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The map label for this gene is pckA

Identifier: 86747587

GI number: 86747587

Start: 508024

End: 509637

Strand: Reverse

Name: pckA

Synonym: RPB_0461

Alternate gene names: 86747587

Gene position: 509637-508024 (Counterclockwise)

Preceding gene: 86747588

Following gene: 86747583

Centisome position: 9.56

GC content: 65.49

Gene sequence:

>1614_bases
GTGCAAGAGACGGGCGTGCATAACGGTGCTCATGGCGCCGACAAATTCGGACTCAAAAATCTCAAGGGCGTCTACTGGAA
TTTCGCCGCGCCGCAGCTCTACGAGCATGCGCTGAAGAACGGCGAAGCGGTGCTGTCGGCCGACGGCGCGCTGTGCGCCG
ACACCGGCGAATTCACCGGCCGCAGCCCGAAGGACAAGTTCACGGTCCGCGACGCCACCACCGAGACCACGATGTGGTGG
GGTGGCAATCAGTCGATCACCGCCGAGCAGTTCGAGACGCTGTATCAGGACTTCCTCAAGCACGCCGAAGGCATGACGCT
GTTCGCGCAGGATCTGTACGGCGGCGCCGATCCGTCGTTCCAGATCAAGACCCGCGTGTTCACCGAACTCGCCTGGCACT
CGCTGTTCATCCGCACCCTGCTGCGCCGTCCCGACCGCGCCGCGCTGGAGAGCTTCGTTCCCGAGCTGACGCTGATCGAT
CTGCCGAGCTTCCGCGCCGATCCGAAGCGCCACGGCTGCCGCTCCGAGAACGTCGTGGCGATCGATTTCGCCCGCAAGAT
CGTGCTGATCGGTGGCACCCAATACGCCGGCGAAATGAAGAAGAGCGTGTTCACCACGCTGAACTACTATCTGCCCGAGA
AGGGCGTGATGCCGATGCACTGCTCGGCCAATGTCGGCCCGCAGGGCGACACCGCGATCTTCTTCGGCCTGTCCGGCACC
GGCAAGACCACGCTGTCGGCCGACCCGAACCGCACGCTGATCGGCGACGACGAGCACGGCTGGGGCAAGGACGGCGTCTT
CAACTTCGAAGGCGGCTGCTACGCCAAATGCATCAAGCTGTCGCCGGAAGCCGAGCCCGAGATCTTCGCGGCGTCGTCGC
GGTTCGGCGCGGTACTGGAAAACGTCGTGCTCGACGAGATCACCCGCAAGCCGGATTTCGACGACGGCTCCAAGACCGAG
AACACCCGGTCCGCCTATCCGCTGGAATCGATCCCGAACGCGTCGCTGACCGGCCGCGCCGGCCAGCCGAAGAACGTGGT
GATGCTCGCCGCCGACGCCTTCGGCGTGATGCCGCCGATCGCCAAGCTGACCCCGGCGCAGGCGATGTATCACTTCCTGT
CCGGCTACACCGCCAAGGTCGCCGGCACCGAGCGCGGCGTCACCGAGCCCGAGCCGGAATTCTCGACCTGCTTCGGCTCG
CCGTTCCTGCCGCGCGATCCGTCGGTCTACGGCAACATGCTGCGCGAACTGATCGCCAAGCACGATGTCGATTGCTGGCT
GGTCAACACCGGCTGGACCGGCGGCATCTACGGCACCGGCCACCGCATGCCGATCAAGGTCACCCGCGCGCTGCTGACCG
CGGCGCTCGACGGTAGCTTGCGCAACGTCGAATTCCGCACCGATCCGTATTTCGGCTTCGCGGTGCCGACGTCGCTGCAT
GGCGTGCCGAGCGACATCCTCGACCCGGTCAAGACCTGGGCCGACAAGGCCGCGTTCGATGCGACGGCGCGAAAACTGGT
CGGCATGTTCCAGAAGAACTTCGCCAAGTTTGAAGCCCAGGTCGACGCCGACGTCCGCGCCGCCGCGCCGGACGTCAAGA
TGGCGGCCGAGTAA

Upstream 100 bases:

>100_bases
CACTGGACCTGGGCGGTATATATAGAAGTTTACCGCGGGCTGAAGCGCATTGGCGGCAATCGCGCGATCGGGACAACGCG
GACTCGAGGAGGATTCATTC

Downstream 100 bases:

>100_bases
CTTCGAACGCCTCGGCTGGATCGAAGCGACATCGCGAATCAAAAAGGGCGGCCCATCATCGGGCCGCCCTTTTCCTTGGC
GGGCTGGCGCACCGAGCGCC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MQETGVHNGAHGADKFGLKNLKGVYWNFAAPQLYEHALKNGEAVLSADGALCADTGEFTGRSPKDKFTVRDATTETTMWW
GGNQSITAEQFETLYQDFLKHAEGMTLFAQDLYGGADPSFQIKTRVFTELAWHSLFIRTLLRRPDRAALESFVPELTLID
LPSFRADPKRHGCRSENVVAIDFARKIVLIGGTQYAGEMKKSVFTTLNYYLPEKGVMPMHCSANVGPQGDTAIFFGLSGT
GKTTLSADPNRTLIGDDEHGWGKDGVFNFEGGCYAKCIKLSPEAEPEIFAASSRFGAVLENVVLDEITRKPDFDDGSKTE
NTRSAYPLESIPNASLTGRAGQPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGVTEPEPEFSTCFGS
PFLPRDPSVYGNMLRELIAKHDVDCWLVNTGWTGGIYGTGHRMPIKVTRALLTAALDGSLRNVEFRTDPYFGFAVPTSLH
GVPSDILDPVKTWADKAAFDATARKLVGMFQKNFAKFEAQVDADVRAAAPDVKMAAE

Sequences:

>Translated_537_residues
MQETGVHNGAHGADKFGLKNLKGVYWNFAAPQLYEHALKNGEAVLSADGALCADTGEFTGRSPKDKFTVRDATTETTMWW
GGNQSITAEQFETLYQDFLKHAEGMTLFAQDLYGGADPSFQIKTRVFTELAWHSLFIRTLLRRPDRAALESFVPELTLID
LPSFRADPKRHGCRSENVVAIDFARKIVLIGGTQYAGEMKKSVFTTLNYYLPEKGVMPMHCSANVGPQGDTAIFFGLSGT
GKTTLSADPNRTLIGDDEHGWGKDGVFNFEGGCYAKCIKLSPEAEPEIFAASSRFGAVLENVVLDEITRKPDFDDGSKTE
NTRSAYPLESIPNASLTGRAGQPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGVTEPEPEFSTCFGS
PFLPRDPSVYGNMLRELIAKHDVDCWLVNTGWTGGIYGTGHRMPIKVTRALLTAALDGSLRNVEFRTDPYFGFAVPTSLH
GVPSDILDPVKTWADKAAFDATARKLVGMFQKNFAKFEAQVDADVRAAAPDVKMAAE
>Mature_537_residues
MQETGVHNGAHGADKFGLKNLKGVYWNFAAPQLYEHALKNGEAVLSADGALCADTGEFTGRSPKDKFTVRDATTETTMWW
GGNQSITAEQFETLYQDFLKHAEGMTLFAQDLYGGADPSFQIKTRVFTELAWHSLFIRTLLRRPDRAALESFVPELTLID
LPSFRADPKRHGCRSENVVAIDFARKIVLIGGTQYAGEMKKSVFTTLNYYLPEKGVMPMHCSANVGPQGDTAIFFGLSGT
GKTTLSADPNRTLIGDDEHGWGKDGVFNFEGGCYAKCIKLSPEAEPEIFAASSRFGAVLENVVLDEITRKPDFDDGSKTE
NTRSAYPLESIPNASLTGRAGQPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGVTEPEPEFSTCFGS
PFLPRDPSVYGNMLRELIAKHDVDCWLVNTGWTGGIYGTGHRMPIKVTRALLTAALDGSLRNVEFRTDPYFGFAVPTSLH
GVPSDILDPVKTWADKAAFDATARKLVGMFQKNFAKFEAQVDADVRAAAPDVKMAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=544, Percent_Identity=46.3235294117647, Blast_Score=461, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322950, Length=514, Percent_Identity=46.692607003891, Blast_Score=464, Evalue=1e-131,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_RHOP2 (Q2J2Y8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484083.1
- ProteinModelPortal:   Q2J2Y8
- SMR:   Q2J2Y8
- STRING:   Q2J2Y8
- GeneID:   3909806
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0461
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- ProtClustDB:   PRK09344
- BioCyc:   RPAL316058:RPB_0461-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58620; Mature: 58620

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETGVHNGAHGADKFGLKNLKGVYWNFAAPQLYEHALKNGEAVLSADGALCADTGEFTG
CCCCCCCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEECCCCEEECCCCCCC
RSPKDKFTVRDATTETTMWWGGNQSITAEQFETLYQDFLKHAEGMTLFAQDLYGGADPSF
CCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCE
QIKTRVFTELAWHSLFIRTLLRRPDRAALESFVPELTLIDLPSFRADPKRHGCRSENVVA
EEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCEEE
IDFARKIVLIGGTQYAGEMKKSVFTTLNYYLPEKGVMPMHCSANVGPQGDTAIFFGLSGT
EEECCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEECCC
GKTTLSADPNRTLIGDDEHGWGKDGVFNFEGGCYAKCIKLSPEAEPEIFAASSRFGAVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCEEECCHHHHHHHH
NVVLDEITRKPDFDDGSKTENTRSAYPLESIPNASLTGRAGQPKNVVMLAADAFGVMPPI
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECHHCCCCCH
AKLTPAQAMYHFLSGYTAKVAGTERGVTEPEPEFSTCFGSPFLPRDPSVYGNMLRELIAK
HHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHH
HDVDCWLVNTGWTGGIYGTGHRMPIKVTRALLTAALDGSLRNVEFRTDPYFGFAVPTSLH
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCC
GVPSDILDPVKTWADKAAFDATARKLVGMFQKNFAKFEAQVDADVRAAAPDVKMAAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MQETGVHNGAHGADKFGLKNLKGVYWNFAAPQLYEHALKNGEAVLSADGALCADTGEFTG
CCCCCCCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEECCCCEEECCCCCCC
RSPKDKFTVRDATTETTMWWGGNQSITAEQFETLYQDFLKHAEGMTLFAQDLYGGADPSF
CCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCE
QIKTRVFTELAWHSLFIRTLLRRPDRAALESFVPELTLIDLPSFRADPKRHGCRSENVVA
EEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCEEE
IDFARKIVLIGGTQYAGEMKKSVFTTLNYYLPEKGVMPMHCSANVGPQGDTAIFFGLSGT
EEECCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEECCC
GKTTLSADPNRTLIGDDEHGWGKDGVFNFEGGCYAKCIKLSPEAEPEIFAASSRFGAVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCEEECCHHHHHHHH
NVVLDEITRKPDFDDGSKTENTRSAYPLESIPNASLTGRAGQPKNVVMLAADAFGVMPPI
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECHHCCCCCH
AKLTPAQAMYHFLSGYTAKVAGTERGVTEPEPEFSTCFGSPFLPRDPSVYGNMLRELIAK
HHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHH
HDVDCWLVNTGWTGGIYGTGHRMPIKVTRALLTAALDGSLRNVEFRTDPYFGFAVPTSLH
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCC
GVPSDILDPVKTWADKAAFDATARKLVGMFQKNFAKFEAQVDADVRAAAPDVKMAAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA