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The map label for this gene is yneJ [H]

Identifier: 86361062

GI number: 86361062

Start: 372951

End: 373856

Strand: Reverse

Name: yneJ [H]

Synonym: RHE_PF00332

Alternate gene names: 86361062

Gene position: 373856-372951 (Counterclockwise)

Preceding gene: 86361064

Following gene: 86361061

Centisome position: 58.19

GC content: 63.13

Gene sequence:

>906_bases
TTGGACCTTTCGTCGATCGAGATATTCCTCGCCGTCGCCAGCGACCGCAGCGTTACCAAGGCCGCAAAGGCAGTCGGTCG
GGTGCCGTCCAACGTGACGACACGCATCCAGCAACTGGAGGAGGACCTTGGCGCTTGCCTGTTCAGCCGCGACGGCAAGA
AGATGACGCTGACTCGGGAGGGCGAGACGTTTCTCACCTATGCCAACCGGCTGATGGCGCTTGCGCTAGAAGCGCGCCGG
GCCGTGCGGCCTCTCGCCCCATCGGGAACATTACGGGTCGGCACGATGGAAAGCACGGCGGCAAGCCGCCTGCCGGCGGC
GCTGACGCAATTCAACCGGATGTGGCCGGATGTGTCGCTGCATCTGACAATGGGGGCTTCTCGCGATCTTGCCCGCGATG
TTCTGGCCGATGTGCTGGATTGCGCGCTGATTGCCCGCCCGCCGGAGACGATGCGCGAGGAGGATTCGGATTTCGATGCC
GAACTCATGGCGCTGGAGATGGAGACGGTTTTTGTCGAAGATCTGTTGATCGTGCTGCCATCCGGCCATCCCGCCATCAA
CTCCGCGGCCGACCTGCGGGTCGGATCGCTGGCAGCTTTGGAACCCGGCTGCACCTATCGCCGGATTGCCGAAAACTGGG
CGCGCAAATCGAACACCCTGCCGACGAGCGAGCTCGGTTCCTACCACGCGATCCTGGCGAGCGTTGCGACTGGGAATACG
GCGGGCGTGATGCCCAGGTCCGTTCTCGATCTCATGCACTGGCCGACCGCCGTTCATACCCATCAGCTCGGGCCGGTCGA
GACCCTGCTGGTCTATCGCAAGGACAATTGCCCCAGCGCTTTCAGGGCCTTTCATGAAGTGCTTGGCGCAACCAAAGGCA
GAGAGGTCAGGCTGGCGGCGAACTAG

Upstream 100 bases:

>100_bases
AGTCCGGGTTTTTGCCTGTGCCTAAACCCGCTTGAGCTCGGGTTATGATTTTTATATAGAGAACAATGACTCGCTTTAAT
TCCCGAAAAGAGATTTTTCA

Downstream 100 bases:

>100_bases
CTCCTGCCTCTCTTCCGTCACCCCCAGATTATCCTCACTCATAATTGGTCTTCGCATGCAGTTTCCCGTTTCACCGAAAG
TGGGGCCGAGCCGTATCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRIQQLEEDLGACLFSRDGKKMTLTREGETFLTYANRLMALALEARR
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNRMWPDVSLHLTMGASRDLARDVLADVLDCALIARPPETMREEDSDFDA
ELMALEMETVFVEDLLIVLPSGHPAINSAADLRVGSLAALEPGCTYRRIAENWARKSNTLPTSELGSYHAILASVATGNT
AGVMPRSVLDLMHWPTAVHTHQLGPVETLLVYRKDNCPSAFRAFHEVLGATKGREVRLAAN

Sequences:

>Translated_301_residues
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRIQQLEEDLGACLFSRDGKKMTLTREGETFLTYANRLMALALEARR
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNRMWPDVSLHLTMGASRDLARDVLADVLDCALIARPPETMREEDSDFDA
ELMALEMETVFVEDLLIVLPSGHPAINSAADLRVGSLAALEPGCTYRRIAENWARKSNTLPTSELGSYHAILASVATGNT
AGVMPRSVLDLMHWPTAVHTHQLGPVETLLVYRKDNCPSAFRAFHEVLGATKGREVRLAAN
>Mature_301_residues
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRIQQLEEDLGACLFSRDGKKMTLTREGETFLTYANRLMALALEARR
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNRMWPDVSLHLTMGASRDLARDVLADVLDCALIARPPETMREEDSDFDA
ELMALEMETVFVEDLLIVLPSGHPAINSAADLRVGSLAALEPGCTYRRIAENWARKSNTLPTSELGSYHAILASVATGNT
AGVMPRSVLDLMHWPTAVHTHQLGPVETLLVYRKDNCPSAFRAFHEVLGATKGREVRLAAN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=297, Percent_Identity=35.6902356902357, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790208, Length=262, Percent_Identity=30.1526717557252, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082132, Length=249, Percent_Identity=26.9076305220884, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI157672245, Length=181, Percent_Identity=28.7292817679558, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=229, Percent_Identity=26.6375545851528, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693105, Length=144, Percent_Identity=27.0833333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=216, Percent_Identity=23.6111111111111, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789173, Length=142, Percent_Identity=28.8732394366197, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32741; Mature: 32741

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRIQQLEEDLGACLFSRDGKKMTLTRE
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GETFLTYANRLMALALEARRAVRPLAPSGTLRVGTMESTAASRLPAALTQFNRMWPDVSL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEE
HLTMGASRDLARDVLADVLDCALIARPPETMREEDSDFDAELMALEMETVFVEDLLIVLP
EEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEC
SGHPAINSAADLRVGSLAALEPGCTYRRIAENWARKSNTLPTSELGSYHAILASVATGNT
CCCCCCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCCC
AGVMPRSVLDLMHWPTAVHTHQLGPVETLLVYRKDNCPSAFRAFHEVLGATKGREVRLAA
CCCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEC
N
C
>Mature Secondary Structure
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRIQQLEEDLGACLFSRDGKKMTLTRE
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GETFLTYANRLMALALEARRAVRPLAPSGTLRVGTMESTAASRLPAALTQFNRMWPDVSL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEE
HLTMGASRDLARDVLADVLDCALIARPPETMREEDSDFDAELMALEMETVFVEDLLIVLP
EEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEC
SGHPAINSAADLRVGSLAALEPGCTYRRIAENWARKSNTLPTSELGSYHAILASVATGNT
CCCCCCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCCC
AGVMPRSVLDLMHWPTAVHTHQLGPVETLLVYRKDNCPSAFRAFHEVLGATKGREVRLAA
CCCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]