Click here to switch to the map view.

The map label for this gene is fixJ [H]

Identifier: 86747338

GI number: 86747338

Start: 238067

End: 238480

Strand: Reverse

Name: fixJ [H]

Synonym: RPB_0212

Alternate gene names: 86747338

Gene position: 238480-238067 (Counterclockwise)

Preceding gene: 86747339

Following gene: 86747337

Centisome position: 4.47

GC content: 64.73

Gene sequence:

>414_bases
TTGCTGCACCTATCCACCATCACCATTGTGGACGACGACGAAGACGTTCGTGAGAGCATCTCGAGCTTCTTTCGTTCGGT
CGGGCTGCATGTCGAGGGCTTCGCCGCGGCCGAGACCTTCCTGGCCTCGCCCTCGCTCCCCGACACCGATTGCCTCATTA
CGGATCTGCACATGCCCGGAATGGACGGGCTCGGACTGCACCGCGAGCTCCTGCGGCGCGGCGTCAATATCCCGGTGATC
ATGATGACGGCCTACTCCACCCCGGAGGCACGCGCCGAGGCCAGCGACCTCGGCGCCGCCGGCTTCGTCGATAAACCGGT
CGATCCGGAAATCCTTCTGCACGACGTCGAGGCGATCCTGCGGGCCGCACGGCATCGCCGCGTCCCGTCCGACCGATCAG
GTCCAAAGTCATGA

Upstream 100 bases:

>100_bases
GGGCGCGCGTCGTCTCATATCTTGATATGATGGGCAGCGGCCGGCGGATTCGTCATACCATCGAGACAGCCGGACAAGGC
TATCCCGGTGGGAGACGACG

Downstream 100 bases:

>100_bases
GCAAATCTCCGAACCTCTCGCAATCGCCGCTGATCTGCGTTGTCGACGACGATGAGGATGTCCGTAGTTCGCTCAGCAGC
CTGCTACGGTCGATCGGTTA

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MLHLSTITIVDDDEDVRESISSFFRSVGLHVEGFAAAETFLASPSLPDTDCLITDLHMPGMDGLGLHRELLRRGVNIPVI
MMTAYSTPEARAEASDLGAAGFVDKPVDPEILLHDVEAILRAARHRRVPSDRSGPKS

Sequences:

>Translated_137_residues
MLHLSTITIVDDDEDVRESISSFFRSVGLHVEGFAAAETFLASPSLPDTDCLITDLHMPGMDGLGLHRELLRRGVNIPVI
MMTAYSTPEARAEASDLGAAGFVDKPVDPEILLHDVEAILRAARHRRVPSDRSGPKS
>Mature_137_residues
MLHLSTITIVDDDEDVRESISSFFRSVGLHVEGFAAAETFLASPSLPDTDCLITDLHMPGMDGLGLHRELLRRGVNIPVI
MMTAYSTPEARAEASDLGAAGFVDKPVDPEILLHDVEAILRAARHRRVPSDRSGPKS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=102, Percent_Identity=34.3137254901961, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=34.2592592592593, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 14904; Mature: 14904

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLSTITIVDDDEDVRESISSFFRSVGLHVEGFAAAETFLASPSLPDTDCLITDLHMPG
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCC
MDGLGLHRELLRRGVNIPVIMMTAYSTPEARAEASDLGAAGFVDKPVDPEILLHDVEAIL
CCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RAARHRRVPSDRSGPKS
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLHLSTITIVDDDEDVRESISSFFRSVGLHVEGFAAAETFLASPSLPDTDCLITDLHMPG
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCC
MDGLGLHRELLRRGVNIPVIMMTAYSTPEARAEASDLGAAGFVDKPVDPEILLHDVEAIL
CCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RAARHRRVPSDRSGPKS
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]