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The map label for this gene is kefA [H]

Identifier: 86608290

GI number: 86608290

Start: 835786

End: 837645

Strand: Direct

Name: kefA [H]

Synonym: CYB_0808

Alternate gene names: 86608290

Gene position: 835786-837645 (Clockwise)

Preceding gene: 86608289

Following gene: 86608291

Centisome position: 27.43

GC content: 59.46

Gene sequence:

>1860_bases
ATGGGCGTTCGTATCAAACCGGCCATGCAGGACTCTGTCCCATTTCGGCGAGCAGTTCTCTTGATTGGGCTGGCGGTGGG
GATAGGTCTCTGGCTTACCCCTGCCGTTGCCTCTGCCCCTTCATTGGCTCAAGCCCTTGAATTTCGGGATCCCAACTTTT
TCCTGGCCGATCCCCGACCCACACCGGCTGAAGCGCCAACTCCTGAGGGATCCCCTGCTCCGGCTCCCAGCCCAGTCGGG
ATCCCTTTGACCGGCCAAGAGGGCACAGAGGTGTTGGTCGGGAACATACCGGTGTTCCGCCTGCAGACGGCAGACTACCC
CAGCGATGTGCGGGCCGACAACGTTCGGGCGATCATCGAGCGCTTTCTCGAAGAAGGGCAGTTGCCACAGCCAGAGGTAC
GGGTAGCCCAGGATGCCAATGGGCAATACATCTTACGCCTGGGGGAAAGAGGCCGCTTTGAGGCCACCCAACGGTATTTG
TTTACCGTCACCTATGCCGATGCTGCCGCGGAGCGCAGGGTGGCACCGGGTCAGGTGAACCTGAATGACGTTCGTCTGGT
GGCTAACAGTTGGGCGCGGCGGTTGGAGCAAGCGATTGCCGATTACCGCGAAGATCTGCTGGCTCTGCAGCGGGCCCAGG
ATCCGCGCGTGCTGGTTCTGACCCTTTTGGCAGGGATCGCCACCCTGGTGGTGGGGTTCTTTCTCTGGCGGCTGTTCGAC
CGCAGCCTAACCCGCTTGCAACGCTGGTTGGAGCTTAAGGCCGGAGAGGCCTGGGGCACCTGGATTGATGTGGGTGGGAT
GCTGCTGCGGGGCGTGGGGGCAATCCTCATCGCCGGCATCTCGCTGGATCGCGGCATCAGCTTCATACCTGCCCTGCGAC
CTTTTCAGCGCTCCTTTTACCTGGGCCTGGGTCGTGTGGTCGCCACGGCTCTGGGCGTTCTCACCCAGCCTCTGCCCAAC
TCCAGCGTTTCGATTGCCAGCTTGCTGGTCTTCCTCGTCCTCTCCATCTTGGTTTTCACCGGCGCCCACTACGTCAGTGT
TGGCTTCAAGCAACGCTTTCTGGCACGCCTAGGGCTGGATCTGGGCACCCAAGAGTCTTCGGCGGCCATCTTCAAGTACA
TCCTGACCCTGTTCGGCATCCTTCTGGTCTTGCCCTTCAGCGGCCTTAATCTGGGCTCCTTGGCGGTTATTGCCGGGGCG
GTGGGTTTGGGGATCGGCTTTGGTTTGCAAAACCTCTCCAACAACTACATCAGCTACATTGCCATTTTGCTGGAGCGCCC
CATCCAAATCGGGGATCTGGTGGAAGTGGACAACCTGCTCGGCACAGTGGAGCGCATTGGCCCACGGGCTACCGTCTTGC
GCACCCTGGATCGCATCTTTGTGATCGTGCCCAACTCCCGCTTTACCGAATCAAAGGTGGTGAACTGGAGCTACCGGGAT
CCCCGCTGTCGCATCCACATTCCGGTCGGCGTGGCCTACGGCTCCGATACCGCCTTGGTTACCGAAGCTCTGCTCAGCGC
CGCCAGAGAAAATGCTCGGGTGTTGAGCAGCCCTGCCCCCCAAGTCTGGCTCAAGTCCTTTGGCCCTTCTGCACTTAACT
TTGAGCTGCTGGTGTGGATCAACCGTCCTCAAGATCAGTTTGTCTTGATCAGCGAGCTAAACTTCCTAATCGAGGCTGAG
TTTCGGCGACGTGGGATCCGCATCCCCTTCCCGCAACAAGATATTCATATCCGCAGCCTAGAGGGATTTGCCCCCCTCAG
TCAAAATGGCGGATCCCCTTCTTTGCCCGATACCGACTCCATTCACTCTGCCCTCAAAGAAGCGGCTCAATCGCCGGAAG
ACCAAAATGGTGTTTCCTAA

Upstream 100 bases:

>100_bases
CCTCAGGGCATCTAAAATCCTTGCTCAGATCGGCCAAAATTGAGGTCGGCTTGTGCCACCATGAGCTTACAGGGCAGCCC
TCAGGGATCCCCAATTATCG

Downstream 100 bases:

>100_bases
GCTATTGCCTGCCATTGACACCCTATCGACACCATGTTGCAGCGGTTAGATGAGGTACTGGGAAGCTTTGGCAGCCATCC
CCGTTGGGCTGAACTGAAGC

Product: small conductance mechanosensitive ion channel (MscS) family protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MGVRIKPAMQDSVPFRRAVLLIGLAVGIGLWLTPAVASAPSLAQALEFRDPNFFLADPRPTPAEAPTPEGSPAPAPSPVG
IPLTGQEGTEVLVGNIPVFRLQTADYPSDVRADNVRAIIERFLEEGQLPQPEVRVAQDANGQYILRLGERGRFEATQRYL
FTVTYADAAAERRVAPGQVNLNDVRLVANSWARRLEQAIADYREDLLALQRAQDPRVLVLTLLAGIATLVVGFFLWRLFD
RSLTRLQRWLELKAGEAWGTWIDVGGMLLRGVGAILIAGISLDRGISFIPALRPFQRSFYLGLGRVVATALGVLTQPLPN
SSVSIASLLVFLVLSILVFTGAHYVSVGFKQRFLARLGLDLGTQESSAAIFKYILTLFGILLVLPFSGLNLGSLAVIAGA
VGLGIGFGLQNLSNNYISYIAILLERPIQIGDLVEVDNLLGTVERIGPRATVLRTLDRIFVIVPNSRFTESKVVNWSYRD
PRCRIHIPVGVAYGSDTALVTEALLSAARENARVLSSPAPQVWLKSFGPSALNFELLVWINRPQDQFVLISELNFLIEAE
FRRRGIRIPFPQQDIHIRSLEGFAPLSQNGGSPSLPDTDSIHSALKEAAQSPEDQNGVS

Sequences:

>Translated_619_residues
MGVRIKPAMQDSVPFRRAVLLIGLAVGIGLWLTPAVASAPSLAQALEFRDPNFFLADPRPTPAEAPTPEGSPAPAPSPVG
IPLTGQEGTEVLVGNIPVFRLQTADYPSDVRADNVRAIIERFLEEGQLPQPEVRVAQDANGQYILRLGERGRFEATQRYL
FTVTYADAAAERRVAPGQVNLNDVRLVANSWARRLEQAIADYREDLLALQRAQDPRVLVLTLLAGIATLVVGFFLWRLFD
RSLTRLQRWLELKAGEAWGTWIDVGGMLLRGVGAILIAGISLDRGISFIPALRPFQRSFYLGLGRVVATALGVLTQPLPN
SSVSIASLLVFLVLSILVFTGAHYVSVGFKQRFLARLGLDLGTQESSAAIFKYILTLFGILLVLPFSGLNLGSLAVIAGA
VGLGIGFGLQNLSNNYISYIAILLERPIQIGDLVEVDNLLGTVERIGPRATVLRTLDRIFVIVPNSRFTESKVVNWSYRD
PRCRIHIPVGVAYGSDTALVTEALLSAARENARVLSSPAPQVWLKSFGPSALNFELLVWINRPQDQFVLISELNFLIEAE
FRRRGIRIPFPQQDIHIRSLEGFAPLSQNGGSPSLPDTDSIHSALKEAAQSPEDQNGVS
>Mature_618_residues
GVRIKPAMQDSVPFRRAVLLIGLAVGIGLWLTPAVASAPSLAQALEFRDPNFFLADPRPTPAEAPTPEGSPAPAPSPVGI
PLTGQEGTEVLVGNIPVFRLQTADYPSDVRADNVRAIIERFLEEGQLPQPEVRVAQDANGQYILRLGERGRFEATQRYLF
TVTYADAAAERRVAPGQVNLNDVRLVANSWARRLEQAIADYREDLLALQRAQDPRVLVLTLLAGIATLVVGFFLWRLFDR
SLTRLQRWLELKAGEAWGTWIDVGGMLLRGVGAILIAGISLDRGISFIPALRPFQRSFYLGLGRVVATALGVLTQPLPNS
SVSIASLLVFLVLSILVFTGAHYVSVGFKQRFLARLGLDLGTQESSAAIFKYILTLFGILLVLPFSGLNLGSLAVIAGAV
GLGIGFGLQNLSNNYISYIAILLERPIQIGDLVEVDNLLGTVERIGPRATVLRTLDRIFVIVPNSRFTESKVVNWSYRDP
RCRIHIPVGVAYGSDTALVTEALLSAARENARVLSSPAPQVWLKSFGPSALNFELLVWINRPQDQFVLISELNFLIEAEF
RRRGIRIPFPQQDIHIRSLEGFAPLSQNGGSPSLPDTDSIHSALKEAAQSPEDQNGVS

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=262, Percent_Identity=34.7328244274809, Blast_Score=166, Evalue=6e-42,
Organism=Escherichia coli, GI2367355, Length=283, Percent_Identity=34.6289752650177, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=26.8939393939394, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787591, Length=238, Percent_Identity=24.3697478991597, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 67569; Mature: 67438

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVRIKPAMQDSVPFRRAVLLIGLAVGIGLWLTPAVASAPSLAQALEFRDPNFFLADPRP
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCC
TPAEAPTPEGSPAPAPSPVGIPLTGQEGTEVLVGNIPVFRLQTADYPSDVRADNVRAIIE
CCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHH
RFLEEGQLPQPEVRVAQDANGQYILRLGERGRFEATQRYLFTVTYADAAAERRVAPGQVN
HHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCHHCCEEEEEEEECCHHHHCCCCCCCCC
LNDVRLVANSWARRLEQAIADYREDLLALQRAQDPRVLVLTLLAGIATLVVGFFLWRLFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RSLTRLQRWLELKAGEAWGTWIDVGGMLLRGVGAILIAGISLDRGISFIPALRPFQRSFY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHH
LGLGRVVATALGVLTQPLPNSSVSIASLLVFLVLSILVFTGAHYVSVGFKQRFLARLGLD
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHCCC
LGTQESSAAIFKYILTLFGILLVLPFSGLNLGSLAVIAGAVGLGIGFGLQNLSNNYISYI
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AILLERPIQIGDLVEVDNLLGTVERIGPRATVLRTLDRIFVIVPNSRFTESKVVNWSYRD
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCCEEEECCCC
PRCRIHIPVGVAYGSDTALVTEALLSAARENARVLSSPAPQVWLKSFGPSALNFELLVWI
CCEEEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHCCCCCCCEEEEEEE
NRPQDQFVLISELNFLIEAEFRRRGIRIPFPQQDIHIRSLEGFAPLSQNGGSPSLPDTDS
ECCCCCEEEHHHHHHHEEEHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHH
IHSALKEAAQSPEDQNGVS
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GVRIKPAMQDSVPFRRAVLLIGLAVGIGLWLTPAVASAPSLAQALEFRDPNFFLADPRP
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCC
TPAEAPTPEGSPAPAPSPVGIPLTGQEGTEVLVGNIPVFRLQTADYPSDVRADNVRAIIE
CCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHH
RFLEEGQLPQPEVRVAQDANGQYILRLGERGRFEATQRYLFTVTYADAAAERRVAPGQVN
HHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCHHCCEEEEEEEECCHHHHCCCCCCCCC
LNDVRLVANSWARRLEQAIADYREDLLALQRAQDPRVLVLTLLAGIATLVVGFFLWRLFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RSLTRLQRWLELKAGEAWGTWIDVGGMLLRGVGAILIAGISLDRGISFIPALRPFQRSFY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHH
LGLGRVVATALGVLTQPLPNSSVSIASLLVFLVLSILVFTGAHYVSVGFKQRFLARLGLD
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHCCC
LGTQESSAAIFKYILTLFGILLVLPFSGLNLGSLAVIAGAVGLGIGFGLQNLSNNYISYI
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AILLERPIQIGDLVEVDNLLGTVERIGPRATVLRTLDRIFVIVPNSRFTESKVVNWSYRD
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCCEEEECCCC
PRCRIHIPVGVAYGSDTALVTEALLSAARENARVLSSPAPQVWLKSFGPSALNFELLVWI
CCEEEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHCCCCCCCEEEEEEE
NRPQDQFVLISELNFLIEAEFRRRGIRIPFPQQDIHIRSLEGFAPLSQNGGSPSLPDTDS
ECCCCCEEEHHHHHHHEEEHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHH
IHSALKEAAQSPEDQNGVS
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]