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The map label for this gene is nusA [H]

Identifier: 86747724

GI number: 86747724

Start: 670952

End: 672571

Strand: Direct

Name: nusA [H]

Synonym: RPB_0598

Alternate gene names: 86747724

Gene position: 670952-672571 (Clockwise)

Preceding gene: 86747723

Following gene: 86747725

Centisome position: 12.58

GC content: 66.3

Gene sequence:

>1620_bases
ATGGCCGTCAGCGCCAACAAGCTGGAATTGCTGCAGATCGCCGACGCGGTGGCGCGGGAGAAATCGATCGACCGCGGCAT
CGTGATCGCCGCGATGGAAGACGCGATCGCGAAGGCGGCGCGCGCCCGCTACGGCTCGGAGACCGACGTCCACGCCGAGA
TCGACGCCAAGAAGGGCGAATTGCGGCTGTCGCGCCATATGCTGGTGGTCGAAAACGTCGAGAACCCCGCCAACCAGATC
TCGCTGAAGGCCGCGCAGCGCGCCAACCCCGGCGCGCAGATCGGCGACACCATCGCCGACACGCTGCCGCCGCTGGAATA
CGGCCGCATCGCCGCGCAGTCGGCCAAGCAGGTGATCGTGCAGAAGGTGCGCGAGGCCGAGCGTGACCGGCAATACTCGG
AATTCAAGGATCGCATCGGCGACATCGTCAACGGCGTCGTCAAGCGCGTCGAATACGGCAGCGTGATCGTCGATCTCGGC
CGCGGCGAGGCGATCGTGCGCCGCGACGAGATGCTGCCGCGCGAATCGTTCCGCAACGGCGACCGCGTCCGCGCCTACAT
CTTCGACGTCCGCCGCGAGACCCGCGGCCCGCAGATCTTCCTGTCGCGCACTCATCCGCAGTTCATGGCCAAGCTGTTCG
CCCAGGAAGTGCCGGAAATCTACGACGGCATCGTCGAGATCAAGGCGGTCGCCCGCGATCCGGGCTCGCGCGCCAAGATC
GGCGTGGTGTCGCGGGATTCCTCGGTCGATCCGGTCGGCGCCTGCGTCGGCATGCGCGGCTCGCGCGTTCAGGCCGTGGT
CAACGAGCTGCAGGGCGAGAAGATCGACATCATCCCGTGGTCGCCGGACATCGCCACTTTCGTGGTCAACGCGCTGGCGC
CGGCCGAAGTCTCGAAGGTCGTGATCGACGAAGATCGCGAGCGCATCGAGGTTGTGGTTCCCGACACCAATAACCAATTA
TCCCTTGCGATCGGCCGTCGCGGCCAGAACGTTCGTTTGGCCTCGCAGCTCACCGGCTGGGACATCGACATCCTGACCGA
GACCGAGGAATCCGAGCGCCGCCAGGCCGATTTCGAGAATTCGACCCGGGTGTTCATGGAAGCGCTGAACGTCGACGAAG
TGGTCGGCCAGCTGCTCGCCTCCGAGGGGTTCACCTCGGTCGAGGAACTGGCGCTGGTCGATATCCGCGAACTGGCGTCG
ATCGAGGGTTTCGACGAGGAAACCGCGACCGAGCTGCAGGCCCGCGCCAGCGAATATCTCGATCGGGTGGAGACGGAAAT
GGAGGCGCGGCGCCTGGAACTCGGCGTCGAGGACGCCCTCAAGGACGTCCCCGGCATCACCTCGAAGATTCTGGTCAAGC
TCGGCGAGGGCGACGTCAAGACGGTCGAGGATCTGGCCGGCTGCGCCACCGACGATCTGGTCGGCTGGACCGAGCGCAAG
GAAGGCGCCGAGCCGGTGAAGTTCGCCGGCATTCTCGACGGCGTCGAGGGCGTCACGCGCGACGAGGCCGAAGACCTGAT
CATGCAGGCCCGCGTCAAGGCCGGCTGGATCACCGAGGAGGAACTCGCCAGCAGCAAGGGCGAGGCCGCCATTGCCGAGA
CCGAAGCCGAGGCGGAGTGA

Upstream 100 bases:

>100_bases
AAGCGGCCGCCAAGCCGAAGCCCACCAATACCAAGCAACATCGTCTCGCCGCCGGCAGGTCGCGGCGTGGCGACACCGAC
CTGTCCGAGGGAGACTGACC

Downstream 100 bases:

>100_bases
CCGTCGTGGCTTCGCGATCCGAACCCATGCGTTTCGAGACATGAAAGGCCGATCGACCGAATGCTCGCTGCCGCCGATGA
TGCCGCAGATCTCGACGACG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MAVSANKLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDAKKGELRLSRHMLVVENVENPANQI
SLKAAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYSEFKDRIGDIVNGVVKRVEYGSVIVDLG
RGEAIVRRDEMLPRESFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKVVIDEDRERIEVVVPDTNNQL
SLAIGRRGQNVRLASQLTGWDIDILTETEESERRQADFENSTRVFMEALNVDEVVGQLLASEGFTSVEELALVDIRELAS
IEGFDEETATELQARASEYLDRVETEMEARRLELGVEDALKDVPGITSKILVKLGEGDVKTVEDLAGCATDDLVGWTERK
EGAEPVKFAGILDGVEGVTRDEAEDLIMQARVKAGWITEEELASSKGEAAIAETEAEAE

Sequences:

>Translated_539_residues
MAVSANKLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDAKKGELRLSRHMLVVENVENPANQI
SLKAAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYSEFKDRIGDIVNGVVKRVEYGSVIVDLG
RGEAIVRRDEMLPRESFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKVVIDEDRERIEVVVPDTNNQL
SLAIGRRGQNVRLASQLTGWDIDILTETEESERRQADFENSTRVFMEALNVDEVVGQLLASEGFTSVEELALVDIRELAS
IEGFDEETATELQARASEYLDRVETEMEARRLELGVEDALKDVPGITSKILVKLGEGDVKTVEDLAGCATDDLVGWTERK
EGAEPVKFAGILDGVEGVTRDEAEDLIMQARVKAGWITEEELASSKGEAAIAETEAEAE
>Mature_538_residues
AVSANKLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDAKKGELRLSRHMLVVENVENPANQIS
LKAAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYSEFKDRIGDIVNGVVKRVEYGSVIVDLGR
GEAIVRRDEMLPRESFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKIG
VVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKVVIDEDRERIEVVVPDTNNQLS
LAIGRRGQNVRLASQLTGWDIDILTETEESERRQADFENSTRVFMEALNVDEVVGQLLASEGFTSVEELALVDIRELASI
EGFDEETATELQARASEYLDRVETEMEARRLELGVEDALKDVPGITSKILVKLGEGDVKTVEDLAGCATDDLVGWTERKE
GAEPVKFAGILDGVEGVTRDEAEDLIMQARVKAGWITEEELASSKGEAAIAETEAEAE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=513, Percent_Identity=42.495126705653, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59172; Mature: 59041

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSANKLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDAKKGE
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCC
LRLSRHMLVVENVENPANQISLKAAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIV
EEHHHCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKVREAERDRQYSEFKDRIGDIVNGVVKRVEYGSVIVDLGRGEAIVRRDEMLPRESFRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHCCCHHHHCCC
DRVRAYIFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
CCEEHHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTETEESERRQADFEN
HCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCCEEEEECCCHHHHHHCCCCC
STRVFMEALNVDEVVGQLLASEGFTSVEELALVDIRELASIEGFDEETATELQARASEYL
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
DRVETEMEARRLELGVEDALKDVPGITSKILVKLGEGDVKTVEDLAGCATDDLVGWTERK
HHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHCCCCHHHCCCHHHC
EGAEPVKFAGILDGVEGVTRDEAEDLIMQARVKAGWITEEELASSKGEAAIAETEAEAE
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCC
>Mature Secondary Structure 
AVSANKLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDAKKGE
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCC
LRLSRHMLVVENVENPANQISLKAAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIV
EEHHHCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKVREAERDRQYSEFKDRIGDIVNGVVKRVEYGSVIVDLGRGEAIVRRDEMLPRESFRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHCCCHHHHCCC
DRVRAYIFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
CCEEHHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTETEESERRQADFEN
HCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCCEEEEECCCHHHHHHCCCCC
STRVFMEALNVDEVVGQLLASEGFTSVEELALVDIRELASIEGFDEETATELQARASEYL
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
DRVETEMEARRLELGVEDALKDVPGITSKILVKLGEGDVKTVEDLAGCATDDLVGWTERK
HHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHCCCCHHHCCCHHHC
EGAEPVKFAGILDGVEGVTRDEAEDLIMQARVKAGWITEEELASSKGEAAIAETEAEAE
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]