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The map label for this gene is gyaR [H]

Identifier: 86747742

GI number: 86747742

Start: 694249

End: 695250

Strand: Direct

Name: gyaR [H]

Synonym: RPB_0616

Alternate gene names: 86747742

Gene position: 694249-695250 (Clockwise)

Preceding gene: 86747740

Following gene: 86747744

Centisome position: 13.02

GC content: 66.67

Gene sequence:

>1002_bases
ATGTCGGTGAAGAAAAAGCCTCTGGTCGTCGTCACCCGCAAGCTTCCGGACTCGATCGAAACCCGGATGCGTGAACTGTT
CGACGCGCGGCTCAATCTCGACGACGCGCCGATGTCGGCCGAGCAGCTCGCCGACGCCGCGCGCACCGCCGACGTGCTGG
TGCCGACCGTCACCGATTCGATCACCAGCGAGATGTTGAATCAGCCCGACTGCAAGCTGCGGCTGATCGCGAATTTCGGC
AACGGCATCGACAATATCGACGTCGCCGCCGCCCATGCCCGCGGCATCACCGTCACCAACACGCCGAAGGTGCTCACCGA
AGACACCGCCGACATGACGATGGCGCTGATTCTCGCGGTGCCGCGCCGGCTGATCGAAGGCGCCGCATTGCTCACCGACG
GCGGCGAATGGCCCGGCTGGTCGCCGACCTGGATGCTCGGCCGCCGTCTCGGCGGCAAGCGCCTCGGCATCATCGGCATG
GGCCGGATCGGCCAGGCGGTGGCGCGCCGCGCCCGCGCCTTCGGGCTGCAGATCCATTATCACAACCGCAAGCCGGTGGC
GCCGCGGATCGCCGACGAACTCGGCGCGACCTATTGGGATTCGCTCGACCAGATGCTGGCGCGGATGGACATCATCTCGG
TGAACTGTCCGCACACGCCCGCGACCTTCCATCTCTTGTCGGCGCGCCGTCTGAAGCTGGTCCGCAAGGACGCCTTCATC
GTCAACACCGCGCGCGGCGAGGTGATCGACGAGGAGACGCTGACCCGGCTGATCGAGAGCGGCGACATCGCCGGCGCCGG
CCTCGACGTCTACGAGCACGAGCCCGCGGTCAATCCGAAGCTGGTGCGCCTCGCCAAGCACGGCAAGGTGGTGCTGCTGC
CGCATATGGGCTCGGCGACGATCGAAGGCCGCGTCGAGATGGGCGAGAAGGTGATCATCAACATCCGCACCTTCCTCGAC
AACCACAAGCCGCCGGATCGCGTGCTGCCGGGGATGCTGTAG

Upstream 100 bases:

>100_bases
GGCCGACTTGGCCCCCTGCGGTCGCGCTGCAGCGGGAGTGTCGAACAGCTGGGTTAATGCGGACTAAACAGTCTCGCCAG
ACTCGTCAGAGAGCGGTTGA

Downstream 100 bases:

>100_bases
CCGCGCTCGACTCCACCGCCGTGCCCGCGAGCCCTCACCCCAACCCTCTCCCGCAAGCGGGCGAGGGGGCTCGCCGTGCG
CGTTGCTGAGTTTGAGACTG

Product: glycolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSVKKKPLVVVTRKLPDSIETRMRELFDARLNLDDAPMSAEQLADAARTADVLVPTVTDSITSEMLNQPDCKLRLIANFG
NGIDNIDVAAAHARGITVTNTPKVLTEDTADMTMALILAVPRRLIEGAALLTDGGEWPGWSPTWMLGRRLGGKRLGIIGM
GRIGQAVARRARAFGLQIHYHNRKPVAPRIADELGATYWDSLDQMLARMDIISVNCPHTPATFHLLSARRLKLVRKDAFI
VNTARGEVIDEETLTRLIESGDIAGAGLDVYEHEPAVNPKLVRLAKHGKVVLLPHMGSATIEGRVEMGEKVIINIRTFLD
NHKPPDRVLPGML

Sequences:

>Translated_333_residues
MSVKKKPLVVVTRKLPDSIETRMRELFDARLNLDDAPMSAEQLADAARTADVLVPTVTDSITSEMLNQPDCKLRLIANFG
NGIDNIDVAAAHARGITVTNTPKVLTEDTADMTMALILAVPRRLIEGAALLTDGGEWPGWSPTWMLGRRLGGKRLGIIGM
GRIGQAVARRARAFGLQIHYHNRKPVAPRIADELGATYWDSLDQMLARMDIISVNCPHTPATFHLLSARRLKLVRKDAFI
VNTARGEVIDEETLTRLIESGDIAGAGLDVYEHEPAVNPKLVRLAKHGKVVLLPHMGSATIEGRVEMGEKVIINIRTFLD
NHKPPDRVLPGML
>Mature_332_residues
SVKKKPLVVVTRKLPDSIETRMRELFDARLNLDDAPMSAEQLADAARTADVLVPTVTDSITSEMLNQPDCKLRLIANFGN
GIDNIDVAAAHARGITVTNTPKVLTEDTADMTMALILAVPRRLIEGAALLTDGGEWPGWSPTWMLGRRLGGKRLGIIGMG
RIGQAVARRARAFGLQIHYHNRKPVAPRIADELGATYWDSLDQMLARMDIISVNCPHTPATFHLLSARRLKLVRKDAFIV
NTARGEVIDEETLTRLIESGDIAGAGLDVYEHEPAVNPKLVRLAKHGKVVLLPHMGSATIEGRVEMGEKVIINIRTFLDN
HKPPDRVLPGML

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=303, Percent_Identity=35.6435643564356, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI23308577, Length=311, Percent_Identity=31.1897106109325, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI61743967, Length=251, Percent_Identity=34.2629482071713, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557497, Length=251, Percent_Identity=34.2629482071713, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI145580578, Length=254, Percent_Identity=34.251968503937, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557499, Length=254, Percent_Identity=34.251968503937, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI145580575, Length=254, Percent_Identity=34.251968503937, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI87082289, Length=313, Percent_Identity=36.4217252396166, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789279, Length=292, Percent_Identity=30.1369863013699, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787645, Length=336, Percent_Identity=24.702380952381, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17532191, Length=268, Percent_Identity=32.089552238806, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25147481, Length=204, Percent_Identity=34.8039215686275, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=247, Percent_Identity=36.8421052631579, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6320925, Length=271, Percent_Identity=30.2583025830258, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=269, Percent_Identity=28.996282527881, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324964, Length=219, Percent_Identity=30.1369863013699, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=153, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=264, Percent_Identity=26.5151515151515, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324980, Length=149, Percent_Identity=29.5302013422819, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28571528, Length=289, Percent_Identity=37.7162629757785, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19921140, Length=271, Percent_Identity=32.1033210332103, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24646446, Length=287, Percent_Identity=33.7979094076655, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646448, Length=287, Percent_Identity=33.7979094076655, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646452, Length=287, Percent_Identity=33.7979094076655, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646450, Length=287, Percent_Identity=33.7979094076655, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI62472511, Length=248, Percent_Identity=36.6935483870968, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45552429, Length=279, Percent_Identity=30.8243727598566, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24585514, Length=278, Percent_Identity=30.9352517985612, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI28574282, Length=278, Percent_Identity=30.9352517985612, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI28574286, Length=310, Percent_Identity=31.2903225806452, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI28574284, Length=278, Percent_Identity=30.9352517985612, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45551003, Length=278, Percent_Identity=30.9352517985612, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24585516, Length=273, Percent_Identity=27.4725274725275, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 36568; Mature: 36437

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKKKPLVVVTRKLPDSIETRMRELFDARLNLDDAPMSAEQLADAARTADVLVPTVTDS
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEHHHHH
ITSEMLNQPDCKLRLIANFGNGIDNIDVAAAHARGITVTNTPKVLTEDTADMTMALILAV
HHHHHHCCCCCEEEEEEECCCCCCCCCEEEHHCCCEEECCCCCEEECCHHHHHHHHHHHH
PRRLIEGAALLTDGGEWPGWSPTWMLGRRLGGKRLGIIGMGRIGQAVARRARAFGLQIHY
HHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHEEEEEE
HNRKPVAPRIADELGATYWDSLDQMLARMDIISVNCPHTPATFHLLSARRLKLVRKDAFI
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCEEE
VNTARGEVIDEETLTRLIESGDIAGAGLDVYEHEPAVNPKLVRLAKHGKVVLLPHMGSAT
EECCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEECCCCEEEEECCCCCE
IEGRVEMGEKVIINIRTFLDNHKPPDRVLPGML
ECCHHCCCCEEEEEEEHHHCCCCCHHHCCCCCC
>Mature Secondary Structure 
SVKKKPLVVVTRKLPDSIETRMRELFDARLNLDDAPMSAEQLADAARTADVLVPTVTDS
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEHHHHH
ITSEMLNQPDCKLRLIANFGNGIDNIDVAAAHARGITVTNTPKVLTEDTADMTMALILAV
HHHHHHCCCCCEEEEEEECCCCCCCCCEEEHHCCCEEECCCCCEEECCHHHHHHHHHHHH
PRRLIEGAALLTDGGEWPGWSPTWMLGRRLGGKRLGIIGMGRIGQAVARRARAFGLQIHY
HHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHEEEEEE
HNRKPVAPRIADELGATYWDSLDQMLARMDIISVNCPHTPATFHLLSARRLKLVRKDAFI
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCEEE
VNTARGEVIDEETLTRLIESGDIAGAGLDVYEHEPAVNPKLVRLAKHGKVVLLPHMGSAT
EECCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEECCCCEEEEECCCCCE
IEGRVEMGEKVIINIRTFLDNHKPPDRVLPGML
ECCHHCCCCEEEEEEEHHHCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA