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The map label for this gene is pheS [H]

Identifier: 86608219

GI number: 86608219

Start: 759278

End: 760279

Strand: Direct

Name: pheS [H]

Synonym: CYB_0735

Alternate gene names: 86608219

Gene position: 759278-760279 (Clockwise)

Preceding gene: 86608218

Following gene: 86608233

Centisome position: 24.92

GC content: 58.88

Gene sequence:

>1002_bases
ATGCAAGACGAAAAAGACACGCTTCGCCAACAGCTTCTCACCCTGCAAAGCGAGGCCCTCGCCCAAGTGGAACAAGCTGA
CGGTCTGGAGGCTCTGGAACAGATCCGCATCGACTACCTGGGCAAGAAAGGTAAGCTCTCCAAAATCTTGGGGGGAATGG
GGAAGCTGCCCCCAGAAGAACGGCCTGCCATTGGCGCTTTGGCCAACACCTTTAAAGAGGCCCTCCAATCCAGTCTGGAG
CAGCGCAAACAGGCCCTTGGTGAAGCTCAACTGCTGGCCCGCCTAGAAGCAGAGCGCCTGGATGTCACTATGCCCGGGCC
CCGTCGGCCTCTGGGGCGGATCCATCCGCTGCAGGCCACCATCGACCGCATGCTCGATATCTTTGTGGGAATGGGCTACA
CCGTGGTGCATGGGCCTGAGCTCGAGACGGAGTACTACAACTTCGAGGCCCTCAACACCCCTGCCGACCATCCGGCGCGG
GATATGCAGGATACCCTCTACCTGGCCAATGGCTACCTGATGCGTACCCAAACCTCCTCGGTGCAGGTGCGCTACATGGA
GCAACACCAGCCGCCGCTGCGCATTGTGCATGCCGGTCGGGTGATGCGCCGCGATACCGTGGATGCCACCCACTCCGCTG
TGTTTCACCAGATGGAAATTTTGGCGGTGGGCGAGAACCTCACCTTTTCCGACCTGAAGGGCACCCTCAACACGTTCATC
ACCCGCCTATTGGGCAAGCGAGAAACCCGCTTTCGGCCCAGCTATTTTCCCTTTACCGAGCCTTCTGCTGAGATGGACGT
GCGCTTTGGCTCGAAATGGCTGGAAATCTTCGGCTGCGGCATGGTGGATCCCAATGTATTTCAAGCGGTGGGCTACGACC
CGGAGCGCTACACCGGATTTGCGGCAGGGATCGGCATTGAGCGGATGGCCATGCTCCTCCACGGCATTCAGGATATCCGC
CTGTTTTATACCAGCGACCTGCGCTTTCTGTCTCAGTTTTAG

Upstream 100 bases:

>100_bases
CCCCATCGGATGCAAAGGCGCGACCGAGCTCCCTCCTGTGATACCTTTGTAGGGATTCCTTACAACCCCAGGGATCCCTT
CTGCCCTTGGAATCGGCCCC

Downstream 100 bases:

>100_bases
GTGGCTATCTTATCTGAGGAACAAAACCCTCGTCCTGAGCCCTGCAAGACCTTCAGAAAACCCTCTTTCAGGAAAAGGTT
TGCTTGTAGATCAACTGCAA

Product: phenylalanyl-tRNA synthetase, alpha subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MQDEKDTLRQQLLTLQSEALAQVEQADGLEALEQIRIDYLGKKGKLSKILGGMGKLPPEERPAIGALANTFKEALQSSLE
QRKQALGEAQLLARLEAERLDVTMPGPRRPLGRIHPLQATIDRMLDIFVGMGYTVVHGPELETEYYNFEALNTPADHPAR
DMQDTLYLANGYLMRTQTSSVQVRYMEQHQPPLRIVHAGRVMRRDTVDATHSAVFHQMEILAVGENLTFSDLKGTLNTFI
TRLLGKRETRFRPSYFPFTEPSAEMDVRFGSKWLEIFGCGMVDPNVFQAVGYDPERYTGFAAGIGIERMAMLLHGIQDIR
LFYTSDLRFLSQF

Sequences:

>Translated_333_residues
MQDEKDTLRQQLLTLQSEALAQVEQADGLEALEQIRIDYLGKKGKLSKILGGMGKLPPEERPAIGALANTFKEALQSSLE
QRKQALGEAQLLARLEAERLDVTMPGPRRPLGRIHPLQATIDRMLDIFVGMGYTVVHGPELETEYYNFEALNTPADHPAR
DMQDTLYLANGYLMRTQTSSVQVRYMEQHQPPLRIVHAGRVMRRDTVDATHSAVFHQMEILAVGENLTFSDLKGTLNTFI
TRLLGKRETRFRPSYFPFTEPSAEMDVRFGSKWLEIFGCGMVDPNVFQAVGYDPERYTGFAAGIGIERMAMLLHGIQDIR
LFYTSDLRFLSQF
>Mature_333_residues
MQDEKDTLRQQLLTLQSEALAQVEQADGLEALEQIRIDYLGKKGKLSKILGGMGKLPPEERPAIGALANTFKEALQSSLE
QRKQALGEAQLLARLEAERLDVTMPGPRRPLGRIHPLQATIDRMLDIFVGMGYTVVHGPELETEYYNFEALNTPADHPAR
DMQDTLYLANGYLMRTQTSSVQVRYMEQHQPPLRIVHAGRVMRRDTVDATHSAVFHQMEILAVGENLTFSDLKGTLNTFI
TRLLGKRETRFRPSYFPFTEPSAEMDVRFGSKWLEIFGCGMVDPNVFQAVGYDPERYTGFAAGIGIERMAMLLHGIQDIR
LFYTSDLRFLSQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=227, Percent_Identity=34.8017621145374, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4758340, Length=256, Percent_Identity=32.8125, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=51.3846153846154, Blast_Score=335, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI32566635, Length=288, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=262, Percent_Identity=29.7709923664122, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32563657, Length=262, Percent_Identity=29.7709923664122, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=258, Percent_Identity=30.6201550387597, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=86, Percent_Identity=44.1860465116279, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137424, Length=287, Percent_Identity=30.3135888501742, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI18858079, Length=275, Percent_Identity=29.8181818181818, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37699; Mature: 37699

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDEKDTLRQQLLTLQSEALAQVEQADGLEALEQIRIDYLGKKGKLSKILGGMGKLPPEE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCC
RPAIGALANTFKEALQSSLEQRKQALGEAQLLARLEAERLDVTMPGPRRPLGRIHPLQAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCHHHHH
IDRMLDIFVGMGYTVVHGPELETEYYNFEALNTPADHPARDMQDTLYLANGYLMRTQTSS
HHHHHHHHHCCCEEEEECCCCCCCEECEEECCCCCCCCHHHHHHHEEHHCCEEEEECCCC
VQVRYMEQHQPPLRIVHAGRVMRRDTVDATHSAVFHQMEILAVGENLTFSDLKGTLNTFI
EEEEEHHHCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHH
TRLLGKRETRFRPSYFPFTEPSAEMDVRFGSKWLEIFGCGMVDPNVFQAVGYDPERYTGF
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHCCH
AAGIGIERMAMLLHGIQDIRLFYTSDLRFLSQF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDEKDTLRQQLLTLQSEALAQVEQADGLEALEQIRIDYLGKKGKLSKILGGMGKLPPEE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCC
RPAIGALANTFKEALQSSLEQRKQALGEAQLLARLEAERLDVTMPGPRRPLGRIHPLQAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCHHHHH
IDRMLDIFVGMGYTVVHGPELETEYYNFEALNTPADHPARDMQDTLYLANGYLMRTQTSS
HHHHHHHHHCCCEEEEECCCCCCCEECEEECCCCCCCCHHHHHHHEEHHCCEEEEECCCC
VQVRYMEQHQPPLRIVHAGRVMRRDTVDATHSAVFHQMEILAVGENLTFSDLKGTLNTFI
EEEEEHHHCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHH
TRLLGKRETRFRPSYFPFTEPSAEMDVRFGSKWLEIFGCGMVDPNVFQAVGYDPERYTGF
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHCCH
AAGIGIERMAMLLHGIQDIRLFYTSDLRFLSQF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]