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The map label for this gene is caiD [H]

Identifier: 86747827

GI number: 86747827

Start: 787211

End: 787996

Strand: Reverse

Name: caiD [H]

Synonym: RPB_0701

Alternate gene names: 86747827

Gene position: 787996-787211 (Counterclockwise)

Preceding gene: 86747828

Following gene: 86747824

Centisome position: 14.78

GC content: 71.12

Gene sequence:

>786_bases
ATGCCGACCCAGAACACGGTGCTGATCGAGCGCGACGGCCCGGTGACGATCGTGTCGATCAACCGCCCCGCGCGCCGCAA
CGCGGTCGACGGCGCCACCGCGAAGAAGCTCTACGACGCCTTCCTGGCGTTCGATGCCGACGACACCTCGTCCGTCGCGG
TGTTCACCGGCGCGGGCGGCACGTTCTGCGCCGGCGCCGACCTCAAGGCGGTGGCGGCGGGCGACAGCGAGCGATCGCGG
GACATCGCCGGCCACGACACGCTGGCGCCGATGGGCCCGACCCGGCTGCGGCTGGGCAAGCCGGTGATCGCCGCGGTCGA
AGGCCATGCGGTCGCCGGCGGCCTCGAACTGGCGCTGTGGGCGGACATGCGGGTCGCCGCCGAGGACGCCACCTTCGGCG
TGTTCTGCCGCCGCTTCGGCGTGCCGCTGATCGACGGCGGCACCATCAGGCTGCCGCGGCTGATCGGCCATTCGCAGGCG
ATGGACATGATCCTCACCGGCCGCCCGGTCTCCGGCATCGAGGCGCAGCGGATGGGCCTCGCCAACCGCCTGGTCCACCG
CGGCGAAGCCGCGCTGCACGCGATCACGCTCGCCAAGGAGATCGCGCACTTCCCGCAGACCTGTCTGCGCGCCGACCGGC
TGTCGGCGCTGCAGCAATGGGACATGCCGGAAGACGACGCGATCATGAACGAGATGCGCGGCGGCCTGAAAGTCATCGCC
ACTGGCGAAACGCTCGAAGGCGCCGAACGCTTCACCCAGGGTGAAGGCCGGCACGGCAAGTTCTGA

Upstream 100 bases:

>100_bases
CGGGCGCCGATCTCGCGATCTCCTGACATGACGTAGCGCAAGGCGGCGCCGGGTGCGGCGGGCATTGATCGCCCGAGATG
ACTGCCTCTGGAGAAGACCC

Downstream 100 bases:

>100_bases
GCGGGCGGAGCAGCGAAGCCAACTCGCCGCGCTTACCCGTCATCCTGAGGTGCGCGCTCTTGCGCGCCTCGAAGGATGAG
CCGCAGGCACCGCGCTTGAC

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MPTQNTVLIERDGPVTIVSINRPARRNAVDGATAKKLYDAFLAFDADDTSSVAVFTGAGGTFCAGADLKAVAAGDSERSR
DIAGHDTLAPMGPTRLRLGKPVIAAVEGHAVAGGLELALWADMRVAAEDATFGVFCRRFGVPLIDGGTIRLPRLIGHSQA
MDMILTGRPVSGIEAQRMGLANRLVHRGEAALHAITLAKEIAHFPQTCLRADRLSALQQWDMPEDDAIMNEMRGGLKVIA
TGETLEGAERFTQGEGRHGKF

Sequences:

>Translated_261_residues
MPTQNTVLIERDGPVTIVSINRPARRNAVDGATAKKLYDAFLAFDADDTSSVAVFTGAGGTFCAGADLKAVAAGDSERSR
DIAGHDTLAPMGPTRLRLGKPVIAAVEGHAVAGGLELALWADMRVAAEDATFGVFCRRFGVPLIDGGTIRLPRLIGHSQA
MDMILTGRPVSGIEAQRMGLANRLVHRGEAALHAITLAKEIAHFPQTCLRADRLSALQQWDMPEDDAIMNEMRGGLKVIA
TGETLEGAERFTQGEGRHGKF
>Mature_260_residues
PTQNTVLIERDGPVTIVSINRPARRNAVDGATAKKLYDAFLAFDADDTSSVAVFTGAGGTFCAGADLKAVAAGDSERSRD
IAGHDTLAPMGPTRLRLGKPVIAAVEGHAVAGGLELALWADMRVAAEDATFGVFCRRFGVPLIDGGTIRLPRLIGHSQAM
DMILTGRPVSGIEAQRMGLANRLVHRGEAALHAITLAKEIAHFPQTCLRADRLSALQQWDMPEDDAIMNEMRGGLKVIAT
GETLEGAERFTQGEGRHGKF

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=30.3149606299213, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI4757966, Length=200, Percent_Identity=26, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI51538581, Length=200, Percent_Identity=26, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI25453481, Length=200, Percent_Identity=26, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI51538573, Length=200, Percent_Identity=26, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=31.3186813186813, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757968, Length=200, Percent_Identity=26, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI49355787, Length=200, Percent_Identity=26, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=205, Percent_Identity=31.219512195122, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI70995211, Length=189, Percent_Identity=32.8042328042328, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI31542718, Length=225, Percent_Identity=28, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI221142681, Length=254, Percent_Identity=33.0708661417323, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=33.2, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=29.2682926829268, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787660, Length=193, Percent_Identity=32.1243523316062, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=29.0155440414508, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790281, Length=189, Percent_Identity=28.042328042328, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=256, Percent_Identity=48.046875, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17540714, Length=189, Percent_Identity=32.8042328042328, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=30.1652892561983, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=198, Percent_Identity=32.8282828282828, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=29.1828793774319, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=227, Percent_Identity=25.9911894273128, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=231, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=28.3422459893048, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=191, Percent_Identity=28.2722513089005, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=191, Percent_Identity=28.2722513089005, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=191, Percent_Identity=28.2722513089005, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=241, Percent_Identity=27.3858921161826, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=258, Percent_Identity=24.4186046511628, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=258, Percent_Identity=24.4186046511628, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17549921, Length=184, Percent_Identity=28.804347826087, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=185, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357171, Length=252, Percent_Identity=44.8412698412698, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24650670, Length=255, Percent_Identity=42.3529411764706, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=31.5789473684211, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=31.5789473684211, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=33.7209302325581, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24654903, Length=183, Percent_Identity=23.4972677595628, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19921000, Length=180, Percent_Identity=29.4444444444444, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583077, Length=180, Percent_Identity=29.4444444444444, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583079, Length=180, Percent_Identity=29.4444444444444, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27768; Mature: 27637

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQNTVLIERDGPVTIVSINRPARRNAVDGATAKKLYDAFLAFDADDTSSVAVFTGAGG
CCCCCCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEEECCCC
TFCAGADLKAVAAGDSERSRDIAGHDTLAPMGPTRLRLGKPVIAAVEGHAVAGGLELALW
CEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCEEECCEEEEEE
ADMRVAAEDATFGVFCRRFGVPLIDGGTIRLPRLIGHSQAMDMILTGRPVSGIEAQRMGL
ECEEEECCCCHHHHHHHHHCCCEECCCCEEEHHHHCCHHHHHEEEECCCCCCCHHHHHHH
ANRLVHRGEAALHAITLAKEIAHFPQTCLRADRLSALQQWDMPEDDAIMNEMRGGLKVIA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEE
TGETLEGAERFTQGEGRHGKF
ECCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PTQNTVLIERDGPVTIVSINRPARRNAVDGATAKKLYDAFLAFDADDTSSVAVFTGAGG
CCCCCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEEECCCC
TFCAGADLKAVAAGDSERSRDIAGHDTLAPMGPTRLRLGKPVIAAVEGHAVAGGLELALW
CEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCEEECCEEEEEE
ADMRVAAEDATFGVFCRRFGVPLIDGGTIRLPRLIGHSQAMDMILTGRPVSGIEAQRMGL
ECEEEECCCCHHHHHHHHHCCCEECCCCEEEHHHHCCHHHHHEEEECCCCCCCHHHHHHH
ANRLVHRGEAALHAITLAKEIAHFPQTCLRADRLSALQQWDMPEDDAIMNEMRGGLKVIA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEE
TGETLEGAERFTQGEGRHGKF
ECCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA