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The map label for this gene is dhaK [H]

Identifier: 86360765

GI number: 86360765

Start: 31234

End: 32190

Strand: Reverse

Name: dhaK [H]

Synonym: RHE_PF00031

Alternate gene names: 86360765

Gene position: 32190-31234 (Counterclockwise)

Preceding gene: 86360766

Following gene: 86360764

Centisome position: 5.01

GC content: 64.58

Gene sequence:

>957_bases
ATGCTGCAAGGGGTTCTCGCGGCACATCCCGAGCACCTCTATGCCGTGGACGACGTGCCGCGCGCCATCATTGCCAGGAA
CGGACCGCGCAAGGGCAAGGTCGGGCTGGTGATCGGCGGCGGCTCCGGGCATGAGCCGACATTCCTCGGCTATGTCGGCA
AGGGGCTCGCCGATGCCGCCGCGATCGGCAATGTCTTCGCCTCGCCGCCGCCCGATCCGATCATGGCCTGCGCCAAGGCC
GTCGATGGCGGCGTCGGGGTGCTCTTCATGTACGGCAATTATGCCGGCGACGTGATGAATTTCGACATGGCGGCGGACAT
GCTGGCGCTCGACGACATCACGGTGCGTACCGTGCTGACCACCGACGATGTCGCGTCCGCCCCGGCCGATCAGACGGACA
GGCGGCGCGGCGTGGCGGGCAATGTCTTCATCTTCAAGGCCGCCGGTGCTGCCTGCGATCTTCTCTATTCCTTCAATGAG
GTCGAGCGCGCGGCCCGCCATGCCAATGCGCGCACCTATACGATGGGTGTTGCGCTCTCGCCCTGCTCGCTGCCCCAGAC
CTTGAAACCGAACTTCCAGATCGGCGAGGAGGAGATGGAGATCGGCATGGGCATCCACGGCGAGCCCGGCGTGGCGCGCG
GGCCGCTGAAGACGGCCGATGCCGTGACCGACGAACTGATGGACAATCTTCTCAGGGAGATGAAGGCCGAGCGTGGCGAT
CGTGTCGCCGTGCTTGTTAATTCGCTGGGCTCGACGCCGATGATGGAACTCTACATCATGATGCGGCGCGTCAAGGCGCG
GCTCGACGAGGCCGGGCTCGTCATCCACACATCGCTCGTCGGCAATTACTGCACCTCGCTCGAAATGGCGGGCGCGTCCA
TCACGCTGATGCATCTCGACGACGAGCTTCAGCGGCTGATCGATCATCCCTGCGATTGCGCCATGTTCAGGAGGTGA

Upstream 100 bases:

>100_bases
TTGACCTCTGGCCCGCCCGTCGCAGTGGGAGCTCGTTCTGGGGAGGATAGGCGTGAAGACGAAGAAGCTAATCAATGCAG
GCGCGGATGCCGTCGATGAG

Downstream 100 bases:

>100_bases
TCGATGACCATCGCAACCGAGGACCTGCAGCGCCTGTTTGCCCTGATTGCTAGGGATATCGCCGCGCGACGCGATCATCT
TTGCGAGCTAGACGGCGCCA

Product: dihydroxyacetone kinase domain-containing protein

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MLQGVLAAHPEHLYAVDDVPRAIIARNGPRKGKVGLVIGGGSGHEPTFLGYVGKGLADAAAIGNVFASPPPDPIMACAKA
VDGGVGVLFMYGNYAGDVMNFDMAADMLALDDITVRTVLTTDDVASAPADQTDRRRGVAGNVFIFKAAGAACDLLYSFNE
VERAARHANARTYTMGVALSPCSLPQTLKPNFQIGEEEMEIGMGIHGEPGVARGPLKTADAVTDELMDNLLREMKAERGD
RVAVLVNSLGSTPMMELYIMMRRVKARLDEAGLVIHTSLVGNYCTSLEMAGASITLMHLDDELQRLIDHPCDCAMFRR

Sequences:

>Translated_318_residues
MLQGVLAAHPEHLYAVDDVPRAIIARNGPRKGKVGLVIGGGSGHEPTFLGYVGKGLADAAAIGNVFASPPPDPIMACAKA
VDGGVGVLFMYGNYAGDVMNFDMAADMLALDDITVRTVLTTDDVASAPADQTDRRRGVAGNVFIFKAAGAACDLLYSFNE
VERAARHANARTYTMGVALSPCSLPQTLKPNFQIGEEEMEIGMGIHGEPGVARGPLKTADAVTDELMDNLLREMKAERGD
RVAVLVNSLGSTPMMELYIMMRRVKARLDEAGLVIHTSLVGNYCTSLEMAGASITLMHLDDELQRLIDHPCDCAMFRR
>Mature_318_residues
MLQGVLAAHPEHLYAVDDVPRAIIARNGPRKGKVGLVIGGGSGHEPTFLGYVGKGLADAAAIGNVFASPPPDPIMACAKA
VDGGVGVLFMYGNYAGDVMNFDMAADMLALDDITVRTVLTTDDVASAPADQTDRRRGVAGNVFIFKAAGAACDLLYSFNE
VERAARHANARTYTMGVALSPCSLPQTLKPNFQIGEEEMEIGMGIHGEPGVARGPLKTADAVTDELMDNLLREMKAERGD
RVAVLVNSLGSTPMMELYIMMRRVKARLDEAGLVIHTSLVGNYCTSLEMAGASITLMHLDDELQRLIDHPCDCAMFRR

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=293, Percent_Identity=36.8600682593857, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI87081857, Length=334, Percent_Identity=41.3173652694611, Blast_Score=251, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17565018, Length=278, Percent_Identity=37.410071942446, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321055, Length=312, Percent_Identity=33.0128205128205, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323570, Length=312, Percent_Identity=31.4102564102564, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 33981; Mature: 33981

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQGVLAAHPEHLYAVDDVPRAIIARNGPRKGKVGLVIGGGSGHEPTFLGYVGKGLADAA
CCCCCCCCCCCCEEEHHHCCHHHHHCCCCCCCCEEEEEECCCCCCCEEEHHHCCCHHHHH
AIGNVFASPPPDPIMACAKAVDGGVGVLFMYGNYAGDVMNFDMAADMLALDDITVRTVLT
HHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHCCEEEEEEEE
TDDVASAPADQTDRRRGVAGNVFIFKAAGAACDLLYSFNEVERAARHANARTYTMGVALS
CCCCCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEC
PCSLPQTLKPNFQIGEEEMEIGMGIHGEPGVARGPLKTADAVTDELMDNLLREMKAERGD
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RVAVLVNSLGSTPMMELYIMMRRVKARLDEAGLVIHTSLVGNYCTSLEMAGASITLMHLD
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEECCCEEEEEECC
DELQRLIDHPCDCAMFRR
HHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MLQGVLAAHPEHLYAVDDVPRAIIARNGPRKGKVGLVIGGGSGHEPTFLGYVGKGLADAA
CCCCCCCCCCCCEEEHHHCCHHHHHCCCCCCCCEEEEEECCCCCCCEEEHHHCCCHHHHH
AIGNVFASPPPDPIMACAKAVDGGVGVLFMYGNYAGDVMNFDMAADMLALDDITVRTVLT
HHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHCCEEEEEEEE
TDDVASAPADQTDRRRGVAGNVFIFKAAGAACDLLYSFNEVERAARHANARTYTMGVALS
CCCCCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEC
PCSLPQTLKPNFQIGEEEMEIGMGIHGEPGVARGPLKTADAVTDELMDNLLREMKAERGD
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RVAVLVNSLGSTPMMELYIMMRRVKARLDEAGLVIHTSLVGNYCTSLEMAGASITLMHLD
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEECCCEEEEEECC
DELQRLIDHPCDCAMFRR
HHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]