Click here to switch to the map view.

The map label for this gene is lpxD

Identifier: 86608764

GI number: 86608764

Start: 1344744

End: 1345835

Strand: Direct

Name: lpxD

Synonym: CYB_1289

Alternate gene names: 86608764

Gene position: 1344744-1345835 (Clockwise)

Preceding gene: 86608763

Following gene: 86608766

Centisome position: 44.14

GC content: 62.18

Gene sequence:

>1092_bases
ATGCAGCTACAGGAAATTGCCCAAAAATTGGGGTGTGCCTACGAGGGGGATCCCACCCTGGAAATTCACAGCGTGGCCAG
CCTGGCGGAGGCTCGCCCAGGGGAGCTGAGCTTTCTCAGCGAGGCCCGCTACCTACCTCTCTTGGAGCAAACCCAGGCCA
GCGCCGTCATCGTGGAGGAAGGGCTGGCTTTGCCCTGTTCCATTGCCTGCTTGCGGGGCAGGGATCCCCGTCTGCTCTTT
GCCCAGGCCATTGAGCTGTTCTACCAACCCTACCGCCTGCCCGTTGGCATCCACCCCACCGCTGTGATCGATCCCAGCGT
CGAGCTGGGGGAGGGGGTGGCCATCGGGCCGCACGCAGTGGTGATGGAGGGAGTGAAAATTGGCGACCACACCCAAATTC
ACCCCAATGTCACCATCTATCCCCACGTGCGGATTGGATCCCGGTGTCAGCTTTTCGCCAACTGCGTTATTCACGAGCGC
ACGGAGATTGGGGATGATTGCCTCATCCACAGCGGCGCTGTCATTGGCGACGACGGCTTTGGCCACATTCCTCTGGCAGA
TGGCTCCTGGCGGCGCATGCTGCAGGCGGGCCGAGTGGTGCTGGAAGACAACGTGGAGGTAGGCAGCAACACCACCATTG
ACCGGGCCGCCGTTGGCGAGACCCGTATTGGCCGCGGCACCAAGATCGACAATCTGGTGCAAATTGGCCACGGCGTGCGC
ACCGGATCCCACTGCCTGATCGTGGCTCAGGTGGGCATTGCCGGCAGCACCCAGTTGGGCCACCACGTCATCTTGGCCGG
GCAATGCGGCCTGGCTGGGCACCTGCATATTGGCGATGGCGTACGCGTGGCTGCCCAGACAGGAGTAACCAGCGACGTCC
CCGCCGGTCAAACGGTGGCCGGCTACCCCCACCAACCCATTGCCGAATGGCGCAAGAGCATGGCCGTCCAGCGGCACTTG
CCTGAGCTGCAACGTACCTTGCGCAAGCTGGAAGCTCGTGTGGCCAAGCTGGAACAGAACAGCACGGATAGAGCCCCCAA
CGCAAAAATGCTCGAAGTGGGCGTCGATCCTGAAACTACATGCTCTAGCTAG

Upstream 100 bases:

>100_bases
ACCGCCATTCTGTTTGGAAACAAACAGAATGCTATCGCTGCATAGTTGAACAGTGATGTTCAGCAGTGTTCAGCGTAAAT
GTATCAGGATGTCAACCCCA

Downstream 100 bases:

>100_bases
AACCTCCTTTGTCAACTTCCCCAGTGGCCTTAGCTACTGACTGGGAGAACTTTTGACCGAAGAGGTTCTATTGAAACGTC
ATGAAGGATATACCTTCTAC

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQLQEIAQKLGCAYEGDPTLEIHSVASLAEARPGELSFLSEARYLPLLEQTQASAVIVEEGLALPCSIACLRGRDPRLLF
AQAIELFYQPYRLPVGIHPTAVIDPSVELGEGVAIGPHAVVMEGVKIGDHTQIHPNVTIYPHVRIGSRCQLFANCVIHER
TEIGDDCLIHSGAVIGDDGFGHIPLADGSWRRMLQAGRVVLEDNVEVGSNTTIDRAAVGETRIGRGTKIDNLVQIGHGVR
TGSHCLIVAQVGIAGSTQLGHHVILAGQCGLAGHLHIGDGVRVAAQTGVTSDVPAGQTVAGYPHQPIAEWRKSMAVQRHL
PELQRTLRKLEARVAKLEQNSTDRAPNAKMLEVGVDPETTCSS

Sequences:

>Translated_363_residues
MQLQEIAQKLGCAYEGDPTLEIHSVASLAEARPGELSFLSEARYLPLLEQTQASAVIVEEGLALPCSIACLRGRDPRLLF
AQAIELFYQPYRLPVGIHPTAVIDPSVELGEGVAIGPHAVVMEGVKIGDHTQIHPNVTIYPHVRIGSRCQLFANCVIHER
TEIGDDCLIHSGAVIGDDGFGHIPLADGSWRRMLQAGRVVLEDNVEVGSNTTIDRAAVGETRIGRGTKIDNLVQIGHGVR
TGSHCLIVAQVGIAGSTQLGHHVILAGQCGLAGHLHIGDGVRVAAQTGVTSDVPAGQTVAGYPHQPIAEWRKSMAVQRHL
PELQRTLRKLEARVAKLEQNSTDRAPNAKMLEVGVDPETTCSS
>Mature_363_residues
MQLQEIAQKLGCAYEGDPTLEIHSVASLAEARPGELSFLSEARYLPLLEQTQASAVIVEEGLALPCSIACLRGRDPRLLF
AQAIELFYQPYRLPVGIHPTAVIDPSVELGEGVAIGPHAVVMEGVKIGDHTQIHPNVTIYPHVRIGSRCQLFANCVIHER
TEIGDDCLIHSGAVIGDDGFGHIPLADGSWRRMLQAGRVVLEDNVEVGSNTTIDRAAVGETRIGRGTKIDNLVQIGHGVR
TGSHCLIVAQVGIAGSTQLGHHVILAGQCGLAGHLHIGDGVRVAAQTGVTSDVPAGQTVAGYPHQPIAEWRKSMAVQRHL
PELQRTLRKLEARVAKLEQNSTDRAPNAKMLEVGVDPETTCSS

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=335, Percent_Identity=34.6268656716418, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786378, Length=230, Percent_Identity=27.8260869565217, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD_SYNJB (Q2JLY8)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477526.1
- ProteinModelPortal:   Q2JLY8
- SMR:   Q2JLY8
- STRING:   Q2JLY8
- GeneID:   3901023
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1289
- TIGR:   CYB_1289
- eggNOG:   COG1044
- HOGENOM:   HBG469615
- OMA:   AKIMKSA
- PhylomeDB:   Q2JLY8
- BioCyc:   SSP321332:CYB_1289-MONOMER
- HAMAP:   MF_00523
- InterPro:   IPR001451
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.-

Molecular weight: Translated: 38863; Mature: 38863

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLQEIAQKLGCAYEGDPTLEIHSVASLAEARPGELSFLSEARYLPLLEQTQASAVIVEE
CCHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHCCCCCCHHHHCCCCEEEEEC
GLALPCSIACLRGRDPRLLFAQAIELFYQPYRLPVGIHPTAVIDPSVELGEGVAIGPHAV
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEECCHHE
VMEGVKIGDHTQIHPNVTIYPHVRIGSRCQLFANCVIHERTEIGDDCLIHSGAVIGDDGF
EEECCEECCCCEECCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCEEECCCCC
GHIPLADGSWRRMLQAGRVVLEDNVEVGSNTTIDRAAVGETRIGRGTKIDNLVQIGHGVR
CEEECCCCHHHHHHHHCCEEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCCC
TGSHCLIVAQVGIAGSTQLGHHVILAGQCGLAGHLHIGDGVRVAAQTGVTSDVPAGQTVA
CCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
GYPHQPIAEWRKSMAVQRHLPELQRTLRKLEARVAKLEQNSTDRAPNAKMLEVGVDPETT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
CSS
CCC
>Mature Secondary Structure
MQLQEIAQKLGCAYEGDPTLEIHSVASLAEARPGELSFLSEARYLPLLEQTQASAVIVEE
CCHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHCCCCCCHHHHCCCCEEEEEC
GLALPCSIACLRGRDPRLLFAQAIELFYQPYRLPVGIHPTAVIDPSVELGEGVAIGPHAV
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEECCHHE
VMEGVKIGDHTQIHPNVTIYPHVRIGSRCQLFANCVIHERTEIGDDCLIHSGAVIGDDGF
EEECCEECCCCEECCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCEEECCCCC
GHIPLADGSWRRMLQAGRVVLEDNVEVGSNTTIDRAAVGETRIGRGTKIDNLVQIGHGVR
CEEECCCCHHHHHHHHCCEEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCCC
TGSHCLIVAQVGIAGSTQLGHHVILAGQCGLAGHLHIGDGVRVAAQTGVTSDVPAGQTVA
CCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
GYPHQPIAEWRKSMAVQRHLPELQRTLRKLEARVAKLEQNSTDRAPNAKMLEVGVDPETT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
CSS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA