Click here to switch to the map view.

The map label for this gene is vsr [H]

Identifier: 86747597

GI number: 86747597

Start: 519284

End: 519703

Strand: Reverse

Name: vsr [H]

Synonym: RPB_0471

Alternate gene names: 86747597

Gene position: 519703-519284 (Counterclockwise)

Preceding gene: 86747598

Following gene: 86747596

Centisome position: 9.75

GC content: 64.76

Gene sequence:

>420_bases
ATGAGTTCGACCGCCTGCGAGACGCCGGTCGACCCCGCGCGGTCGGCCTTGATGCGCCGGGTTCGGCAGAAGCGCACGAG
GGCGGAGGACGACGTCGCCGACGTGCTGCGTGAACTGGGCTTGCGCTTCCGACGCAACGTGAGAACGCTGCCGGGCTCCC
CCGATTTCGCCAACAAGACCCACCATTGGGCGATATTCGTCAACGGCTGCTTCTGGCATCGTCACCAGGGATGCCACCGA
ACCACCACGCCGACGCGCAATCGGGAGTTCTGGCTGGCGAAGTTCGCGGCCAACGTGAAGCGAGACAAGATCAAGACGCG
CCTTCTGCGTGCGATGGGGTTCAGGGTGGTGACGATCTGGGAATGCGAGACGCGCACGCCGACCAAGGTGCGACGTCGGC
TGAAAGGGCTGATGAGGTAG

Upstream 100 bases:

>100_bases
GCTTCGTACCGGGCTTCAAGGACAGGGTGCGATCTCATCTCGATCATGTTCGAGCCATCGACGCGTTCGTCCAGGAAGGC
CGCACGCTGCTCGCGGCCGA

Downstream 100 bases:

>100_bases
CGGATGGCGAAAGCCAAGACCCCCACAAGACGATTCTCGACGGACAAGACCGCCGTATCGGAAAATTCGATTCGTCGAGA
GCTCGATTGGGCCGGCGATC

Product: putative patch repair protein

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MSSTACETPVDPARSALMRRVRQKRTRAEDDVADVLRELGLRFRRNVRTLPGSPDFANKTHHWAIFVNGCFWHRHQGCHR
TTTPTRNREFWLAKFAANVKRDKIKTRLLRAMGFRVVTIWECETRTPTKVRRRLKGLMR

Sequences:

>Translated_139_residues
MSSTACETPVDPARSALMRRVRQKRTRAEDDVADVLRELGLRFRRNVRTLPGSPDFANKTHHWAIFVNGCFWHRHQGCHR
TTTPTRNREFWLAKFAANVKRDKIKTRLLRAMGFRVVTIWECETRTPTKVRRRLKGLMR
>Mature_138_residues
SSTACETPVDPARSALMRRVRQKRTRAEDDVADVLRELGLRFRRNVRTLPGSPDFANKTHHWAIFVNGCFWHRHQGCHRT
TTPTRNREFWLAKFAANVKRDKIKTRLLRAMGFRVVTIWECETRTPTKVRRRLKGLMR

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=117, Percent_Identity=39.3162393162393, Blast_Score=89, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16415; Mature: 16284

Theoretical pI: Translated: 12.14; Mature: 12.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTACETPVDPARSALMRRVRQKRTRAEDDVADVLRELGLRFRRNVRTLPGSPDFANKT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HHWAIFVNGCFWHRHQGCHRTTTPTRNREFWLAKFAANVKRDKIKTRLLRAMGFRVVTIW
CEEEEEEECCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
ECETRTPTKVRRRLKGLMR
ECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SSTACETPVDPARSALMRRVRQKRTRAEDDVADVLRELGLRFRRNVRTLPGSPDFANKT
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HHWAIFVNGCFWHRHQGCHRTTTPTRNREFWLAKFAANVKRDKIKTRLLRAMGFRVVTIW
CEEEEEEECCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
ECETRTPTKVRRRLKGLMR
ECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]