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The map label for this gene is treZ [H]

Identifier: 86360993

GI number: 86360993

Start: 280757

End: 282550

Strand: Reverse

Name: treZ [H]

Synonym: RHE_PF00263

Alternate gene names: 86360993

Gene position: 282550-280757 (Counterclockwise)

Preceding gene: 86360994

Following gene: 86360992

Centisome position: 43.98

GC content: 60.65

Gene sequence:

>1794_bases
TTGACCAAAATGATATTCGGCCCCGCATTCACCGAAGATGGCGTTCTGTTTCGCCTTTGGGCTCCTCTGCATGAAAGCGT
TTCATTGAAAATCGAAGGCACTGATCCGCGCCCGATGCAAGCGGCTGAAGACGGCTGGCATCAATGCACGGTCGCGGACG
CGGGTCCCGGTACGCAGTATCGCTTCATCCTGCCGGACGGTCTTCAAATTCCCGATCCTGCCTCGCGCTTCCAGCCGCAG
GATGTGCATGGTGCAAGTGAAGTGGTCGACCTTGCTGCCTATCGCTGGAACACGCGCGAATGGACCGGCCGGCCCTGGGA
GGAGATGGTCATTTACGAACTGCATATCGGCTGCTTCACACCGCAGGGGACTTTCAAGGCCGCGATCGATCGTCTCGATC
ATCTGCGGGAACTGGGCGTCACGGCGCTGCAGATCATGCCGCTCAGCGACTTCCCCGGCCGCTATAACTGGGGCTATGAC
GGCGTGCTTCCCTATGCACCCGACAGCACTTACGGCCGGCCGGAAGATCTTATGGCGCTGGTGGATGCGGCGCACGAGCG
TAGCATCTCGGTTTTCCTCGACGCGGTCTACAACCACTTCGGACCCGACGGGAACTATATTCCCTCCTACGCGCCGCTCT
TTACCGATCATCACAGGACGCCCTGGGGCCACGGCATCAATTATGACGGCGACGGCTCTGAGATGATCCGCGAATTCTTC
ATCGAGAACGCCACCTATTGGATCAACGAGTTCAGGCTCGACGGTTTTCGCTTCGACGCCGTCCACGCCATCAAGGACGA
TAGCGCTGAGCATCTTCTTCACGCGCTGGCCCGCCGCGTCAGGGCTGCGGCCGGTGACCGGCATGTTCATCTGATCGTGG
AAAATGAGGCGAATGACAGCGACCTTCTGAAACGTGACGCAGACGGCGAGGCAAGGCTGTTCACGGCCCAATGGAACGAC
GATGTCCACCATGTGCTTCATATTACCGCCACGGGTGAAACCTTCGGTTACTATGCCGACTATGCAGGTGACGCCGGCAA
GCTCGGCAGGGCGCTGACGGAAGGCTTCGTGTTCCAGGGCGAGCACATGCCGTATCGCGGCCGAAGCCGAGGTCGGCCGA
GCGGCCATCTGCCGCCCACCGCCTTCGTGTCCTTTATCCAGAATCATGACCAGATCGGCAACCGGGCGCTGGGGGACCGG
ATTATGGCTTCGAGCCCGACCGACGCCGTCAAGGCTGTCACATCAATCTACCTGCTGGCACCGCAGATACCGATGCTGTT
CATGGGTGAGGAATGGGGTGCGCGAGAGCCTTTTCCGTTTTTCTGCGATTTCGACGAGAGCCTGAACGAGAAGGTCAGGA
AGGGCAGGCGCGAGGAGCTTTCGCGCCTGCCGGGTTTCGACTCTGACGAGCTTCTAGATCCGACGGCGCCGTCGACCTTT
GCGGCCGCCAAGCTCGACTGGTCGAAACGTACCTCGTCGGAAATGCTTGAATTCTACAGAATGCTTCTCGACCTCCGGCA
TCAAAGGATCGTGCCCCTGCTGAAGGGCGCCGGCGGCGGAAATGCGGTCTCTCGCTCCGCGGGTTCGACGCTTGTGGTGG
ATTGGGCCCTGGCCGGCGACCGGCGCCTTCATCTGGATGCCAATCTCGGCAGCGAGGCGGCGCCGCTTCTCCAGCAGCGC
AACGAAGGGGAGATGATCTTCCGCCTCGGCGGCAGCGATGGAGGCAAACTGGCGCCCTGGACGGTGATCTGGAGCATTTC
GAAAGGTGAGGGCATGAAGGCGATCGGACGATGA

Upstream 100 bases:

>100_bases
CGCTGACCAGGTCTCGCTTGCTTGACGCACCACCCAGGCGAAACTTTTTGCTCTGACAAAAGTTACTTAAATTCCGTCCT
AACGTCGCCGAAGGCTTCCC

Downstream 100 bases:

>100_bases
AATCTGCCGAGCTCGACAAGCTCGCCCGGCGATACGGGATCAGTCCGACCAGGCCCAGTCCCGAGGATCGTGAGGTGACG
ATTTCCGCCGACACCAAACG

Product: 1,4-alpha-glucan branching protein

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTKMIFGPAFTEDGVLFRLWAPLHESVSLKIEGTDPRPMQAAEDGWHQCTVADAGPGTQYRFILPDGLQIPDPASRFQPQ
DVHGASEVVDLAAYRWNTREWTGRPWEEMVIYELHIGCFTPQGTFKAAIDRLDHLRELGVTALQIMPLSDFPGRYNWGYD
GVLPYAPDSTYGRPEDLMALVDAAHERSISVFLDAVYNHFGPDGNYIPSYAPLFTDHHRTPWGHGINYDGDGSEMIREFF
IENATYWINEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEANDSDLLKRDADGEARLFTAQWND
DVHHVLHITATGETFGYYADYAGDAGKLGRALTEGFVFQGEHMPYRGRSRGRPSGHLPPTAFVSFIQNHDQIGNRALGDR
IMASSPTDAVKAVTSIYLLAPQIPMLFMGEEWGAREPFPFFCDFDESLNEKVRKGRREELSRLPGFDSDELLDPTAPSTF
AAAKLDWSKRTSSEMLEFYRMLLDLRHQRIVPLLKGAGGGNAVSRSAGSTLVVDWALAGDRRLHLDANLGSEAAPLLQQR
NEGEMIFRLGGSDGGKLAPWTVIWSISKGEGMKAIGR

Sequences:

>Translated_597_residues
MTKMIFGPAFTEDGVLFRLWAPLHESVSLKIEGTDPRPMQAAEDGWHQCTVADAGPGTQYRFILPDGLQIPDPASRFQPQ
DVHGASEVVDLAAYRWNTREWTGRPWEEMVIYELHIGCFTPQGTFKAAIDRLDHLRELGVTALQIMPLSDFPGRYNWGYD
GVLPYAPDSTYGRPEDLMALVDAAHERSISVFLDAVYNHFGPDGNYIPSYAPLFTDHHRTPWGHGINYDGDGSEMIREFF
IENATYWINEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEANDSDLLKRDADGEARLFTAQWND
DVHHVLHITATGETFGYYADYAGDAGKLGRALTEGFVFQGEHMPYRGRSRGRPSGHLPPTAFVSFIQNHDQIGNRALGDR
IMASSPTDAVKAVTSIYLLAPQIPMLFMGEEWGAREPFPFFCDFDESLNEKVRKGRREELSRLPGFDSDELLDPTAPSTF
AAAKLDWSKRTSSEMLEFYRMLLDLRHQRIVPLLKGAGGGNAVSRSAGSTLVVDWALAGDRRLHLDANLGSEAAPLLQQR
NEGEMIFRLGGSDGGKLAPWTVIWSISKGEGMKAIGR
>Mature_596_residues
TKMIFGPAFTEDGVLFRLWAPLHESVSLKIEGTDPRPMQAAEDGWHQCTVADAGPGTQYRFILPDGLQIPDPASRFQPQD
VHGASEVVDLAAYRWNTREWTGRPWEEMVIYELHIGCFTPQGTFKAAIDRLDHLRELGVTALQIMPLSDFPGRYNWGYDG
VLPYAPDSTYGRPEDLMALVDAAHERSISVFLDAVYNHFGPDGNYIPSYAPLFTDHHRTPWGHGINYDGDGSEMIREFFI
ENATYWINEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEANDSDLLKRDADGEARLFTAQWNDD
VHHVLHITATGETFGYYADYAGDAGKLGRALTEGFVFQGEHMPYRGRSRGRPSGHLPPTAFVSFIQNHDQIGNRALGDRI
MASSPTDAVKAVTSIYLLAPQIPMLFMGEEWGAREPFPFFCDFDESLNEKVRKGRREELSRLPGFDSDELLDPTAPSTFA
AAKLDWSKRTSSEMLEFYRMLLDLRHQRIVPLLKGAGGGNAVSRSAGSTLVVDWALAGDRRLHLDANLGSEAAPLLQQRN
EGEMIFRLGGSDGGKLAPWTVIWSISKGEGMKAIGR

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=166, Percent_Identity=29.5180722891566, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1789839, Length=281, Percent_Identity=26.3345195729537, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367229, Length=405, Percent_Identity=24.1975308641975, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554896, Length=176, Percent_Identity=30.6818181818182, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32564391, Length=176, Percent_Identity=30.6818181818182, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320826, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28573410, Length=212, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 66702; Mature: 66570

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMIFGPAFTEDGVLFRLWAPLHESVSLKIEGTDPRPMQAAEDGWHQCTVADAGPGTQY
CCCEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHCCCCCEEEEECCCCCCEE
RFILPDGLQIPDPASRFQPQDVHGASEVVDLAAYRWNTREWTGRPWEEMVIYELHIGCFT
EEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEEEEEC
PQGTFKAAIDRLDHLRELGVTALQIMPLSDFPGRYNWGYDGVLPYAPDSTYGRPEDLMAL
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VDAAHERSISVFLDAVYNHFGPDGNYIPSYAPLFTDHHRTPWGHGINYDGDGSEMIREFF
HHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IENATYWINEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEANDS
HCCCCEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
DLLKRDADGEARLFTAQWNDDVHHVLHITATGETFGYYADYAGDAGKLGRALTEGFVFQG
HHHHCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCEEEC
EHMPYRGRSRGRPSGHLPPTAFVSFIQNHDQIGNRALGDRIMASSPTDAVKAVTSIYLLA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHCCCCHHHHHHHHHHHHHC
PQIPMLFMGEEWGAREPFPFFCDFDESLNEKVRKGRREELSRLPGFDSDELLDPTAPSTF
CCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCC
AAAKLDWSKRTSSEMLEFYRMLLDLRHQRIVPLLKGAGGGNAVSRSAGSTLVVDWALAGD
EEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCC
RRLHLDANLGSEAAPLLQQRNEGEMIFRLGGSDGGKLAPWTVIWSISKGEGMKAIGR
EEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHCCC
>Mature Secondary Structure 
TKMIFGPAFTEDGVLFRLWAPLHESVSLKIEGTDPRPMQAAEDGWHQCTVADAGPGTQY
CCEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHCCCCCEEEEECCCCCCEE
RFILPDGLQIPDPASRFQPQDVHGASEVVDLAAYRWNTREWTGRPWEEMVIYELHIGCFT
EEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEEEEEC
PQGTFKAAIDRLDHLRELGVTALQIMPLSDFPGRYNWGYDGVLPYAPDSTYGRPEDLMAL
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VDAAHERSISVFLDAVYNHFGPDGNYIPSYAPLFTDHHRTPWGHGINYDGDGSEMIREFF
HHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IENATYWINEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEANDS
HCCCCEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
DLLKRDADGEARLFTAQWNDDVHHVLHITATGETFGYYADYAGDAGKLGRALTEGFVFQG
HHHHCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCEEEC
EHMPYRGRSRGRPSGHLPPTAFVSFIQNHDQIGNRALGDRIMASSPTDAVKAVTSIYLLA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHCCCCHHHHHHHHHHHHHC
PQIPMLFMGEEWGAREPFPFFCDFDESLNEKVRKGRREELSRLPGFDSDELLDPTAPSTF
CCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCC
AAAKLDWSKRTSSEMLEFYRMLLDLRHQRIVPLLKGAGGGNAVSRSAGSTLVVDWALAGD
EEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCC
RRLHLDANLGSEAAPLLQQRNEGEMIFRLGGSDGGKLAPWTVIWSISKGEGMKAIGR
EEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]