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The map label for this gene is hslU

Identifier: 86747529

GI number: 86747529

Start: 447864

End: 449165

Strand: Reverse

Name: hslU

Synonym: RPB_0403

Alternate gene names: 86747529

Gene position: 449165-447864 (Counterclockwise)

Preceding gene: 86747530

Following gene: 86747525

Centisome position: 8.42

GC content: 66.13

Gene sequence:

>1302_bases
ATGACCGACTTCTCACCCCGCGAAATCGTTTCCGAACTCGACCGCTTCATCGTCGGCCAGGCCGACGCCAAGCGCGCGGT
CGCCATCGCGCTGCGCAACCGCTGGCGGCGGCTGCAGCTCGAAGGCGCGCTGCGCGAAGAAGTGCTGCCGAAGAACATCC
TGATGATCGGGCCGACCGGCGTCGGCAAGACCGAGATCGCGCGCCGGCTGGCCAAGCTCGCCAACGCACCGTTCCTCAAA
GTCGAGGCCACCAAATTCACCGAGGTCGGCTATGTCGGCCGCGACGTCGAGCAGATCATCCGCGATCTGGTCGAAGTGGC
GATCGCGCAGGTGCGCGAGAAGAAGCGCAAGGACGTCCAGGCCCGCGCCCAGATCGCCGCCGAGGAACGCGTGCTCGATG
CGCTGGTCGGCCCGGGATCGAGCCCGGCGACGCGGGACTCGTTTCGCCGCAAGCTGCGGACCGGCGAGCTCAACGACAAG
GAAATCGAAATCGAGACCCAGGCCGGCGGCGGCTCGCCGATGTTCGAAATTCCGGGCATGCCGGGCGGCCAGATCGGCGC
GATTTCGATCGGCGACATCTTCGGCAAGATGGGTGGCCGCACCAAGACGCGCAGGCTCACCGTCGTCGATTCGCACGACA
TCCTCGTCAACGAGGAAGCCGACAAGCTGCTCGACAATGACCAGCTGGTGCAGGAAGCCATCAACGCCGTCGAGAACAAC
GGCATCGTGTTTCTCGACGAGATCGACAAGATCTGCGTGCGCGACGGCCGCAGCGGCGGCGAGGTCTCGCGCGAGGGCGT
GCAGCGCGATCTGCTGCCGCTGATCGAAGGCACCACGGTCGCCACCAAGCACGGCGCGGTGAAGACCGATCACATCCTGT
TCATCGCCTCCGGCGCGTTCCACATCGCCAAGCCGTCCGACCTGCTGCCGGAGCTGCAGGGCCGGCTGCCGATCCGGGTC
GAGCTCAACGCACTCTCCCGCGACGACATGCGCCGGATTCTGACCGAGCCGGAAGCCTCGCTGATCAAGCAATATGTGGC
GCTGCTGCAGACCGAAGGCGTGACGCTGGAATTCGGCGACGACGCCATCGACGCGCTCGCCGACGTCGCGGTCGCGGTCA
ACTCCACCGTCGAGAACATCGGCGCGCGGCGGCTGCAGACGGTGATGGAGCGCGTGCTCGACGACATCTCCTTCGGCGCG
CCGGACCGAGGCGGCGAGACCATCCGGATCGACGCCGACTACGTCCAGAAGAACGTCGGCGATCTGGCGAAGAACACGGA
TTTGAGCCGGTTCATCTTGTAG

Upstream 100 bases:

>100_bases
GGGTGAGGGGCAGCCTCTCCGTCTCGCGACACCCCTCACCCGGCACGGACTTCGTCCGCGCCACCCTCTCCCACAAGGGG
AGAGGGAAAGAAGTAGAATC

Downstream 100 bases:

>100_bases
TTGAGCGTCGGGCGTTCCTCTGCTCCCACGAACGCCACCACAAACACACACGTCATGCGCGGGCTTGACCCGCGCATCCA
TCGCTTTTCGCAAGACGATG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGALREEVLPKNILMIGPTGVGKTEIARRLAKLANAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQARAQIAAEERVLDALVGPGSSPATRDSFRRKLRTGELNDK
EIEIETQAGGGSPMFEIPGMPGGQIGAISIGDIFGKMGGRTKTRRLTVVDSHDILVNEEADKLLDNDQLVQEAINAVENN
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVATKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRV
ELNALSRDDMRRILTEPEASLIKQYVALLQTEGVTLEFGDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFGA
PDRGGETIRIDADYVQKNVGDLAKNTDLSRFIL

Sequences:

>Translated_433_residues
MTDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGALREEVLPKNILMIGPTGVGKTEIARRLAKLANAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQARAQIAAEERVLDALVGPGSSPATRDSFRRKLRTGELNDK
EIEIETQAGGGSPMFEIPGMPGGQIGAISIGDIFGKMGGRTKTRRLTVVDSHDILVNEEADKLLDNDQLVQEAINAVENN
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVATKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRV
ELNALSRDDMRRILTEPEASLIKQYVALLQTEGVTLEFGDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFGA
PDRGGETIRIDADYVQKNVGDLAKNTDLSRFIL
>Mature_432_residues
TDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGALREEVLPKNILMIGPTGVGKTEIARRLAKLANAPFLKV
EATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQARAQIAAEERVLDALVGPGSSPATRDSFRRKLRTGELNDKE
IEIETQAGGGSPMFEIPGMPGGQIGAISIGDIFGKMGGRTKTRRLTVVDSHDILVNEEADKLLDNDQLVQEAINAVENNG
IVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVATKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVE
LNALSRDDMRRILTEPEASLIKQYVALLQTEGVTLEFGDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFGAP
DRGGETIRIDADYVQKNVGDLAKNTDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=59.6412556053812, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1786642, Length=215, Percent_Identity=36.2790697674419, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=498, Percent_Identity=25.9036144578313, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648291, Length=257, Percent_Identity=30.7392996108949, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648289, Length=257, Percent_Identity=30.7392996108949, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_RHOP2 (Q2J346)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484025.1
- ProteinModelPortal:   Q2J346
- SMR:   Q2J346
- STRING:   Q2J346
- GeneID:   3908841
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0403
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   RPAL316058:RPB_0403-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 47452; Mature: 47321

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 246-246 BINDING 311-311 BINDING 383-383

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGALREEVLPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
VGKTEIARRLAKLANAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQIAAEERVLDALVGPGSSPATRDSFRRKLRTGELNDKEIEIETQAGGGSPMFEIPGM
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCC
PGGQIGAISIGDIFGKMGGRTKTRRLTVVDSHDILVNEEADKLLDNDQLVQEAINAVENN
CCCCEEEEEHHHHHHHCCCCCCCEEEEEEECCCEEEECCHHHHHCCHHHHHHHHHHHHCC
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVATKHGAVKTDHILFIASGAF
CEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEECEEEEEECCCE
HIAKPSDLLPELQGRLPIRVELNALSRDDMRRILTEPEASLIKQYVALLQTEGVTLEFGD
EECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECC
DAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFGAPDRGGETIRIDADYVQKNVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHH
DLAKNTDLSRFIL
HHHHCCCHHHHCC
>Mature Secondary Structure 
TDFSPREIVSELDRFIVGQADAKRAVAIALRNRWRRLQLEGALREEVLPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
VGKTEIARRLAKLANAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVAIAQVREKKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQIAAEERVLDALVGPGSSPATRDSFRRKLRTGELNDKEIEIETQAGGGSPMFEIPGM
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCC
PGGQIGAISIGDIFGKMGGRTKTRRLTVVDSHDILVNEEADKLLDNDQLVQEAINAVENN
CCCCEEEEEHHHHHHHCCCCCCCEEEEEEECCCEEEECCHHHHHCCHHHHHHHHHHHHCC
GIVFLDEIDKICVRDGRSGGEVSREGVQRDLLPLIEGTTVATKHGAVKTDHILFIASGAF
CEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEECEEEEEECCCE
HIAKPSDLLPELQGRLPIRVELNALSRDDMRRILTEPEASLIKQYVALLQTEGVTLEFGD
EECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECC
DAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFGAPDRGGETIRIDADYVQKNVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHH
DLAKNTDLSRFIL
HHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA