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The map label for this gene is lpdA [H]

Identifier: 86608233

GI number: 86608233

Start: 772180

End: 773562

Strand: Direct

Name: lpdA [H]

Synonym: CYB_0749

Alternate gene names: 86608233

Gene position: 772180-773562 (Clockwise)

Preceding gene: 86608219

Following gene: 86608234

Centisome position: 25.34

GC content: 62.11

Gene sequence:

>1383_bases
ATGAGCTTCGATTTTGACTTGATCATCATCGGTGCCGGCGTCGGCGGTCACGGCGCTGCTTTGCATGCTGTCGAATCCGG
CCTGAAAACCGCCATCGTCGAAGGTGCAGAAATGGGCGGCACCTGCATCAACCGCGGCTGCATCCCCTCCAAGGCTCTGC
TGGCCGCCTCAGGACGCCTGCGGGAACTGCAACATAGCTCTGGGCTGGGCATCCAAGTGGGATCCCTTCAGGTGAACCGA
GAGGCTATTGCCAATCATGCTGCCCAAGTGGTGGAGAAGATCCGCGCCGACATGACTCGCTCGCTGGAGAAGCTGGGGGT
AACAATCCTGAGAGGGCGAGGCAAGTTGGTGGCACCCCAGCAGGTGGAAGTCCAGGAAGAGAAGGGATCCCACACCTACA
CGGCACAGGATGTCATTCTCGCGACGGGCTCCCGCCCCTTTGTGCCCCCCGGCATCGAGGTGGATGGGCGGACGGTGTTT
ACCAGCGACGAGGCGGTGCGGCTGGAGTGGATCCCGGAGCGGCTGGCCATTATCGGCAGCGGCTACATCGGCCAGGAGTT
TGCCGACATCTACACCGCCCTGGGATCCCAGGTAATCCTCATCGAAGCCCTAGAGACCCTGATGCCGGCTTTCGACCCAG
ACATTGCCCGCCTGGCCCAGCGAGTGCTGATCAAGCCCCGCTCCATTCAAACCTTTGTGGGCGTGCTGGCTCGGCAGGTG
ATCCCCGGCCAGCCGGTGACCATTCACCTGTCCAACGGGGAGACGCTGCAAGTAGATGGCTGCCTGGTGGCTGCCGGTCG
GATCCCGGTCTCAGAGGGGCTGGGCTTGGCCGAGTTGGGCATCGACACCGGCAAACGGGGGTTTATTCCTGTCGATAGCC
GCATGGCCACCGATTTGCCTCACCTCTGGGCTATTGGGGATGTGACCGGCAAAATGATGTTGGCTCACGCGGCGGCAGCC
CAGGGGAGAGTGGCCGTGGAAAACATCTGTGGCCGTACCGCCTACATGGACTACCTCAGCATCCCGGCAGCGGTGTTCAC
TCACCCGGAAATGGGTTTTGTAGGGCTGACCGAGCCTCAGGCCAAGGAAGAAGGCTACAGCGTGGGCACAGTGCGAACCT
ACTTTGGCGGCAACTCCAAGGCCATTGCTTCGGGAGAGACAGAGGGCATGGTCAAGCTGGTCTTTGACAAGAGCACCGGC
CTACTGCTGGGATCCCATATCTTCGGGCCCCATGCGGCGGATTTGATCCACGAGGCGGCTCAGGCCATTGCTCGCCGGGC
CACGGTGCGAGAACTGGCCGGCTTGGTGCACGTTCACCCCACCTTGGCAGAGACGCTGGAGGAAGCCTACAAGCGGGCAA
CTCACGCTCTGGCAGGGGCTTAG

Upstream 100 bases:

>100_bases
CAAGCGACCCTATGCTACCCCATGGTTTGGGTGCTCTCAGCCTGGTTTCTGGGAAGGCCGACAAGATGAGAGAATGAAGC
ACCGGTTATCCAATCTCGCC

Downstream 100 bases:

>100_bases
CAACGGTTGGGAAGATCTCCCCGCTCAAAACCTCAAGAAAACCGTAGGATGGGGGGGAACGCGTGGGAGGAAGTGCTTTT
GGTGAGCGAAGCCGAGATTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSFDFDLIIIGAGVGGHGAALHAVESGLKTAIVEGAEMGGTCINRGCIPSKALLAASGRLRELQHSSGLGIQVGSLQVNR
EAIANHAAQVVEKIRADMTRSLEKLGVTILRGRGKLVAPQQVEVQEEKGSHTYTAQDVILATGSRPFVPPGIEVDGRTVF
TSDEAVRLEWIPERLAIIGSGYIGQEFADIYTALGSQVILIEALETLMPAFDPDIARLAQRVLIKPRSIQTFVGVLARQV
IPGQPVTIHLSNGETLQVDGCLVAAGRIPVSEGLGLAELGIDTGKRGFIPVDSRMATDLPHLWAIGDVTGKMMLAHAAAA
QGRVAVENICGRTAYMDYLSIPAAVFTHPEMGFVGLTEPQAKEEGYSVGTVRTYFGGNSKAIASGETEGMVKLVFDKSTG
LLLGSHIFGPHAADLIHEAAQAIARRATVRELAGLVHVHPTLAETLEEAYKRATHALAGA

Sequences:

>Translated_460_residues
MSFDFDLIIIGAGVGGHGAALHAVESGLKTAIVEGAEMGGTCINRGCIPSKALLAASGRLRELQHSSGLGIQVGSLQVNR
EAIANHAAQVVEKIRADMTRSLEKLGVTILRGRGKLVAPQQVEVQEEKGSHTYTAQDVILATGSRPFVPPGIEVDGRTVF
TSDEAVRLEWIPERLAIIGSGYIGQEFADIYTALGSQVILIEALETLMPAFDPDIARLAQRVLIKPRSIQTFVGVLARQV
IPGQPVTIHLSNGETLQVDGCLVAAGRIPVSEGLGLAELGIDTGKRGFIPVDSRMATDLPHLWAIGDVTGKMMLAHAAAA
QGRVAVENICGRTAYMDYLSIPAAVFTHPEMGFVGLTEPQAKEEGYSVGTVRTYFGGNSKAIASGETEGMVKLVFDKSTG
LLLGSHIFGPHAADLIHEAAQAIARRATVRELAGLVHVHPTLAETLEEAYKRATHALAGA
>Mature_459_residues
SFDFDLIIIGAGVGGHGAALHAVESGLKTAIVEGAEMGGTCINRGCIPSKALLAASGRLRELQHSSGLGIQVGSLQVNRE
AIANHAAQVVEKIRADMTRSLEKLGVTILRGRGKLVAPQQVEVQEEKGSHTYTAQDVILATGSRPFVPPGIEVDGRTVFT
SDEAVRLEWIPERLAIIGSGYIGQEFADIYTALGSQVILIEALETLMPAFDPDIARLAQRVLIKPRSIQTFVGVLARQVI
PGQPVTIHLSNGETLQVDGCLVAAGRIPVSEGLGLAELGIDTGKRGFIPVDSRMATDLPHLWAIGDVTGKMMLAHAAAAQ
GRVAVENICGRTAYMDYLSIPAAVFTHPEMGFVGLTEPQAKEEGYSVGTVRTYFGGNSKAIASGETEGMVKLVFDKSTGL
LLGSHIFGPHAADLIHEAAQAIARRATVRELAGLVHVHPTLAETLEEAYKRATHALAGA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=35.2941176470588, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI50301238, Length=468, Percent_Identity=30.1282051282051, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI148277065, Length=476, Percent_Identity=28.1512605042017, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI148277071, Length=476, Percent_Identity=28.1512605042017, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI33519430, Length=476, Percent_Identity=28.1512605042017, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI33519428, Length=476, Percent_Identity=28.1512605042017, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI33519426, Length=476, Percent_Identity=28.1512605042017, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI291045266, Length=477, Percent_Identity=27.0440251572327, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI22035672, Length=470, Percent_Identity=28.936170212766, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI291045268, Length=472, Percent_Identity=25.2118644067797, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI21389617, Length=219, Percent_Identity=29.2237442922374, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1786307, Length=444, Percent_Identity=34.6846846846847, Blast_Score=264, Evalue=9e-72,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=31.8082788671024, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=31.0904872389791, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=26.9736842105263, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=36.2255965292842, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71983429, Length=457, Percent_Identity=28.6652078774617, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=457, Percent_Identity=28.6652078774617, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=25.7861635220126, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=25.6728778467909, Blast_Score=104, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=34.7368421052632, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=29.0322580645161, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=28.2700421940928, Blast_Score=170, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=38.0434782608696, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=28.1573498964803, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24640553, Length=481, Percent_Identity=28.2744282744283, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=28.2744282744283, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=26.875, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48617; Mature: 48485

