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The map label for this gene is gsiB [H]

Identifier: 86747358

GI number: 86747358

Start: 264849

End: 266402

Strand: Reverse

Name: gsiB [H]

Synonym: RPB_0232

Alternate gene names: 86747358

Gene position: 266402-264849 (Counterclockwise)

Preceding gene: 86747359

Following gene: 86747357

Centisome position: 5.0

GC content: 63.77

Gene sequence:

>1554_bases
ATGCGCATACTCGATCTGGGATCACGAACCCTGCGACGCCGCGATATTCTGGCGCTGATCGGCGGCGGCGCCGCAGCCGC
TGCGGTCGGCCTGCCGGCGCTGGCGCAGGAGCCCAAGAAGGGCGGCGTGCTGAAGGTCGCGGCCCCAGCGAATCCGTCGT
CGCTCGATCCGGCCACCGGCGGCGCCGGTTCCGACCACAGCATTCTCTGGACGATCTACGATACGCTGGTCGAGTGGGAC
TACGACACGCTGAAGCCGAGGCCCGGCATGGCGAAATGGTCGTATCCGAATCCGACCACGATGGTGATCGACATCACCCC
CGGCATCCAGTTCCACGACGGCACCGCGATGGACGCCGAGGCGGTGAAGTTCAACCTCGATCGCAACCGCTCCGATCAGC
GGTCCAATATCAAGTCGGATCTCGCCAGCATCGAGTCGATCGAGGTGACCAGCCCGCTGCAGGTGACGCTGAAGCTGAAG
AGCCCGGATACATCCCTGCCGGCGATCCTGTCCGACCGCGCCGGCATGATGGTGTCGCCGACCAACATCAAGGCGCTTGG
CAACGAGACAGACCGCAAGCCGGTCGGCGCCGGGCCGTGGAAGTTCGTGCGCTGGAACGACAACGAAATCATCGTCGTGG
CTCGCCACGAGAACTACTGGCGCAAGGGCCGGCCGTATCTCGACGGCATCGAGTTCAACATCATCACCGAAAACGCCACG
GCGCTGCGGTCGGTGGTCGCCGGCCAGAACGACATGGCATTTCAGTTGCCGGCACGGCTGAAGCCGGTGATTGAGCGCGC
CAAGGACCTGACCATGGTCAGCTCGCCGACGCTGTATTGCATTCAGGTGTATTTCAACTACGCCCGCGCGCCGCTCGACA
ATCTCAAGGTTCGTCAGGCGATCAATTTCGCGTTCGACCGCGACACCTTCGTCAAGGCGGCGCTGAGCGGGCTCGGCGAA
TCGGCCCGGATGACGCTGCCGAGCTCGCACTGGGCGTTCAACAAGGATGTGGCCGGCACCTATCCGCACGATCCGGAGAA
GGCGAAGAAGTTGCTGGCAGAGGCCGGCTACAAGGACGGCCTCGAGCTGACGATCGGCGGCTATACCGATCAGGATTCGG
TGCGCCGCGGCGAGGTGATCCAGGATCAGCTCGGCAAGGTCGGCATCCGGCTCAAATTCACCCGCGGCACCATCGCGGAA
ATCAGCGCGCAGTTCTTCGCGCAGGAGAAGAAGTTCGACCTGTTGGTGTCGGCCTGGACCGGGCGTCCCGATCCGAGCAT
GACCTATGGGCTCGGCTTCGACAAAGGCGCGTACTACAACGCCGGCCGCACCGCCGATCCTGAGCTGTCCAAGCTGATCC
TCGAAAGCCGCGTCAGCGAGGATTTGGCCAAGCGCGCCGAAGTGTTCGCCAGGATCCAGCGCATCACGGTCGAACAGGCA
CTGTCGGCGCCGCTGGCGTTCCAGTTCGAGCTCGACGCGCTGTCGTCCAAGGTGAAGGGCTTCAAGCCCAATCTGCTCGG
CAAGCCGAAGTTCGAATACATCTCCCTCGCGTGA

Upstream 100 bases:

>100_bases
CTTCGCGACGGCCGAAGGGCCGCCGGCCGACAATCAACAGGCGCGATCCGCGTCGTAATCAACATCAAGATCCAAAAATC
AGGATCATCGGGAGGACGAC

Downstream 100 bases:

>100_bases
GTGAACGGCGCCGCATGGTTTGCGGCGCCCGTTCCGGATCGGGTCCTCATGTTGAACGCTGCACGTCTTCACATCGTCGG
ACGCCGGGTGCTGCAGGCGA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MRILDLGSRTLRRRDILALIGGGAAAAAVGLPALAQEPKKGGVLKVAAPANPSSLDPATGGAGSDHSILWTIYDTLVEWD
YDTLKPRPGMAKWSYPNPTTMVIDITPGIQFHDGTAMDAEAVKFNLDRNRSDQRSNIKSDLASIESIEVTSPLQVTLKLK
SPDTSLPAILSDRAGMMVSPTNIKALGNETDRKPVGAGPWKFVRWNDNEIIVVARHENYWRKGRPYLDGIEFNIITENAT
ALRSVVAGQNDMAFQLPARLKPVIERAKDLTMVSSPTLYCIQVYFNYARAPLDNLKVRQAINFAFDRDTFVKAALSGLGE
SARMTLPSSHWAFNKDVAGTYPHDPEKAKKLLAEAGYKDGLELTIGGYTDQDSVRRGEVIQDQLGKVGIRLKFTRGTIAE
ISAQFFAQEKKFDLLVSAWTGRPDPSMTYGLGFDKGAYYNAGRTADPELSKLILESRVSEDLAKRAEVFARIQRITVEQA
LSAPLAFQFELDALSSKVKGFKPNLLGKPKFEYISLA

Sequences:

>Translated_517_residues
MRILDLGSRTLRRRDILALIGGGAAAAAVGLPALAQEPKKGGVLKVAAPANPSSLDPATGGAGSDHSILWTIYDTLVEWD
YDTLKPRPGMAKWSYPNPTTMVIDITPGIQFHDGTAMDAEAVKFNLDRNRSDQRSNIKSDLASIESIEVTSPLQVTLKLK
SPDTSLPAILSDRAGMMVSPTNIKALGNETDRKPVGAGPWKFVRWNDNEIIVVARHENYWRKGRPYLDGIEFNIITENAT
ALRSVVAGQNDMAFQLPARLKPVIERAKDLTMVSSPTLYCIQVYFNYARAPLDNLKVRQAINFAFDRDTFVKAALSGLGE
SARMTLPSSHWAFNKDVAGTYPHDPEKAKKLLAEAGYKDGLELTIGGYTDQDSVRRGEVIQDQLGKVGIRLKFTRGTIAE
ISAQFFAQEKKFDLLVSAWTGRPDPSMTYGLGFDKGAYYNAGRTADPELSKLILESRVSEDLAKRAEVFARIQRITVEQA
LSAPLAFQFELDALSSKVKGFKPNLLGKPKFEYISLA
>Mature_517_residues
MRILDLGSRTLRRRDILALIGGGAAAAAVGLPALAQEPKKGGVLKVAAPANPSSLDPATGGAGSDHSILWTIYDTLVEWD
YDTLKPRPGMAKWSYPNPTTMVIDITPGIQFHDGTAMDAEAVKFNLDRNRSDQRSNIKSDLASIESIEVTSPLQVTLKLK
SPDTSLPAILSDRAGMMVSPTNIKALGNETDRKPVGAGPWKFVRWNDNEIIVVARHENYWRKGRPYLDGIEFNIITENAT
ALRSVVAGQNDMAFQLPARLKPVIERAKDLTMVSSPTLYCIQVYFNYARAPLDNLKVRQAINFAFDRDTFVKAALSGLGE
SARMTLPSSHWAFNKDVAGTYPHDPEKAKKLLAEAGYKDGLELTIGGYTDQDSVRRGEVIQDQLGKVGIRLKFTRGTIAE
ISAQFFAQEKKFDLLVSAWTGRPDPSMTYGLGFDKGAYYNAGRTADPELSKLILESRVSEDLAKRAEVFARIQRITVEQA
LSAPLAFQFELDALSSKVKGFKPNLLGKPKFEYISLA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=511, Percent_Identity=27.0058708414873, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789966, Length=546, Percent_Identity=25.6410256410256, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787762, Length=511, Percent_Identity=24.4618395303327, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787551, Length=536, Percent_Identity=22.5746268656716, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789887, Length=515, Percent_Identity=25.631067961165, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081878, Length=496, Percent_Identity=21.1693548387097, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56826; Mature: 56826

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILDLGSRTLRRRDILALIGGGAAAAAVGLPALAQEPKKGGVLKVAAPANPSSLDPATG
CEEEECCCHHHHHCCEEEEECCCHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCCCCC
GAGSDHSILWTIYDTLVEWDYDTLKPRPGMAKWSYPNPTTMVIDITPGIQFHDGTAMDAE
CCCCCCCEEEEEEHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCE
AVKFNLDRNRSDQRSNIKSDLASIESIEVTSPLQVTLKLKSPDTSLPAILSDRAGMMVSP
EEEEECCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEEECCCCCCCCHHHHCCCCCEECC
TNIKALGNETDRKPVGAGPWKFVRWNDNEIIVVARHENYWRKGRPYLDGIEFNIITENAT
CCEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCHHHCCCCCCCCEEEEEEECCHH
ALRSVVAGQNDMAFQLPARLKPVIERAKDLTMVSSPTLYCIQVYFNYARAPLDNLKVRQA
HHHHHHCCCCCCEEECCHHHHHHHHHHHHCEEECCCCEEEEEEHHHHHHCCHHHHHHHHH
INFAFDRDTFVKAALSGLGESARMTLPSSHWAFNKDVAGTYPHDPEKAKKLLAEAGYKDG
HHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
LELTIGGYTDQDSVRRGEVIQDQLGKVGIRLKFTRGTIAEISAQFFAQEKKFDLLVSAWT
CEEEECCCCCCCHHHCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEEC
GRPDPSMTYGLGFDKGAYYNAGRTADPELSKLILESRVSEDLAKRAEVFARIQRITVEQA
CCCCCCEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSAPLAFQFELDALSSKVKGFKPNLLGKPKFEYISLA
HCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MRILDLGSRTLRRRDILALIGGGAAAAAVGLPALAQEPKKGGVLKVAAPANPSSLDPATG
CEEEECCCHHHHHCCEEEEECCCHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCCCCC
GAGSDHSILWTIYDTLVEWDYDTLKPRPGMAKWSYPNPTTMVIDITPGIQFHDGTAMDAE
CCCCCCCEEEEEEHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCE
AVKFNLDRNRSDQRSNIKSDLASIESIEVTSPLQVTLKLKSPDTSLPAILSDRAGMMVSP
EEEEECCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEEECCCCCCCCHHHHCCCCCEECC
TNIKALGNETDRKPVGAGPWKFVRWNDNEIIVVARHENYWRKGRPYLDGIEFNIITENAT
CCEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCHHHCCCCCCCCEEEEEEECCHH
ALRSVVAGQNDMAFQLPARLKPVIERAKDLTMVSSPTLYCIQVYFNYARAPLDNLKVRQA
HHHHHHCCCCCCEEECCHHHHHHHHHHHHCEEECCCCEEEEEEHHHHHHCCHHHHHHHHH
INFAFDRDTFVKAALSGLGESARMTLPSSHWAFNKDVAGTYPHDPEKAKKLLAEAGYKDG
HHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
LELTIGGYTDQDSVRRGEVIQDQLGKVGIRLKFTRGTIAEISAQFFAQEKKFDLLVSAWT
CEEEECCCCCCCHHHCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEEC
GRPDPSMTYGLGFDKGAYYNAGRTADPELSKLILESRVSEDLAKRAEVFARIQRITVEQA
CCCCCCEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSAPLAFQFELDALSSKVKGFKPNLLGKPKFEYISLA
HCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA