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The map label for this gene is gatB

Identifier: 85858295

GI number: 85858295

Start: 462933

End: 464369

Strand: Direct

Name: gatB

Synonym: SYN_02604

Alternate gene names: 85858295

Gene position: 462933-464369 (Clockwise)

Preceding gene: 85858294

Following gene: 85858296

Centisome position: 14.56

GC content: 60.96

Gene sequence:

>1437_bases
ATGGAATTTGAAGCGGTTATCGGATTGGAGATTCACATCGAACTCAATTGTCCGACCAAGTTGTTCTGCGATTGTCCCAA
CAACCCCGGCGACGAACCCAACGTCAACACCTGCCCGATCTGCCTGTGGTTTCCCGGAGCCATTCCCCGCCTCAGCCAGG
CGGCCCTGGAAAAGGCGTCGCTGCTGTGCCTGGGACTGGGCGCCGAACTTCAGCCCCGCAGCGCCTTCGACCAGAAGGTT
TACTACTACCCGGATCTGCCCAAGGGCTACCAGCTGTCCCAGGCCCATCTGCCCCTGGCCCGGGGAGGCGGGATCGACAT
TACGGATGAAAACGGCCGGCCGAAGCGGCTGCGCATCCACCATATCCACATGGAAGAAGACGTTGCCAAACTGGTCCATG
AAATCGAGGGACGAACCCCCATCAGCCTCGTGGATTTCAACCGCGCCGGAGCGCCCCTCGTGGAAATCGTCAGCGAACCC
GATTTTCGCACTCCCCACGACGCCATGGAGTTTCTCAAGGCCCTCCGCACGCAGGTGCGCTACGTGGGCGCTTCCGAATG
CAGTATGGAAAACGGCACCATGCGCGTTGATGCCAACATCTCCGTGCGTCCCCGGGGAACGGACCAGATGAATACCAAGG
TGGAAGTCAAGAACATGAATTCCATCCGGCATGTGGGAGATGCCGTTGCCTATGAGATCAGCCGCCAGAGCGCCTGCGTC
TCGTCGGGGGAGGCCGTGGTTCTCCATACCCGCCTCTGGGATCCTGACAGGAAGGCGACGTTCCCCATGCGGGCCAAGTT
TGAAGGGCCGTGCGTCCCCGATCCGTCGGTGCCTTTCATCGATCTGTCTCCGGAATGGATAGAAAAGATGCGGGCGAGAC
TTCCCGAAATGCCTGCCGCGCGGGCCGAACGCTTCGTCGCCCGGTACGGCCTGACCGACGAAGAGGCAGTCTATCTGAGC
GCCGATCCGGAAACGGCCTCTTATTTCGAAGCCCTCATCGCCGAGAAGGTCGCGCCGCGCACTGCCATGCACTGGCTCAC
CACCCAGCTGCTTGCCGCCGTCAGGGAGCGTGGGCAGGAGTTGAGCGGCACGCCGGTAACTCCCGCGCGCTTTGCCGCCC
TGCTGAAAATGCTGGCCAAAGACGAAATCAACGCCAATGCCGCCCGGCAGGTCCTGATCGAGCTGTTTGATTGCGGGGAG
AGTCCGGAGAAGATTGTCGAAGCCCGGGGGATTCGGCAGGTATCCGATCACGACGCCCTTGAGGGCCTTATTGACAGGGT
GCTGGGTGAAAATCCGGCGGCGGTTGCCGATTACCGGGGCGGCCAGGGAAAGGCCGCGGGGTTTCTCATCGGCAAGGTGA
TGCAGGCCTCCGGAGGCAAGGCCAATCCGAAGATTATCAGGGAATTGCTTACAAAAAAACTGGACGCCCTGGGATAA

Upstream 100 bases:

>100_bases
TTACACCGGGCCTGCAGCTTGCGGGACCGAGGCTCGGCGACGCCCGCCTTCTCGCCTTGGGCGAATATCTTCTGAGTGCA
CGCCAGGGAGGTAAATAGTC

Downstream 100 bases:

>100_bases
CCGGGAGCCCTGACAACAGAAATATCAGCCGACAGTCTTTGCAGGGGAAAATGATGAATATGAAAGACTGCGGCTGATTT
GCCTGCAACCCCGCCCGATC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B 1

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEFEAVIGLEIHIELNCPTKLFCDCPNNPGDEPNVNTCPICLWFPGAIPRLSQAALEKASLLCLGLGAELQPRSAFDQKV
YYYPDLPKGYQLSQAHLPLARGGGIDITDENGRPKRLRIHHIHMEEDVAKLVHEIEGRTPISLVDFNRAGAPLVEIVSEP
DFRTPHDAMEFLKALRTQVRYVGASECSMENGTMRVDANISVRPRGTDQMNTKVEVKNMNSIRHVGDAVAYEISRQSACV
SSGEAVVLHTRLWDPDRKATFPMRAKFEGPCVPDPSVPFIDLSPEWIEKMRARLPEMPAARAERFVARYGLTDEEAVYLS
ADPETASYFEALIAEKVAPRTAMHWLTTQLLAAVRERGQELSGTPVTPARFAALLKMLAKDEINANAARQVLIELFDCGE
SPEKIVEARGIRQVSDHDALEGLIDRVLGENPAAVADYRGGQGKAAGFLIGKVMQASGGKANPKIIRELLTKKLDALG

Sequences:

>Translated_478_residues
MEFEAVIGLEIHIELNCPTKLFCDCPNNPGDEPNVNTCPICLWFPGAIPRLSQAALEKASLLCLGLGAELQPRSAFDQKV
YYYPDLPKGYQLSQAHLPLARGGGIDITDENGRPKRLRIHHIHMEEDVAKLVHEIEGRTPISLVDFNRAGAPLVEIVSEP
DFRTPHDAMEFLKALRTQVRYVGASECSMENGTMRVDANISVRPRGTDQMNTKVEVKNMNSIRHVGDAVAYEISRQSACV
SSGEAVVLHTRLWDPDRKATFPMRAKFEGPCVPDPSVPFIDLSPEWIEKMRARLPEMPAARAERFVARYGLTDEEAVYLS
ADPETASYFEALIAEKVAPRTAMHWLTTQLLAAVRERGQELSGTPVTPARFAALLKMLAKDEINANAARQVLIELFDCGE
SPEKIVEARGIRQVSDHDALEGLIDRVLGENPAAVADYRGGQGKAAGFLIGKVMQASGGKANPKIIRELLTKKLDALG
>Mature_478_residues
MEFEAVIGLEIHIELNCPTKLFCDCPNNPGDEPNVNTCPICLWFPGAIPRLSQAALEKASLLCLGLGAELQPRSAFDQKV
YYYPDLPKGYQLSQAHLPLARGGGIDITDENGRPKRLRIHHIHMEEDVAKLVHEIEGRTPISLVDFNRAGAPLVEIVSEP
DFRTPHDAMEFLKALRTQVRYVGASECSMENGTMRVDANISVRPRGTDQMNTKVEVKNMNSIRHVGDAVAYEISRQSACV
SSGEAVVLHTRLWDPDRKATFPMRAKFEGPCVPDPSVPFIDLSPEWIEKMRARLPEMPAARAERFVARYGLTDEEAVYLS
ADPETASYFEALIAEKVAPRTAMHWLTTQLLAAVRERGQELSGTPVTPARFAALLKMLAKDEINANAARQVLIELFDCGE
SPEKIVEARGIRQVSDHDALEGLIDRVLGENPAAVADYRGGQGKAAGFLIGKVMQASGGKANPKIIRELLTKKLDALG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=33, Blast_Score=246, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI115534094, Length=388, Percent_Identity=31.701030927835, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319391, Length=526, Percent_Identity=28.8973384030418, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=29.2089249492901, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB1_SYNAS (Q2LPY6)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460497.1
- ProteinModelPortal:   Q2LPY6
- SMR:   Q2LPY6
- STRING:   Q2LPY6
- GeneID:   3885264
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02604
- NMPDR:   fig|56780.10.peg.450
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   YQITQNE
- PhylomeDB:   Q2LPY6
- ProtClustDB:   PRK05477
- BioCyc:   SACI56780:SYN_02604-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52653; Mature: 52653

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFEAVIGLEIHIELNCPTKLFCDCPNNPGDEPNVNTCPICLWFPGAIPRLSQAALEKAS
CCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
LLCLGLGAELQPRSAFDQKVYYYPDLPKGYQLSQAHLPLARGGGIDITDENGRPKRLRIH
EEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEECCCCEEEECCCCCCCEEEEE
HIHMEEDVAKLVHEIEGRTPISLVDFNRAGAPLVEIVSEPDFRTPHDAMEFLKALRTQVR
EECCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
YVGASECSMENGTMRVDANISVRPRGTDQMNTKVEVKNMNSIRHVGDAVAYEISRQSACV
HCCCCCCCCCCCCEEEECCEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
SSGEAVVLHTRLWDPDRKATFPMRAKFEGPCVPDPSVPFIDLSPEWIEKMRARLPEMPAA
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RAERFVARYGLTDEEAVYLSADPETASYFEALIAEKVAPRTAMHWLTTQLLAAVRERGQE
HHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
LSGTPVTPARFAALLKMLAKDEINANAARQVLIELFDCGESPEKIVEARGIRQVSDHDAL
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
EGLIDRVLGENPAAVADYRGGQGKAAGFLIGKVMQASGGKANPKIIRELLTKKLDALG
HHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFEAVIGLEIHIELNCPTKLFCDCPNNPGDEPNVNTCPICLWFPGAIPRLSQAALEKAS
CCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
LLCLGLGAELQPRSAFDQKVYYYPDLPKGYQLSQAHLPLARGGGIDITDENGRPKRLRIH
EEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEECCCCEEEECCCCCCCEEEEE
HIHMEEDVAKLVHEIEGRTPISLVDFNRAGAPLVEIVSEPDFRTPHDAMEFLKALRTQVR
EECCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
YVGASECSMENGTMRVDANISVRPRGTDQMNTKVEVKNMNSIRHVGDAVAYEISRQSACV
HCCCCCCCCCCCCEEEECCEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
SSGEAVVLHTRLWDPDRKATFPMRAKFEGPCVPDPSVPFIDLSPEWIEKMRARLPEMPAA
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RAERFVARYGLTDEEAVYLSADPETASYFEALIAEKVAPRTAMHWLTTQLLAAVRERGQE
HHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
LSGTPVTPARFAALLKMLAKDEINANAARQVLIELFDCGESPEKIVEARGIRQVSDHDAL
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
EGLIDRVLGENPAAVADYRGGQGKAAGFLIGKVMQASGGKANPKIIRELLTKKLDALG
HHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA