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The map label for this gene is rph [H]

Identifier: 86747551

GI number: 86747551

Start: 467984

End: 468697

Strand: Reverse

Name: rph [H]

Synonym: RPB_0425

Alternate gene names: 86747551

Gene position: 468697-467984 (Counterclockwise)

Preceding gene: 86747554

Following gene: 86747550

Centisome position: 8.79

GC content: 68.49

Gene sequence:

>714_bases
ATGCGGCCAAGCCGCCGTGCGCCCGACGAACTGCGCGCCGTCTCGCTGGAGCGGGGCGTCGTGAAATACGCCGAGGGCTC
CTGCCTGGTTAAATTCGGCGACACCCATGTGCTGGTGACGGCGACCCTGGAGGAGCGGCTGCCGCCGTGGCTGAAGGGCC
AGGGCCGTGGCTGGGTGACGGCCGAATACGGCATGCTGCCCCGCGCCACGCTGGAACGCACCCGGCGCGAGGCCGCAGCC
GGCAAGCAGAATGGCCGCACCGTCGAGATCCAGCGCCTGATCGGCCGCTCGCTGCGCACCATCGTCGACCTGCAGGCGCT
CGGCGAGCGCCAGATCACGGTCGATTGCGACGTGCTGCAGGCCGATGGCGGCACCCGGACTGCCTCGATCACCGGCGCCT
GGGTGGCGCTGGCCGACTGTCTCGCCTGGATGAAGACCCGCAACATGCTCAAAGGCCAGGTGATGCGCGACAACGTCGCA
GCGATCTCCTGCGGAATCTACAACGGCACCCCGGTGCTCGACCTCGACTACGCCGAGGACTCCGAAGCCGAAACCGACGC
CAATTTCGTCATGACCGGCGACGGCCGGATCGTCGAGGTGCAGGGCACTGCCGAGAAGACGCCGTTCTCGCAGGACGAGT
TCCTGGCGCTGATGGCGCTGGCGCAGAAGGGCGTGGCGCGGCTGGTCGATCTGCAGAAAATGGCGGTGGCGTGA

Upstream 100 bases:

>100_bases
GCGCAAGGACGGCTGTTCTGCCAGCGCCCTTGCCGCTGCGCCGCTCCGCCCCTAAAAGGCAGCCAGATCGCCATTTCCCG
AAATTGACGGAGGATTTCCC

Downstream 100 bases:

>100_bases
GTCATTCTTGCCACCCATTCGCGGCCGCTCCTGAGCTACGCTAGCGCCGCCATGCATCGACGAATCAGCGGCCAGCTCGT
GATCGCGACCCACAATCCCG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVTAEYGMLPRATLERTRREAAA
GKQNGRTVEIQRLIGRSLRTIVDLQALGERQITVDCDVLQADGGTRTASITGAWVALADCLAWMKTRNMLKGQVMRDNVA
AISCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRIVEVQGTAEKTPFSQDEFLALMALAQKGVARLVDLQKMAVA

Sequences:

>Translated_237_residues
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVTAEYGMLPRATLERTRREAAA
GKQNGRTVEIQRLIGRSLRTIVDLQALGERQITVDCDVLQADGGTRTASITGAWVALADCLAWMKTRNMLKGQVMRDNVA
AISCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRIVEVQGTAEKTPFSQDEFLALMALAQKGVARLVDLQKMAVA
>Mature_237_residues
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVTAEYGMLPRATLERTRREAAA
GKQNGRTVEIQRLIGRSLRTIVDLQALGERQITVDCDVLQADGGTRTASITGAWVALADCLAWMKTRNMLKGQVMRDNVA
AISCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRIVEVQGTAEKTPFSQDEFLALMALAQKGVARLVDLQKMAVA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=63.9810426540284, Blast_Score=267, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71981632, Length=182, Percent_Identity=24.7252747252747, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25897; Mature: 25897

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVT
CCCCCCCCHHHHHHHHHCCEEEECCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCEEEE
AEYGMLPRATLERTRREAAAGKQNGRTVEIQRLIGRSLRTIVDLQALGERQITVDCDVLQ
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAWVALADCLAWMKTRNMLKGQVMRDNVAAISCGIYNGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEECCCCCCEEEEECCCC
SEAETDANFVMTGDGRIVEVQGTAEKTPFSQDEFLALMALAQKGVARLVDLQKMAVA
CCCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEERLPPWLKGQGRGWVT
CCCCCCCCHHHHHHHHHCCEEEECCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCEEEE
AEYGMLPRATLERTRREAAAGKQNGRTVEIQRLIGRSLRTIVDLQALGERQITVDCDVLQ
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAWVALADCLAWMKTRNMLKGQVMRDNVAAISCGIYNGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEECCCCCCEEEEECCCC
SEAETDANFVMTGDGRIVEVQGTAEKTPFSQDEFLALMALAQKGVARLVDLQKMAVA
CCCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA