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The map label for this gene is dapE

Identifier: 86747802

GI number: 86747802

Start: 760634

End: 761803

Strand: Reverse

Name: dapE

Synonym: RPB_0676

Alternate gene names: 86747802

Gene position: 761803-760634 (Counterclockwise)

Preceding gene: 162138523

Following gene: 86747798

Centisome position: 14.29

GC content: 67.44

Gene sequence:

>1170_bases
GTGACCGCGACCGCGCTCGAGATCGCGCAGGCGCTGCTGCGCTGTCCGTCGGTGACGCCGGCCGATGCCGGCGCGCTCGG
CGTGCTGGAGGCGCTGCTGCAGGACGCGGGCTTCGCGACGCATCGGGTGACTTTCAGCGAGCCCGGCACCGCCGACATCG
ACAATCTCTACGCGCGGATCGGTGACGGTGCGCCGCATCTCTGCTTCGCCGGCCACACCGACGTGGTGCCGCCCGGCGAA
GAAACCGCATGGAGCCATGGCGCGTTCGCAGGCGATGTCGAAGGCGGCCTGCTGTACGGCCGCGGCGCGGTCGACATGAA
GGGCGGCATCGCCTGCGCGGTGGCGGCGACGCTGGATTATCTGGCCGCGAATGGCGGCCAGCCGCAACAATCCGGCAAAG
GCTCGATCTCGTTCCTGATCACCGGCGACGAGGAAGACGTCGCCGTCAACGGCACCGTCAAGCTGCTGCAATGGGCCGCC
GAACGCGGCGAGCGCTTCGATCACTGCATCGTCGGCGAGCCGAGCAATGTCGAGGCGATCGGCGACACCATCAAGATCGG
TCGGCGCGGCTCGCAGTCCGGCGTGCTGATCGTCGACGGCCGGCAGGGCCATGTCGCCTATCCGCACCGCGCATCGAATC
CGATCCCCGATATCGCAACGTTGATCACCGCGCTGAACGACGAGCCGCTGGATCAAGGCTCGGCGCAATTCCAGCCGTCG
AACCTCGAATTCACCTCGGTCGACGTCGGCAATCCGGCCACCAATGTGATCCCGGCGCAGGCCCGCGCCAAGTTCAACAT
CCGCTTCAACGATCACCACACCCAGGAGACGCTGCGCGCGCTGGTCGAGCAGCGGCTCGCCGCCGCCTGCGGCAACCGCA
TCCGCGCCCGAATCGAATGGCTGCCGTCGAACGCCGATGTGTTCGTGACGAAGCCCGGCGGCTTCACCGATCTGGTGTCG
GCCGCGATCGCCGACATCACCGGCCGCAGCCCCGACCTCAACACCGGCGGCGGCACCTCGGATGCGCGCTTCATCGCCAA
ATACTGTCCGGTGGTCGAGTTCGGCCTGGTCGGCCAGACCATGCACCAGATCGACGAACGCACGCCCGTGAGCGATCTCG
ACAAGCTCACCGCGATCTATCGCGGCGTGCTGGAGAGATACTTCAGGTAA

Upstream 100 bases:

>100_bases
CCGGGCCCGGCCACCGCCTGCGCGGTGATCGTCAAGCGCGTCGACGAGCGCACCCGCTCCAAGACCAGCATCAACGAATT
GCTGCGGGACTGATCGCGCC

Downstream 100 bases:

>100_bases
ACGACCTCTCTTTCGTCATTCCGGGGCGCGCGTAGCGCGAGCCCGGAATCCATACTCACTGTCAGGGGTTATGGATTCCG
GGCCTGTGCCGCTGCGCGGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTATALEIAQALLRCPSVTPADAGALGVLEALLQDAGFATHRVTFSEPGTADIDNLYARIGDGAPHLCFAGHTDVVPPGE
ETAWSHGAFAGDVEGGLLYGRGAVDMKGGIACAVAATLDYLAANGGQPQQSGKGSISFLITGDEEDVAVNGTVKLLQWAA
ERGERFDHCIVGEPSNVEAIGDTIKIGRRGSQSGVLIVDGRQGHVAYPHRASNPIPDIATLITALNDEPLDQGSAQFQPS
NLEFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLRALVEQRLAAACGNRIRARIEWLPSNADVFVTKPGGFTDLVS
AAIADITGRSPDLNTGGGTSDARFIAKYCPVVEFGLVGQTMHQIDERTPVSDLDKLTAIYRGVLERYFR

Sequences:

>Translated_389_residues
MTATALEIAQALLRCPSVTPADAGALGVLEALLQDAGFATHRVTFSEPGTADIDNLYARIGDGAPHLCFAGHTDVVPPGE
ETAWSHGAFAGDVEGGLLYGRGAVDMKGGIACAVAATLDYLAANGGQPQQSGKGSISFLITGDEEDVAVNGTVKLLQWAA
ERGERFDHCIVGEPSNVEAIGDTIKIGRRGSQSGVLIVDGRQGHVAYPHRASNPIPDIATLITALNDEPLDQGSAQFQPS
NLEFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLRALVEQRLAAACGNRIRARIEWLPSNADVFVTKPGGFTDLVS
AAIADITGRSPDLNTGGGTSDARFIAKYCPVVEFGLVGQTMHQIDERTPVSDLDKLTAIYRGVLERYFR
>Mature_388_residues
TATALEIAQALLRCPSVTPADAGALGVLEALLQDAGFATHRVTFSEPGTADIDNLYARIGDGAPHLCFAGHTDVVPPGEE
TAWSHGAFAGDVEGGLLYGRGAVDMKGGIACAVAATLDYLAANGGQPQQSGKGSISFLITGDEEDVAVNGTVKLLQWAAE
RGERFDHCIVGEPSNVEAIGDTIKIGRRGSQSGVLIVDGRQGHVAYPHRASNPIPDIATLITALNDEPLDQGSAQFQPSN
LEFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLRALVEQRLAAACGNRIRARIEWLPSNADVFVTKPGGFTDLVSA
AIADITGRSPDLNTGGGTSDARFIAKYCPVVEFGLVGQTMHQIDERTPVSDLDKLTAIYRGVLERYFR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=387, Percent_Identity=40.0516795865633, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1790395, Length=217, Percent_Identity=29.4930875576037, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_RHOP2 (Q2J2C3)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484298.1
- ProteinModelPortal:   Q2J2C3
- STRING:   Q2J2C3
- MEROPS:   M20.010
- GeneID:   3908603
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0676
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- ProtClustDB:   PRK13009
- BioCyc:   RPAL316058:RPB_0676-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41170; Mature: 41038

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 74-74 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATALEIAQALLRCPSVTPADAGALGVLEALLQDAGFATHRVTFSEPGTADIDNLYARI
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
GDGAPHLCFAGHTDVVPPGEETAWSHGAFAGDVEGGLLYGRGAVDMKGGIACAVAATLDY
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
LAANGGQPQQSGKGSISFLITGDEEDVAVNGTVKLLQWAAERGERFDHCIVGEPSNVEAI
HHCCCCCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHCCCCCEEECCCCCCCHHH
GDTIKIGRRGSQSGVLIVDGRQGHVAYPHRASNPIPDIATLITALNDEPLDQGSAQFQPS
CHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
NLEFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLRALVEQRLAAACGNRIRARIEW
CCEEEEEECCCCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHEEEEEEE
LPSNADVFVTKPGGFTDLVSAAIADITGRSPDLNTGGGTSDARFIAKYCPVVEFGLVGQT
ECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
MHQIDERTPVSDLDKLTAIYRGVLERYFR
HHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TATALEIAQALLRCPSVTPADAGALGVLEALLQDAGFATHRVTFSEPGTADIDNLYARI
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
GDGAPHLCFAGHTDVVPPGEETAWSHGAFAGDVEGGLLYGRGAVDMKGGIACAVAATLDY
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
LAANGGQPQQSGKGSISFLITGDEEDVAVNGTVKLLQWAAERGERFDHCIVGEPSNVEAI
HHCCCCCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHCCCCCEEECCCCCCCHHH
GDTIKIGRRGSQSGVLIVDGRQGHVAYPHRASNPIPDIATLITALNDEPLDQGSAQFQPS
CHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
NLEFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLRALVEQRLAAACGNRIRARIEW
CCEEEEEECCCCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHEEEEEEE
LPSNADVFVTKPGGFTDLVSAAIADITGRSPDLNTGGGTSDARFIAKYCPVVEFGLVGQT
ECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
MHQIDERTPVSDLDKLTAIYRGVLERYFR
HHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA