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The map label for this gene is caiD [C]

Identifier: 86747683

GI number: 86747683

Start: 627512

End: 628315

Strand: Reverse

Name: caiD [C]

Synonym: RPB_0557

Alternate gene names: 86747683

Gene position: 628315-627512 (Counterclockwise)

Preceding gene: 86747684

Following gene: 86747679

Centisome position: 11.78

GC content: 67.91

Gene sequence:

>804_bases
ATGACCACCAAGGATATTGCCGTCGAAGTCACCGGCCACGTCGCGCTGATCGAGATCCGGCGGCCGCCGCTGAACTTTTT
CGACATCGCGCTGATCCAGCAGATCGCCGATGCGCTCGACGCCATCGACGCCGATCCGGAGATCCGCGCCACCGTGCTGG
CGGCGCAGGGCAAGGCGTTCTGCGCCGGCGCCAATTTCGGCGATCCGGCGCGGCAGGAGGCCGATCCGCAGGCCGCCAAG
GGCGACCCCGCCGACAGCCTCGGCCAGATCGGCCATCTGTACATCGAGGCGGTCAGGATTTTTCGGGCCAAGAAGCCGAT
CGTCGCGGCCATCCACGGCGCCGCGATCGGCGGCGGGCTCGGGCTCGCGGTGTCGGCGGATTTCCGCGTCACCTGCCCGG
AGGCGCGGTTCTCGGCGAATTTCACCAAGCTCGGCTTCCATCCCGGCTTCGGCCTGACGGTGACGCTGCCCGAACTGATC
GGGCGCAACAATGCCGAGCTGATGTTCTACACCTCGCGCCGCGTCACCGGCGAGGACGCGGTGAAGATGGGGCTGGCCAA
CGAGCTGGTGCCGCTGAACGAGGTGCGTAGCGCGGCGCTGAAGCTCGCCCGCGAAATCGCCGAATGCGCGCCGCTGGGCC
TGTTGTCGACCCGCGCGACGATGCGCGCCACGCTGGCCGACCGGGTGATGGAAGCCACCAGGCACGAACTCGCCGAACAG
ACGCGGCTGCGCTCCACCGAAGACTTCAAGGAAGGCGTGAAAGCCACCGAAGAACGCCGCATCGCGCATTTCAAGGGGCG
GTAG

Upstream 100 bases:

>100_bases
ACTCACGATTCAGGGTGGCACCACCGAGGTGCTGCGCGGCATCATCGCGCGCGGACTCGGCCTGCGCTGAGCCGCGCGCC
CAAAGATCAAGGAACACGTC

Downstream 100 bases:

>100_bases
TTACTTCACAGTCCCCAGTCTTGTCATTCCGGGGCGCGCACAGCGCGAACCCGGAATCCATACCCCCGGCCTCGCGGTGA
AATGCGTGCGCCGACGCTCT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTTKDIAVEVTGHVALIEIRRPPLNFFDIALIQQIADALDAIDADPEIRATVLAAQGKAFCAGANFGDPARQEADPQAAK
GDPADSLGQIGHLYIEAVRIFRAKKPIVAAIHGAAIGGGLGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELI
GRNNAELMFYTSRRVTGEDAVKMGLANELVPLNEVRSAALKLAREIAECAPLGLLSTRATMRATLADRVMEATRHELAEQ
TRLRSTEDFKEGVKATEERRIAHFKGR

Sequences:

>Translated_267_residues
MTTKDIAVEVTGHVALIEIRRPPLNFFDIALIQQIADALDAIDADPEIRATVLAAQGKAFCAGANFGDPARQEADPQAAK
GDPADSLGQIGHLYIEAVRIFRAKKPIVAAIHGAAIGGGLGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELI
GRNNAELMFYTSRRVTGEDAVKMGLANELVPLNEVRSAALKLAREIAECAPLGLLSTRATMRATLADRVMEATRHELAEQ
TRLRSTEDFKEGVKATEERRIAHFKGR
>Mature_266_residues
TTKDIAVEVTGHVALIEIRRPPLNFFDIALIQQIADALDAIDADPEIRATVLAAQGKAFCAGANFGDPARQEADPQAAKG
DPADSLGQIGHLYIEAVRIFRAKKPIVAAIHGAAIGGGLGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELIG
RNNAELMFYTSRRVTGEDAVKMGLANELVPLNEVRSAALKLAREIAECAPLGLLSTRATMRATLADRVMEATRHELAEQT
RLRSTEDFKEGVKATEERRIAHFKGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=253, Percent_Identity=29.2490118577075, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=29.5719844357977, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594469, Length=189, Percent_Identity=32.2751322751323, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI37594471, Length=189, Percent_Identity=32.2751322751323, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI68989263, Length=261, Percent_Identity=27.9693486590038, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI25453481, Length=250, Percent_Identity=22.8, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI51538573, Length=250, Percent_Identity=22.8, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI20127408, Length=202, Percent_Identity=31.6831683168317, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4757968, Length=267, Percent_Identity=22.8464419475655, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI49355787, Length=267, Percent_Identity=22.8464419475655, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=222, Percent_Identity=23.4234234234234, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=222, Percent_Identity=23.4234234234234, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136756, Length=250, Percent_Identity=22.4, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=250, Percent_Identity=22.4, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=250, Percent_Identity=22.4, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI62530384, Length=249, Percent_Identity=22.4899598393574, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=30.1526717557252, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=29.4339622641509, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788597, Length=271, Percent_Identity=27.3062730627306, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082183, Length=272, Percent_Identity=25.3676470588235, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=239, Percent_Identity=24.6861924686192, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=30.1980198019802, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=258, Percent_Identity=30.6201550387597, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=271, Percent_Identity=29.1512915129151, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=258, Percent_Identity=24.8062015503876, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=27.8846153846154, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=245, Percent_Identity=26.1224489795918, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=26.4, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=224, Percent_Identity=26.3392857142857, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=193, Percent_Identity=30.5699481865285, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=204, Percent_Identity=28.4313725490196, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=204, Percent_Identity=28.4313725490196, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=187, Percent_Identity=26.7379679144385, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=162, Percent_Identity=31.4814814814815, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=26.2295081967213, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=30.4182509505703, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=30.4182509505703, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=29.8850574712644, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920382, Length=247, Percent_Identity=27.1255060728745, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=27.734375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650670, Length=223, Percent_Identity=29.1479820627803, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28769; Mature: 28638

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKDIAVEVTGHVALIEIRRPPLNFFDIALIQQIADALDAIDADPEIRATVLAAQGKAF
CCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEE
CAGANFGDPARQEADPQAAKGDPADSLGQIGHLYIEAVRIFRAKKPIVAAIHGAAIGGGL
EECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCC
GLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELIGRNNAELMFYTSRRVTGEDA
EEEEECCEEEECCCHHHCCCCEEECCCCCCCEEEECHHHHCCCCCEEEEEECCCCCCHHH
VKMGLANELVPLNEVRSAALKLAREIAECAPLGLLSTRATMRATLADRVMEATRHELAEQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRLRSTEDFKEGVKATEERRIAHFKGR
HHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTKDIAVEVTGHVALIEIRRPPLNFFDIALIQQIADALDAIDADPEIRATVLAAQGKAF
CCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEE
CAGANFGDPARQEADPQAAKGDPADSLGQIGHLYIEAVRIFRAKKPIVAAIHGAAIGGGL
EECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCC
GLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELIGRNNAELMFYTSRRVTGEDA
EEEEECCEEEECCCHHHCCCCEEECCCCCCCEEEECHHHHCCCCCEEEEEECCCCCCHHH
VKMGLANELVPLNEVRSAALKLAREIAECAPLGLLSTRATMRATLADRVMEATRHELAEQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRLRSTEDFKEGVKATEERRIAHFKGR
HHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA