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The map label for this gene is rlmN

Identifier: 86608506

GI number: 86608506

Start: 1069918

End: 1070976

Strand: Reverse

Name: rlmN

Synonym: CYB_1027

Alternate gene names: 86608506

Gene position: 1070976-1069918 (Counterclockwise)

Preceding gene: 86608513

Following gene: 86608503

Centisome position: 35.15

GC content: 59.87

Gene sequence:

>1059_bases
ATGTCGGCCCTACCCATTCCGCTGCTGGGGCAATCCTTGTCTGCTCTGAAAGACTGGGCGGTTGCCCAAGGACAGCCGGC
CTATCGCGGGCAACAACTGCATGCTTGGATCTACCAAAAAGGGATCCGCTCCCTGGAACAGGTGACGGTGTTTCCTAGGG
CTTGGCGAGAGGCGGTGCAAAGCTACCCAGTGGGACGCTCGCGCATTGTGCAGCGGACAGAGGCCCGCGATGGTACTGTG
AAGTTTCTGCTGGGGTTGGCGGATGGGCAGTTGATCGAAACGGTAGGGATCCCAACGGCCAAGCGTCTGACGGTCTGCGT
CTCTTCTCAGGTGGGCTGCCCGATGGCCTGCGATTTCTGTGCCACCGGCAAGATGGGCTACCGGCGCAACCTAGAGCTGC
ACGAGATTCTGGATCAGGTGTTGACGGTGCAGGAAGATTTTGGCCGTCGCGTCAGCCATGTGGTCTTTATGGGCATGGGG
GAACCCCTGCTCAACCGCGATACGGTAGTACAAGCCATCCGCAGCCTGAATCAAGATATCGGCATCGGCCAACGGCATAT
CACCCTCTCGACGGTGGGGGTACCCCGGCAAATTGCCTGGCTGGCGCAGCAGGATCTGCAAGTTACCCTGGCCGTCAGCT
TGCATGCCCCCAATCAAGACCTGCGGCAACGGTTGATCCCTTCGGCATCCCACTACCCTCTGGACACTCTGATCCAGGAT
TGCCGCGACTACATGCTGCGCACCGGGCGGCGAGTCAGTTTTGAATATACCCTGCTATCGGGGGTCAACGACCTGCCCAT
TCATGCCCGCCAACTGGCGCAATTGCTGCAGCAGGCCAGCCGGTCAGGGGTACAACTGCACGTGAACCTGATCCCTTACA
ACCCCATCTCTGAAGCTGACTATCAACGCCCCCACCCGACACGGGTCAGAGAGTTCGTCCGCCAGCTCGAACAGCACCAA
GTGCGGGCCACTGTGCGCCAAACCCGTGGCTTGGATGGCAATGCCGCATGTGGTCAACTGCGGGGATCCTTCCTGCGTTC
TTTGCCAGGGGATCTGTAA

Upstream 100 bases:

>100_bases
ACTCGGACGGAGTTCTTGAAGGGGATTGGGTTGCAGGGACAGGCTAGAGTAGAGGAGCCGGACTCCTGTGCCTCTTGAGA
TCTCGTCTAAGATGGTATTG

Downstream 100 bases:

>100_bases
TCGCCGCGCAACGCTTGCGCGAGTACATCAGCTCAGTCCGCAGGCGCGTCGTAACCAGGCGGCATCCTGTTCTGGCAAGG
TGGCGTAGTGCATGACGTAA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSALPIPLLGQSLSALKDWAVAQGQPAYRGQQLHAWIYQKGIRSLEQVTVFPRAWREAVQSYPVGRSRIVQRTEARDGTV
KFLLGLADGQLIETVGIPTAKRLTVCVSSQVGCPMACDFCATGKMGYRRNLELHEILDQVLTVQEDFGRRVSHVVFMGMG
EPLLNRDTVVQAIRSLNQDIGIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLDTLIQD
CRDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNPISEADYQRPHPTRVREFVRQLEQHQ
VRATVRQTRGLDGNAACGQLRGSFLRSLPGDL

Sequences:

>Translated_352_residues
MSALPIPLLGQSLSALKDWAVAQGQPAYRGQQLHAWIYQKGIRSLEQVTVFPRAWREAVQSYPVGRSRIVQRTEARDGTV
KFLLGLADGQLIETVGIPTAKRLTVCVSSQVGCPMACDFCATGKMGYRRNLELHEILDQVLTVQEDFGRRVSHVVFMGMG
EPLLNRDTVVQAIRSLNQDIGIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLDTLIQD
CRDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNPISEADYQRPHPTRVREFVRQLEQHQ
VRATVRQTRGLDGNAACGQLRGSFLRSLPGDL
>Mature_351_residues
SALPIPLLGQSLSALKDWAVAQGQPAYRGQQLHAWIYQKGIRSLEQVTVFPRAWREAVQSYPVGRSRIVQRTEARDGTVK
FLLGLADGQLIETVGIPTAKRLTVCVSSQVGCPMACDFCATGKMGYRRNLELHEILDQVLTVQEDFGRRVSHVVFMGMGE
PLLNRDTVVQAIRSLNQDIGIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLDTLIQDC
RDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNPISEADYQRPHPTRVREFVRQLEQHQV
RATVRQTRGLDGNAACGQLRGSFLRSLPGDL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=353, Percent_Identity=35.9773371104816, Blast_Score=221, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SYNJB (Q2JMN2)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477268.1
- ProteinModelPortal:   Q2JMN2
- STRING:   Q2JMN2
- GeneID:   3902170
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1027
- TIGR:   CYB_1027
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q2JMN2
- ProtClustDB:   PRK14457
- BioCyc:   SSP321332:CYB_1027-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39405; Mature: 39274

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPIPLLGQSLSALKDWAVAQGQPAYRGQQLHAWIYQKGIRSLEQVTVFPRAWREAVQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
SYPVGRSRIVQRTEARDGTVKFLLGLADGQLIETVGIPTAKRLTVCVSSQVGCPMACDFC
HCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEHHCCCCCHHHEEEEECCCCCCCCEECHH
ATGKMGYRRNLELHEILDQVLTVQEDFGRRVSHVVFMGMGEPLLNRDTVVQAIRSLNQDI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHC
GIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLDTLIQD
CCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHH
CRDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNPISEAD
HHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
YQRPHPTRVREFVRQLEQHQVRATVRQTRGLDGNAACGQLRGSFLRSLPGDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SALPIPLLGQSLSALKDWAVAQGQPAYRGQQLHAWIYQKGIRSLEQVTVFPRAWREAVQ
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
SYPVGRSRIVQRTEARDGTVKFLLGLADGQLIETVGIPTAKRLTVCVSSQVGCPMACDFC
HCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEHHCCCCCHHHEEEEECCCCCCCCEECHH
ATGKMGYRRNLELHEILDQVLTVQEDFGRRVSHVVFMGMGEPLLNRDTVVQAIRSLNQDI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHC
GIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLDTLIQD
CCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHH
CRDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNPISEAD
HHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
YQRPHPTRVREFVRQLEQHQVRATVRQTRGLDGNAACGQLRGSFLRSLPGDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA