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The map label for this gene is purD [H]

Identifier: 86607545

GI number: 86607545

Start: 54109

End: 55404

Strand: Reverse

Name: purD [H]

Synonym: CYB_0043

Alternate gene names: 86607545

Gene position: 55404-54109 (Counterclockwise)

Preceding gene: 86607546

Following gene: 86607544

Centisome position: 1.82

GC content: 62.35

Gene sequence:

>1296_bases
ATGCAGGCTCTGGTCATCGGATCTGGGGGGCGAGAACACGCTCTGGCCTGGAAGCTGTTGCAGTCTCCCCAGGTTACTCG
GATCTACTGTGCCCCCGGCAACGGCGGCATGCAGCAGCTGCCCCGCTGTCAATCCGTGGCTTTGGCGGCTACCGACATTG
AAGGCCTGGTGCGGTTTGCTACCGTACAAGGGATCCGCCTGACGGTGGTAGGGCCAGAGCTGCCCTTGGTGCAGGGGATC
GTGGACGCCTTCCAGGCAGCAGGGTTGCCCATTTTCGGGCCTAGCCGCGCCGCTGCCCAACTGGAAGGAAGCAAACTCTG
GGCTAAGCAATTGATGCAAGAGGCGGGGATCCCGACTGCTCGCGCTACCGGCTTTGCTGAGCTATCTGCCGCTGTGGCCT
ATCTCGACTCCCAGAGTTTCCCAGTGGTCATCAAAAAAGATGGACTGGCTGCCGGCAAGGGGGTAACCGTTGCCCAAAAC
CGGGAAGAAGCCGTGCAAGCCCTGGAAGCAGCCCTGGCCAAGCCGGGCGATCAGGTGCTGATCGAGGAGTTTATCCCCGG
CCAGGAGGTGTCGGTTTTGGCTCTAACCGATGGCCAGATCTGTGTGCCGCTGCCACCTGCCCAGGACTACAAGCGCCTTG
GCGAGGGCGATACTGGCCCCAACACCGGCGGTATGGGAGCTCATGCCCCTGCGGATCACCTGGTAACTCCAGAACTGTTG
GCCCGCATCCAAGCCCAGATCCTGGATCCCACCCTGGAGGCTTTGCAACGGCGGGGCATCCGCTACCAGGGGGTGATCTA
TGCCGGTTTGATGATCACGCCCAGTGGGGATCCCTACGTGTTGGAGTACAACTGCCGCTTTGGGGATCCAGAGGCTCAAG
TCCTCCTGCCCTTGCTGGAAAACCCTTTGGACGAGGTGCTGCTGGCCTGTATAGAAGGACGGCTGGCCCAAATCCAGCTC
AAATGGAAACCCGGCTATGCCGCCTGCGTGGTGATGGCAGCCCCAGGCTATCCCGATGCCTACGAGCGAGGGATCCCCGT
CTCCGGCTTGGATGCAGCTGCCACCACGGGGGCGTTGGTGTTTCATGCGGGTACCCGCCAGGCAGGCAAGCAGCTCCTGA
GCGATGGCGGGCGCGTTCTCTGTCTTACCGGCCAAGGGGCAACCCTATCCGAGGCCCTGCGCCAAGCCTACGCAGCTGTG
GAGCAAGTGCAGTTTCCCAAGGCCTACTATCGTTCTGACATCGGCAGCCACGCCCTGGGGCCTGCCGTCCGCCGAGGAGT
GATCCCTACCCCATAA

Upstream 100 bases:

>100_bases
GGAGTGCGCCAACACCCCAGAAATGGTACAAGAACATGGTCTAATGGGGGATGTTCGGAAAACAGGCAAGCTGGGTTTAG
AAACAGCGGAATAGCACACC

Downstream 100 bases:

>100_bases
AGCCATCCCTTGCCCTCTCAGCTGCAGCCGGATGGAAGAAGCCCCAGAGGGGTCGCTCCTCCAGGGCCCATAAAGCAGCT
TTGAAAAAAGCTTTGATGCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQALVIGSGGREHALAWKLLQSPQVTRIYCAPGNGGMQQLPRCQSVALAATDIEGLVRFATVQGIRLTVVGPELPLVQGI
VDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTARATGFAELSAAVAYLDSQSFPVVIKKDGLAAGKGVTVAQN
REEAVQALEAALAKPGDQVLIEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPELL
ARIQAQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLENPLDEVLLACIEGRLAQIQL
KWKPGYAACVVMAAPGYPDAYERGIPVSGLDAAATTGALVFHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAV
EQVQFPKAYYRSDIGSHALGPAVRRGVIPTP

Sequences:

>Translated_431_residues
MQALVIGSGGREHALAWKLLQSPQVTRIYCAPGNGGMQQLPRCQSVALAATDIEGLVRFATVQGIRLTVVGPELPLVQGI
VDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTARATGFAELSAAVAYLDSQSFPVVIKKDGLAAGKGVTVAQN
REEAVQALEAALAKPGDQVLIEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPELL
ARIQAQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLENPLDEVLLACIEGRLAQIQL
KWKPGYAACVVMAAPGYPDAYERGIPVSGLDAAATTGALVFHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAV
EQVQFPKAYYRSDIGSHALGPAVRRGVIPTP
>Mature_431_residues
MQALVIGSGGREHALAWKLLQSPQVTRIYCAPGNGGMQQLPRCQSVALAATDIEGLVRFATVQGIRLTVVGPELPLVQGI
VDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTARATGFAELSAAVAYLDSQSFPVVIKKDGLAAGKGVTVAQN
REEAVQALEAALAKPGDQVLIEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPELL
ARIQAQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLENPLDEVLLACIEGRLAQIQL
KWKPGYAACVVMAAPGYPDAYERGIPVSGLDAAATTGALVFHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAV
EQVQFPKAYYRSDIGSHALGPAVRRGVIPTP

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=44.9411764705882, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=44.9411764705882, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=44.9411764705882, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=44.9411764705882, Blast_Score=359, Evalue=2e-99,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=47.0588235294118, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=40.5152224824356, Blast_Score=304, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=40.9090909090909, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=46.4037122969838, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=46.4037122969838, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45350; Mature: 45350

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALVIGSGGREHALAWKLLQSPQVTRIYCAPGNGGMQQLPRCQSVALAATDIEGLVRFA
CCEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHCCCHHHHEEEHHHHHHHHHHH
TVQGIRLTVVGPELPLVQGIVDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTA
EECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCH
RATGFAELSAAVAYLDSQSFPVVIKKDGLAAGKGVTVAQNREEAVQALEAALAKPGDQVL
HHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
IEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPELL
HHHCCCCCCEEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
ARIQAQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLE
HHHHHHHCCHHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHH
NPLDEVLLACIEGRLAQIQLKWKPGYAACVVMAAPGYPDAYERGIPVSGLDAAATTGALV
CCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHCCEEE
FHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAVEQVQFPKAYYRSDIGSHALG
EECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
PAVRRGVIPTP
HHHHCCCCCCC
>Mature Secondary Structure
MQALVIGSGGREHALAWKLLQSPQVTRIYCAPGNGGMQQLPRCQSVALAATDIEGLVRFA
CCEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHCCCHHHHEEEHHHHHHHHHHH
TVQGIRLTVVGPELPLVQGIVDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTA
EECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCH
RATGFAELSAAVAYLDSQSFPVVIKKDGLAAGKGVTVAQNREEAVQALEAALAKPGDQVL
HHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
IEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPELL
HHHCCCCCCEEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
ARIQAQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLE
HHHHHHHCCHHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHH
NPLDEVLLACIEGRLAQIQLKWKPGYAACVVMAAPGYPDAYERGIPVSGLDAAATTGALV
CCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHCCEEE
FHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAVEQVQFPKAYYRSDIGSHALG
EECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
PAVRRGVIPTP
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]