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The map label for this gene is argG [H]

Identifier: 162138526

GI number: 162138526

Start: 96815

End: 98152

Strand: Reverse

Name: argG [H]

Synonym: RPB_0090

Alternate gene names: 162138526

Gene position: 98152-96815 (Counterclockwise)

Preceding gene: 86747218

Following gene: 86747216

Centisome position: 1.84

GC content: 65.1

Gene sequence:

>1338_bases
ATGAGTACGATTCTGAAAAGCCTGCCCAAAGGCGAAAACGTCGGCATCGCGTTTTCGGGCGGGCTCGACACCAGCGCGGC
GCTGCTGTGGATGAAGCAGAAGGGCGCTCGCGTGTTCGCCTATACGGCGAATCTCGGCCAGCCCGACGAGGCCGACTACG
ACGAGATCCCGCGCAAGGCGATGGAGTTTGGCGCCGAGAAGGCCCGGCTGGTCGATTGTCGGTCGCAGCTCGTGCACGAG
GGCATCGCCGCGATCCAGTCGGGCGCCTTCCACGTCTCGACCGGCGGCATCGCCTATTTCAACACCACGCCGCTCGGCCG
CGCCGTCACCGGCACCATGCTGGTCTCGGCGATGAAGGAGGACGGCGTCAATATCTGGGGCGACGGCTCGACCTACAAGG
GCAACGATATCGAGCGGTTCTATCGCTACGGGCTGCTGACCAACCCGGACCTGCGGATCTACAAGCCCTGGCTCGATCAG
CAGTTCATCGACGAGCTCGGCGGCCGCGCCGAAATGTCGGCGTTCATGACCGCCGCCGGCTTCGCCTACAAGATGAGTTC
GGAAAAGGCGTATTCGACCGACAGCAACCTGCTCGGCGCCACCCACGAGGCCAAGGATCTCGAACACCTCGATTCAGGCA
TCAGGATCGTCGTCCCGATCATGGGCGTGCCGTTCTGGCGCGAGGATTGCGCTGTCAAGGCCGAGACCGTCTCGGTGCGG
TTCGAGGAAGGCCAGCCGGTCGCGCTGAACGGCAAGACGTTCGCCGATCCGGTCGCGCTGTTCCTCGAGGCCAATGCGAT
CGGCGGCCGGCACGGGCTCGGCATGAGCGACCAGATCGAGAACCGCATCATCGAGGCCAAGAGCCGCGGCATCTACGAGG
CGCCGGGCATGGCGCTGCTGCACATCGCCTATGAGCGTCTCGTCACCGGCATCCACAACGAAGACACCATCGAGCAGTAT
CGCATCAGCGGCATGAAGCTCGGCCGCCTGCTGTATCAGGGTCGCTGGTTCGACTCGCAGGCCTTGATGCTGCGCGAGAC
GGCGCAGCGCTGGGTGGCGCGCGCCATCACCGGCGAGGTCACGCTCGAGCTGCGCCGCGGCAACGACTACTCGATCCTCA
ACACGCAGAGCCCGAATCTGACCTATGCGCCGGAGCGGCTCAGCATGGAGAAGGTCGAGGACGCGCCGTTCACGCCGGGC
GATCGCATCGGCCAGCTGACGATGCGCAACCTCGATATCGCCGACACCCGCGCCAAGCTGGACATCTTCGCCAAGGCCGG
CCTGCTGTCGGCCGGCGAGGGCTCGCACATCCCGAAGCTCGAAAACGACAAGGGCTGA

Upstream 100 bases:

>100_bases
GCCTTCGACAGACCCGCCCCGATTAAACGCGTGCCCGCCGCCGCGAAGTTTGATATTCGCGCTCCCGACCCGCAAACCGC
CCCAGCCGATTGGAAGTCCC

Downstream 100 bases:

>100_bases
GGCGGCCCACCTTCAACGTCATTCCGGGGCGCACGCAGCGAAGCTGCGGGCGAACCCGGAATCCCGAAGTTCAGGGCACC
TCGCGGTCGGACGCAGAACA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGARVFAYTANLGQPDEADYDEIPRKAMEFGAEKARLVDCRSQLVHE
GIAAIQSGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPDLRIYKPWLDQ
QFIDELGGRAEMSAFMTAAGFAYKMSSEKAYSTDSNLLGATHEAKDLEHLDSGIRIVVPIMGVPFWREDCAVKAETVSVR
FEEGQPVALNGKTFADPVALFLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RISGMKLGRLLYQGRWFDSQALMLRETAQRWVARAITGEVTLELRRGNDYSILNTQSPNLTYAPERLSMEKVEDAPFTPG
DRIGQLTMRNLDIADTRAKLDIFAKAGLLSAGEGSHIPKLENDKG

Sequences:

>Translated_445_residues
MSTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGARVFAYTANLGQPDEADYDEIPRKAMEFGAEKARLVDCRSQLVHE
GIAAIQSGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPDLRIYKPWLDQ
QFIDELGGRAEMSAFMTAAGFAYKMSSEKAYSTDSNLLGATHEAKDLEHLDSGIRIVVPIMGVPFWREDCAVKAETVSVR
FEEGQPVALNGKTFADPVALFLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RISGMKLGRLLYQGRWFDSQALMLRETAQRWVARAITGEVTLELRRGNDYSILNTQSPNLTYAPERLSMEKVEDAPFTPG
DRIGQLTMRNLDIADTRAKLDIFAKAGLLSAGEGSHIPKLENDKG
>Mature_444_residues
STILKSLPKGENVGIAFSGGLDTSAALLWMKQKGARVFAYTANLGQPDEADYDEIPRKAMEFGAEKARLVDCRSQLVHEG
IAAIQSGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPDLRIYKPWLDQQ
FIDELGGRAEMSAFMTAAGFAYKMSSEKAYSTDSNLLGATHEAKDLEHLDSGIRIVVPIMGVPFWREDCAVKAETVSVRF
EEGQPVALNGKTFADPVALFLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYR
ISGMKLGRLLYQGRWFDSQALMLRETAQRWVARAITGEVTLELRRGNDYSILNTQSPNLTYAPERLSMEKVEDAPFTPGD
RIGQLTMRNLDIADTRAKLDIFAKAGLLSAGEGSHIPKLENDKG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=411, Percent_Identity=29.683698296837, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI16950633, Length=411, Percent_Identity=29.683698296837, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1789563, Length=442, Percent_Identity=78.7330316742081, Blast_Score=723, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=29.2565947242206, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21358151, Length=391, Percent_Identity=28.6445012787724, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 48960; Mature: 48829

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGARVFAYTANLGQPDEADYDEIPRKA
CCCHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHH
MEFGAEKARLVDCRSQLVHEGIAAIQSGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKE
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHH
DGVNIWGDGSTYKGNDIERFYRYGLLTNPDLRIYKPWLDQQFIDELGGRAEMSAFMTAAG
CCCEEECCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHCCHHHHHHHHHHHC
FAYKMSSEKAYSTDSNLLGATHEAKDLEHLDSGIRIVVPIMGVPFWREDCAVKAETVSVR
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEEE
FEEGQPVALNGKTFADPVALFLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
ECCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
HIAYERLVTGIHNEDTIEQYRISGMKLGRLLYQGRWFDSQALMLRETAQRWVARAITGEV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEE
TLELRRGNDYSILNTQSPNLTYAPERLSMEKVEDAPFTPGDRIGQLTMRNLDIADTRAKL
EEEEECCCCEEEEECCCCCCEECHHHCCHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHE
DIFAKAGLLSAGEGSHIPKLENDKG
EEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STILKSLPKGENVGIAFSGGLDTSAALLWMKQKGARVFAYTANLGQPDEADYDEIPRKA
CCHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHH
MEFGAEKARLVDCRSQLVHEGIAAIQSGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKE
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHH
DGVNIWGDGSTYKGNDIERFYRYGLLTNPDLRIYKPWLDQQFIDELGGRAEMSAFMTAAG
CCCEEECCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHCCHHHHHHHHHHHC
FAYKMSSEKAYSTDSNLLGATHEAKDLEHLDSGIRIVVPIMGVPFWREDCAVKAETVSVR
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEEE
FEEGQPVALNGKTFADPVALFLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
ECCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
HIAYERLVTGIHNEDTIEQYRISGMKLGRLLYQGRWFDSQALMLRETAQRWVARAITGEV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEE
TLELRRGNDYSILNTQSPNLTYAPERLSMEKVEDAPFTPGDRIGQLTMRNLDIADTRAKL
EEEEECCCCEEEEECCCCCCEECHHHCCHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHE
DIFAKAGLLSAGEGSHIPKLENDKG
EEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA