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The map label for this gene is aceK

Identifier: 86747599

GI number: 86747599

Start: 521031

End: 522848

Strand: Direct

Name: aceK

Synonym: RPB_0473

Alternate gene names: 86747599

Gene position: 521031-522848 (Clockwise)

Preceding gene: 86747595

Following gene: 86747600

Centisome position: 9.77

GC content: 68.26

Gene sequence:

>1818_bases
ATGACTGCCACCCGTCATCCCTTTGCCTCCGACATCGACGCCGCCACCCGGATCGCCGAGCCGGATTTCGATCTGCTCGA
CGCGCTGGTGCGGACCGACGATCCGGACGACCAGGCCCACACGCTGGCGCGCGTGGTGTTGGCGGCGTTCGACAATTATT
ACGCGGTGTCGCGGCGGATTCCGGCGCTGGCGCAGGCGGCGTTCGAGGCGCGCGACTGGGCCGCCACGGTGCGGCTGTCG
AAAATCCGGCTCGGGCTGTACACCGCCTGCATCGATCAGCTGGTGCCGCTGCTGAAGGCCGGGCTGCCCGAGCTCGCCAA
TGACGAACAGCTCTGGGCGCGCGCCGAGGCCGAGCTGCTGGCGGCGATCGCCGAGCGCTACGAGGCCGATTTCGCCTTCG
CGTTCTGGCAGTCGCTGCGCCGCAAGCTGGTCAGCGACGAATGGCGGCCGGTGTCCTACGATACCGGGCCGGCGGCGCGG
CCGAAGGCGACCAGCGCTGCGATCCTCAAGACCATCGCCACGACGCTGCCGATCAGGCCGGCGGTGATCCGCGACATCCT
CGACGGCGCCGGCCTCCGCGGGCCGTGGCGCGATCGCGACGGCGACGCCGCGCTCGCCGCGGCCGCGATCGAGGCTGCGC
TGGAGCCGCTCGGCCCGCGCGCCGGCGAGACCGCGAAGATCGAGATCGCCGAGTCCGGCTTCTTCCGCAATCGCGGCGCC
TGCCTGGTCGGGCGCGTCCGGCTGCGCGACCGCGGCGACATGCCGATGCGCAACCTGCCGCTGTTGATCGCGCTGCTCAA
CGAGGACGACGGCCTCGTCGTCGATGCGGTGCTGTGCGACGCCGACGAGCTGCAATACGCGTTCTCCTCGACGCTGGCCA
ATTACCACGCCACCAATCCGCATTATCACGAGCTGGCGCGGCTGTTGCACGAACTGATGCCGAAGCGGCCGCTCGGCACG
CAATATTCCTGCATCGGCTTCCACCACCTCGGCAAGGTCGCGGTGATGACCGAGATTCTCGCCGAGCACCGCAGGAGCAA
GGAGAAGCTCGACACCGCGCCGGGTTTCAAGGGCACGGTGGCGATCGCGTTCACGATGCCGTCCTCGGCCTATGTGCTGA
AGATCATCCGCGACCATCCCACCGACGACTACAAGTTCGACTATTTCGACGGCCTCGACGCGGTGCTGCGCAAATACAAT
CTGGTGCACGAGATCGATCGCGCCGGCTCGATGCTCGACAACATCATCTACTCCAACGTCAAGCTCGAGCGCACGATGTT
CGCGCCCGATCTGCTCGACGAGCTGCTGGAATCCGGCATCGACACCGTGACGCTGGAACGCGGCGCGCTGGTGTTCCGGC
ATCTGATCGTGCAGATCAAGCTGACGCCGCTGCCGCTCTATCTCACCACCGCGTCCGCCGCCGATGCCCGCGCCGCGGTG
ATCAATCTCGGCGACTGCATCAAGAACAACGCCGCCGCCGATATCTTCAACAAGGACCTCGACGGCCGCAACTACGGCGT
CAGCCGCATCCGCAAAGTGTATCTGTTCGACTACGACGCGGTCGAGCCCCTGACCGACGTCCGGGTCCGCGGCGACGACG
CGCCGCCGGGGCCGGAGTTCGAGGACGGCGTGGTGTTCCGCCCGGCCGACATGCTCGGCGGGTTGCGGATCGACGACCCG
GCGCTGCGCCGCGCCTTCCGCGACGCCCATCCCGAGCTGATGCAGCCGGAGTATTGGCAGGGCATGCAGCGCGCGCTGCG
CGCCGGCAAGGTTCCCCGCGTGATGAACTATCCGGCGGCCCGGCGGCTGCGGCGTTAG

Upstream 100 bases:

>100_bases
CCCGATGCGAGCTCGTACGCGCTCCATCGGTCGGGCCGCAAGGCGCCGTTTTGCGTCAGATCACGCCGCCGCGCCCGGAT
CCGTCGCATGATTGCGCCCC

Downstream 100 bases:

>100_bases
CTGCGCGTCTGCCCTCACGCTGAAGCACTGCCGCGCCCGCCGCGGCGACCGGGCTCCGCCCTTGCCGAACCCTCCCGCCG
CCTCTACCTGTGCGAAGCCC

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MTATRHPFASDIDAATRIAEPDFDLLDALVRTDDPDDQAHTLARVVLAAFDNYYAVSRRIPALAQAAFEARDWAATVRLS
KIRLGLYTACIDQLVPLLKAGLPELANDEQLWARAEAELLAAIAERYEADFAFAFWQSLRRKLVSDEWRPVSYDTGPAAR
PKATSAAILKTIATTLPIRPAVIRDILDGAGLRGPWRDRDGDAALAAAAIEAALEPLGPRAGETAKIEIAESGFFRNRGA
CLVGRVRLRDRGDMPMRNLPLLIALLNEDDGLVVDAVLCDADELQYAFSSTLANYHATNPHYHELARLLHELMPKRPLGT
QYSCIGFHHLGKVAVMTEILAEHRRSKEKLDTAPGFKGTVAIAFTMPSSAYVLKIIRDHPTDDYKFDYFDGLDAVLRKYN
LVHEIDRAGSMLDNIIYSNVKLERTMFAPDLLDELLESGIDTVTLERGALVFRHLIVQIKLTPLPLYLTTASAADARAAV
INLGDCIKNNAAADIFNKDLDGRNYGVSRIRKVYLFDYDAVEPLTDVRVRGDDAPPGPEFEDGVVFRPADMLGGLRIDDP
ALRRAFRDAHPELMQPEYWQGMQRALRAGKVPRVMNYPAARRLRR

Sequences:

>Translated_605_residues
MTATRHPFASDIDAATRIAEPDFDLLDALVRTDDPDDQAHTLARVVLAAFDNYYAVSRRIPALAQAAFEARDWAATVRLS
KIRLGLYTACIDQLVPLLKAGLPELANDEQLWARAEAELLAAIAERYEADFAFAFWQSLRRKLVSDEWRPVSYDTGPAAR
PKATSAAILKTIATTLPIRPAVIRDILDGAGLRGPWRDRDGDAALAAAAIEAALEPLGPRAGETAKIEIAESGFFRNRGA
CLVGRVRLRDRGDMPMRNLPLLIALLNEDDGLVVDAVLCDADELQYAFSSTLANYHATNPHYHELARLLHELMPKRPLGT
QYSCIGFHHLGKVAVMTEILAEHRRSKEKLDTAPGFKGTVAIAFTMPSSAYVLKIIRDHPTDDYKFDYFDGLDAVLRKYN
LVHEIDRAGSMLDNIIYSNVKLERTMFAPDLLDELLESGIDTVTLERGALVFRHLIVQIKLTPLPLYLTTASAADARAAV
INLGDCIKNNAAADIFNKDLDGRNYGVSRIRKVYLFDYDAVEPLTDVRVRGDDAPPGPEFEDGVVFRPADMLGGLRIDDP
ALRRAFRDAHPELMQPEYWQGMQRALRAGKVPRVMNYPAARRLRR
>Mature_604_residues
TATRHPFASDIDAATRIAEPDFDLLDALVRTDDPDDQAHTLARVVLAAFDNYYAVSRRIPALAQAAFEARDWAATVRLSK
IRLGLYTACIDQLVPLLKAGLPELANDEQLWARAEAELLAAIAERYEADFAFAFWQSLRRKLVSDEWRPVSYDTGPAARP
KATSAAILKTIATTLPIRPAVIRDILDGAGLRGPWRDRDGDAALAAAAIEAALEPLGPRAGETAKIEIAESGFFRNRGAC
LVGRVRLRDRGDMPMRNLPLLIALLNEDDGLVVDAVLCDADELQYAFSSTLANYHATNPHYHELARLLHELMPKRPLGTQ
YSCIGFHHLGKVAVMTEILAEHRRSKEKLDTAPGFKGTVAIAFTMPSSAYVLKIIRDHPTDDYKFDYFDGLDAVLRKYNL
VHEIDRAGSMLDNIIYSNVKLERTMFAPDLLDELLESGIDTVTLERGALVFRHLIVQIKLTPLPLYLTTASAADARAAVI
NLGDCIKNNAAADIFNKDLDGRNYGVSRIRKVYLFDYDAVEPLTDVRVRGDDAPPGPEFEDGVVFRPADMLGGLRIDDPA
LRRAFRDAHPELMQPEYWQGMQRALRAGKVPRVMNYPAARRLRR

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=579, Percent_Identity=26.5975820379965, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_RHOP2 (Q2J2X6)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484095.1
- STRING:   Q2J2X6
- GeneID:   3909818
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0473
- eggNOG:   COG4579
- HOGENOM:   HBG660857
- OMA:   RMTPLNI
- ProtClustDB:   PRK02946
- BioCyc:   RPAL316058:RPB_0473-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 67294; Mature: 67162

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: ACT_SITE 413-413 BINDING 374-374

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRHPFASDIDAATRIAEPDFDLLDALVRTDDPDDQAHTLARVVLAAFDNYYAVSRRI
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PALAQAAFEARDWAATVRLSKIRLGLYTACIDQLVPLLKAGLPELANDEQLWARAEAELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AAIAERYEADFAFAFWQSLRRKLVSDEWRPVSYDTGPAARPKATSAAILKTIATTLPIRP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCH
AVIRDILDGAGLRGPWRDRDGDAALAAAAIEAALEPLGPRAGETAKIEIAESGFFRNRGA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
CLVGRVRLRDRGDMPMRNLPLLIALLNEDDGLVVDAVLCDADELQYAFSSTLANYHATNP
EEEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCC
HYHELARLLHELMPKRPLGTQYSCIGFHHLGKVAVMTEILAEHRRSKEKLDTAPGFKGTV
CHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
AIAFTMPSSAYVLKIIRDHPTDDYKFDYFDGLDAVLRKYNLVHEIDRAGSMLDNIIYSNV
EEEEECCCCCEEHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLERTMFAPDLLDELLESGIDTVTLERGALVFRHLIVQIKLTPLPLYLTTASAADARAAV
EEHHHHCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHE
INLGDCIKNNAAADIFNKDLDGRNYGVSRIRKVYLFDYDAVEPLTDVRVRGDDAPPGPEF
EEHHHHHCCCCHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCCCHHCEEECCCCCCCCCC
EDGVVFRPADMLGGLRIDDPALRRAFRDAHPELMQPEYWQGMQRALRAGKVPRVMNYPAA
CCCEEECCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCHH
RRLRR
HHHCC
>Mature Secondary Structure 
TATRHPFASDIDAATRIAEPDFDLLDALVRTDDPDDQAHTLARVVLAAFDNYYAVSRRI
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PALAQAAFEARDWAATVRLSKIRLGLYTACIDQLVPLLKAGLPELANDEQLWARAEAELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AAIAERYEADFAFAFWQSLRRKLVSDEWRPVSYDTGPAARPKATSAAILKTIATTLPIRP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCH
AVIRDILDGAGLRGPWRDRDGDAALAAAAIEAALEPLGPRAGETAKIEIAESGFFRNRGA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
CLVGRVRLRDRGDMPMRNLPLLIALLNEDDGLVVDAVLCDADELQYAFSSTLANYHATNP
EEEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCC
HYHELARLLHELMPKRPLGTQYSCIGFHHLGKVAVMTEILAEHRRSKEKLDTAPGFKGTV
CHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
AIAFTMPSSAYVLKIIRDHPTDDYKFDYFDGLDAVLRKYNLVHEIDRAGSMLDNIIYSNV
EEEEECCCCCEEHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLERTMFAPDLLDELLESGIDTVTLERGALVFRHLIVQIKLTPLPLYLTTASAADARAAV
EEHHHHCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHE
INLGDCIKNNAAADIFNKDLDGRNYGVSRIRKVYLFDYDAVEPLTDVRVRGDDAPPGPEF
EEHHHHHCCCCHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCCCHHCEEECCCCCCCCCC
EDGVVFRPADMLGGLRIDDPALRRAFRDAHPELMQPEYWQGMQRALRAGKVPRVMNYPAA
CCCEEECCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCHH
RRLRR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA