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The map label for this gene is nfdA [H]

Identifier: 86747312

GI number: 86747312

Start: 204850

End: 206727

Strand: Reverse

Name: nfdA [H]

Synonym: RPB_0186

Alternate gene names: 86747312

Gene position: 206727-204850 (Counterclockwise)

Preceding gene: 86747313

Following gene: 86747307

Centisome position: 3.88

GC content: 66.99

Gene sequence:

>1878_bases
ATGACCGACGCGCCGGACCTCATCCTGCATCGCGGGCTGTTCACCACGCTGGATCGCGCCAACCCCACGGCGAGCGCGGT
GGCGATCAAGGGCGGCCGCTTCGCCGCGGTCGGTCACGACCGCGACATCCTGCCGACGGCCGGGCCGTCGACGCGGGTGA
TCGACCTGAAGGGCCGGCGCGTACTGCCCGGGCTGATCGACAACCATCTTCACATCATTCGCGGCGGGCTGAATTTCAAC
ATGGAGCTGCGCTGGGACGGCGTGCGCTCGCTGGCCGACGCGATGAACATGCTCGAGCGCCAGGTCGCAATCACGCCGCC
GCCGCAATGGGTGCGCGTCGTCGGCGGCTTCACCGAACATCAGTTCGTCGAGAAGCGGCTGCCGACCATCGAGGAGCTCA
ACGCGGTGGCGCCGGACACGCCGGTGTTCCTGCTGCATCTGTACGATCGTGCGCTACTCAACGGCGCGGCGCTGCGCGCC
GTCGGCTACACCCGGGACACGCCGGAGCCGCCGGGCGGCGAGATCACCCGCGACGCCAACGGCAATCCGACTGGGCTGCT
GCTGGCGAAGCCGAACGCCAACATCCTGTACGCCACGCTGGCCAAGGGCCCGAAGCTGCCGTTCGACTACCAGGTCAATT
CGACCCGGCATTTCATGCGGGAGCTCAACCGGCTCGGCGTCACCGGCGCGATCGACGCCGGCGGCGGCTTCCAGAACTAT
CCCGACGATTACGCGGTGATCCAGAAGCTCGACGACGAGGGGCTGCTGACGATCCGGCTCGCCTACAACCTGTTCACGCA
GAAGCCGAAGGGCGAGAAGGACGATTTCCTGAACTGGACCAGGACCTCCAAATACAAGCAGGGCAACGACTACTTCCGTC
ACAACGGCGCCGGCGAGATGCTGGTGTTCTCCGCAGCGGACTTCGAGGATTTTCGCGTGCCGCGACCGGACATGCCGGCG
GAGATGGAAGGCGAGCTTGAGGAGGTCGTCCGCGTCCTGGTGCAGAACAAATGGCCGTGGCGGCTGCACGCGACCTATGA
CGAGACGATCTCGCGCGCGCTCGACGTGTTCGAAAAGGTGAATCAGGATACGCCGCTGGAGGGGCTGCACTGGTTCTTCG
ACCACGCCGAGACGATCTCTGACCGCTCGATCGACCGCATCGCGGCGCTCGGCGGTGGCATCGCCGTTCAGCATCGCATG
GCCTATCAGGGCGAGTATTTCGTCGAGCGCTACGGCATCGGCGCGGCGGAGGCGACGCCGCCGGTGAAGAAGATCCTCGA
CAAGGGCGTCAAGGTCTCGGCCGGCACCGACGCGACGCGGGTGGCGTCGTACAATCCGTGGGTGTCGCTGTCGTGGCTGG
TCACCGGTCGCACCGTCGGCGGAATGCGCCTCACGCCGCAGCGCAATTGCCTCGATCGCGAGACCGCGCTGCGGATGTGG
ACCGAGAACGTCACCTGGTTCTCCAACGAAGAAGGCAACAAGGGCCGGATCGCAGTCGGCCAGCTTGCCGATGTCGTGGT
GCCGGACCGCGACTACTTCGCCTGCAGCGAAGCCGAGATCGCCGACACGACATCGTTGCTGACGATGGTCGGTGGCAAGG
TGGTGTACGGCGCCGGCGACTTCGCCAAGCTCGATGACACCGCGCCGCCGCCGGCGATGCCGGACTGGTCGCCGGTGCGG
CGGTTCGGCGGCTATGCGGCCTGGGCGGACGACGAGTCCGGCGCAAAGGTCGCTGCGACGGCGATGCGGATGTGCGGCTG
CGCCAATTCCTGCAACGTCCACGGCCACGACCACGCCACGGCGTGGTCGAGCAAGCTGCCGATCGCCGATCTCAAGAGCT
TCTGGGGAGCGCTCGGCTGCGCCTGCTGGGCGGTGTGA

Upstream 100 bases:

>100_bases
ATCAGGCGAAATGCGCGCCGCACATGTTCGGATCGCTGCCGGGGCGAAGCGACCAGGCGCCGATGCAGACGGCGCGCCGC
AATCCACCGGAGAAGACCTG

Downstream 100 bases:

>100_bases
CAGCCGGGGGGACGCGGCTCGCAGACGATGCAGACGAGAAGAAGCGGAGCCGGGCTCCGCTTCTTCGGCGTGGTGTCGGT
GTTATTCCTTCACGAAAGCG

Product: amidohydrolase-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MTDAPDLILHRGLFTTLDRANPTASAVAIKGGRFAAVGHDRDILPTAGPSTRVIDLKGRRVLPGLIDNHLHIIRGGLNFN
MELRWDGVRSLADAMNMLERQVAITPPPQWVRVVGGFTEHQFVEKRLPTIEELNAVAPDTPVFLLHLYDRALLNGAALRA
VGYTRDTPEPPGGEITRDANGNPTGLLLAKPNANILYATLAKGPKLPFDYQVNSTRHFMRELNRLGVTGAIDAGGGFQNY
PDDYAVIQKLDDEGLLTIRLAYNLFTQKPKGEKDDFLNWTRTSKYKQGNDYFRHNGAGEMLVFSAADFEDFRVPRPDMPA
EMEGELEEVVRVLVQNKWPWRLHATYDETISRALDVFEKVNQDTPLEGLHWFFDHAETISDRSIDRIAALGGGIAVQHRM
AYQGEYFVERYGIGAAEATPPVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGMRLTPQRNCLDRETALRMW
TENVTWFSNEEGNKGRIAVGQLADVVVPDRDYFACSEAEIADTTSLLTMVGGKVVYGAGDFAKLDDTAPPPAMPDWSPVR
RFGGYAAWADDESGAKVAATAMRMCGCANSCNVHGHDHATAWSSKLPIADLKSFWGALGCACWAV

Sequences:

>Translated_625_residues
MTDAPDLILHRGLFTTLDRANPTASAVAIKGGRFAAVGHDRDILPTAGPSTRVIDLKGRRVLPGLIDNHLHIIRGGLNFN
MELRWDGVRSLADAMNMLERQVAITPPPQWVRVVGGFTEHQFVEKRLPTIEELNAVAPDTPVFLLHLYDRALLNGAALRA
VGYTRDTPEPPGGEITRDANGNPTGLLLAKPNANILYATLAKGPKLPFDYQVNSTRHFMRELNRLGVTGAIDAGGGFQNY
PDDYAVIQKLDDEGLLTIRLAYNLFTQKPKGEKDDFLNWTRTSKYKQGNDYFRHNGAGEMLVFSAADFEDFRVPRPDMPA
EMEGELEEVVRVLVQNKWPWRLHATYDETISRALDVFEKVNQDTPLEGLHWFFDHAETISDRSIDRIAALGGGIAVQHRM
AYQGEYFVERYGIGAAEATPPVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGMRLTPQRNCLDRETALRMW
TENVTWFSNEEGNKGRIAVGQLADVVVPDRDYFACSEAEIADTTSLLTMVGGKVVYGAGDFAKLDDTAPPPAMPDWSPVR
RFGGYAAWADDESGAKVAATAMRMCGCANSCNVHGHDHATAWSSKLPIADLKSFWGALGCACWAV
>Mature_624_residues
TDAPDLILHRGLFTTLDRANPTASAVAIKGGRFAAVGHDRDILPTAGPSTRVIDLKGRRVLPGLIDNHLHIIRGGLNFNM
ELRWDGVRSLADAMNMLERQVAITPPPQWVRVVGGFTEHQFVEKRLPTIEELNAVAPDTPVFLLHLYDRALLNGAALRAV
GYTRDTPEPPGGEITRDANGNPTGLLLAKPNANILYATLAKGPKLPFDYQVNSTRHFMRELNRLGVTGAIDAGGGFQNYP
DDYAVIQKLDDEGLLTIRLAYNLFTQKPKGEKDDFLNWTRTSKYKQGNDYFRHNGAGEMLVFSAADFEDFRVPRPDMPAE
MEGELEEVVRVLVQNKWPWRLHATYDETISRALDVFEKVNQDTPLEGLHWFFDHAETISDRSIDRIAALGGGIAVQHRMA
YQGEYFVERYGIGAAEATPPVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGMRLTPQRNCLDRETALRMWT
ENVTWFSNEEGNKGRIAVGQLADVVVPDRDYFACSEAEIADTTSLLTMVGGKVVYGAGDFAKLDDTAPPPAMPDWSPVRR
FGGYAAWADDESGAKVAATAMRMCGCANSCNVHGHDHATAWSSKLPIADLKSFWGALGCACWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 68885; Mature: 68754

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAPDLILHRGLFTTLDRANPTASAVAIKGGRFAAVGHDRDILPTAGPSTRVIDLKGRR
CCCCHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCEEEEECCCE
VLPGLIDNHLHIIRGGLNFNMELRWDGVRSLADAMNMLERQVAITPPPQWVRVVGGFTEH
ECCHHHCCCEEEEECCCEEEEEEEECHHHHHHHHHHHHHHCEECCCCHHHHHHHCCCHHH
QFVEKRLPTIEELNAVAPDTPVFLLHLYDRALLNGAALRAVGYTRDTPEPPGGEITRDAN
HHHHHHCCCHHHHHCCCCCCCEEHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCEEECCC
GNPTGLLLAKPNANILYATLAKGPKLPFDYQVNSTRHFMRELNRLGVTGAIDAGGGFQNY
CCCCEEEEECCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHCCCCEEEECCCCCCCC
PDDYAVIQKLDDEGLLTIRLAYNLFTQKPKGEKDDFLNWTRTSKYKQGNDYFRHNGAGEM
CCHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCHHHCCCHHCCCCCCCCCHHEECCCCCE
LVFSAADFEDFRVPRPDMPAEMEGELEEVVRVLVQNKWPWRLHATYDETISRALDVFEKV
EEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHC
NQDTPLEGLHWFFDHAETISDRSIDRIAALGGGIAVQHRMAYQGEYFVERYGIGAAEATP
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCCCCC
PVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGMRLTPQRNCLDRETALRMW
HHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCEECCEEECCCCCCCCHHHHHHHH
TENVTWFSNEEGNKGRIAVGQLADVVVPDRDYFACSEAEIADTTSLLTMVGGKVVYGAGD
HCCCEEEECCCCCCCCEECCCHHEEEECCCCCEECCCCCHHHHHHHHHHHCCEEEECCCC
FAKLDDTAPPPAMPDWSPVRRFGGYAAWADDESGAKVAATAMRMCGCANSCNVHGHDHAT
CCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
AWSSKLPIADLKSFWGALGCACWAV
CCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDAPDLILHRGLFTTLDRANPTASAVAIKGGRFAAVGHDRDILPTAGPSTRVIDLKGRR
CCCHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCEEEEECCCE
VLPGLIDNHLHIIRGGLNFNMELRWDGVRSLADAMNMLERQVAITPPPQWVRVVGGFTEH
ECCHHHCCCEEEEECCCEEEEEEEECHHHHHHHHHHHHHHCEECCCCHHHHHHHCCCHHH
QFVEKRLPTIEELNAVAPDTPVFLLHLYDRALLNGAALRAVGYTRDTPEPPGGEITRDAN
HHHHHHCCCHHHHHCCCCCCCEEHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCEEECCC
GNPTGLLLAKPNANILYATLAKGPKLPFDYQVNSTRHFMRELNRLGVTGAIDAGGGFQNY
CCCCEEEEECCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHCCCCEEEECCCCCCCC
PDDYAVIQKLDDEGLLTIRLAYNLFTQKPKGEKDDFLNWTRTSKYKQGNDYFRHNGAGEM
CCHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCHHHCCCHHCCCCCCCCCHHEECCCCCE
LVFSAADFEDFRVPRPDMPAEMEGELEEVVRVLVQNKWPWRLHATYDETISRALDVFEKV
EEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHC
NQDTPLEGLHWFFDHAETISDRSIDRIAALGGGIAVQHRMAYQGEYFVERYGIGAAEATP
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCCCCC
PVKKILDKGVKVSAGTDATRVASYNPWVSLSWLVTGRTVGGMRLTPQRNCLDRETALRMW
HHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCEECCEEECCCCCCCCHHHHHHHH
TENVTWFSNEEGNKGRIAVGQLADVVVPDRDYFACSEAEIADTTSLLTMVGGKVVYGAGD
HCCCEEEECCCCCCCCEECCCHHEEEECCCCCEECCCCCHHHHHHHHHHHCCEEEECCCC
FAKLDDTAPPPAMPDWSPVRRFGGYAAWADDESGAKVAATAMRMCGCANSCNVHGHDHAT
CCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
AWSSKLPIADLKSFWGALGCACWAV
CCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA