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The map label for this gene is yrkF [H]

Identifier: 86608504

GI number: 86608504

Start: 1067556

End: 1067930

Strand: Direct

Name: yrkF [H]

Synonym: CYB_1025

Alternate gene names: 86608504

Gene position: 1067556-1067930 (Clockwise)

Preceding gene: 86608500

Following gene: 86608505

Centisome position: 35.04

GC content: 58.4

Gene sequence:

>375_bases
ATGGCTCCCCAGCACTCTCCAGCTTTCTTGGCCCTGGTCAACGAGGCCAAAAGCCGCATCCAAGAACTGACAGTCGAGCA
GGTCAGGCAAAAACAACTGCAAGGGGATCCCTTCTATTTTGTGGATGTGCGGGAAGAGTCGGAGTGGCAAGCCGGCCACG
CTGCCGGAGCCATCCACTTGAGCAAAGGGATCATTGAGCGGGACATCGAGAGGGTGATCCCCGACAAAGAAGCGGAGATC
GTCCTGTACTGCGGCGGCGGGTTTCGTTCGGCGTTGGCAGCCGATAATCTACAGAAAATGGGCTATCGTCGGGTGATCTC
GATGGATGGGGGCATGCGGGCTTGGCGGGAAGCGGGTTTTCCTGAGGAGCGCTAG

Upstream 100 bases:

>100_bases
AAATCTGGATCCCAGTCTAGCCTTAGGGCAGTTGGGATCCCAAAGTCCAGACCAGATAAGATCGATTGGAATAAGCTTTG
GCTCATAGCGCCTGTTCTCC

Downstream 100 bases:

>100_bases
AGCTGGCGGATTTCTTCCCTCACCCATAGGAAGAGGGAGGTATTGGCTGCTATCTGGCTGCTATCTGAAATGGCCGACCA
CTGAAGGCGGGAAGTGGGGG

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MAPQHSPAFLALVNEAKSRIQELTVEQVRQKQLQGDPFYFVDVREESEWQAGHAAGAIHLSKGIIERDIERVIPDKEAEI
VLYCGGGFRSALAADNLQKMGYRRVISMDGGMRAWREAGFPEER

Sequences:

>Translated_124_residues
MAPQHSPAFLALVNEAKSRIQELTVEQVRQKQLQGDPFYFVDVREESEWQAGHAAGAIHLSKGIIERDIERVIPDKEAEI
VLYCGGGFRSALAADNLQKMGYRRVISMDGGMRAWREAGFPEER
>Mature_123_residues
APQHSPAFLALVNEAKSRIQELTVEQVRQKQLQGDPFYFVDVREESEWQAGHAAGAIHLSKGIIERDIERVIPDKEAEIV
LYCGGGFRSALAADNLQKMGYRRVISMDGGMRAWREAGFPEER

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001455 [H]

Pfam domain/function: PF00581 Rhodanese; PF01206 SirA [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 13972; Mature: 13841

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPQHSPAFLALVNEAKSRIQELTVEQVRQKQLQGDPFYFVDVREESEWQAGHAAGAIHL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
SKGIIERDIERVIPDKEAEIVLYCGGGFRSALAADNLQKMGYRRVISMDGGMRAWREAGF
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCC
PEER
CCCC
>Mature Secondary Structure 
APQHSPAFLALVNEAKSRIQELTVEQVRQKQLQGDPFYFVDVREESEWQAGHAAGAIHL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
SKGIIERDIERVIPDKEAEIVLYCGGGFRSALAADNLQKMGYRRVISMDGGMRAWREAGF
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCC
PEER
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]