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The map label for this gene is rlmN

Identifier: 86747220

GI number: 86747220

Start: 99721

End: 100920

Strand: Reverse

Name: rlmN

Synonym: RPB_0093

Alternate gene names: 86747220

Gene position: 100920-99721 (Counterclockwise)

Preceding gene: 86747221

Following gene: 86747219

Centisome position: 1.89

GC content: 65.25

Gene sequence:

>1200_bases
ATGACCCTCGCTGTTGCCGTCGCACCGCTCGAAAAGACCCCGCTGGAGACCTATGTGCCGCCGGCCAAGCCGTCGCTGAT
CGGGCTGTCGCGCGCCGAACTGGCCGAACGGCTCGGCGCGATCGGCGTGGCGCCGGCGCAGCGCAAGATGCGGGCGCAGC
AATTGTGGCACTGGATGTATGTCCGCGGCGCCCGCGATTTCGCCGAGATGACCAACGTCTCCAAGGAGATGCGAGCGACG
CTGGCGGAGCATTGCACGGTCGACCGGCCCGAGGTCGTCGCCGAGCAGATCTCCGCCGACGGCACCCGCAAATGGCTGCT
GCGGCTGCCGAGTGGCGACGATGTGCAGAAGGCGCACGAGGTCGAGTGCGTCTACATCCCGGAGACCGACCGCGGCACGC
TGTGCGTGTCGAGCCAGGTCGGCTGCACGCTGAACTGCTCGTTCTGCCACACCGGCACCCAGCGGCTGGTGCGCAACCTC
ACCGCCGGCGAGATCGTCGGTCAGGTCATGGTGGCGCGGGATCGCCTGGGCGACTGGATCGATCGCGAGACCCCCAATGG
CAACCGTCTCGTCACCAACGTGGTGATGATGGGCATGGGCGAGCCGTTGTACAATTTCGAGGCGGTGCGCGACGCGCTCC
TGATCGTCACCGACAATGAAGGCATCGGTATCTCGCGGCGGCGGGTGACGCTGTCGACCTCCGGTGTGGTGCCGAACATC
GCCCGCACCGGCGACGAGATCGGCGTGATGCTGGCGATTTCGCTGCACGCGGTGCGCGACGAATTGCGCGACGAACTGGT
GCCGCTGAACCGCAAATATCCACTCAAAGAGCTGCTGCAGGCCTGCCGCGACTATCCGGGCGCCTCGAATGCGCGCCGTA
TCACCTTCGAATATGTGATGCTGAAGGGCGTCAACGATTCGCTCGACGATGCGCGGCGGCTGGTGCAGTTGCTGAAGGGC
ATTCCGGCCAAGATCAATCTGATCCCGTTCAACCCCTGGCCCGGCTCGAAATACGAGTGCTCGGATTGGGACCAGATCGA
GAAGTTCTCGGAGTATGTCTTCAACGCCGGCTATTCCTCGCCGGTCCGTACCCCGCGCGGCCGCGATATTCTCGCCGCCT
GCGGACAGTTGAAGTCGGAGACCGAAAAGCTCTCGGTCCGCGAACGCGACGCGCTCCGCGCGATGGCGATGACGGATTGA

Upstream 100 bases:

>100_bases
CGACGTGCCCTGCGCTTCCTCCTTCGAGACGCGGCCTATGGCCGCTCCTCAGGATGAGGCCCGTTCTGCCGCGATTGCCT
TTTGCCTGAACCGGACCGCC

Downstream 100 bases:

>100_bases
TGGGCAAGATCGCTGCCGAACGTCATCCTGAGGTGCGCGGCGCGCTTGCGGCGCGCCTCGAAGGATGTGCGCACGAAGTC
ATCCTTCGAGGCTCGCAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTLAVAVAPLEKTPLETYVPPAKPSLIGLSRAELAERLGAIGVAPAQRKMRAQQLWHWMYVRGARDFAEMTNVSKEMRAT
LAEHCTVDRPEVVAEQISADGTRKWLLRLPSGDDVQKAHEVECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNL
TAGEIVGQVMVARDRLGDWIDRETPNGNRLVTNVVMMGMGEPLYNFEAVRDALLIVTDNEGIGISRRRVTLSTSGVVPNI
ARTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARRLVQLLKG
IPAKINLIPFNPWPGSKYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVRERDALRAMAMTD

Sequences:

>Translated_399_residues
MTLAVAVAPLEKTPLETYVPPAKPSLIGLSRAELAERLGAIGVAPAQRKMRAQQLWHWMYVRGARDFAEMTNVSKEMRAT
LAEHCTVDRPEVVAEQISADGTRKWLLRLPSGDDVQKAHEVECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNL
TAGEIVGQVMVARDRLGDWIDRETPNGNRLVTNVVMMGMGEPLYNFEAVRDALLIVTDNEGIGISRRRVTLSTSGVVPNI
ARTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARRLVQLLKG
IPAKINLIPFNPWPGSKYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVRERDALRAMAMTD
>Mature_398_residues
TLAVAVAPLEKTPLETYVPPAKPSLIGLSRAELAERLGAIGVAPAQRKMRAQQLWHWMYVRGARDFAEMTNVSKEMRATL
AEHCTVDRPEVVAEQISADGTRKWLLRLPSGDDVQKAHEVECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNLT
AGEIVGQVMVARDRLGDWIDRETPNGNRLVTNVVMMGMGEPLYNFEAVRDALLIVTDNEGIGISRRRVTLSTSGVVPNIA
RTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARRLVQLLKGI
PAKINLIPFNPWPGSKYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVRERDALRAMAMTD

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=382, Percent_Identity=47.3821989528796, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_RHOP2 (Q2J405)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483716.1
- ProteinModelPortal:   Q2J405
- STRING:   Q2J405
- GeneID:   3908735
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0093
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK910499
- BioCyc:   RPAL316058:RPB_0093-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 44429; Mature: 44297

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAVAVAPLEKTPLETYVPPAKPSLIGLSRAELAERLGAIGVAPAQRKMRAQQLWHWMY
CEEEEEECCCCCCCHHHCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VRGARDFAEMTNVSKEMRATLAEHCTVDRPEVVAEQISADGTRKWLLRLPSGDDVQKAHE
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHC
VECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNLTAGEIVGQVMVARDRLGDWI
CEEEEECCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DRETPNGNRLVTNVVMMGMGEPLYNFEAVRDALLIVTDNEGIGISRRRVTLSTSGVVPNI
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCCCEEEEEEEECCCCCCCH
ARTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVM
HHCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
LKGVNDSLDDARRLVQLLKGIPAKINLIPFNPWPGSKYECSDWDQIEKFSEYVFNAGYSS
EECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PVRTPRGRDILAACGQLKSETEKLSVRERDALRAMAMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAVAVAPLEKTPLETYVPPAKPSLIGLSRAELAERLGAIGVAPAQRKMRAQQLWHWMY
EEEEEECCCCCCCHHHCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VRGARDFAEMTNVSKEMRATLAEHCTVDRPEVVAEQISADGTRKWLLRLPSGDDVQKAHE
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHC
VECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNLTAGEIVGQVMVARDRLGDWI
CEEEEECCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DRETPNGNRLVTNVVMMGMGEPLYNFEAVRDALLIVTDNEGIGISRRRVTLSTSGVVPNI
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCCCEEEEEEEECCCCCCCH
ARTGDEIGVMLAISLHAVRDELRDELVPLNRKYPLKELLQACRDYPGASNARRITFEYVM
HHCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
LKGVNDSLDDARRLVQLLKGIPAKINLIPFNPWPGSKYECSDWDQIEKFSEYVFNAGYSS
EECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PVRTPRGRDILAACGQLKSETEKLSVRERDALRAMAMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA