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The map label for this gene is dnaB [H]

Identifier: 86608559

GI number: 86608559

Start: 1116586

End: 1118361

Strand: Direct

Name: dnaB [H]

Synonym: CYB_1081

Alternate gene names: 86608559

Gene position: 1116586-1118361 (Clockwise)

Preceding gene: 86608558

Following gene: 86608560

Centisome position: 36.65

GC content: 58.9

Gene sequence:

>1776_bases
GTGATCAGCGATTACGACTTAACGGGTGGGGATCGGCTTCCTCCTCAAAACATTGAAGCCGAGGAGGAAATCCTGGGGGG
CATTCTCCTCGATCCGGATGCGTTGGTGCGGGTCGCGGAGTTTCTGCGTCCAGAGATGTTCTATATCTCTGCCCACCAAG
AGATTTACCGGGCTGCTCTCCAACTGCACAGCCAAGGCCAACCCACTGACCTGATGACTGTGTCTGCCTGGTTGGCGGAT
CATCATCTTCTGGAGCGGGTGGGAGGAACAGGGGCGATCCGTCGTCTGCTGGAGCAAACCGTCAGCAGCGTCAACATCGA
CCAGTATGCTCGCCTGGTGATGGATAAATACATGCGCCGCCAGTTGATCCAGGTGAGCAATACCTTGGCTCGTTTGGCCT
ACGACACCAGTCAGCCCCTGTCTCAGGTGATGGATCAAGCCGAGCAGCAGGTGTTTGGCCTCACCCAAGAGCGAGTGCAG
CGTAGCCTCATCCCGGCTTCTGAGGTTTTGGTCAATATCTTCTCGGACTTGGAAGAGAAGTTCCAAAGCGGGGCACAGCT
TCCGGGGATCCCGACCAAATTCATCGACCTGGATAACCTGACCCAGGGCTTGCAGCGATCCGATTTGCTGATTCTGGCCG
GTCGTCCCTCGATGGGGAAAACCAGTCTTGCCCTCAACATTGCCCAAAATGTGGCTGCCTATGCCAAGCAGCCGGTCGCC
ATCTTTAGCTTGGAAATGTCACGAGAGCAACTGGTGCAGCGGCTTTTGGCCAGCGAAGCCCGCATCGACAGCAGCCGTCT
GCGTTCTGGGCGCATCAGCGAACACGAGTGGCAGCGCCTGGGACAAGCCATTGGTTTTTTATCGCAATTGCCCCTGTTCA
TCGACGATACCCCCGACTGTACCGTTACAGAAATTCGCTCCAAAGCGCGGCGTTTGCAGGCGGAACAGGGGGGAACGTTG
GGGATGGTGTTGATCGACTACCTACAACTGATGCAGGGATCCGCTGCTGACAACCGGGTGCAGGAGCTGTCGCGCATTAC
CCGTGGCCTCAAGGCCATGGCCAAAGAGCTGATGGTGCCGGTGCTGGTGCTTTCTCAGCTCAGTCGAGCTGTTGAGTCTC
GCTCCGATAAGCGCCCGCAACTGGCAGACCTGCGGGAATCGGGCTGTCTGGCCGGGGATACGCAGGTGATGGATGCGGAT
CGCGGCAAGATCTGGCGGCTGGATCAACTGGCGGCCCTGCCGGCTGCGGCGGAACTGCCCCGCCTGCTCAGCTTGAACGG
GCGCGGGCGCTTGGTGCCCCAACAGCCTGTGAAAGTGTTTTGCAGTGGTCGGCAGCCCACCTGTGTTCTGAAGACCCGCC
TCAACTTTTCCATTCGCGCCACCGGCAACCATCCCTTCTTGACCCCAGACGGCTGGAAAACCCTCGACGAGCTGCAATTG
GAGGAAGAGGTGGCGGTGGTAGTGCAGGAGGGGCTGATCTGGGATCCGGTGGTGCTCATCAGCGAGCCGGGGGAGCCTCA
GCCGGTTTACGACATCGAGATGCCCCGCCACCACAACTTTGTGGCCAACGGGCTGCTAGTTCACAACAGCATCGAGCAGG
ATGCCGATCTGGTGATGATGATCTATCGCCCGGAGTACTACGATCCCAACACCAGCGATCGCGGCATTGCCGAAGTCATT
ATCGCCAAACATCGCAACGGCCCCACCGGCACTGTGCGCCTGCTGTTCGAAAGCCAGTACACCCAGTTTCGCAACCTGGC
AGATCCCAACCGCTGA

Upstream 100 bases:

>100_bases
GTATGCTCAGCCTAGAGCTTGACCCGCCTGACGATTGGAGCTGACTGGCTGAGAGGCCGGTTGCCCTAGGGCTGGGTTGA
TTTTCCTATCCTGGCTTGTG

Downstream 100 bases:

>100_bases
GCAACCGATGCCGAAGAGAGATCCGGAGCTGCTGCCTTGGGAACAACCCCGCAAGGTGTTAGCTTAGGATCGCTGCGGGC
CAAAGCGTTGCTCTTGAGCC

Product: replicative DNA helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPDALVRVAEFLRPEMFYISAHQEIYRAALQLHSQGQPTDLMTVSAWLAD
HHLLERVGGTGAIRRLLEQTVSSVNIDQYARLVMDKYMRRQLIQVSNTLARLAYDTSQPLSQVMDQAEQQVFGLTQERVQ
RSLIPASEVLVNIFSDLEEKFQSGAQLPGIPTKFIDLDNLTQGLQRSDLLILAGRPSMGKTSLALNIAQNVAAYAKQPVA
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISEHEWQRLGQAIGFLSQLPLFIDDTPDCTVTEIRSKARRLQAEQGGTL
GMVLIDYLQLMQGSAADNRVQELSRITRGLKAMAKELMVPVLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTQVMDAD
RGKIWRLDQLAALPAAAELPRLLSLNGRGRLVPQQPVKVFCSGRQPTCVLKTRLNFSIRATGNHPFLTPDGWKTLDELQL
EEEVAVVVQEGLIWDPVVLISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADLVMMIYRPEYYDPNTSDRGIAEVI
IAKHRNGPTGTVRLLFESQYTQFRNLADPNR

Sequences:

>Translated_591_residues
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPDALVRVAEFLRPEMFYISAHQEIYRAALQLHSQGQPTDLMTVSAWLAD
HHLLERVGGTGAIRRLLEQTVSSVNIDQYARLVMDKYMRRQLIQVSNTLARLAYDTSQPLSQVMDQAEQQVFGLTQERVQ
RSLIPASEVLVNIFSDLEEKFQSGAQLPGIPTKFIDLDNLTQGLQRSDLLILAGRPSMGKTSLALNIAQNVAAYAKQPVA
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISEHEWQRLGQAIGFLSQLPLFIDDTPDCTVTEIRSKARRLQAEQGGTL
GMVLIDYLQLMQGSAADNRVQELSRITRGLKAMAKELMVPVLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTQVMDAD
RGKIWRLDQLAALPAAAELPRLLSLNGRGRLVPQQPVKVFCSGRQPTCVLKTRLNFSIRATGNHPFLTPDGWKTLDELQL
EEEVAVVVQEGLIWDPVVLISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADLVMMIYRPEYYDPNTSDRGIAEVI
IAKHRNGPTGTVRLLFESQYTQFRNLADPNR
>Mature_591_residues
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPDALVRVAEFLRPEMFYISAHQEIYRAALQLHSQGQPTDLMTVSAWLAD
HHLLERVGGTGAIRRLLEQTVSSVNIDQYARLVMDKYMRRQLIQVSNTLARLAYDTSQPLSQVMDQAEQQVFGLTQERVQ
RSLIPASEVLVNIFSDLEEKFQSGAQLPGIPTKFIDLDNLTQGLQRSDLLILAGRPSMGKTSLALNIAQNVAAYAKQPVA
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISEHEWQRLGQAIGFLSQLPLFIDDTPDCTVTEIRSKARRLQAEQGGTL
GMVLIDYLQLMQGSAADNRVQELSRITRGLKAMAKELMVPVLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTQVMDAD
RGKIWRLDQLAALPAAAELPRLLSLNGRGRLVPQQPVKVFCSGRQPTCVLKTRLNFSIRATGNHPFLTPDGWKTLDELQL
EEEVAVVVQEGLIWDPVVLISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADLVMMIYRPEYYDPNTSDRGIAEVI
IAKHRNGPTGTVRLLFESQYTQFRNLADPNR

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=395, Percent_Identity=40.5063291139241, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 66123; Mature: 66123

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50817 INTEIN_N_TER ; PS50818 INTEIN_C_TER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPDALVRVAEFLRPEMFYISAHQEIYRAAL
CCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
QLHSQGQPTDLMTVSAWLADHHLLERVGGTGAIRRLLEQTVSSVNIDQYARLVMDKYMRR
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QLIQVSNTLARLAYDTSQPLSQVMDQAEQQVFGLTQERVQRSLIPASEVLVNIFSDLEEK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FQSGAQLPGIPTKFIDLDNLTQGLQRSDLLILAGRPSMGKTSLALNIAQNVAAYAKQPVA
HHCCCCCCCCCHHHCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEE
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISEHEWQRLGQAIGFLSQLPLFIDDTPDC
EEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCC
TVTEIRSKARRLQAEQGGTLGMVLIDYLQLMQGSAADNRVQELSRITRGLKAMAKELMVP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTQVMDADRGKIWRLDQLAALPAAAELP
HHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEHHHHHHCCCHHHCC
RLLSLNGRGRLVPQQPVKVFCSGRQPTCVLKTRLNFSIRATGNHPFLTPDGWKTLDELQL
HHHCCCCCCCCCCCCCHHHEECCCCCEEEEEEECCEEEEECCCCCCCCCCCCCHHHHHHH
EEEVAVVVQEGLIWDPVVLISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADLVMM
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHCCEEEECCCCCCCCEEEE
IYRPEYYDPNTSDRGIAEVIIAKHRNGPTGTVRLLFESQYTQFRNLADPNR
EECCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCC
>Mature Secondary Structure
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPDALVRVAEFLRPEMFYISAHQEIYRAAL
CCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
QLHSQGQPTDLMTVSAWLADHHLLERVGGTGAIRRLLEQTVSSVNIDQYARLVMDKYMRR
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QLIQVSNTLARLAYDTSQPLSQVMDQAEQQVFGLTQERVQRSLIPASEVLVNIFSDLEEK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FQSGAQLPGIPTKFIDLDNLTQGLQRSDLLILAGRPSMGKTSLALNIAQNVAAYAKQPVA
HHCCCCCCCCCHHHCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEE
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISEHEWQRLGQAIGFLSQLPLFIDDTPDC
EEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCC
TVTEIRSKARRLQAEQGGTLGMVLIDYLQLMQGSAADNRVQELSRITRGLKAMAKELMVP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTQVMDADRGKIWRLDQLAALPAAAELP
HHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEHHHHHHCCCHHHCC
RLLSLNGRGRLVPQQPVKVFCSGRQPTCVLKTRLNFSIRATGNHPFLTPDGWKTLDELQL
HHHCCCCCCCCCCCCCHHHEECCCCCEEEEEEECCEEEEECCCCCCCCCCCCCHHHHHHH
EEEVAVVVQEGLIWDPVVLISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADLVMM
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHCCEEEECCCCCCCCEEEE
IYRPEYYDPNTSDRGIAEVIIAKHRNGPTGTVRLLFESQYTQFRNLADPNR
EECCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]