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The map label for this gene is ydiP [H]

Identifier: 86747598

GI number: 86747598

Start: 519700

End: 520872

Strand: Reverse

Name: ydiP [H]

Synonym: RPB_0472

Alternate gene names: 86747598

Gene position: 520872-519700 (Counterclockwise)

Preceding gene: 86747601

Following gene: 86747597

Centisome position: 9.77

GC content: 65.73

Gene sequence:

>1173_bases
ATGAAAATTGCAGGACTTTTCGCTGGCATCGGAGGGCTCGAGCTTGGTCTTCATCGGGCCGGGCACGAGACGGTAATCCT
GTCCGAAATCTGGCAGCCCGCCGGGGCGGTGTTGGAACACCGCTTCAAGGGCGCCCCGAATGTCGGCGATGTCGCGACGT
TGACGTCGCTGCCCTCCGAGGTCGAGCTGATGACGGCCGGCTTTCCCTGCCAGGACCTCAGCCAAGCCGGCAAGACCGCC
GGGATCAAGGGCGGGAAGTCGGGGCTGGTGACGCATGTTTTCCGGCTGATCGATCGCAGCCGGCCGAAATGGGTGCTGCT
GGAGAACGTCTCGTTCATGCTGCGCCTCGACGGCGGCAGCGCCATGACCCGGCTGGTCTCCGAATTCGAAAGGCGCGGCT
ATCGCTGGGCCTACCGCGTTGTGAATTCACTGAGCTTCTTGCCGCATCGGCGCGCGCGGGTGTTCTTTCTGGCGAGCATC
GAGGGTGATCCGGCCGATGTCCTGCTGGTCGACGACGCGGAGCCCGCCGGGCTGCAGACAAGCCTGCAAAGCCACGCCCA
CGGCTTCTACTGGACCGAAGGAACGCGCGGTCTGGGATGGGGCCCCGACTGCGTGCCGACGCTCAAGAACGGCTCCACGG
TCGGCATTCCTTCGCCGCCGGCGATCCTGATGCCGAACGGGGAAATCGTGACACCCGATATCCGCGACGCCGAGCGGCTT
CAGGGACTTCCCGCCGACTGGACGAAGCCGGCCGAGAGGGTGGCCCGCGCGTCGTTCCGATGGTCGCTGGTCGGCAATGC
CGTCAGCAAGCCGGTCGCGGCGTGGATCGGACAGCGGTTGAACGCGCCCGGAGCATACGACAGATCGCGCGACGGCGGCA
GCGTCGTCCGCGGCGATTGGCCCAAGGCGGCGCGCTCCGACGGCAAAGCCTGCCGTGAAGTCGCGATCTCCGAATTTCCC
AAATGGGCGAAGCGGCCGAGCCTTCAGGACTTCCTGCGCTACGAGCCGAGACTGCTGTCGGCTCGCGCCACGGCAGGGTT
TTTGTCGCGGATCGAGAAGAGCAGCTTGCGCTTCGTACCGGGCTTCAAGGACAGGGTGCGATCTCATCTCGATCATGTTC
GAGCCATCGACGCGTTCGTCCAGGAAGGCCGCACGCTGCTCGCGGCCGAATGA

Upstream 100 bases:

>100_bases
CTTGCGGCCCGACCGATGGAGCGCGTACGAGCTCGCATCGGGCCTTCAGTTCGGCCTTGCCAAATACGAACAATTAGGGA
ACATTGCCGGCGGGGGCATC

Downstream 100 bases:

>100_bases
GTTCGACCGCCTGCGAGACGCCGGTCGACCCCGCGCGGTCGGCCTTGATGCGCCGGGTTCGGCAGAAGCGCACGAGGGCG
GAGGACGACGTCGCCGACGT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M2.BsuMI; Cytosine-specific methyltransferase M2.BsuMI [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKIAGLFAGIGGLELGLHRAGHETVILSEIWQPAGAVLEHRFKGAPNVGDVATLTSLPSEVELMTAGFPCQDLSQAGKTA
GIKGGKSGLVTHVFRLIDRSRPKWVLLENVSFMLRLDGGSAMTRLVSEFERRGYRWAYRVVNSLSFLPHRRARVFFLASI
EGDPADVLLVDDAEPAGLQTSLQSHAHGFYWTEGTRGLGWGPDCVPTLKNGSTVGIPSPPAILMPNGEIVTPDIRDAERL
QGLPADWTKPAERVARASFRWSLVGNAVSKPVAAWIGQRLNAPGAYDRSRDGGSVVRGDWPKAARSDGKACREVAISEFP
KWAKRPSLQDFLRYEPRLLSARATAGFLSRIEKSSLRFVPGFKDRVRSHLDHVRAIDAFVQEGRTLLAAE

Sequences:

>Translated_390_residues
MKIAGLFAGIGGLELGLHRAGHETVILSEIWQPAGAVLEHRFKGAPNVGDVATLTSLPSEVELMTAGFPCQDLSQAGKTA
GIKGGKSGLVTHVFRLIDRSRPKWVLLENVSFMLRLDGGSAMTRLVSEFERRGYRWAYRVVNSLSFLPHRRARVFFLASI
EGDPADVLLVDDAEPAGLQTSLQSHAHGFYWTEGTRGLGWGPDCVPTLKNGSTVGIPSPPAILMPNGEIVTPDIRDAERL
QGLPADWTKPAERVARASFRWSLVGNAVSKPVAAWIGQRLNAPGAYDRSRDGGSVVRGDWPKAARSDGKACREVAISEFP
KWAKRPSLQDFLRYEPRLLSARATAGFLSRIEKSSLRFVPGFKDRVRSHLDHVRAIDAFVQEGRTLLAAE
>Mature_390_residues
MKIAGLFAGIGGLELGLHRAGHETVILSEIWQPAGAVLEHRFKGAPNVGDVATLTSLPSEVELMTAGFPCQDLSQAGKTA
GIKGGKSGLVTHVFRLIDRSRPKWVLLENVSFMLRLDGGSAMTRLVSEFERRGYRWAYRVVNSLSFLPHRRARVFFLASI
EGDPADVLLVDDAEPAGLQTSLQSHAHGFYWTEGTRGLGWGPDCVPTLKNGSTVGIPSPPAILMPNGEIVTPDIRDAERL
QGLPADWTKPAERVARASFRWSLVGNAVSKPVAAWIGQRLNAPGAYDRSRDGGSVVRGDWPKAARSDGKACREVAISEFP
KWAKRPSLQDFLRYEPRLLSARATAGFLSRIEKSSLRFVPGFKDRVRSHLDHVRAIDAFVQEGRTLLAAE

Specific function: This methylase may recognize the double-stranded sequence CTCGAG, causing specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsuMI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 42620; Mature: 42620

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAGLFAGIGGLELGLHRAGHETVILSEIWQPAGAVLEHRFKGAPNVGDVATLTSLPSE
CCCHHHHHCCCHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHHCCCCCCCCHHHHHCCCCC
VELMTAGFPCQDLSQAGKTAGIKGGKSGLVTHVFRLIDRSRPKWVLLENVSFMLRLDGGS
CEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCH
AMTRLVSEFERRGYRWAYRVVNSLSFLPHRRARVFFLASIEGDPADVLLVDDAEPAGLQT
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHH
SLQSHAHGFYWTEGTRGLGWGPDCVPTLKNGSTVGIPSPPAILMPNGEIVTPDIRDAERL
HHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEEECCCCCEECCCCCCHHHH
QGLPADWTKPAERVARASFRWSLVGNAVSKPVAAWIGQRLNAPGAYDRSRDGGSVVRGDW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
PKAARSDGKACREVAISEFPKWAKRPSLQDFLRYEPRLLSARATAGFLSRIEKSSLRFVP
CCHHHCCHHHHHHHHHHHCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
GFKDRVRSHLDHVRAIDAFVQEGRTLLAAE
CHHHHHHHHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MKIAGLFAGIGGLELGLHRAGHETVILSEIWQPAGAVLEHRFKGAPNVGDVATLTSLPSE
CCCHHHHHCCCHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHHCCCCCCCCHHHHHCCCCC
VELMTAGFPCQDLSQAGKTAGIKGGKSGLVTHVFRLIDRSRPKWVLLENVSFMLRLDGGS
CEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCH
AMTRLVSEFERRGYRWAYRVVNSLSFLPHRRARVFFLASIEGDPADVLLVDDAEPAGLQT
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHH
SLQSHAHGFYWTEGTRGLGWGPDCVPTLKNGSTVGIPSPPAILMPNGEIVTPDIRDAERL
HHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEEECCCCCEECCCCCCHHHH
QGLPADWTKPAERVARASFRWSLVGNAVSKPVAAWIGQRLNAPGAYDRSRDGGSVVRGDW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
PKAARSDGKACREVAISEFPKWAKRPSLQDFLRYEPRLLSARATAGFLSRIEKSSLRFVP
CCHHHCCHHHHHHHHHHHCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
GFKDRVRSHLDHVRAIDAFVQEGRTLLAAE
CHHHHHHHHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]