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The map label for this gene is miaB

Identifier: 85859266

GI number: 85859266

Start: 1452986

End: 1454371

Strand: Direct

Name: miaB

Synonym: SYN_02149

Alternate gene names: 85859266

Gene position: 1452986-1454371 (Clockwise)

Preceding gene: 85859263

Following gene: 85859267

Centisome position: 45.7

GC content: 44.23

Gene sequence:

>1386_bases
ATGATGAAAAGCAGTAATAAATCTTCAGGATCATTGATTAGATTGGATAAAAAGCATATTTACATACAGACACTCGGCTG
TCAGATGAATGTTCACGATTCTGAACAGATTGCGGCACTGATGGAGGAAAAAGGGTATATCTGTACGGAGGATGCAAACG
AGGCGGATCTGATTATACTCAACACATGCAGCATTCGGGAAAAGGCGGCTCAGAAGGCAAAAAGTCAACTGGGGCGGTAC
CGAAACCTGAAGAGAAAAAAAAGAAATCTGCTCATCGGTGTGGGGGGGTGTCTCGCTCAACAACTGGGGGATGAGCTTCT
TACAAAAGTGCCGGATATCGATTTTATTTTCGGTACACACAATATTCACCAGCTTCCGGATTTCATTTCCAGGATAGAAA
AAAGCAGAAAAAAAATTGTGGAAACGACTCTTCATCCCTCTACGCCGTCCATCGGTGTGTTGGCTTTGCCTTGTAATGGT
CAGGTTTCAAGTTTTGTGACGATCATGCAGGGATGCAATAATTTTTGTTCGTATTGCATTGTTCCCTACGTCCGCGGCCG
TGAAGAAAGTCGACCCCCGGAAGATATTATCCATGAAATCAGAATGCTGGCCGACCACGGTGTTAAGGAAGTCACGCTTC
TCGGACAGAATGTCAACTCCTATGCGAGAAAGACAAGTGGAGAGATGGGCTTTGCGGAACTGCTTCGGGAAATTGAAAAG
ATCAAAGGAATTGAGCGGATGCGGTTTACCACGTCTCATCCTAAGGATCTTTCCGAATTCCTCATTACTGCGTTTTCAGA
TTTGAGCAAGTTGTGCCACCACATTCACCTGCCTTTTCAATCAGGATCAGATCGGATTCTTGCCTTGATGAACCGTGGAT
ATACGAAATCGGACTATCTGGCAAAAGTTGAACGTTTGAGAACAGTCTGCCCAGATATCAGTATTACTGCAGATGTCATT
GTCGGATTTCCAGGCGAGTCGGACGAAGATTTCAAAGAAACAATTGACATGATGAATCAAATCAGATTTGATAATCTTTT
TTCATTTAAATATTCGGAACGGGAAGGAACCGCAGCCGTAAAAATGGACGGAAAAGTGAGCGAACCGCTCAAGCTTGAAA
GGCTGCAGATTTTACAGGCACTTCAGGAACAGCATACATTGGAGAAAAACAAGGCGATGGAAGGAAAACAGGAAGACGTC
CTGGTGGAAGGATTCAGTAAAAATTGCCGAAAAGACCTTACTGGACGAACATCGACGAATAAAATCGTCAACTTCAGCGG
GTGTGTTGATCTTATTGGAGACATGGTGTCCGTGCTCATTAAAGAAGCCTATTTGCATTCCCTCAGGGGAGAAATGCTCT
GCGAGGAGGTCGTTCATGCAAATTGA

Upstream 100 bases:

>100_bases
TCCCGCCGGAAAAGCTGTTTTACAACATAATTCAGCATCTCCAATCCTTAAAATTCCTTTGTAAATAAATATTTGTTGAC
TAAGTTCATGTAGCATATTA

Downstream 100 bases:

>100_bases
AATGAAAGTGTCCGGACTGACGATTGATCCGATTACCAATACGCCGATTGTAATTCTGAAGGATTTTCAAGAGAAAAAGG
TCATACCCATCTGGATCGGC

Product: tRNA 2-methylthioadenosine synthase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MMKSSNKSSGSLIRLDKKHIYIQTLGCQMNVHDSEQIAALMEEKGYICTEDANEADLIILNTCSIREKAAQKAKSQLGRY
RNLKRKKRNLLIGVGGCLAQQLGDELLTKVPDIDFIFGTHNIHQLPDFISRIEKSRKKIVETTLHPSTPSIGVLALPCNG
QVSSFVTIMQGCNNFCSYCIVPYVRGREESRPPEDIIHEIRMLADHGVKEVTLLGQNVNSYARKTSGEMGFAELLREIEK
IKGIERMRFTTSHPKDLSEFLITAFSDLSKLCHHIHLPFQSGSDRILALMNRGYTKSDYLAKVERLRTVCPDISITADVI
VGFPGESDEDFKETIDMMNQIRFDNLFSFKYSEREGTAAVKMDGKVSEPLKLERLQILQALQEQHTLEKNKAMEGKQEDV
LVEGFSKNCRKDLTGRTSTNKIVNFSGCVDLIGDMVSVLIKEAYLHSLRGEMLCEEVVHAN

Sequences:

>Translated_461_residues
MMKSSNKSSGSLIRLDKKHIYIQTLGCQMNVHDSEQIAALMEEKGYICTEDANEADLIILNTCSIREKAAQKAKSQLGRY
RNLKRKKRNLLIGVGGCLAQQLGDELLTKVPDIDFIFGTHNIHQLPDFISRIEKSRKKIVETTLHPSTPSIGVLALPCNG
QVSSFVTIMQGCNNFCSYCIVPYVRGREESRPPEDIIHEIRMLADHGVKEVTLLGQNVNSYARKTSGEMGFAELLREIEK
IKGIERMRFTTSHPKDLSEFLITAFSDLSKLCHHIHLPFQSGSDRILALMNRGYTKSDYLAKVERLRTVCPDISITADVI
VGFPGESDEDFKETIDMMNQIRFDNLFSFKYSEREGTAAVKMDGKVSEPLKLERLQILQALQEQHTLEKNKAMEGKQEDV
LVEGFSKNCRKDLTGRTSTNKIVNFSGCVDLIGDMVSVLIKEAYLHSLRGEMLCEEVVHAN
>Mature_461_residues
MMKSSNKSSGSLIRLDKKHIYIQTLGCQMNVHDSEQIAALMEEKGYICTEDANEADLIILNTCSIREKAAQKAKSQLGRY
RNLKRKKRNLLIGVGGCLAQQLGDELLTKVPDIDFIFGTHNIHQLPDFISRIEKSRKKIVETTLHPSTPSIGVLALPCNG
QVSSFVTIMQGCNNFCSYCIVPYVRGREESRPPEDIIHEIRMLADHGVKEVTLLGQNVNSYARKTSGEMGFAELLREIEK
IKGIERMRFTTSHPKDLSEFLITAFSDLSKLCHHIHLPFQSGSDRILALMNRGYTKSDYLAKVERLRTVCPDISITADVI
VGFPGESDEDFKETIDMMNQIRFDNLFSFKYSEREGTAAVKMDGKVSEPLKLERLQILQALQEQHTLEKNKAMEGKQEDV
LVEGFSKNCRKDLTGRTSTNKIVNFSGCVDLIGDMVSVLIKEAYLHSLRGEMLCEEVVHAN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=35.0730688935282, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=35.0730688935282, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI93277076, Length=462, Percent_Identity=26.1904761904762, Blast_Score=158, Evalue=8e-39,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=45.0226244343891, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=27.6404494382022, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=32.8326180257511, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=276, Percent_Identity=28.6231884057971, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=33.4728033472803, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19922432, Length=453, Percent_Identity=27.1523178807947, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_SYNAS (Q2LT94)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461468.1
- ProteinModelPortal:   Q2LT94
- SMR:   Q2LT94
- STRING:   Q2LT94
- GeneID:   3884299
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02149
- NMPDR:   fig|56780.10.peg.1406
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   Q2LT94
- BioCyc:   SACI56780:SYN_02149-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51939; Mature: 51939

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKSSNKSSGSLIRLDKKHIYIQTLGCQMNVHDSEQIAALMEEKGYICTEDANEADLIIL
CCCCCCCCCCCEEEEECCEEEEEEECCEECCCCHHHHHHHHHHCCCEEECCCCCCCEEEE
NTCSIREKAAQKAKSQLGRYRNLKRKKRNLLIGVGGCLAQQLGDELLTKVPDIDFIFGTH
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHCCCCEEEECCC
NIHQLPDFISRIEKSRKKIVETTLHPSTPSIGVLALPCNGQVSSFVTIMQGCNNFCSYCI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VPYVRGREESRPPEDIIHEIRMLADHGVKEVTLLGQNVNSYARKTSGEMGFAELLREIEK
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHHH
IKGIERMRFTTSHPKDLSEFLITAFSDLSKLCHHIHLPFQSGSDRILALMNRGYTKSDYL
HCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHH
AKVERLRTVCPDISITADVIVGFPGESDEDFKETIDMMNQIRFDNLFSFKYSEREGTAAV
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEE
KMDGKVSEPLKLERLQILQALQEQHTLEKNKAMEGKQEDVLVEGFSKNCRKDLTGRTSTN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
KIVNFSGCVDLIGDMVSVLIKEAYLHSLRGEMLCEEVVHAN
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKSSNKSSGSLIRLDKKHIYIQTLGCQMNVHDSEQIAALMEEKGYICTEDANEADLIIL
CCCCCCCCCCCEEEEECCEEEEEEECCEECCCCHHHHHHHHHHCCCEEECCCCCCCEEEE
NTCSIREKAAQKAKSQLGRYRNLKRKKRNLLIGVGGCLAQQLGDELLTKVPDIDFIFGTH
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHCCCCEEEECCC
NIHQLPDFISRIEKSRKKIVETTLHPSTPSIGVLALPCNGQVSSFVTIMQGCNNFCSYCI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VPYVRGREESRPPEDIIHEIRMLADHGVKEVTLLGQNVNSYARKTSGEMGFAELLREIEK
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHHH
IKGIERMRFTTSHPKDLSEFLITAFSDLSKLCHHIHLPFQSGSDRILALMNRGYTKSDYL
HCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHH
AKVERLRTVCPDISITADVIVGFPGESDEDFKETIDMMNQIRFDNLFSFKYSEREGTAAV
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEE
KMDGKVSEPLKLERLQILQALQEQHTLEKNKAMEGKQEDVLVEGFSKNCRKDLTGRTSTN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
KIVNFSGCVDLIGDMVSVLIKEAYLHSLRGEMLCEEVVHAN
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA