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The map label for this gene is phoA [H]

Identifier: 86747216

GI number: 86747216

Start: 94763

End: 96517

Strand: Reverse

Name: phoA [H]

Synonym: RPB_0089

Alternate gene names: 86747216

Gene position: 96517-94763 (Counterclockwise)

Preceding gene: 162138526

Following gene: 86747213

Centisome position: 1.81

GC content: 67.98

Gene sequence:

>1755_bases
ATGATGCGTTTCGCCACGACCTTTTTTGCCTTGCTGGTGTCGACTTCGTTCGCCGCGGCGCAGACGATCTATCCGATCGA
CCGCGCCGAAATTCTGGCCGGCGCCCGGTTCGATCTCAAGGTCGAATTCGCCGGCGAAGTCTCCGCCGACAAGGCGACGC
TGACCGTCAACGGCACGGATGCCGCAGCGGTGTTCGGCAAGGCTCCGGAGCTGATCGCGCGCGAGGACGGCCGACCGCAA
TCGGCGCTGCTGCTGCGCGACGTCGCGCTCGACAAACCCGGCGCCTACACGGTCGAGGCCGGCGACGGCGCGGACCGCCG
CAGCGTCGGCTGGACGGTGTACGACACTGGGCCGCGCAAGGCCAAGAACGTCATCCTGTTCATCGGCGACGGGCTGTCGC
CCGCGCATCGCGTCGCTGCGCGATTGCTGTCGAAGGGCATCAAGGAAGGCCGCGCCGGCGGCAAGCTGGCGATCGACGAC
ATGCCGCAGATGGCGCTGGTGTCGACCGCCGGCAGCGATTCGATCATCACCGATTCCGCCAATGCGGCGAGCGCCTACGC
CACCGGCCACAAGGCCGCGGTCAATGCGATGGGCGTCTATGCCGACCGCACCGCGAGCCCGCTCGACGATCCCAGGGTCG
AGACGCTGGCGTCGCTGGCGAAGCGGCGGCTGGGACTTGCGATCGGCATCGTCACCAATACCGAGGTGGAAGACGCCACG
CCCGCGGCGGTGATCGCCCATACCCGCCGTCGCGCCGCGTATGACGACATCGTCGCGCAGTTCTTTGCCGCAAGTCCCGA
CGTGCTGATGGGCGGCGGCGCCGCGTATTTCCTGCCGAAGAGCGCGCAGGGCAAGCGCAAGGACGACGTCGACTATCTGG
CGAAGTTCCGCGACGCCGGCTACGCGGTCGCCACCAGTGCGACCGAGCTGGCCGCGGCCGGCAAGCCCGACACGCGAAAA
CTGCTCGGCCTGTTCGCGCCCGGCAACATGGACGGCGTGCTCGATCGCCGCTTCCTGAAAGGCGGCGGCGTCGCGAAGAA
TCCGGATCAGCCCGATCTGACCGAGCAGGTCGCGGTCGCGCTCGACGTCCTGTCGAAGAACGAGGCCGGCTTCTTCTTGA
TGGTCGAATCCGGGATGATCGACAAATACGCCCACGCGCTCGACATGGAGCGCGCGGTCTACGACACCATCATGCTCGAC
AATGCGGTGCGGCAAACGCGCGACTGGGCCAAGGCACGCGGCGACGACACGCTGATCCTGGTGGTCGCCGATCACAACCA
TCCCAACGCGCTGGTCGGCACCGTGCGCGACGATCTCGCCGAGGGCGAGGACGTGCCGCTGCGCGAACGCATCGGCGTGT
ATCAGAGAGCCGGCTTTCCGAACTACCCGGCGCCCGACAAGGACGGCTATCCGGATCGCGTCGACGTCAGTCGCCGCCTG
GCGATCTTCTCGGCCAGCCTGCCCGATCACTACGAGACGCTGCGGCCGAAGCTCGACGGCCCGAACCAGCCGACCGAGGC
CGGCGACAAGCCCGGCACCTTCAAGGCCAACGAGAAGTACAAGGCCGTGCCGGGGGCGGTTTTGCGGCTCGGCAATCTGC
CGGCGATGATGAACGCCAGCGTGCATTCCGGCGAGGACGTGATCCTCACCGCGGCGGGGCCGGGCAGCGACCGGGTCCAC
GGCGCGATGGAAAACACCGACGTGTTCCGGGTCATGGCCGAGGCGCTCGGGCTCGCGGCGGCCTCGGCGAAATAG

Upstream 100 bases:

>100_bases
GATCAGGCGCGCGTCCCGGAATGACTGCCGCTGCTAAACACCGTCATCGAACCGTCATTTGCCGCTCATACGGTCCGCGC
TCCGATTTGGAGCCGTTCCC

Downstream 100 bases:

>100_bases
AGCGTTTTCGAGCGAAGTGGAAACCGGTTCGCGTGAAGAAAACGCGTCAAAACAAGAATCTGGAGTACCGATTCTGATTC
TATCAGAACCGGAATTGCTC

Product: alkaline phosphatase

Products: NA

Alternate protein names: High molecular weight phosphatase; H-AP [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MMRFATTFFALLVSTSFAAAQTIYPIDRAEILAGARFDLKVEFAGEVSADKATLTVNGTDAAAVFGKAPELIAREDGRPQ
SALLLRDVALDKPGAYTVEAGDGADRRSVGWTVYDTGPRKAKNVILFIGDGLSPAHRVAARLLSKGIKEGRAGGKLAIDD
MPQMALVSTAGSDSIITDSANAASAYATGHKAAVNAMGVYADRTASPLDDPRVETLASLAKRRLGLAIGIVTNTEVEDAT
PAAVIAHTRRRAAYDDIVAQFFAASPDVLMGGGAAYFLPKSAQGKRKDDVDYLAKFRDAGYAVATSATELAAAGKPDTRK
LLGLFAPGNMDGVLDRRFLKGGGVAKNPDQPDLTEQVAVALDVLSKNEAGFFLMVESGMIDKYAHALDMERAVYDTIMLD
NAVRQTRDWAKARGDDTLILVVADHNHPNALVGTVRDDLAEGEDVPLRERIGVYQRAGFPNYPAPDKDGYPDRVDVSRRL
AIFSASLPDHYETLRPKLDGPNQPTEAGDKPGTFKANEKYKAVPGAVLRLGNLPAMMNASVHSGEDVILTAAGPGSDRVH
GAMENTDVFRVMAEALGLAAASAK

Sequences:

>Translated_584_residues
MMRFATTFFALLVSTSFAAAQTIYPIDRAEILAGARFDLKVEFAGEVSADKATLTVNGTDAAAVFGKAPELIAREDGRPQ
SALLLRDVALDKPGAYTVEAGDGADRRSVGWTVYDTGPRKAKNVILFIGDGLSPAHRVAARLLSKGIKEGRAGGKLAIDD
MPQMALVSTAGSDSIITDSANAASAYATGHKAAVNAMGVYADRTASPLDDPRVETLASLAKRRLGLAIGIVTNTEVEDAT
PAAVIAHTRRRAAYDDIVAQFFAASPDVLMGGGAAYFLPKSAQGKRKDDVDYLAKFRDAGYAVATSATELAAAGKPDTRK
LLGLFAPGNMDGVLDRRFLKGGGVAKNPDQPDLTEQVAVALDVLSKNEAGFFLMVESGMIDKYAHALDMERAVYDTIMLD
NAVRQTRDWAKARGDDTLILVVADHNHPNALVGTVRDDLAEGEDVPLRERIGVYQRAGFPNYPAPDKDGYPDRVDVSRRL
AIFSASLPDHYETLRPKLDGPNQPTEAGDKPGTFKANEKYKAVPGAVLRLGNLPAMMNASVHSGEDVILTAAGPGSDRVH
GAMENTDVFRVMAEALGLAAASAK
>Mature_584_residues
MMRFATTFFALLVSTSFAAAQTIYPIDRAEILAGARFDLKVEFAGEVSADKATLTVNGTDAAAVFGKAPELIAREDGRPQ
SALLLRDVALDKPGAYTVEAGDGADRRSVGWTVYDTGPRKAKNVILFIGDGLSPAHRVAARLLSKGIKEGRAGGKLAIDD
MPQMALVSTAGSDSIITDSANAASAYATGHKAAVNAMGVYADRTASPLDDPRVETLASLAKRRLGLAIGIVTNTEVEDAT
PAAVIAHTRRRAAYDDIVAQFFAASPDVLMGGGAAYFLPKSAQGKRKDDVDYLAKFRDAGYAVATSATELAAAGKPDTRK
LLGLFAPGNMDGVLDRRFLKGGGVAKNPDQPDLTEQVAVALDVLSKNEAGFFLMVESGMIDKYAHALDMERAVYDTIMLD
NAVRQTRDWAKARGDDTLILVVADHNHPNALVGTVRDDLAEGEDVPLRERIGVYQRAGFPNYPAPDKDGYPDRVDVSRRL
AIFSASLPDHYETLRPKLDGPNQPTEAGDKPGTFKANEKYKAVPGAVLRLGNLPAMMNASVHSGEDVILTAAGPGSDRVH
GAMENTDVFRVMAEALGLAAASAK

Specific function: Has only a phosphomonoesterase activity [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted. Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=500, Percent_Identity=29.8, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI157266292, Length=502, Percent_Identity=30.2788844621514, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI157266296, Length=341, Percent_Identity=33.7243401759531, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI94721246, Length=342, Percent_Identity=33.9181286549708, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI294660770, Length=495, Percent_Identity=29.0909090909091, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI294660772, Length=458, Percent_Identity=28.1659388646288, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI48994877, Length=356, Percent_Identity=33.1460674157303, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320689, Length=339, Percent_Identity=30.0884955752212, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI21355981, Length=488, Percent_Identity=28.2786885245902, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21355151, Length=497, Percent_Identity=28.9738430583501, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI85725200, Length=348, Percent_Identity=32.7586206896552, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI85815833, Length=348, Percent_Identity=32.7586206896552, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21358067, Length=429, Percent_Identity=30.5361305361305, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24585213, Length=358, Percent_Identity=32.4022346368715, Blast_Score=131, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24657827, Length=352, Percent_Identity=31.5340909090909, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24657842, Length=493, Percent_Identity=28.1947261663286, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24657835, Length=493, Percent_Identity=27.5862068965517, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859923, Length=476, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21355149, Length=348, Percent_Identity=31.3218390804598, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19921912, Length=497, Percent_Identity=27.5653923541247, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24651554, Length=510, Percent_Identity=25.2941176470588, Blast_Score=106, Evalue=5e-23,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 61946; Mature: 61946

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRFATTFFALLVSTSFAAAQTIYPIDRAEILAGARFDLKVEFAGEVSADKATLTVNGTD
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCC
AAAVFGKAPELIAREDGRPQSALLLRDVALDKPGAYTVEAGDGADRRSVGWTVYDTGPRK
HHHHHCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCC
AKNVILFIGDGLSPAHRVAARLLSKGIKEGRAGGKLAIDDMPQMALVSTAGSDSIITDSA
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCEEECCC
NAASAYATGHKAAVNAMGVYADRTASPLDDPRVETLASLAKRRLGLAIGIVTNTEVEDAT
CCCHHHHCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
PAAVIAHTRRRAAYDDIVAQFFAASPDVLMGGGAAYFLPKSAQGKRKDDVDYLAKFRDAG
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHCCC
YAVATSATELAAAGKPDTRKLLGLFAPGNMDGVLDRRFLKGGGVAKNPDQPDLTEQVAVA
EEEECCHHHHHHCCCCCHHHHEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LDVLSKNEAGFFLMVESGMIDKYAHALDMERAVYDTIMLDNAVRQTRDWAKARGDDTLIL
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VVADHNHPNALVGTVRDDLAEGEDVPLRERIGVYQRAGFPNYPAPDKDGYPDRVDVSRRL
EEECCCCCCCEEEHHHHHHCCCCCCCHHHHHCHHHHCCCCCCCCCCCCCCCCCCCHHHEE
AIFSASLPDHYETLRPKLDGPNQPTEAGDKPGTFKANEKYKAVPGAVLRLGNLPAMMNAS
EEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCCC
VHSGEDVILTAAGPGSDRVHGAMENTDVFRVMAEALGLAAASAK
CCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MMRFATTFFALLVSTSFAAAQTIYPIDRAEILAGARFDLKVEFAGEVSADKATLTVNGTD
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCC
AAAVFGKAPELIAREDGRPQSALLLRDVALDKPGAYTVEAGDGADRRSVGWTVYDTGPRK
HHHHHCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCC
AKNVILFIGDGLSPAHRVAARLLSKGIKEGRAGGKLAIDDMPQMALVSTAGSDSIITDSA
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCEEECCC
NAASAYATGHKAAVNAMGVYADRTASPLDDPRVETLASLAKRRLGLAIGIVTNTEVEDAT
CCCHHHHCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
PAAVIAHTRRRAAYDDIVAQFFAASPDVLMGGGAAYFLPKSAQGKRKDDVDYLAKFRDAG
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHCCC
YAVATSATELAAAGKPDTRKLLGLFAPGNMDGVLDRRFLKGGGVAKNPDQPDLTEQVAVA
EEEECCHHHHHHCCCCCHHHHEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LDVLSKNEAGFFLMVESGMIDKYAHALDMERAVYDTIMLDNAVRQTRDWAKARGDDTLIL
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VVADHNHPNALVGTVRDDLAEGEDVPLRERIGVYQRAGFPNYPAPDKDGYPDRVDVSRRL
EEECCCCCCCEEEHHHHHHCCCCCCCHHHHHCHHHHCCCCCCCCCCCCCCCCCCCHHHEE
AIFSASLPDHYETLRPKLDGPNQPTEAGDKPGTFKANEKYKAVPGAVLRLGNLPAMMNAS
EEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCCC
VHSGEDVILTAAGPGSDRVHGAMENTDVFRVMAEALGLAAASAK
CCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 8454193 [H]