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The map label for this gene is pfkB [H]

Identifier: 86747570

GI number: 86747570

Start: 490104

End: 491042

Strand: Reverse

Name: pfkB [H]

Synonym: RPB_0444

Alternate gene names: 86747570

Gene position: 491042-490104 (Counterclockwise)

Preceding gene: 86747572

Following gene: 86747569

Centisome position: 9.21

GC content: 70.39

Gene sequence:

>939_bases
ATGGTCGAGATCGTGACCCTCACGCCCAATCCCGCCATCGATCTGTCGACCTCGGTCGCCCGCATGGTGCCGAGCCGCAA
GCTGCGCTGCGCGGCGCAGCAGCGTGATCCCGGCGGCGGCGGCATCAATGTGGCGCGGGTGGTGAAGCGGTTCGGCGGCG
ACGTCGAGGCGATCTATCCGGCCGGCGGCTTCACCGGCCGGCTGCTGCGCGCGCTGCTCGACCGCGAGCAGATCCCGAGC
CGCGTCGTCGAGGCGGTGGCCGAGACCCGCGAGGATTTCTCCGTCACGGAATCAGAGACCGGCCAGCAGTTCCGCTTCGT
GCTGCCGGGCGAGGCCTTGGCCGAGGGCGAGTGGCGCGGGCTGCTGGAGATTCTCGGCGGGACCGATCCGGCACCGACCA
TCGTGGTCGGCTCGGGCAGCCTGCCGCCCGGCGTGCCGCATGATTTCTACGCCCAGGCCGCCGCGGTGGCGAAGCGGATC
GGCGCGCAGTTCTTTCTCGACACCTCCGGCGACCAGCTGATCGCCGCGCTCGCTCATGGCGTCACGCTGATCAAACCGAA
TCTGCGCGAGATGCAGGAATTGGCCGGCCGGCCGCTCGCCAATGATCAGGACTACGTCGCCGCCGCGCGGCAGCAGATCG
ACGCCGGCAAGGTCGAGATCGTCGCGCTGTCGCTCGGCCATCTCGGCGCGCTGCTGGTCACCAAGGACAAGGCGCTGCGT
TCGCCGGCGCTGCCGGTCGCGCAGGTCTCCACCGTCGGCGCCGGCGACAGTTTCCTCGGCGCGATCGCCTTCAGCCTGGC
CAAGGGGCGGCCGATCGAGGACGCCTTCCGGCTGGCGCTGGCTTCGGGCTCGGCGTCGCTCACCATGCACGGCACCGAAT
TGTGCCGGCCGGCCGATGTCGAACGATTGAGCGCCGAGGTGGTGATCCAGCCGCTGTAG

Upstream 100 bases:

>100_bases
GCGTGAGACTGCGGCGTCCGCCCGACGTCCCGTCGCGGCAGCCGGCGGTGCCGTCCGCCGGTCCTCATTCCCCGCAAGCC
AGAACTGGAGATCGTCGCCG

Downstream 100 bases:

>100_bases
CGCGCGCCCGGCGCTCACATTGCGCGGAGCGGCCAAGGCCGCTCCGGAACCGTTGTGGGGACGGCCGGTTGTGTTCCGCG
ACGCCGCATTGCGCGTCGCG

Product: PfkB

Products: ADP; D-fructose 1,6-bisphosphate

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MVEIVTLTPNPAIDLSTSVARMVPSRKLRCAAQQRDPGGGGINVARVVKRFGGDVEAIYPAGGFTGRLLRALLDREQIPS
RVVEAVAETREDFSVTESETGQQFRFVLPGEALAEGEWRGLLEILGGTDPAPTIVVGSGSLPPGVPHDFYAQAAAVAKRI
GAQFFLDTSGDQLIAALAHGVTLIKPNLREMQELAGRPLANDQDYVAAARQQIDAGKVEIVALSLGHLGALLVTKDKALR
SPALPVAQVSTVGAGDSFLGAIAFSLAKGRPIEDAFRLALASGSASLTMHGTELCRPADVERLSAEVVIQPL

Sequences:

>Translated_312_residues
MVEIVTLTPNPAIDLSTSVARMVPSRKLRCAAQQRDPGGGGINVARVVKRFGGDVEAIYPAGGFTGRLLRALLDREQIPS
RVVEAVAETREDFSVTESETGQQFRFVLPGEALAEGEWRGLLEILGGTDPAPTIVVGSGSLPPGVPHDFYAQAAAVAKRI
GAQFFLDTSGDQLIAALAHGVTLIKPNLREMQELAGRPLANDQDYVAAARQQIDAGKVEIVALSLGHLGALLVTKDKALR
SPALPVAQVSTVGAGDSFLGAIAFSLAKGRPIEDAFRLALASGSASLTMHGTELCRPADVERLSAEVVIQPL
>Mature_312_residues
MVEIVTLTPNPAIDLSTSVARMVPSRKLRCAAQQRDPGGGGINVARVVKRFGGDVEAIYPAGGFTGRLLRALLDREQIPS
RVVEAVAETREDFSVTESETGQQFRFVLPGEALAEGEWRGLLEILGGTDPAPTIVVGSGSLPPGVPHDFYAQAAAVAKRI
GAQFFLDTSGDQLIAALAHGVTLIKPNLREMQELAGRPLANDQDYVAAARQQIDAGKVEIVALSLGHLGALLVTKDKALR
SPALPVAQVSTVGAGDSFLGAIAFSLAKGRPIEDAFRLALASGSASLTMHGTELCRPADVERLSAEVVIQPL

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=310, Percent_Identity=42.5806451612903, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1788493, Length=317, Percent_Identity=29.3375394321767, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11

Molecular weight: Translated: 32787; Mature: 32787

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEIVTLTPNPAIDLSTSVARMVPSRKLRCAAQQRDPGGGGINVARVVKRFGGDVEAIYP
CEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEC
AGGFTGRLLRALLDREQIPSRVVEAVAETREDFSVTESETGQQFRFVLPGEALAEGEWRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHCCCHHHH
LLEILGGTDPAPTIVVGSGSLPPGVPHDFYAQAAAVAKRIGAQFFLDTSGDQLIAALAHG
HHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCC
VTLIKPNLREMQELAGRPLANDQDYVAAARQQIDAGKVEIVALSLGHLGALLVTKDKALR
EEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCHHHC
SPALPVAQVSTVGAGDSFLGAIAFSLAKGRPIEDAFRLALASGSASLTMHGTELCRPADV
CCCCCHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCHHHCCCCCH
ERLSAEVVIQPL
HHHCEEEEEECC
>Mature Secondary Structure
MVEIVTLTPNPAIDLSTSVARMVPSRKLRCAAQQRDPGGGGINVARVVKRFGGDVEAIYP
CEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEC
AGGFTGRLLRALLDREQIPSRVVEAVAETREDFSVTESETGQQFRFVLPGEALAEGEWRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHCCCHHHH
LLEILGGTDPAPTIVVGSGSLPPGVPHDFYAQAAAVAKRIGAQFFLDTSGDQLIAALAHG
HHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCC
VTLIKPNLREMQELAGRPLANDQDYVAAARQQIDAGKVEIVALSLGHLGALLVTKDKALR
EEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCHHHC
SPALPVAQVSTVGAGDSFLGAIAFSLAKGRPIEDAFRLALASGSASLTMHGTELCRPADV
CCCCCHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCHHHCCCCCH
ERLSAEVVIQPL
HHHCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose 6-phosphate

Specific reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]