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The map label for this gene is tilS [H]

Identifier: 85859371

GI number: 85859371

Start: 1569819

End: 1571249

Strand: Direct

Name: tilS [H]

Synonym: SYN_02776

Alternate gene names: 85859371

Gene position: 1569819-1571249 (Clockwise)

Preceding gene: 85859370

Following gene: 85859372

Centisome position: 49.38

GC content: 48.29

Gene sequence:

>1431_bases
ATGCTGGATAAAATCAGACGTACCATTCAAAAATACCATATGCTGAATCCCGGTGAGCATGTGATTGCGGCTGTTTCGGG
AGGGCCGGATTCCGTCGCGCTCCTTCATGTTCTGATCAGCCTTGCCTGCGAATATGAATTGACTCTGTCTGTAGCTCATT
TGAATCATGGTCTGCGGGGCTCCGAATCCGATGCAGAAGAGCAGCTGGTGAGACAGTTGAGCAAGGAAAGAGGGATTTCC
TGCTCCGTCAAAAGGATCGATCTCCAGGCGCTCAGAAAATGCGATAGACGAAAGTGCTCTCTGGAGGATATAGGACGGCA
GGAAAGATACGAATTTTTTACGGCCCTGGCTGCCGAGATCGGCGCTTCACGGATCGCCCTTGGACACCATCGGGAAGATC
AGGCGGAAACGGTTCTGATGAATTTCATCCGCGGGAGCGGTCTGGAGGGACTCAAAGGAATCCGGCCTGTGCGTGACGGA
TGCTTCATTCGTCCCCTGCTTGAAGTGAGCAGAAGGGATATTCTCGACTTTCTTCAGGCCGAGCATCTTCCTTTTCTTGA
GGACAGTTCGAACAGCGAAGATCACTTTCTGCGAAACAGGATACGTCACCATATGATTCCTCTTCTGCAGGAACTGTATA
ACCCGAGAATCATCGACACACTGAATCGGTTGGCTGAAATCGCCCGCAGAGAAGATGACTGTTTGAAGTCCATCGTTGAT
CGCATTCTTGAGGATTTGCTGATTACCTCACTTTCTGAAGAAGGGACAGTAAGTATTTCCATCTCAAGACTTCACGAGCT
TGATGAGGCTCTTCAATACCGTCTGGTCAAATCCCTGCTGGAACGCTGCGTTTTGTCGCCTCGCCGGGTTGCCTTTACCC
ACATTCAGGCCGTTGCGAACCTCTGTAGGGAGGAGAAATCCGTCGGTCTTCTGTTTCTCCCCGGAAATGTCCTTGTGAGA
CGTGAATACGATAACCTGAAATTTTCCTTATCGGAGGCGAACACACGATCCGAATTGAATTCTCCACTGAAAAACCAAAA
GGTAATAGGTTTTTCCTATGCGTTAGAAATTCCATCTGTCACGGAAATTGAAGAAGTGAACAGAGTTGTAAAAACAGCGT
TTATAGAAAAAGTTGAGCTTGGGTTCTTTAATGTAACTCGGAATTCGGCCTGTTTTGACTTTGACAAGATTGAACCGCCC
CTTGTAATCCGGAATCGTCGAGAAGGGGATCGTTTCCAACCCCTGGGCATGGCGGGGACAAAAAAGCTGAAGTCATACTT
TATCGATGAAAAAGTACCAAGGCGTCAGCGGGATCTTATTCCTCTCCTGGCAGATCGACATTCCGTGGTCTGGATTGCGG
GGCTGAGGCTCAGTGAAAGGGTACGGATTGACGATAGGACAAGATCAATTGTAAAAATAGAAATGATTTGA

Upstream 100 bases:

>100_bases
GGGGAGCAGGGAGGAGCTTTTATCTTCAAAACCAGATTATGTCTGCGAAACCCTTCCTGAATTGACGAGACTCTTCTGCT
GAAATGCGGAAAAGAGATCC

Downstream 100 bases:

>100_bases
AATAAAATCAGGATTATTGTAATTGTGCGTTGGTAGTTGATTTATTGATTTAATATATTATTATACTGACATTAAGTAAT
AATTATTATAAGGAAAAATA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MLDKIRRTIQKYHMLNPGEHVIAAVSGGPDSVALLHVLISLACEYELTLSVAHLNHGLRGSESDAEEQLVRQLSKERGIS
CSVKRIDLQALRKCDRRKCSLEDIGRQERYEFFTALAAEIGASRIALGHHREDQAETVLMNFIRGSGLEGLKGIRPVRDG
CFIRPLLEVSRRDILDFLQAEHLPFLEDSSNSEDHFLRNRIRHHMIPLLQELYNPRIIDTLNRLAEIARREDDCLKSIVD
RILEDLLITSLSEEGTVSISISRLHELDEALQYRLVKSLLERCVLSPRRVAFTHIQAVANLCREEKSVGLLFLPGNVLVR
REYDNLKFSLSEANTRSELNSPLKNQKVIGFSYALEIPSVTEIEEVNRVVKTAFIEKVELGFFNVTRNSACFDFDKIEPP
LVIRNRREGDRFQPLGMAGTKKLKSYFIDEKVPRRQRDLIPLLADRHSVVWIAGLRLSERVRIDDRTRSIVKIEMI

Sequences:

>Translated_476_residues
MLDKIRRTIQKYHMLNPGEHVIAAVSGGPDSVALLHVLISLACEYELTLSVAHLNHGLRGSESDAEEQLVRQLSKERGIS
CSVKRIDLQALRKCDRRKCSLEDIGRQERYEFFTALAAEIGASRIALGHHREDQAETVLMNFIRGSGLEGLKGIRPVRDG
CFIRPLLEVSRRDILDFLQAEHLPFLEDSSNSEDHFLRNRIRHHMIPLLQELYNPRIIDTLNRLAEIARREDDCLKSIVD
RILEDLLITSLSEEGTVSISISRLHELDEALQYRLVKSLLERCVLSPRRVAFTHIQAVANLCREEKSVGLLFLPGNVLVR
REYDNLKFSLSEANTRSELNSPLKNQKVIGFSYALEIPSVTEIEEVNRVVKTAFIEKVELGFFNVTRNSACFDFDKIEPP
LVIRNRREGDRFQPLGMAGTKKLKSYFIDEKVPRRQRDLIPLLADRHSVVWIAGLRLSERVRIDDRTRSIVKIEMI
>Mature_476_residues
MLDKIRRTIQKYHMLNPGEHVIAAVSGGPDSVALLHVLISLACEYELTLSVAHLNHGLRGSESDAEEQLVRQLSKERGIS
CSVKRIDLQALRKCDRRKCSLEDIGRQERYEFFTALAAEIGASRIALGHHREDQAETVLMNFIRGSGLEGLKGIRPVRDG
CFIRPLLEVSRRDILDFLQAEHLPFLEDSSNSEDHFLRNRIRHHMIPLLQELYNPRIIDTLNRLAEIARREDDCLKSIVD
RILEDLLITSLSEEGTVSISISRLHELDEALQYRLVKSLLERCVLSPRRVAFTHIQAVANLCREEKSVGLLFLPGNVLVR
REYDNLKFSLSEANTRSELNSPLKNQKVIGFSYALEIPSVTEIEEVNRVVKTAFIEKVELGFFNVTRNSACFDFDKIEPP
LVIRNRREGDRFQPLGMAGTKKLKSYFIDEKVPRRQRDLIPLLADRHSVVWIAGLRLSERVRIDDRTRSIVKIEMI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=453, Percent_Identity=26.7108167770419, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787606, Length=188, Percent_Identity=26.063829787234, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54541; Mature: 54541

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKIRRTIQKYHMLNPGEHVIAAVSGGPDSVALLHVLISLACEYELTLSVAHLNHGLRG
CHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEEHHCCCCCC
SESDAEEQLVRQLSKERGISCSVKRIDLQALRKCDRRKCSLEDIGRQERYEFFTALAAEI
CCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHH
GASRIALGHHREDQAETVLMNFIRGSGLEGLKGIRPVRDGCFIRPLLEVSRRDILDFLQA
CHHHEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHH
EHLPFLEDSSNSEDHFLRNRIRHHMIPLLQELYNPRIIDTLNRLAEIARREDDCLKSIVD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHH
RILEDLLITSLSEEGTVSISISRLHELDEALQYRLVKSLLERCVLSPRRVAFTHIQAVAN
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LCREEKSVGLLFLPGNVLVRREYDNLKFSLSEANTRSELNSPLKNQKVIGFSYALEIPSV
HHHHHHCCEEEEECCCEEEEEECCCCEEEECCCCCHHHHCCCCCCCEEEEEEEEECCCCC
TEIEEVNRVVKTAFIEKVELGFFNVTRNSACFDFDKIEPPLVIRNRREGDRFQPLGMAGT
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHH
KKLKSYFIDEKVPRRQRDLIPLLADRHSVVWIAGLRLSERVRIDDRTRSIVKIEMI
HHHHHHHHCCCCCCHHHHCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MLDKIRRTIQKYHMLNPGEHVIAAVSGGPDSVALLHVLISLACEYELTLSVAHLNHGLRG
CHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEEHHCCCCCC
SESDAEEQLVRQLSKERGISCSVKRIDLQALRKCDRRKCSLEDIGRQERYEFFTALAAEI
CCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHH
GASRIALGHHREDQAETVLMNFIRGSGLEGLKGIRPVRDGCFIRPLLEVSRRDILDFLQA
CHHHEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHH
EHLPFLEDSSNSEDHFLRNRIRHHMIPLLQELYNPRIIDTLNRLAEIARREDDCLKSIVD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHH
RILEDLLITSLSEEGTVSISISRLHELDEALQYRLVKSLLERCVLSPRRVAFTHIQAVAN
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LCREEKSVGLLFLPGNVLVRREYDNLKFSLSEANTRSELNSPLKNQKVIGFSYALEIPSV
HHHHHHCCEEEEECCCEEEEEECCCCEEEECCCCCHHHHCCCCCCCEEEEEEEEECCCCC
TEIEEVNRVVKTAFIEKVELGFFNVTRNSACFDFDKIEPPLVIRNRREGDRFQPLGMAGT
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHH
KKLKSYFIDEKVPRRQRDLIPLLADRHSVVWIAGLRLSERVRIDDRTRSIVKIEMI
HHHHHHHHCCCCCCHHHHCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]