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDFDLIIIGAGVGGHGAALHAVESGLKTAIVEGAEMGGTCINRGCIPSKALLAASGRL
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHCCHH
RELQHSSGLGIQVGSLQVNREAIANHAAQVVEKIRADMTRSLEKLGVTILRGRGKLVAPQ
HHHHCCCCCCEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
QVEVQEEKGSHTYTAQDVILATGSRPFVPPGIEVDGRTVFTSDEAVRLEWIPERLAIIGS
CCEEHHCCCCCEEEECEEEEECCCCCCCCCCCEECCCEEEECCCEEEEEECHHHHHHCCC
GYIGQEFADIYTALGSQVILIEALETLMPAFDPDIARLAQRVLIKPRSIQTFVGVLARQV
CCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IPGQPVTIHLSNGETLQVDGCLVAAGRIPVSEGLGLAELGIDTGKRGFIPVDSRMATDLP
CCCCCEEEEECCCCEEEECCEEEEECCCCHHHCCCHHHHCCCCCCCCCEECCCHHHCCCC
HLWAIGDVTGKMMLAHAAAAQGRVAVENICGRTAYMDYLSIPAAVFTHPEMGFVGLTEPQ
CCEEEHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCHHHHCCCCCCEEECCCCC
AKEEGYSVGTVRTYFGGNSKAIASGETEGMVKLVFDKSTGLLLGSHIFGPHAADLIHEAA
HHHCCCCCCEEEEEECCCCCEEECCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHH
QAIARRATVRELAGLVHVHPTLAETLEEAYKRATHALAGA
HHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFDFDLIIIGAGVGGHGAALHAVESGLKTAIVEGAEMGGTCINRGCIPSKALLAASGRL
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHCCHH
RELQHSSGLGIQVGSLQVNREAIANHAAQVVEKIRADMTRSLEKLGVTILRGRGKLVAPQ
HHHHCCCCCCEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
QVEVQEEKGSHTYTAQDVILATGSRPFVPPGIEVDGRTVFTSDEAVRLEWIPERLAIIGS
CCEEHHCCCCCEEEECEEEEECCCCCCCCCCCEECCCEEEECCCEEEEEECHHHHHHCCC
GYIGQEFADIYTALGSQVILIEALETLMPAFDPDIARLAQRVLIKPRSIQTFVGVLARQV
CCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IPGQPVTIHLSNGETLQVDGCLVAAGRIPVSEGLGLAELGIDTGKRGFIPVDSRMATDLP
CCCCCEEEEECCCCEEEECCEEEEECCCCHHHCCCHHHHCCCCCCCCCEECCCHHHCCCC
HLWAIGDVTGKMMLAHAAAAQGRVAVENICGRTAYMDYLSIPAAVFTHPEMGFVGLTEPQ
CCEEEHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCHHHHCCCCCCEEECCCCC
AKEEGYSVGTVRTYFGGNSKAIASGETEGMVKLVFDKSTGLLLGSHIFGPHAADLIHEAA
HHHCCCCCCEEEEEECCCCCEEECCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHH
QAIARRATVRELAGLVHVHPTLAETLEEAYKRATHALAGA
HHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]