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The map label for this gene is mrdA [H]

Identifier: 86608375

GI number: 86608375

Start: 927346

End: 929199

Strand: Direct

Name: mrdA [H]

Synonym: CYB_0895

Alternate gene names: 86608375

Gene position: 927346-929199 (Clockwise)

Preceding gene: 86608373

Following gene: 86608376

Centisome position: 30.44

GC content: 64.24

Gene sequence:

>1854_bases
TTGGCAGGACGGGATCCGCAAGCCCTGTTCTCCCCCCATTCCAGACCGCCTGCCCCCCGCCGGGTACTTCCCCCACCCCT
GCCGGACGGCGGTAGCCCTCGCTGGCTGGGATCCGCATCGGCCAAAACGGTGGGGCAGGGCTATCGGGCCGTTGTCTTGC
TCTCGCTGACCAGTCTGCTGCTGTTTGGAGGGCTGGGATCCCGTCTGGCCTACCTGCAGCTAGCGCGCACCCAGTATTAC
CGCCAGCTGGCCGATACCAACCGCATTCGCCTGTTGCCCCAGCCGCCAGAACGCGGACGCATTGTGGATCGCAACGGCGT
ATTGCTGGCGAGCAGCCGATTGTCCCACTCGGTCTTCCTCTGGCCTCTGGTGCAGTCGCGGCAGCGCTGGCAGGAGATGA
TCCCCAAGTTGGCAGAATACTTGAACATGGCCCCCGAGGAGATCGCCCAGAAACTGGAACAGGCCGGATATCGTTCCCCG
TTTCCCGTGCGCATCCTGCGCAACGCCAGCCCCCAGGTGGTCATTCAACTGGAGGAGATGAGCCGCGAGCTGCCGGGGGT
GATGGTGGAGGCGGAGACGGCGCGGTTTTATCCCCATGGCGACTTGGCTGCTCACCTGCTGGGCTACACCGGCGAGATCC
CCCTAGAGCAACTGCGGGCCGACCCTAGCTATCGCCTGGGGGATATCGTTGGCCTGATGGGAGTGGAGGAATTGTTCGAG
AACCATCTGCGCGGCCAATGGGGCGGGCGACAGGTGGAGGTGGACGCCATGGGAGAGGTGCTGCGGGTGCTGGGGGAACA
AGCGCCCCAACCGGGCAACACCCTGCAGCTCACTTTGGATGTGCGGCTGCAGCAGGCGGCAGAGCGGGCCCTCGGCCAGC
AAAAAGGAGCGGTGATCGCCATGGATCCCAGCGATGGCTCGATCCTGGCCATGGCCAGCTATCCCACCTTTGATCCCAAC
CTCTTTTCCAGCCGCATCACCCAAGCCCAGTGGGAGAGCCTGCAGCAGCAGGATTTTCCCTTCCTCAACCGCGGCCTGCG
GCCCTATCCCCCCGCCAGCACCTACAAAATCATCACCACCGCTGCCGGGATTGAGTCGGGAGCCTTTCCCCCGAATACGG
TGCTGCAGACCGGCCCCTACATCCAGGTGGGAGGATGGCGCTTTTGGGACTGGAACCGGGCCGGCTTCGGGCGGCTGAAC
TACCGGCAGGCGATGGCCTACAGCAGCGACACCTTTTTCTACCAAATCGCCCTCGGCACCAAGGCTGCCCCTTTGCAAGA
ATGGTCGCGCCGCTTTGGGTTGGGACAACTGACCGGGATTGGCCTCAAAGGGGAAGCCGCGGGTCTGGTGCCGGATGCTG
AGTGGAAGCAGCGCCACTGGCGCGAGCCCTGGTATGTGGGCGACACGGTCAACATGTCCATCGGCCAGGGATTTATTACC
GCTACGCCGCTGCAAATGGCGGTGGTAACGGCGGCAGTGGCCAATGGGGGCTGGCGGGTGCGCCCCTTGCTCTCGCACCA
GGTGATCCCGGAAAGCCCTGTGCCGCTGCGCCAAAAGGTAGGTCTTTCCCCTGCCACCCTGGAGATCCTGCAGCAGGGGC
TGCGAGATGTCGTGGTTTACGGCACTGGCGGCAACGCCAACCTGGGGCCAGGATTTCCCCCAACGGCTGGTAAAACCGGC
ACCGCAGAGGATCCGCCGCGGCGATCCCACGCTTGGTATGTTGGCTATGCTCCCTACGACCGGCCCGAATTGGTGGTCGT
GGTCTTTCTGGAAAACAGCGGCGGTGGGGGTGGATCACGGGCCGCTCCCATCTTTCGAGAGGTGATGCGGGCCTACTTCC
GACCCGAGTCGTGA

Upstream 100 bases:

>100_bases
GGCATCTCCTCTCGATTTTGGGTGTATCGGTTGCAATTCTTCTGGCAATTTCATGGCCAAACTCTACCCCGTCAGCAACG
CCGCGCGAAGCCCCCACACC

Downstream 100 bases:

>100_bases
GCCCTAGCTTTCTGTCGGATCCGAGCCAACTGGGTATATGCAGTCTCTTGTCTCCCAGAAAATTGCGTTAAGATCCCCTA
AAACCCCTTGCCCAGCAGGC

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MAGRDPQALFSPHSRPPAPRRVLPPPLPDGGSPRWLGSASAKTVGQGYRAVVLLSLTSLLLFGGLGSRLAYLQLARTQYY
RQLADTNRIRLLPQPPERGRIVDRNGVLLASSRLSHSVFLWPLVQSRQRWQEMIPKLAEYLNMAPEEIAQKLEQAGYRSP
FPVRILRNASPQVVIQLEEMSRELPGVMVEAETARFYPHGDLAAHLLGYTGEIPLEQLRADPSYRLGDIVGLMGVEELFE
NHLRGQWGGRQVEVDAMGEVLRVLGEQAPQPGNTLQLTLDVRLQQAAERALGQQKGAVIAMDPSDGSILAMASYPTFDPN
LFSSRITQAQWESLQQQDFPFLNRGLRPYPPASTYKIITTAAGIESGAFPPNTVLQTGPYIQVGGWRFWDWNRAGFGRLN
YRQAMAYSSDTFFYQIALGTKAAPLQEWSRRFGLGQLTGIGLKGEAAGLVPDAEWKQRHWREPWYVGDTVNMSIGQGFIT
ATPLQMAVVTAAVANGGWRVRPLLSHQVIPESPVPLRQKVGLSPATLEILQQGLRDVVVYGTGGNANLGPGFPPTAGKTG
TAEDPPRRSHAWYVGYAPYDRPELVVVVFLENSGGGGGSRAAPIFREVMRAYFRPES

Sequences:

>Translated_617_residues
MAGRDPQALFSPHSRPPAPRRVLPPPLPDGGSPRWLGSASAKTVGQGYRAVVLLSLTSLLLFGGLGSRLAYLQLARTQYY
RQLADTNRIRLLPQPPERGRIVDRNGVLLASSRLSHSVFLWPLVQSRQRWQEMIPKLAEYLNMAPEEIAQKLEQAGYRSP
FPVRILRNASPQVVIQLEEMSRELPGVMVEAETARFYPHGDLAAHLLGYTGEIPLEQLRADPSYRLGDIVGLMGVEELFE
NHLRGQWGGRQVEVDAMGEVLRVLGEQAPQPGNTLQLTLDVRLQQAAERALGQQKGAVIAMDPSDGSILAMASYPTFDPN
LFSSRITQAQWESLQQQDFPFLNRGLRPYPPASTYKIITTAAGIESGAFPPNTVLQTGPYIQVGGWRFWDWNRAGFGRLN
YRQAMAYSSDTFFYQIALGTKAAPLQEWSRRFGLGQLTGIGLKGEAAGLVPDAEWKQRHWREPWYVGDTVNMSIGQGFIT
ATPLQMAVVTAAVANGGWRVRPLLSHQVIPESPVPLRQKVGLSPATLEILQQGLRDVVVYGTGGNANLGPGFPPTAGKTG
TAEDPPRRSHAWYVGYAPYDRPELVVVVFLENSGGGGGSRAAPIFREVMRAYFRPES
>Mature_616_residues
AGRDPQALFSPHSRPPAPRRVLPPPLPDGGSPRWLGSASAKTVGQGYRAVVLLSLTSLLLFGGLGSRLAYLQLARTQYYR
QLADTNRIRLLPQPPERGRIVDRNGVLLASSRLSHSVFLWPLVQSRQRWQEMIPKLAEYLNMAPEEIAQKLEQAGYRSPF
PVRILRNASPQVVIQLEEMSRELPGVMVEAETARFYPHGDLAAHLLGYTGEIPLEQLRADPSYRLGDIVGLMGVEELFEN
HLRGQWGGRQVEVDAMGEVLRVLGEQAPQPGNTLQLTLDVRLQQAAERALGQQKGAVIAMDPSDGSILAMASYPTFDPNL
FSSRITQAQWESLQQQDFPFLNRGLRPYPPASTYKIITTAAGIESGAFPPNTVLQTGPYIQVGGWRFWDWNRAGFGRLNY
RQAMAYSSDTFFYQIALGTKAAPLQEWSRRFGLGQLTGIGLKGEAAGLVPDAEWKQRHWREPWYVGDTVNMSIGQGFITA
TPLQMAVVTAAVANGGWRVRPLLSHQVIPESPVPLRQKVGLSPATLEILQQGLRDVVVYGTGGNANLGPGFPPTAGKTGT
AEDPPRRSHAWYVGYAPYDRPELVVVVFLENSGGGGGSRAAPIFREVMRAYFRPES

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=594, Percent_Identity=33.3333333333333, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1786272, Length=586, Percent_Identity=28.3276450511945, Blast_Score=168, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 68004; Mature: 67872

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRDPQALFSPHSRPPAPRRVLPPPLPDGGSPRWLGSASAKTVGQGYRAVVLLSLTSLL
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
LFGGLGSRLAYLQLARTQYYRQLADTNRIRLLPQPPERGRIVDRNGVLLASSRLSHSVFL
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCEEEEECCCCCEEEE
WPLVQSRQRWQEMIPKLAEYLNMAPEEIAQKLEQAGYRSPFPVRILRNASPQVVIQLEEM
EHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEHHHH
SRELPGVMVEAETARFYPHGDLAAHLLGYTGEIPLEQLRADPSYRLGDIVGLMGVEELFE
HHHCCCEEEEECCEEECCCCHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
NHLRGQWGGRQVEVDAMGEVLRVLGEQAPQPGNTLQLTLDVRLQQAAERALGQQKGAVIA
HHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCEEEE
MDPSDGSILAMASYPTFDPNLFSSRITQAQWESLQQQDFPFLNRGLRPYPPASTYKIITT
ECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEE
AAGIESGAFPPNTVLQTGPYIQVGGWRFWDWNRAGFGRLNYRQAMAYSSDTFFYQIALGT
ECCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEEECC
KAAPLQEWSRRFGLGQLTGIGLKGEAAGLVPDAEWKQRHWREPWYVGDTVNMSIGQGFIT
CCCHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCEECCEEEECCCCCEEE
ATPLQMAVVTAAVANGGWRVRPLLSHQVIPESPVPLRQKVGLSPATLEILQQGLRDVVVY
ECHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCEEEEE
GTGGNANLGPGFPPTAGKTGTAEDPPRRSHAWYVGYAPYDRPELVVVVFLENSGGGGGSR
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCC
AAPIFREVMRAYFRPES
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AGRDPQALFSPHSRPPAPRRVLPPPLPDGGSPRWLGSASAKTVGQGYRAVVLLSLTSLL
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
LFGGLGSRLAYLQLARTQYYRQLADTNRIRLLPQPPERGRIVDRNGVLLASSRLSHSVFL
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCEEEEECCCCCEEEE
WPLVQSRQRWQEMIPKLAEYLNMAPEEIAQKLEQAGYRSPFPVRILRNASPQVVIQLEEM
EHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEHHHH
SRELPGVMVEAETARFYPHGDLAAHLLGYTGEIPLEQLRADPSYRLGDIVGLMGVEELFE
HHHCCCEEEEECCEEECCCCHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
NHLRGQWGGRQVEVDAMGEVLRVLGEQAPQPGNTLQLTLDVRLQQAAERALGQQKGAVIA
HHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCEEEE
MDPSDGSILAMASYPTFDPNLFSSRITQAQWESLQQQDFPFLNRGLRPYPPASTYKIITT
ECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEE
AAGIESGAFPPNTVLQTGPYIQVGGWRFWDWNRAGFGRLNYRQAMAYSSDTFFYQIALGT
ECCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEEECC
KAAPLQEWSRRFGLGQLTGIGLKGEAAGLVPDAEWKQRHWREPWYVGDTVNMSIGQGFIT
CCCHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCEECCEEEECCCCCEEE
ATPLQMAVVTAAVANGGWRVRPLLSHQVIPESPVPLRQKVGLSPATLEILQQGLRDVVVY
ECHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCEEEEE
GTGGNANLGPGFPPTAGKTGTAEDPPRRSHAWYVGYAPYDRPELVVVVFLENSGGGGGSR
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCC
AAPIFREVMRAYFRPES
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]