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The map label for this gene is tig

Identifier: 86607584

GI number: 86607584

Start: 96348

End: 98018

Strand: Reverse

Name: tig

Synonym: CYB_0082

Alternate gene names: 86607584

Gene position: 98018-96348 (Counterclockwise)

Preceding gene: 86607585

Following gene: 86607582

Centisome position: 3.22

GC content: 57.51

Gene sequence:

>1671_bases
ATGCAGGTCACACAAGAAAAACGCCCTGGCAGCCGGGTTGGCCTCAAAATCGTGGTAGAAGCCGACCGGGTGAAGCGCTC
CTACGAAAAAACCTTGCGTCAACTGGAGCAGAATATCCAAATTCCGGGATTCCGCAAGGGCAAGGCCCCCCGCAATCTTG
TTGTGCGGCAGGTGGGCCGCGAGCGCATCCTGGCCAGTGCTGTCGATGACCTAATCAACGAGGCCATTCAACAGGCTTTC
AAGGAAGCTCAGCTCACCCCCATCAGTCGGTTCGAGCTGGATGACGAGGTGGGGCAGCTCTTGGCTCAGTTCAACCCCGA
AGCAGACTTCAGCTTCTCAGGATATGTTGAGGTGTACCCCGAAGCCCGCGTTGGCCAATACAAAGGGCTGACCGTTACGG
CAACCCGTGTTGACGTCAAGCCTGAGCAAATTGACCAGCTCATCGACCGATGGCGGGATCAGCGGGCCACCCTCTTGCCG
GTAGAAGATCGGCCTGCTCAGTTGGGGGATGTGGTGGTGATCGATTTTGCAGCCCGCGATGCAGAAGGCAATCCCCTCGA
CGAGATGACCACCCAGGACTTCCAGCTGGAACTCAAGGAAGACAACTTCATCCCCGGTTTTGTGGCGGGCATCGTGGGCA
TGCAGCTCGACGAAACCAAGGAGATCGCAGCCACCTTCCCCGACGACTACTTCAGAAAAGAGTTGGCCGGGAAAACGGTT
ACCTTCATCGTCTGCCTGAGGGAGATCAAGGCCAAAGAGCTGCCCGAATTGGATGATGCCTTTGTGCAAGAGATCAGCAG
CTTCCAAACGGTAGCCGAGTTGCGGGAACACCTGCAAAAGCGCCTCGAACAGGATGCCCTGAGACAATCCGAGGAGAACC
TGGAAACCGCCATCCTCAACGCCATCTTGGAGACTACTGAGGTGGATTTGCCGGAAACCCTCATCGAACAAGAAACGACA
CAACTGCTGGCCCAGAGTCTCCAGGCCCTGCGGCAGGAAGGCATCAAGCCGGGGGAGCTCCGTAAGTTCTTGTCCGAGTT
GCCTCCCGAAACCCTCAACCAGTTGAAAGAGCGCCACCGACCCGAAGCCATCGACCGGCTGCGGCGCACCTTGGCCCTGA
GCGCCATCGTTCGGCAAGAGCAAATTGCCGTAGGTTCCACGGAGTTGGACGTGGAGGTGGAAGAGGTGATGGCGGCCTAT
GCCCAGCAAGGCCAAAAGCTGGATCGGGAACGAGTACGCCAAGCCGTCTATGAAAGCTTGCTGACCAACAAGGTGATGGC
TTGGCTGAAGTCTCAGACCACAGTCAACTGGGTCGATAGCGAGGGGAATCCAACCCAGGCGCCCACTTCTTTGGCAGGCT
CGGAGGAGAAGGTTGAAGTGCCAGAGGCCGAGTTTGAGGCCGATGAGCCAGAAGCAGAGGTATCTGGGATCCCTGAGGCA
GTTGAATCCTCATCAGAGACGGCTACGGGGGCGGAAACGGATGGAGAAGCTGCAGCTGCGGAAGCCGAGCCAGCTACAGA
AAAGGCTGTAGAAGCATCCCCTGCTGAAACCGTCTCAGCCTCTGCGGCAGAGGCAACGCTCCCAGTTGAGGAGAAAGCGG
CAGAGACCGCTACAGAGATCCCGGCAGCAGAAAAGCCCAAGCCAAGCAAAAAGGACAAAAAAGGCAAGTAA

Upstream 100 bases:

>100_bases
AAGTGGCCACTGAGGGATCCCCTGCCTAGAATCTCCAGGTTGACCCACAGGCCGTTGAGGTAGAATTGAACTTCTGCTTG
CCCACTAGCTCATACTTCCT

Downstream 100 bases:

>100_bases
ACTACCCGACACCCGCGCTACGCGCAGGGTGCGGGCTTTATCCCAATCCCCAGCGGCCCCAGAGGACAGCTAAGTTTCGG
AATTTCAGGCGAGTTTACAG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MQVTQEKRPGSRVGLKIVVEADRVKRSYEKTLRQLEQNIQIPGFRKGKAPRNLVVRQVGRERILASAVDDLINEAIQQAF
KEAQLTPISRFELDDEVGQLLAQFNPEADFSFSGYVEVYPEARVGQYKGLTVTATRVDVKPEQIDQLIDRWRDQRATLLP
VEDRPAQLGDVVVIDFAARDAEGNPLDEMTTQDFQLELKEDNFIPGFVAGIVGMQLDETKEIAATFPDDYFRKELAGKTV
TFIVCLREIKAKELPELDDAFVQEISSFQTVAELREHLQKRLEQDALRQSEENLETAILNAILETTEVDLPETLIEQETT
QLLAQSLQALRQEGIKPGELRKFLSELPPETLNQLKERHRPEAIDRLRRTLALSAIVRQEQIAVGSTELDVEVEEVMAAY
AQQGQKLDRERVRQAVYESLLTNKVMAWLKSQTTVNWVDSEGNPTQAPTSLAGSEEKVEVPEAEFEADEPEAEVSGIPEA
VESSSETATGAETDGEAAAAEAEPATEKAVEASPAETVSASAAEATLPVEEKAAETATEIPAAEKPKPSKKDKKGK

Sequences:

>Translated_556_residues
MQVTQEKRPGSRVGLKIVVEADRVKRSYEKTLRQLEQNIQIPGFRKGKAPRNLVVRQVGRERILASAVDDLINEAIQQAF
KEAQLTPISRFELDDEVGQLLAQFNPEADFSFSGYVEVYPEARVGQYKGLTVTATRVDVKPEQIDQLIDRWRDQRATLLP
VEDRPAQLGDVVVIDFAARDAEGNPLDEMTTQDFQLELKEDNFIPGFVAGIVGMQLDETKEIAATFPDDYFRKELAGKTV
TFIVCLREIKAKELPELDDAFVQEISSFQTVAELREHLQKRLEQDALRQSEENLETAILNAILETTEVDLPETLIEQETT
QLLAQSLQALRQEGIKPGELRKFLSELPPETLNQLKERHRPEAIDRLRRTLALSAIVRQEQIAVGSTELDVEVEEVMAAY
AQQGQKLDRERVRQAVYESLLTNKVMAWLKSQTTVNWVDSEGNPTQAPTSLAGSEEKVEVPEAEFEADEPEAEVSGIPEA
VESSSETATGAETDGEAAAAEAEPATEKAVEASPAETVSASAAEATLPVEEKAAETATEIPAAEKPKPSKKDKKGK
>Mature_556_residues
MQVTQEKRPGSRVGLKIVVEADRVKRSYEKTLRQLEQNIQIPGFRKGKAPRNLVVRQVGRERILASAVDDLINEAIQQAF
KEAQLTPISRFELDDEVGQLLAQFNPEADFSFSGYVEVYPEARVGQYKGLTVTATRVDVKPEQIDQLIDRWRDQRATLLP
VEDRPAQLGDVVVIDFAARDAEGNPLDEMTTQDFQLELKEDNFIPGFVAGIVGMQLDETKEIAATFPDDYFRKELAGKTV
TFIVCLREIKAKELPELDDAFVQEISSFQTVAELREHLQKRLEQDALRQSEENLETAILNAILETTEVDLPETLIEQETT
QLLAQSLQALRQEGIKPGELRKFLSELPPETLNQLKERHRPEAIDRLRRTLALSAIVRQEQIAVGSTELDVEVEEVMAAY
AQQGQKLDRERVRQAVYESLLTNKVMAWLKSQTTVNWVDSEGNPTQAPTSLAGSEEKVEVPEAEFEADEPEAEVSGIPEA
VESSSETATGAETDGEAAAAEAEPATEKAVEASPAETVSASAAEATLPVEEKAAETATEIPAAEKPKPSKKDKKGK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=404, Percent_Identity=26.2376237623762, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_SYNJB (Q2JQ33)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_476346.1
- ProteinModelPortal:   Q2JQ33
- STRING:   Q2JQ33
- GeneID:   3900064
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0082
- TIGR:   CYB_0082
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   TERENIE
- ProtClustDB:   PRK01490
- BioCyc:   SSP321332:CYB_0082-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 61743; Mature: 61743

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTQEKRPGSRVGLKIVVEADRVKRSYEKTLRQLEQNIQIPGFRKGKAPRNLVVRQVGR
CCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ERILASAVDDLINEAIQQAFKEAQLTPISRFELDDEVGQLLAQFNPEADFSFSGYVEVYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECC
EARVGQYKGLTVTATRVDVKPEQIDQLIDRWRDQRATLLPVEDRPAQLGDVVVIDFAARD
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCC
AEGNPLDEMTTQDFQLELKEDNFIPGFVAGIVGMQLDETKEIAATFPDDYFRKELAGKTV
CCCCCHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCHH
TFIVCLREIKAKELPELDDAFVQEISSFQTVAELREHLQKRLEQDALRQSEENLETAILN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AILETTEVDLPETLIEQETTQLLAQSLQALRQEGIKPGELRKFLSELPPETLNQLKERHR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCC
PEAIDRLRRTLALSAIVRQEQIAVGSTELDVEVEEVMAAYAQQGQKLDRERVRQAVYESL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTNKVMAWLKSQTTVNWVDSEGNPTQAPTSLAGSEEKVEVPEAEFEADEPEAEVSGIPEA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHCCCCHH
VESSSETATGAETDGEAAAAEAEPATEKAVEASPAETVSASAAEATLPVEEKAAETATEI
HHCCCCCCCCCCCCCCHHCCCCCCHHHHHHCCCCHHHHHCCHHHCCCCCHHHHHHHHHHC
PAAEKPKPSKKDKKGK
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQVTQEKRPGSRVGLKIVVEADRVKRSYEKTLRQLEQNIQIPGFRKGKAPRNLVVRQVGR
CCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ERILASAVDDLINEAIQQAFKEAQLTPISRFELDDEVGQLLAQFNPEADFSFSGYVEVYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECC
EARVGQYKGLTVTATRVDVKPEQIDQLIDRWRDQRATLLPVEDRPAQLGDVVVIDFAARD
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCC
AEGNPLDEMTTQDFQLELKEDNFIPGFVAGIVGMQLDETKEIAATFPDDYFRKELAGKTV
CCCCCHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCHH
TFIVCLREIKAKELPELDDAFVQEISSFQTVAELREHLQKRLEQDALRQSEENLETAILN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AILETTEVDLPETLIEQETTQLLAQSLQALRQEGIKPGELRKFLSELPPETLNQLKERHR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCC
PEAIDRLRRTLALSAIVRQEQIAVGSTELDVEVEEVMAAYAQQGQKLDRERVRQAVYESL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTNKVMAWLKSQTTVNWVDSEGNPTQAPTSLAGSEEKVEVPEAEFEADEPEAEVSGIPEA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHCCCCHH
VESSSETATGAETDGEAAAAEAEPATEKAVEASPAETVSASAAEATLPVEEKAAETATEI
HHCCCCCCCCCCCCCCHHCCCCCCHHHHHHCCCCHHHHHCCHHHCCCCCHHHHHHHHHHC
PAAEKPKPSKKDKKGK
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA