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The map label for this gene is gsiD [H]

Identifier: 86747349

GI number: 86747349

Start: 251104

End: 251991

Strand: Reverse

Name: gsiD [H]

Synonym: RPB_0223

Alternate gene names: 86747349

Gene position: 251991-251104 (Counterclockwise)

Preceding gene: 86747350

Following gene: 86747348

Centisome position: 4.73

GC content: 66.44

Gene sequence:

>888_bases
ATGACCGCGATCACCGAAACCGCCGTGCAGACCGCGCCCGCCGGCAACGCGCGCGGCTACTGGTCCACCGTCGGCCGCAG
GCTCTCGCGCGACAAGGTCAGCATCGCCTGCGCGATCGTCCTGCTGCTGATCCTGCTGTCGGCGATCTTCGCGCCGTATC
TGCATCTCGCCGATCCGTATCAGGGCTCGATGATCCGAAGGCTGAAGCCGATCGGCACGCCGAACTATCCGCTCGGCAGC
GACGAACTCGGCCGCGACATGCTGGCGCGGCTGATCTATGGCGGGCGGCTGTCGCTGCTGATTGGCATTCTTCCCGTCGT
GCTGGCCTTCGTGCTAGGCACTTCGCTGGGCCTCGTCGCCGGCTATGTCGGCGGCAAGGTCAACACCGCGATCATGCGCA
CCATCGACGTGTTCTACGCGTTTCCGTCGGTGCTGCTGGCGATCGCGATCTCGGGCGCGCTCGGCGCCGGCATCACCAAT
TCGATCGTGTCGCTGACCGTGGTGTTCCTGCCGCAGATCACCCGCGTCGCCGAAAGCGTCACCACCGGCGTTCGCAACAT
GGATTTCGTCGAGGCCGCGCGCGCCTCCGGCGCCGGGCCGTTCACCATCATGCGCGTGCACATGCTCGGCAACGTGCTGG
GGCCGATCTTCGTCTACGCCACCGGGCTGATCTCGGTGTCGATGATCCTCGCCGCCGGCCTGTCGTTCCTGGGCCTCGGC
ACCAAGCCGCCGGAGCCCGAATGGGGGCTGATGCTGAACACGCTGCGCACCGCGATCTACGTCAATCCCTGGGTGGCGGC
GCTGCCCGGCGCGATGATCTTCGCGGTCTCGATCTGCTTCAACCTGCTGAGCGACGGCTTGCGCAGCGCCATGGATATCA
GGAACTGA

Upstream 100 bases:

>100_bases
CTGGCGCTGTTCTTCGTGTTCCTCAATCTGGTGGTGGACATCGCGCAGGCCGCGATCGACCCGCGCATCAAGCGCGCCTG
AGCAGGAAGGATTGCCGCTC

Downstream 100 bases:

>100_bases
GATGACGCAAGCCGCAGAACTCAGCCACGGCCTCGAGCCGATCGAGGATATCGGCGGCGCCGCGCAGCCGCTGCTGGACG
TCAGAGGTCTCACCAAGCAC

Product: binding-protein dependent transport system inner membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTAITETAVQTAPAGNARGYWSTVGRRLSRDKVSIACAIVLLLILLSAIFAPYLHLADPYQGSMIRRLKPIGTPNYPLGS
DELGRDMLARLIYGGRLSLLIGILPVVLAFVLGTSLGLVAGYVGGKVNTAIMRTIDVFYAFPSVLLAIAISGALGAGITN
SIVSLTVVFLPQITRVAESVTTGVRNMDFVEAARASGAGPFTIMRVHMLGNVLGPIFVYATGLISVSMILAAGLSFLGLG
TKPPEPEWGLMLNTLRTAIYVNPWVAALPGAMIFAVSICFNLLSDGLRSAMDIRN

Sequences:

>Translated_295_residues
MTAITETAVQTAPAGNARGYWSTVGRRLSRDKVSIACAIVLLLILLSAIFAPYLHLADPYQGSMIRRLKPIGTPNYPLGS
DELGRDMLARLIYGGRLSLLIGILPVVLAFVLGTSLGLVAGYVGGKVNTAIMRTIDVFYAFPSVLLAIAISGALGAGITN
SIVSLTVVFLPQITRVAESVTTGVRNMDFVEAARASGAGPFTIMRVHMLGNVLGPIFVYATGLISVSMILAAGLSFLGLG
TKPPEPEWGLMLNTLRTAIYVNPWVAALPGAMIFAVSICFNLLSDGLRSAMDIRN
>Mature_294_residues
TAITETAVQTAPAGNARGYWSTVGRRLSRDKVSIACAIVLLLILLSAIFAPYLHLADPYQGSMIRRLKPIGTPNYPLGSD
ELGRDMLARLIYGGRLSLLIGILPVVLAFVLGTSLGLVAGYVGGKVNTAIMRTIDVFYAFPSVLLAIAISGALGAGITNS
IVSLTVVFLPQITRVAESVTTGVRNMDFVEAARASGAGPFTIMRVHMLGNVLGPIFVYATGLISVSMILAAGLSFLGLGT
KPPEPEWGLMLNTLRTAIYVNPWVAALPGAMIFAVSICFNLLSDGLRSAMDIRN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=277, Percent_Identity=38.2671480144404, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787760, Length=289, Percent_Identity=32.1799307958478, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789964, Length=301, Percent_Identity=36.8770764119601, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1787498, Length=282, Percent_Identity=32.9787234042553, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789889, Length=246, Percent_Identity=36.1788617886179, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787549, Length=290, Percent_Identity=30.3448275862069, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1788505, Length=232, Percent_Identity=29.3103448275862, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31183; Mature: 31052

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITETAVQTAPAGNARGYWSTVGRRLSRDKVSIACAIVLLLILLSAIFAPYLHLADPY
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QGSMIRRLKPIGTPNYPLGSDELGRDMLARLIYGGRLSLLIGILPVVLAFVLGTSLGLVA
CHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
GYVGGKVNTAIMRTIDVFYAFPSVLLAIAISGALGAGITNSIVSLTVVFLPQITRVAESV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TTGVRNMDFVEAARASGAGPFTIMRVHMLGNVLGPIFVYATGLISVSMILAAGLSFLGLG
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKPPEPEWGLMLNTLRTAIYVNPWVAALPGAMIFAVSICFNLLSDGLRSAMDIRN
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAITETAVQTAPAGNARGYWSTVGRRLSRDKVSIACAIVLLLILLSAIFAPYLHLADPY
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QGSMIRRLKPIGTPNYPLGSDELGRDMLARLIYGGRLSLLIGILPVVLAFVLGTSLGLVA
CHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
GYVGGKVNTAIMRTIDVFYAFPSVLLAIAISGALGAGITNSIVSLTVVFLPQITRVAESV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TTGVRNMDFVEAARASGAGPFTIMRVHMLGNVLGPIFVYATGLISVSMILAAGLSFLGLG
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKPPEPEWGLMLNTLRTAIYVNPWVAALPGAMIFAVSICFNLLSDGLRSAMDIRN
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA