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The map label for this gene is pcrA [H]

Identifier: 85859318

GI number: 85859318

Start: 1507588

End: 1509561

Strand: Direct

Name: pcrA [H]

Synonym: SYN_02537

Alternate gene names: 85859318

Gene position: 1507588-1509561 (Clockwise)

Preceding gene: 85859317

Following gene: 85859323

Centisome position: 47.42

GC content: 54.0

Gene sequence:

>1974_bases
ATGATCGATTATGAGAAGGAACTGAACGAAGAGCAGTGTCACGTTGTGCTGGAAGCGGGAGGGCCCATGCTGGTTCTTGC
CGGCGCGGGGAGCGGAAAAACCCGGACGCTGACCTATCGGGTGGCGCGCCTGGTTGAGACCGGGATCAAACCCGAGCGGA
TTTTGCTGGCCACGTTCACTAACAAGGCTGCCCGTTCCATGCTCAACCGGGTCCGGGAACTCGTTCCTGTGGATCTCAGC
CGCCTCTCCGGCGGAACCTTTCATCACAACGGTCATTTCATGTTGCGGGCTCACGCCGAAAGGCTGGGCTATACACGGAG
CTTTTCCATACTCGATACGGAAGACGCCCGGCAGTTGATCTCGACCTGCGTTGTCGAGGCGGGCATCGATACGAAAGGGG
CGACCTTTCCCAAGGCCAATGTTCTTTATGGGATATTGAGCCTTGCCGTGAATACAGCGGCCGATCTTTCCACGACCGTA
GCGGAACGCTATCCTTTTTTCAGCCATCGTCTTGAGGATATCCGAAAAGTCGCCGCTCTTTATGATCTGCGCAAGCGTCA
GTCCGATTTCATGGATTTTGACGACCTGCTCCTCAACTGGCGTCGCCTGCTGCTGGAATGTCCCGATGTCCTGCAACATT
ACGCTGAACGCTTTGAACACATTCTCGTGGATGAATATCAGGACACGAATCGCATCCAGGCGGAAATCATGGATCTGCTG
GCATCACGACACCGCAACATCATGGTCGTAGGGGATGACTCCCAGAGCATTTACGCCTTCCGCGGGGCCAACTTTGCCAA
CATCATGTCTTTCCCGGAAAAATATCCGGACTGCCGGATTTTCAAACTGGAGACCAATTACCGCAGCACCCCGGAAATTC
TCCATCTGGCAAATCTCAGCATTGAAAACAATGAAAATCAGTTTGCCAAGTCTCTGCGGGCCGTCCGGGAAGCGGGTCTG
AGACCGGTGGTCGTCCCTGCCCGGAATGCCCTGCAACAGGCCGAATTTGTTGCCCAGCGGATACTGGAGCTTCACAGAGA
CGGCATTCCTCTGCACGAAATGGCGATTCTGTACCGGGCCCATTTTCACGCCATGGATGTTCAGATGGAGTTGACACGGC
GGGGAATCCCCTATGAAATCCGTTCGGGAATCCGCTTTTTCGAACAGGCCCACATCAAGGATGTAACCGCTTATCTGAGA
ATTCTGGGGAATCCGCTGGATGAACTGGCATGGAAACGGATTTTTGGGCTGTGTCCGAAGATCGGGAAAAAAACTGCCGA
GAAACTCTGGCAATTTCTTTCGGAATCGGGAGAACCGCTGGAAGCTGCTTTAAGGGAAGATTTTCTGAAGGTGGTTTCGA
AGACGGCCAGAGGCGGACTCGCTTTTTTTCAGGAAGTATTGATCAAGCTGCGGGAGGAGGCTGTTCATGCTTCGACGCTG
CCGGATCTCATCAGCCTGATTATTGAGAGAGGATATCGAAAGTATCTGCGGGAAACGTATACCGATACGGCCTCACGGGA
AGAAGATCTCGAACAGCTCGCGGAATTTTCCGGCAAGTTTTCCTCTCTGGAGGACTTTTTGAGTGAATTGTCCCTCATGA
CCAACATGGACGCCGACGGACGGGACAAGGCAGCCGGCGATGACCTCCAGGAAAACAAGGTCATTTTGAGCACCGTTCAT
CAGGCAAAGGGGTTGGAATGGTCCGTTGTCCTGCTCGTCGGATGCGCTGAAGGCATGATTCCCCTGGAACGGGCGGTTCA
GGAAGCCGGCGGCGAAGAAGAAGAGAGGAGACTTTTTTATGTGGCCGCCACCCGGGCCAAGGATTACCTCTACCTCTGCT
ATCCCCAGACGGATTATTCAAGATGGGCCGGGTTTGCCAATCTCAGCCCATCCCGGTTCATTCGAGAACTGGTTCCCTCC
TCCCGAAAAAAAGAACTGCCCTTCGAACACTGGGTTGTCGAAGAGCAGTGGTGA

Upstream 100 bases:

>100_bases
GCTGCTCAAGACATTTTCCTCAGTCAGCAGCGCCGAAACCGTGGAAGCCGTTAAAAAGGCCTTGAAGGAATGCTGATCAA
AATACCGGGTCTGTTTCGCC

Downstream 100 bases:

>100_bases
GTTCCTGTATTTATCTGTTTACAGCCAGTGATCTGATTTGTATTTCGCGTCATATTCCGCTTTCAGGGCGATCTGCGTTG
CTTCGGCGATATATTCAGGC

Product: uvrD/rep helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MIDYEKELNEEQCHVVLEAGGPMLVLAGAGSGKTRTLTYRVARLVETGIKPERILLATFTNKAARSMLNRVRELVPVDLS
RLSGGTFHHNGHFMLRAHAERLGYTRSFSILDTEDARQLISTCVVEAGIDTKGATFPKANVLYGILSLAVNTAADLSTTV
AERYPFFSHRLEDIRKVAALYDLRKRQSDFMDFDDLLLNWRRLLLECPDVLQHYAERFEHILVDEYQDTNRIQAEIMDLL
ASRHRNIMVVGDDSQSIYAFRGANFANIMSFPEKYPDCRIFKLETNYRSTPEILHLANLSIENNENQFAKSLRAVREAGL
RPVVVPARNALQQAEFVAQRILELHRDGIPLHEMAILYRAHFHAMDVQMELTRRGIPYEIRSGIRFFEQAHIKDVTAYLR
ILGNPLDELAWKRIFGLCPKIGKKTAEKLWQFLSESGEPLEAALREDFLKVVSKTARGGLAFFQEVLIKLREEAVHASTL
PDLISLIIERGYRKYLRETYTDTASREEDLEQLAEFSGKFSSLEDFLSELSLMTNMDADGRDKAAGDDLQENKVILSTVH
QAKGLEWSVVLLVGCAEGMIPLERAVQEAGGEEEERRLFYVAATRAKDYLYLCYPQTDYSRWAGFANLSPSRFIRELVPS
SRKKELPFEHWVVEEQW

Sequences:

>Translated_657_residues
MIDYEKELNEEQCHVVLEAGGPMLVLAGAGSGKTRTLTYRVARLVETGIKPERILLATFTNKAARSMLNRVRELVPVDLS
RLSGGTFHHNGHFMLRAHAERLGYTRSFSILDTEDARQLISTCVVEAGIDTKGATFPKANVLYGILSLAVNTAADLSTTV
AERYPFFSHRLEDIRKVAALYDLRKRQSDFMDFDDLLLNWRRLLLECPDVLQHYAERFEHILVDEYQDTNRIQAEIMDLL
ASRHRNIMVVGDDSQSIYAFRGANFANIMSFPEKYPDCRIFKLETNYRSTPEILHLANLSIENNENQFAKSLRAVREAGL
RPVVVPARNALQQAEFVAQRILELHRDGIPLHEMAILYRAHFHAMDVQMELTRRGIPYEIRSGIRFFEQAHIKDVTAYLR
ILGNPLDELAWKRIFGLCPKIGKKTAEKLWQFLSESGEPLEAALREDFLKVVSKTARGGLAFFQEVLIKLREEAVHASTL
PDLISLIIERGYRKYLRETYTDTASREEDLEQLAEFSGKFSSLEDFLSELSLMTNMDADGRDKAAGDDLQENKVILSTVH
QAKGLEWSVVLLVGCAEGMIPLERAVQEAGGEEEERRLFYVAATRAKDYLYLCYPQTDYSRWAGFANLSPSRFIRELVPS
SRKKELPFEHWVVEEQW
>Mature_657_residues
MIDYEKELNEEQCHVVLEAGGPMLVLAGAGSGKTRTLTYRVARLVETGIKPERILLATFTNKAARSMLNRVRELVPVDLS
RLSGGTFHHNGHFMLRAHAERLGYTRSFSILDTEDARQLISTCVVEAGIDTKGATFPKANVLYGILSLAVNTAADLSTTV
AERYPFFSHRLEDIRKVAALYDLRKRQSDFMDFDDLLLNWRRLLLECPDVLQHYAERFEHILVDEYQDTNRIQAEIMDLL
ASRHRNIMVVGDDSQSIYAFRGANFANIMSFPEKYPDCRIFKLETNYRSTPEILHLANLSIENNENQFAKSLRAVREAGL
RPVVVPARNALQQAEFVAQRILELHRDGIPLHEMAILYRAHFHAMDVQMELTRRGIPYEIRSGIRFFEQAHIKDVTAYLR
ILGNPLDELAWKRIFGLCPKIGKKTAEKLWQFLSESGEPLEAALREDFLKVVSKTARGGLAFFQEVLIKLREEAVHASTL
PDLISLIIERGYRKYLRETYTDTASREEDLEQLAEFSGKFSSLEDFLSELSLMTNMDADGRDKAAGDDLQENKVILSTVH
QAKGLEWSVVLLVGCAEGMIPLERAVQEAGGEEEERRLFYVAATRAKDYLYLCYPQTDYSRWAGFANLSPSRFIRELVPS
SRKKELPFEHWVVEEQW

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=635, Percent_Identity=35.5905511811024, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI48994965, Length=651, Percent_Identity=32.258064516129, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322369, Length=708, Percent_Identity=26.1299435028249, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324477, Length=686, Percent_Identity=22.3032069970845, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75092; Mature: 75092

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYEKELNEEQCHVVLEAGGPMLVLAGAGSGKTRTLTYRVARLVETGIKPERILLATFT
CCCCHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NKAARSMLNRVRELVPVDLSRLSGGTFHHNGHFMLRAHAERLGYTRSFSILDTEDARQLI
CHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCEEEEEHHHHCCCCCEEEEECCHHHHHHH
STCVVEAGIDTKGATFPKANVLYGILSLAVNTAADLSTTVAERYPFFSHRLEDIRKVAAL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YDLRKRQSDFMDFDDLLLNWRRLLLECPDVLQHYAERFEHILVDEYQDTNRIQAEIMDLL
HHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ASRHRNIMVVGDDSQSIYAFRGANFANIMSFPEKYPDCRIFKLETNYRSTPEILHLANLS
HHCCCCEEEEECCCCCEEEECCCCHHHHHHCHHHCCCCEEEEEECCCCCCCHHEEHCCCC
IENNENQFAKSLRAVREAGLRPVVVPARNALQQAEFVAQRILELHRDGIPLHEMAILYRA
CCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
HFHAMDVQMELTRRGIPYEIRSGIRFFEQAHIKDVTAYLRILGNPLDELAWKRIFGLCPK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHH
IGKKTAEKLWQFLSESGEPLEAALREDFLKVVSKTARGGLAFFQEVLIKLREEAVHASTL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
PDLISLIIERGYRKYLRETYTDTASREEDLEQLAEFSGKFSSLEDFLSELSLMTNMDADG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
RDKAAGDDLQENKVILSTVHQAKGLEWSVVLLVGCAEGMIPLERAVQEAGGEEEERRLFY
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCHHCEEEE
VAATRAKDYLYLCYPQTDYSRWAGFANLSPSRFIRELVPSSRKKELPFEHWVVEEQW
EEEECCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MIDYEKELNEEQCHVVLEAGGPMLVLAGAGSGKTRTLTYRVARLVETGIKPERILLATFT
CCCCHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NKAARSMLNRVRELVPVDLSRLSGGTFHHNGHFMLRAHAERLGYTRSFSILDTEDARQLI
CHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCEEEEEHHHHCCCCCEEEEECCHHHHHHH
STCVVEAGIDTKGATFPKANVLYGILSLAVNTAADLSTTVAERYPFFSHRLEDIRKVAAL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YDLRKRQSDFMDFDDLLLNWRRLLLECPDVLQHYAERFEHILVDEYQDTNRIQAEIMDLL
HHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ASRHRNIMVVGDDSQSIYAFRGANFANIMSFPEKYPDCRIFKLETNYRSTPEILHLANLS
HHCCCCEEEEECCCCCEEEECCCCHHHHHHCHHHCCCCEEEEEECCCCCCCHHEEHCCCC
IENNENQFAKSLRAVREAGLRPVVVPARNALQQAEFVAQRILELHRDGIPLHEMAILYRA
CCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
HFHAMDVQMELTRRGIPYEIRSGIRFFEQAHIKDVTAYLRILGNPLDELAWKRIFGLCPK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHH
IGKKTAEKLWQFLSESGEPLEAALREDFLKVVSKTARGGLAFFQEVLIKLREEAVHASTL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
PDLISLIIERGYRKYLRETYTDTASREEDLEQLAEFSGKFSSLEDFLSELSLMTNMDADG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
RDKAAGDDLQENKVILSTVHQAKGLEWSVVLLVGCAEGMIPLERAVQEAGGEEEERRLFY
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCHHCEEEE
VAATRAKDYLYLCYPQTDYSRWAGFANLSPSRFIRELVPSSRKKELPFEHWVVEEQW
EEEECCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA