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The map label for this gene is lepA [H]

Identifier: 86747595

GI number: 86747595

Start: 516521

End: 518455

Strand: Direct

Name: lepA [H]

Synonym: RPB_0469

Alternate gene names: 86747595

Gene position: 516521-518455 (Clockwise)

Preceding gene: 86747593

Following gene: 86747599

Centisome position: 9.69

GC content: 63.62

Gene sequence:

>1935_bases
ATGCGCGGCGGACATCAGCCGGTTCGATCGTCCCCGTCAAGCACGGCAAAAGCGGCGGTTTTTCGGGCGGTTGCGGGCGC
CCTTCGCCTTGCCGCGCCGGAGTCCCGGTGCTATCTCGCGACCATGACCACCGCCCCCATCGACAACATCCGCAACTTCT
CCATCGTCGCCCATATCGACCATGGCAAGTCGACGCTCGCCGACCGGCTGATCCAGATCACCGGCGGGATGTCCGACCGC
GAAATGGCCGGCAAGGAGCAGGTGCTCGATTCGATGGATATCGAGCGCGAGCGCGGCATCACCATCAAGGCGCAGACCGT
GCGCCTGAACTACCGCGCCAAGGACGGCAAGGACTACATCTTCAACCTGATGGACACGCCCGGCCACGTCGACTTCGCCT
ACGAAGTGTCGCGGTCGCTGGCGGCCTGCGAGGGTTCGCTGCTGGTGGTCGACGCCTCGCAGGGCGTCGAGGCGCAAACG
CTGGCCAATGTCTATCACGCGCTCGACGCCGGCCACGAGATCGTGCCGGTGCTCAACAAGGTCGATCTGCCGGCCGCCGA
GCCCGACAAGGTCAAGCAGCAGATCGAGGACGTGATCGGGCTCGATGCCTCCGACGCGGTGATGATCTCGGCAAAGACCG
GCCTCGGCGTGCCCGACGTGCTGGAGGCGATCGTGACCCGGCTGCCGCCGCCGCAAGGCGACCGCTCCGCGCAGCTCAAG
GCGCTGCTGGTCGATAGCTGGTACGACGTCTATCTCGGCGTCGTGGTGCTGGTCCGCGTCGTCGACGGCGTGATGAAGAA
GGGCCAGCGCATCCGGATGATGGGCACCAACGCCGCCTATGATCTGGAACGCGTCGGCTACTTCACCCCGAAGATGACCT
CGGTCGACGAACTCGGCCCCGGCGAGATCGGCTTCATCACCGCGGCGATCAAGGAAGTCGCCGACACGAGAGTCGGCGAC
ACGATTACGGACGACAGGAAACCGATCACCGAGATGCTGCCGGGCTTCAAGCCGGCGATCCCGGTGGTGTTCTGCGGCCT
GTTCCCGGTCGACGCCGACGATTTCGAGACGCTGCGCGGCGCGATGGGCAAACTCAGGCTCAACGACGCCTCGTTCTCGT
TCGAAATGGAAACCTCCGCCGCGCTCGGCTTCGGCTTCCGCTGCGGCTTCCTGGGGTTGCTGCACCTGGAGATCATCCAG
GAGCGACTGTCCCGCGAATTCGACCTCAATTTGATCGCGACCGCGCCGAGCGTCATCTACAAGATGACGCTGACGGACGG
CACCGAAATGGAGATCCACAATCCGGTCGACATGCCGGACGTGGTCAAGATCGCCGAAATCCAGGAGCCGTGGATCGAAG
CGACGATTCTGACGCCCGACGAATATCTCGGCTCGGTGCTGAAGCTGTGCCAGGACCGCCGCGGCAATCAGAAGGAGCTG
ACTTACGTCGGCGCCCGCGCGATGGTGAAATACGACCTGCCGCTCAACGAAGTGGTGTTCGATTTCTACGACCGCCTCAA
GAGCGTGTCGAAGGGCTACGCCTCGTTCGACTATCACCTCACCGAATACAAGCCGGCCGATCTGGTGAAGATGCAGATCC
TCGTCAACGCCGAGCCGGTCGACGCGCTGTCGATGCTGGTGCACAGGACGCGCGCCGAAGGCCGCGGCCGCGCCATGGTG
GAGAAGATGAAGGAGCTGATCCCGCCGCACATGTTCGTCATCCCGATCCAGGCGGCGATCGGCGGCAAGATCATCGCCCG
CGAAACCGTCCGCGCCCTGCGCAAGGACGTCACCGCCAAATGCTACGGCGGCGACATCACCCGCAAACGCAAACTTCTGG
AGAAACAGAAGGAGGGCAAAAAGAAGATGCGCCAGTTCGGCAAGGTGGATATTCCGCAAGAGGCGTTCATTGCGGCGCTG
AAGGTGGATAGTTGA

Upstream 100 bases:

>100_bases
TGCCGTTTGGGATCGAGCAGGGCGTCGATCCAGCGCGCGATCAGCGACAGGCGGGCGCGGGACGTGGCGATCGAGGTCAT
CCGGACCCTGGGTTCGAGGC

Downstream 100 bases:

>100_bases
GGGTCAGACCTCGTCGTCGTCATCTTCGCGCCCGGGCGCAGCAAGAATCTCAGTGTCGCCCTCGGCCCATTTGAAGGCTG
ATTTGTTCCGCGCGTCGTAG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MRGGHQPVRSSPSSTAKAAVFRAVAGALRLAAPESRCYLATMTTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDR
EMAGKEQVLDSMDIERERGITIKAQTVRLNYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQT
LANVYHALDAGHEIVPVLNKVDLPAAEPDKVKQQIEDVIGLDASDAVMISAKTGLGVPDVLEAIVTRLPPPQGDRSAQLK
ALLVDSWYDVYLGVVVLVRVVDGVMKKGQRIRMMGTNAAYDLERVGYFTPKMTSVDELGPGEIGFITAAIKEVADTRVGD
TITDDRKPITEMLPGFKPAIPVVFCGLFPVDADDFETLRGAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQ
ERLSREFDLNLIATAPSVIYKMTLTDGTEMEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKEL
TYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLTEYKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMV
EKMKELIPPHMFVIPIQAAIGGKIIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAAL
KVDS

Sequences:

>Translated_644_residues
MRGGHQPVRSSPSSTAKAAVFRAVAGALRLAAPESRCYLATMTTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDR
EMAGKEQVLDSMDIERERGITIKAQTVRLNYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQT
LANVYHALDAGHEIVPVLNKVDLPAAEPDKVKQQIEDVIGLDASDAVMISAKTGLGVPDVLEAIVTRLPPPQGDRSAQLK
ALLVDSWYDVYLGVVVLVRVVDGVMKKGQRIRMMGTNAAYDLERVGYFTPKMTSVDELGPGEIGFITAAIKEVADTRVGD
TITDDRKPITEMLPGFKPAIPVVFCGLFPVDADDFETLRGAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQ
ERLSREFDLNLIATAPSVIYKMTLTDGTEMEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKEL
TYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLTEYKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMV
EKMKELIPPHMFVIPIQAAIGGKIIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAAL
KVDS
>Mature_644_residues
MRGGHQPVRSSPSSTAKAAVFRAVAGALRLAAPESRCYLATMTTAPIDNIRNFSIVAHIDHGKSTLADRLIQITGGMSDR
EMAGKEQVLDSMDIERERGITIKAQTVRLNYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQT
LANVYHALDAGHEIVPVLNKVDLPAAEPDKVKQQIEDVIGLDASDAVMISAKTGLGVPDVLEAIVTRLPPPQGDRSAQLK
ALLVDSWYDVYLGVVVLVRVVDGVMKKGQRIRMMGTNAAYDLERVGYFTPKMTSVDELGPGEIGFITAAIKEVADTRVGD
TITDDRKPITEMLPGFKPAIPVVFCGLFPVDADDFETLRGAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQ
ERLSREFDLNLIATAPSVIYKMTLTDGTEMEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKEL
TYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLTEYKPADLVKMQILVNAEPVDALSMLVHRTRAEGRGRAMV
EKMKELIPPHMFVIPIQAAIGGKIIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAAL
KVDS

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=634, Percent_Identity=48.4227129337539, Blast_Score=622, Evalue=1e-178,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=47.0149253731343, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI18390331, Length=186, Percent_Identity=34.4086021505376, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=40, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=45.945945945946, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=45.945945945946, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=45.945945945946, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI25306283, Length=147, Percent_Identity=44.8979591836735, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI19923640, Length=147, Percent_Identity=44.8979591836735, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI25306287, Length=147, Percent_Identity=44.8979591836735, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI53729339, Length=222, Percent_Identity=27.9279279279279, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI53729337, Length=222, Percent_Identity=27.9279279279279, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=58.5858585858586, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=521, Percent_Identity=28.4069097888676, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789738, Length=162, Percent_Identity=36.4197530864198, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1790835, Length=168, Percent_Identity=31.547619047619, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789559, Length=224, Percent_Identity=29.4642857142857, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=624, Percent_Identity=38.6217948717949, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71988819, Length=139, Percent_Identity=38.8489208633094, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71988811, Length=139, Percent_Identity=38.8489208633094, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17556745, Length=171, Percent_Identity=34.5029239766082, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=180, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=159, Percent_Identity=37.7358490566038, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=33.1034482758621, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=225, Percent_Identity=27.5555555555556, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=611, Percent_Identity=46.9721767594108, Blast_Score=558, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=41.4965986394558, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=41.4965986394558, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=35.5191256830601, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324166, Length=171, Percent_Identity=36.2573099415205, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322359, Length=125, Percent_Identity=37.6, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=285, Percent_Identity=27.0175438596491, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706572, Length=605, Percent_Identity=45.2892561983471, Blast_Score=558, Evalue=1e-159,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=44.9275362318841, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=38.0368098159509, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=38.4105960264901, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=38.4105960264901, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=38.4105960264901, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357743, Length=168, Percent_Identity=34.5238095238095, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=39.0728476821192, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363316, Length=246, Percent_Identity=26.4227642276423, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17864358, Length=246, Percent_Identity=26.4227642276423, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 70995; Mature: 70995

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGGHQPVRSSPSSTAKAAVFRAVAGALRLAAPESRCYLATMTTAPIDNIRNFSIVAHID
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEC
HGKSTLADRLIQITGGMSDREMAGKEQVLDSMDIERERGITIKAQTVRLNYRAKDGKDYI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCHHHCCCEEEEEEEEEEEECCCCCCEE
FNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNK
EEECCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHC
VDLPAAEPDKVKQQIEDVIGLDASDAVMISAKTGLGVPDVLEAIVTRLPPPQGDRSAQLK
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHEE
ALLVDSWYDVYLGVVVLVRVVDGVMKKGQRIRMMGTNAAYDLERVGYFTPKMTSVDELGP
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCC
GEIGFITAAIKEVADTRVGDTITDDRKPITEMLPGFKPAIPVVFCGLFPVDADDFETLRG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHH
AMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLNLIATAPSVIY
HHCCEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEE
KMTLTDGTEMEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKEL
EEEECCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCE
TYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLTEYKPADLVKMQILVNAEPV
EEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHEEEEEEECCCCH
DALSMLVHRTRAEGRGRAMVEKMKELIPPHMFVIPIQAAIGGKIIARETVRALRKDVTAK
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCHHHHHHHHHHHHCCCCE
CYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECC
>Mature Secondary Structure
MRGGHQPVRSSPSSTAKAAVFRAVAGALRLAAPESRCYLATMTTAPIDNIRNFSIVAHID
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEC
HGKSTLADRLIQITGGMSDREMAGKEQVLDSMDIERERGITIKAQTVRLNYRAKDGKDYI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCHHHCCCEEEEEEEEEEEECCCCCCEE
FNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNK
EEECCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHC
VDLPAAEPDKVKQQIEDVIGLDASDAVMISAKTGLGVPDVLEAIVTRLPPPQGDRSAQLK
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHEE
ALLVDSWYDVYLGVVVLVRVVDGVMKKGQRIRMMGTNAAYDLERVGYFTPKMTSVDELGP
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCC
GEIGFITAAIKEVADTRVGDTITDDRKPITEMLPGFKPAIPVVFCGLFPVDADDFETLRG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHH
AMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLNLIATAPSVIY
HHCCEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEE
KMTLTDGTEMEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKEL
EEEECCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCE
TYVGARAMVKYDLPLNEVVFDFYDRLKSVSKGYASFDYHLTEYKPADLVKMQILVNAEPV
EEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHEEEEEEECCCCH
DALSMLVHRTRAEGRGRAMVEKMKELIPPHMFVIPIQAAIGGKIIARETVRALRKDVTAK
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCHHHHHHHHHHHHCCCCE
CYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA