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The map label for this gene is hbpA [H]

Identifier: 86747351

GI number: 86747351

Start: 253123

End: 254784

Strand: Reverse

Name: hbpA [H]

Synonym: RPB_0225

Alternate gene names: 86747351

Gene position: 254784-253123 (Counterclockwise)

Preceding gene: 86747353

Following gene: 86747350

Centisome position: 4.78

GC content: 65.46

Gene sequence:

>1662_bases
ATGCGCCATCTTCATTCCTTCAGCCGCACTGCGGTGCTGCTCGCTCTGACGGCCGTCGGCTCTGTCGCCGTGCCGCAGAT
CGCTTCATCCGAAACCGTGCTGCGGATCGGCATGACGGCCGCGGACATTCCGCGCACGCTCGGCCAGCCGGATCAGGGTT
TCGAAGGCAACCGCTTCACCGGCCTGACGATGTATGACGGGCTGACGATGTGGGACCTGTCGTCCGCCACCAAGGCGAGC
GTGGTGATCCCGGGGCTCGCGACCGAATGGAAGGTCGAGGACGCCGACAAGACCAAATGGATCTTCAAGCTGCGCCCCGG
CGTCACCTTCCACGACGGCACGCCTTTCAATGCCGACGCCGTGGTCTGGAATGTCGACAAGGTGCTGAACAAGGAGGCTG
TTCAGTTCGACGCCAGCCAGGTCGGCGTCACCGCGTCGCGGATGCCGACGCTGGTCTCGGCCAAGAAGATCGACGACATG
ACGGTGGAGCTCACCGCCAAGGAGCCCGACAGCTTCCTGCCGATCAACCTCACCAATCTGTTCATCGTCAGCCCGTCGAA
ATGGCAGGCGCTGTACGAGAAGGCCGAGGGCGCCGACGCCAAGGCGCGGTCGCAGGCCGCCTGGGCCGCGTTCGCCAAGG
ACGCCGCCGGCACCGGGCCGTGGAAGATGGCGAAGTTCACGCCGCGCGAGCGGCTCGAACTGGTGAAGAACGACAATTAC
TGGGACAAGGCGCGGGTGCCGAAGACCGACCGCATGGTGCTGCTGCCGATGCCCGAAGCCAACGCGCGCACCGCGGCGCT
GCTGTCCGGCCAGGTCGACTGGATCGAGGCGCCTGCGCCCGACGCGGTGAAGGAGATCACCGCGCGCGGCTTCAAGATCG
AGAAGAACGAACAGCCGCATGTCTGGCCGTGGCAGTTCTCGCGGATCGAGGGCTCGCCCTGGAACGACATCCGCGTCCGC
CGCGCCGCCAATCTGTGCATCGACCGCGAAGGCCTGCGTGACGGCCTGCTCGCCGGCCTGATGGTGCCGGCGACCGGCAC
CTTCGAGCCCGGCCATCCGTGGCGCGGCAATCCGTCGTTCCAGATCAAGTACGATCTGCCGGCGGCGCAGAAGCTGATGA
AGGAGGCCGGCTTCGGCCCCGACAAGAAGCTCAGCGTCAAGGTGCAGACCTCGGCGTCCGGCTCCGGCCAGATGCTGCCG
CTGCCGATGAACGAATATCTGCAGCAGGCTCTCGCCGAGTGTTACTTCGACGTCAAGCTCGATGTCATCGAATGGAACAC
GCTGTTCACCAATTGGCGGCGTGGCGTCAAGGATCCCTCGGCCAACGGCAGCAACGCCACCAATGTCACCTACGCGGCGA
TGGATCCGTTCTTCGCGCTGGTCCGCTTCCTGCAATCGTCGATGGCGCCGCCGGTGTCGAACAATTGGGGCTACATCAAC
AACCCCAAGTTCGACGAGTTGGTGAAGAAGGCACGGACCAGCTTCGACGCCGCCGAGCGCGACAAGGCGCTGGCCGAGCT
GAACGCCGCCTCGATCGACGACGCGGCCTTCCTCTACGTCGCCCACGACGTCGGCCCGCGCGCGATGAGTCCGAAGGTCA
AGGGCTTCGTGCAGCCCAAGAGCTGGTTCGTCGACTTCTCGCCGGTGTCGATGGCGCCGTAA

Upstream 100 bases:

>100_bases
GATCGGTCGAATTATATGCATACGAATGAGATTGCCGGTTTGAGTGGCACGGGCATTGCATAATCCAGCGTACAAATTAG
TCACACGGGAAGTTTCCACC

Downstream 100 bases:

>100_bases
ATCACCACTGCAGAAACCCTGCCCCGTCGTTGCGAGGAGCGAAGCGACGAAGCAATCCAGCCCAGTACGCTGATCACTGG
ATTGCTTCGCTGCGCTCGCA

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Hemin-binding lipoprotein [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MRHLHSFSRTAVLLALTAVGSVAVPQIASSETVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDGLTMWDLSSATKAS
VVIPGLATEWKVEDADKTKWIFKLRPGVTFHDGTPFNADAVVWNVDKVLNKEAVQFDASQVGVTASRMPTLVSAKKIDDM
TVELTAKEPDSFLPINLTNLFIVSPSKWQALYEKAEGADAKARSQAAWAAFAKDAAGTGPWKMAKFTPRERLELVKNDNY
WDKARVPKTDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEITARGFKIEKNEQPHVWPWQFSRIEGSPWNDIRVR
RAANLCIDREGLRDGLLAGLMVPATGTFEPGHPWRGNPSFQIKYDLPAAQKLMKEAGFGPDKKLSVKVQTSASGSGQMLP
LPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGVKDPSANGSNATNVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
NPKFDELVKKARTSFDAAERDKALAELNAASIDDAAFLYVAHDVGPRAMSPKVKGFVQPKSWFVDFSPVSMAP

Sequences:

>Translated_553_residues
MRHLHSFSRTAVLLALTAVGSVAVPQIASSETVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDGLTMWDLSSATKAS
VVIPGLATEWKVEDADKTKWIFKLRPGVTFHDGTPFNADAVVWNVDKVLNKEAVQFDASQVGVTASRMPTLVSAKKIDDM
TVELTAKEPDSFLPINLTNLFIVSPSKWQALYEKAEGADAKARSQAAWAAFAKDAAGTGPWKMAKFTPRERLELVKNDNY
WDKARVPKTDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEITARGFKIEKNEQPHVWPWQFSRIEGSPWNDIRVR
RAANLCIDREGLRDGLLAGLMVPATGTFEPGHPWRGNPSFQIKYDLPAAQKLMKEAGFGPDKKLSVKVQTSASGSGQMLP
LPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGVKDPSANGSNATNVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
NPKFDELVKKARTSFDAAERDKALAELNAASIDDAAFLYVAHDVGPRAMSPKVKGFVQPKSWFVDFSPVSMAP
>Mature_553_residues
MRHLHSFSRTAVLLALTAVGSVAVPQIASSETVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDGLTMWDLSSATKAS
VVIPGLATEWKVEDADKTKWIFKLRPGVTFHDGTPFNADAVVWNVDKVLNKEAVQFDASQVGVTASRMPTLVSAKKIDDM
TVELTAKEPDSFLPINLTNLFIVSPSKWQALYEKAEGADAKARSQAAWAAFAKDAAGTGPWKMAKFTPRERLELVKNDNY
WDKARVPKTDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEITARGFKIEKNEQPHVWPWQFSRIEGSPWNDIRVR
RAANLCIDREGLRDGLLAGLMVPATGTFEPGHPWRGNPSFQIKYDLPAAQKLMKEAGFGPDKKLSVKVQTSASGSGQMLP
LPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGVKDPSANGSNATNVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
NPKFDELVKKARTSFDAAERDKALAELNAASIDDAAFLYVAHDVGPRAMSPKVKGFVQPKSWFVDFSPVSMAP

Specific function: Important role in heme acquisition or metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=511, Percent_Identity=25.6360078277886, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI87081878, Length=568, Percent_Identity=22.3591549295775, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787052, Length=474, Percent_Identity=24.0506329113924, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787495, Length=563, Percent_Identity=22.9129662522202, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61014; Mature: 61014

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLHSFSRTAVLLALTAVGSVAVPQIASSETVLRIGMTAADIPRTLGQPDQGFEGNRFT
CCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCEEE
GLTMYDGLTMWDLSSATKASVVIPGLATEWKVEDADKTKWIFKLRPGVTFHDGTPFNADA
EEEEECCEEEEECCCCCCCEEEECCCCCCEEECCCCCCEEEEEECCCEEECCCCCCCCCE
VVWNVDKVLNKEAVQFDASQVGVTASRMPTLVSAKKIDDMTVELTAKEPDSFLPINLTNL
EEEEHHHHHCHHHHEECHHHHCCCHHHCCHHHHHHCCCCEEEEEEECCCCCEEEEEEEEE
FIVSPSKWQALYEKAEGADAKARSQAAWAAFAKDAAGTGPWKMAKFTPRERLELVKNDNY
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCCC
WDKARVPKTDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEITARGFKIEKNEQPH
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEECCCCCHHHHHHHHCCEEEECCCCCC
VWPWQFSRIEGSPWNDIRVRRAANLCIDREGLRDGLLAGLMVPATGTFEPGHPWRGNPSF
CCCCEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCE
QIKYDLPAAQKLMKEAGFGPDKKLSVKVQTSASGSGQMLPLPMNEYLQQALAECYFDVKL
EEEECCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHEEEEEE
DVIEWNTLFTNWRRGVKDPSANGSNATNVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
EEEECHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NPKFDELVKKARTSFDAAERDKALAELNAASIDDAAFLYVAHDVGPRAMSPKVKGFVQPK
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCC
SWFVDFSPVSMAP
CEEEECCCCCCCC
>Mature Secondary Structure
MRHLHSFSRTAVLLALTAVGSVAVPQIASSETVLRIGMTAADIPRTLGQPDQGFEGNRFT
CCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCEEE
GLTMYDGLTMWDLSSATKASVVIPGLATEWKVEDADKTKWIFKLRPGVTFHDGTPFNADA
EEEEECCEEEEECCCCCCCEEEECCCCCCEEECCCCCCEEEEEECCCEEECCCCCCCCCE
VVWNVDKVLNKEAVQFDASQVGVTASRMPTLVSAKKIDDMTVELTAKEPDSFLPINLTNL
EEEEHHHHHCHHHHEECHHHHCCCHHHCCHHHHHHCCCCEEEEEEECCCCCEEEEEEEEE
FIVSPSKWQALYEKAEGADAKARSQAAWAAFAKDAAGTGPWKMAKFTPRERLELVKNDNY
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCCC
WDKARVPKTDRMVLLPMPEANARTAALLSGQVDWIEAPAPDAVKEITARGFKIEKNEQPH
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEECCCCCHHHHHHHHCCEEEECCCCCC
VWPWQFSRIEGSPWNDIRVRRAANLCIDREGLRDGLLAGLMVPATGTFEPGHPWRGNPSF
CCCCEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCE
QIKYDLPAAQKLMKEAGFGPDKKLSVKVQTSASGSGQMLPLPMNEYLQQALAECYFDVKL
EEEECCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHEEEEEE
DVIEWNTLFTNWRRGVKDPSANGSNATNVTYAAMDPFFALVRFLQSSMAPPVSNNWGYIN
EEEECHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NPKFDELVKKARTSFDAAERDKALAELNAASIDDAAFLYVAHDVGPRAMSPKVKGFVQPK
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCC
SWFVDFSPVSMAP
CEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339409; 7542800; 2041470 [H]