMTGFADQRERASGLDALIHDVITKPFSVADIRNAVAAALA
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AKKTG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3003689; 2997779; 2457578; 9353933; 9384377; 8528078; 9254596; 8805550; 9335530; 10997904 [H]