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The map label for this gene is ndhF [H]

Identifier: 86607617

GI number: 86607617

Start: 128766

End: 130922

Strand: Reverse

Name: ndhF [H]

Synonym: CYB_0115

Alternate gene names: 86607617

Gene position: 130922-128766 (Counterclockwise)

Preceding gene: 86607620

Following gene: 86607616

Centisome position: 4.3

GC content: 58.69

Gene sequence:

>2157_bases
ATGGAATTCATCTACACGTACGCCTGGTTGATCCCCGTCTATCCTTTGGTGGCAGCCTTTCTCCTCGGCTTAGGTATCCT
GATGTTCAACGCCTGGACAAGGGCCAACCGCTCTTTGGCTGCCTTTTTGAGCGTGTTCGCGACCGGGACGGCGATGGTTC
ATGCCTTTGCCCTTTTGGTCAGCCAGGTGCAGGGGCACGAAGCCGTCCTCAGAACCATCACCTGGGCCCAGGCGGGATCC
TTCTCGGTGGATATGGGCTTTGTCGTGGATCACCTGAGCGCCATGATGCTGGTGGTGGTGACCACGGTGGCCTTTCTGGT
GCAGATCTACTCCGATGGCTACATGGCCCACGATCCCGGCTATGTGCGTTTCTACGCCTACCTGAGCTTATTTAGCTCCT
CGATGCTGGGCTTGGTGGTCAGCCCCAACCTGGTGCAGATCTACATCTTCTGGGAGCTGGTGGGGATGTGCTCCTACCTG
CTCATTGGCTTTTGGCACGACCGGCAGGCAGCGGCAGAAGCAGCGCAAAAGGCGTTTGTGGTCAACCGGGTGGGAGATTT
CGGCCTGCTGTTGGGCATCTTAGGTTTTTACTGGATTTCCGGTACCTTCGCGTTTGACGAGATCGGGCGCAACTTGCAGG
AGATGTTGGCTGCCGGAGCGGTGAGCAGCAGCTTGATTGCCCTGTTTGCGGTGTTGGTGTTCTTGGGGCCGGTGGCCAAG
TCGGCCCAGTTCCCGCTGCATGTGTGGTTGCCGGATGCTATGGAAGGTCCCACGCCTATCTCGGCTCTCATCCACGCAGC
CACGATGGTGGCGGCTGGGGTATTCCTCTTGGCCCGCATGTTCCCGGTGCTGGAAGGGATCCCGGCGGCCATGGACATCA
TCGCCTGGACAGGGGCGGGCACAGCCTTCTTGGGGGCCACCATTGCCATCACCCAAAACGACATCAAAAAAGGCTTGGCC
TACTCCACCATTTCCCAGCTGGGCTACATGGTCATGGCCATGGGGTGCGGCGCCTATGGGGCGGGCCTGTTTCACCTGAT
GACCCACGCCTACTTTAAGGCGATGCTCTTTTTGTGCTCTGGCTCAGTTATCCACGGCATGGAAGCGGTGGTCGGCCATG
ACCCGGTTTTGGCCCAAGATATGCGCCTGATGGGGGGGTTGCGCAAATACATGCCGGTTACGGCCATCACGTTTTTGATC
GGCACGTTGGCCATTTGTGGGATCCCGCCCTTCGCCGGCTTTTGGTCTAAAGACGAGATCTTGGGCGCTGTTTTCGAACG
GAACCCAGCTCTGTGGGGGGTGGGCTTCCTCACCGCCGGCATCACTGCCTTCTACATGTTTCGCATCTATTTCCTCACCT
TTGAAGGGGAGTTTCGCGGCACCAACCTGAGACTGCGGCAGGAGGTGGCCATCAGCCAGGGCCGTCCTGTGCCAGCTTTT
GCCTACGTTTTTGCGGGGAAACAACCGGCCTTTGGCCCTGGAGCCATGAACATTCAAGAAAAACCCCAGCCCAGCAGCCA
TCCTGAAGCCCACGAAAACCTGCACGACGGAGATGCCCATGCCTCAGAGCCCCATGAGTCCCCTTGGTCGATGACCTTCC
CGCTGTTGGCTCTGGCCGTCCCTTCTGTGCTGATTGGCCTGGTGGGTACCCCTTTTGCCAACTTCTTTGAAGCTTTCATC
CACCCACCGACTGAGTCTGTGGCAGAGGCAGGCTCGATTTTGCCGCTGGTGGCAGCAGAGTGGCAGGAGGTGCTGGCGGG
CCATTTCGATTGGGCAGAGTTTCTAATCATGGCGGGTAGCTCCGTGGGCATTGGGCTGATCGGCATCACCTTGGCCAGCT
TGATCTACTGGCAAAAGCGCATCGACCCGGGCACCTTCCCCCAAAGCCTGCAACCGCTCTACTACTTCTCCCTCAACAAG
TGGTACATTGACGACCTCTATGAGCGAGTCTTTGTGCAGGGCACCCGCTTCGTGGCACGGGAGGCGCTGGAGGTGGACAG
CCGCATCGTGGATGGTGTGGTCAACCTGACGGGCTTGGTGACCATGGTGAGCGGCGAGGCGATGAAATACCTGGAGAGCG
GCAAGGCCCAGTTTTACGCTTTGATTATCTTTGCAGCGGTGGTGGTGATGGTGGTGGCATCAGGGGTAGGGTTCTAG

Upstream 100 bases:

>100_bases
GTTGAGCAAATGCCAGCCCACCAGACACTCCCACCGGCCAATTGCAGGGCTAGCAGAGCAGGTTCGTTGCTGTGAGAGGA
CGCATTCAGAAGCATTTCCC

Downstream 100 bases:

>100_bases
CTCGAAGGCGAGGATGGATCCGGCCCGCTTGGCCAAGGGATCCCTCAGCCTAGAACTGCTGCTATGCTACGGAGCAAGTT
GAGCGGATCCCTTGTTGCAA

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MEFIYTYAWLIPVYPLVAAFLLGLGILMFNAWTRANRSLAAFLSVFATGTAMVHAFALLVSQVQGHEAVLRTITWAQAGS
FSVDMGFVVDHLSAMMLVVVTTVAFLVQIYSDGYMAHDPGYVRFYAYLSLFSSSMLGLVVSPNLVQIYIFWELVGMCSYL
LIGFWHDRQAAAEAAQKAFVVNRVGDFGLLLGILGFYWISGTFAFDEIGRNLQEMLAAGAVSSSLIALFAVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLLARMFPVLEGIPAAMDIIAWTGAGTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGCGAYGAGLFHLMTHAYFKAMLFLCSGSVIHGMEAVVGHDPVLAQDMRLMGGLRKYMPVTAITFLI
GTLAICGIPPFAGFWSKDEILGAVFERNPALWGVGFLTAGITAFYMFRIYFLTFEGEFRGTNLRLRQEVAISQGRPVPAF
AYVFAGKQPAFGPGAMNIQEKPQPSSHPEAHENLHDGDAHASEPHESPWSMTFPLLALAVPSVLIGLVGTPFANFFEAFI
HPPTESVAEAGSILPLVAAEWQEVLAGHFDWAEFLIMAGSSVGIGLIGITLASLIYWQKRIDPGTFPQSLQPLYYFSLNK
WYIDDLYERVFVQGTRFVAREALEVDSRIVDGVVNLTGLVTMVSGEAMKYLESGKAQFYALIIFAAVVVMVVASGVGF

Sequences:

>Translated_718_residues
MEFIYTYAWLIPVYPLVAAFLLGLGILMFNAWTRANRSLAAFLSVFATGTAMVHAFALLVSQVQGHEAVLRTITWAQAGS
FSVDMGFVVDHLSAMMLVVVTTVAFLVQIYSDGYMAHDPGYVRFYAYLSLFSSSMLGLVVSPNLVQIYIFWELVGMCSYL
LIGFWHDRQAAAEAAQKAFVVNRVGDFGLLLGILGFYWISGTFAFDEIGRNLQEMLAAGAVSSSLIALFAVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLLARMFPVLEGIPAAMDIIAWTGAGTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGCGAYGAGLFHLMTHAYFKAMLFLCSGSVIHGMEAVVGHDPVLAQDMRLMGGLRKYMPVTAITFLI
GTLAICGIPPFAGFWSKDEILGAVFERNPALWGVGFLTAGITAFYMFRIYFLTFEGEFRGTNLRLRQEVAISQGRPVPAF
AYVFAGKQPAFGPGAMNIQEKPQPSSHPEAHENLHDGDAHASEPHESPWSMTFPLLALAVPSVLIGLVGTPFANFFEAFI
HPPTESVAEAGSILPLVAAEWQEVLAGHFDWAEFLIMAGSSVGIGLIGITLASLIYWQKRIDPGTFPQSLQPLYYFSLNK
WYIDDLYERVFVQGTRFVAREALEVDSRIVDGVVNLTGLVTMVSGEAMKYLESGKAQFYALIIFAAVVVMVVASGVGF
>Mature_718_residues
MEFIYTYAWLIPVYPLVAAFLLGLGILMFNAWTRANRSLAAFLSVFATGTAMVHAFALLVSQVQGHEAVLRTITWAQAGS
FSVDMGFVVDHLSAMMLVVVTTVAFLVQIYSDGYMAHDPGYVRFYAYLSLFSSSMLGLVVSPNLVQIYIFWELVGMCSYL
LIGFWHDRQAAAEAAQKAFVVNRVGDFGLLLGILGFYWISGTFAFDEIGRNLQEMLAAGAVSSSLIALFAVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLLARMFPVLEGIPAAMDIIAWTGAGTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGCGAYGAGLFHLMTHAYFKAMLFLCSGSVIHGMEAVVGHDPVLAQDMRLMGGLRKYMPVTAITFLI
GTLAICGIPPFAGFWSKDEILGAVFERNPALWGVGFLTAGITAFYMFRIYFLTFEGEFRGTNLRLRQEVAISQGRPVPAF
AYVFAGKQPAFGPGAMNIQEKPQPSSHPEAHENLHDGDAHASEPHESPWSMTFPLLALAVPSVLIGLVGTPFANFFEAFI
HPPTESVAEAGSILPLVAAEWQEVLAGHFDWAEFLIMAGSSVGIGLIGITLASLIYWQKRIDPGTFPQSLQPLYYFSLNK
WYIDDLYERVFVQGTRFVAREALEVDSRIVDGVVNLTGLVTMVSGEAMKYLESGKAQFYALIIFAAVVVMVVASGVGF

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=457, Percent_Identity=36.1050328227571, Blast_Score=265, Evalue=8e-71,
Organism=Escherichia coli, GI1788614, Length=442, Percent_Identity=43.2126696832579, Blast_Score=300, Evalue=3e-82,
Organism=Escherichia coli, GI1788829, Length=393, Percent_Identity=31.8066157760814, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1788831, Length=374, Percent_Identity=29.9465240641711, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1788827, Length=427, Percent_Identity=28.8056206088993, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788613, Length=349, Percent_Identity=26.647564469914, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI2367154, Length=398, Percent_Identity=23.6180904522613, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=458, Percent_Identity=26.4192139737991, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 77993; Mature: 77993

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]