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The map label for this gene is gatA [H]

Identifier: 85858294

GI number: 85858294

Start: 461569

End: 462930

Strand: Direct

Name: gatA [H]

Synonym: SYN_02603

Alternate gene names: 85858294

Gene position: 461569-462930 (Clockwise)

Preceding gene: 85858293

Following gene: 85858295

Centisome position: 14.52

GC content: 61.97

Gene sequence:

>1362_bases
GTGTGGCGCCCGTCGCCAACCGCGTCCGGGAGGGCCTCGAGGCCGTGCGGTTGTCGTTTTCCGGTACCGGCCGGCTGCTC
AAGAACGCCCCGGCGGTCAAGGGAGATTACTTCAAAGTCGCCGGCATTCTGGATTAAACACACAACAGAAGGAGAAGCCT
TCATGGAAGACATTTTTTGCTATACCAGTCCTCAAACTGCCTCCTCCGGGAGTGGCCCCCTGCGGGGACTCAAAACGGCG
ATTCAGCCCTGTTTCTGTGTGGCGGAATGGCCGACCGATGCCGGTTCCAGGGCTCTGGCCGGTTTTACAGCCCTGGAGGA
CGCGACCGTCGTGGAGCGCCTGCGCCAGGCCGGCGCCGTTCTGACCGGTCTGACCCGGATGAGCGAATTCGGGTTCGGCC
TCGATGACAGCCGGGCCGGCATGGCCATCCGGAATCAGGCGGCCGATGTCGAACTGTCGCTGGACCTGATGGGCGAGACC
CGCCTGGCCGCGGCGCGGGCTTCGGTCTGCGGTTTCAAACCCAGTTGGGGGATCGTTTCCCGTTTCGGACTCATCGGCCT
GATGCCGTCCATGGAATGCTGCGGCCTCCTTTCCGGCGGTGTGAAGCAGATCCGGGAGGTCCTGAAAGTCATCGCCGGCC
CCGATGAGCGGGACTTTTCCCTTCCCGATGAGAAAACGCCGGATTTTTCCCAGGGTGAAATAAACCCCCGGGAAACAACC
ATCGGTGTCGTTCCGGAAGGGCTGAGCAACATGACTTCCGAACAGAGGGACGCGTTCGAGGCCGCCCTTGCGGAGTTGAG
GGAAGCGGGCTTTTCCCTCAGGGAGATTTCCCTGCCCGATTTTTCTCTTTTTGCGCTGGTTCATCGGATCGTCGGCTCCG
TGGAAGCGTCGTCCAGCGCCGGGCGTTACGATTCGGTGCGCTACGGTCAGCGGGCAAAGGGGGCAAAAAACTGGAATGAG
ATGTATCTCCTTTCCCGCGGCGCCGCCTTCGGGACCCTTGTGAAAAGCTATCTGTTTCAAGGGGCTTTTTTCCAGTTTGA
ACGCTACGACGCCTATGAAGACGCCTGCCGCGTCCGGGCCCGCCTCCTTGCGGCCATGGCGGAAATCAGCGCCCAGGCGG
ACTTTCTGGCCCTGCCTGCGGTCAATCCCGGGGTTCCTGACATGGCTGCATCGTTGGATGATACGTACCGGCAATTCTTC
TATACGGCCTTCGCCAACGTCACCGGCCAGCCGACTCTGTATCTGCCGCCCGGCGCGGGTGCGATTACACCGGGCCTGCA
GCTTGCGGGACCGAGGCTCGGCGACGCCCGCCTTCTCGCCTTGGGCGAATATCTTCTGAGTGCACGCCAGGGAGGTAAAT
AG

Upstream 100 bases:

>100_bases
GCCGAAGGAGAGCGTCCCGTGATGGAAAGCATTCTCGCCCAGGCCGAGGAACTGGCTGCCCAGGTTGGGGATCTGGCGGG
GAACGAAGAGCCGGTGCGGA

Downstream 100 bases:

>100_bases
TCATGGAATTTGAAGCGGTTATCGGATTGGAGATTCACATCGAACTCAATTGTCCGACCAAGTTGTTCTGCGATTGTCCC
AACAACCCCGGCGACGAACC

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MWRPSPTASGRASRPCGCRFPVPAGCSRTPRRSREITSKSPAFWIKHTTEGEAFMEDIFCYTSPQTASSGSGPLRGLKTA
IQPCFCVAEWPTDAGSRALAGFTALEDATVVERLRQAGAVLTGLTRMSEFGFGLDDSRAGMAIRNQAADVELSLDLMGET
RLAAARASVCGFKPSWGIVSRFGLIGLMPSMECCGLLSGGVKQIREVLKVIAGPDERDFSLPDEKTPDFSQGEINPRETT
IGVVPEGLSNMTSEQRDAFEAALAELREAGFSLREISLPDFSLFALVHRIVGSVEASSSAGRYDSVRYGQRAKGAKNWNE
MYLLSRGAAFGTLVKSYLFQGAFFQFERYDAYEDACRVRARLLAAMAEISAQADFLALPAVNPGVPDMAASLDDTYRQFF
YTAFANVTGQPTLYLPPGAGAITPGLQLAGPRLGDARLLALGEYLLSARQGGK

Sequences:

>Translated_453_residues
MWRPSPTASGRASRPCGCRFPVPAGCSRTPRRSREITSKSPAFWIKHTTEGEAFMEDIFCYTSPQTASSGSGPLRGLKTA
IQPCFCVAEWPTDAGSRALAGFTALEDATVVERLRQAGAVLTGLTRMSEFGFGLDDSRAGMAIRNQAADVELSLDLMGET
RLAAARASVCGFKPSWGIVSRFGLIGLMPSMECCGLLSGGVKQIREVLKVIAGPDERDFSLPDEKTPDFSQGEINPRETT
IGVVPEGLSNMTSEQRDAFEAALAELREAGFSLREISLPDFSLFALVHRIVGSVEASSSAGRYDSVRYGQRAKGAKNWNE
MYLLSRGAAFGTLVKSYLFQGAFFQFERYDAYEDACRVRARLLAAMAEISAQADFLALPAVNPGVPDMAASLDDTYRQFF
YTAFANVTGQPTLYLPPGAGAITPGLQLAGPRLGDARLLALGEYLLSARQGGK
>Mature_453_residues
MWRPSPTASGRASRPCGCRFPVPAGCSRTPRRSREITSKSPAFWIKHTTEGEAFMEDIFCYTSPQTASSGSGPLRGLKTA
IQPCFCVAEWPTDAGSRALAGFTALEDATVVERLRQAGAVLTGLTRMSEFGFGLDDSRAGMAIRNQAADVELSLDLMGET
RLAAARASVCGFKPSWGIVSRFGLIGLMPSMECCGLLSGGVKQIREVLKVIAGPDERDFSLPDEKTPDFSQGEINPRETT
IGVVPEGLSNMTSEQRDAFEAALAELREAGFSLREISLPDFSLFALVHRIVGSVEASSSAGRYDSVRYGQRAKGAKNWNE
MYLLSRGAAFGTLVKSYLFQGAFFQFERYDAYEDACRVRARLLAAMAEISAQADFLALPAVNPGVPDMAASLDDTYRQFF
YTAFANVTGQPTLYLPPGAGAITPGLQLAGPRLGDARLLALGEYLLSARQGGK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=429, Percent_Identity=26.1072261072261, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=23.8425925925926, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323950, Length=436, Percent_Identity=25.2293577981651, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648113, Length=420, Percent_Identity=30.4761904761905, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48804; Mature: 48804

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRPSPTASGRASRPCGCRFPVPAGCSRTPRRSREITSKSPAFWIKHTTEGEAFMEDIFC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCHHHHHHHHH
YTSPQTASSGSGPLRGLKTAIQPCFCVAEWPTDAGSRALAGFTALEDATVVERLRQAGAV
CCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTGLTRMSEFGFGLDDSRAGMAIRNQAADVELSLDLMGETRLAAARASVCGFKPSWGIVS
HHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCHHHH
RFGLIGLMPSMECCGLLSGGVKQIREVLKVIAGPDERDFSLPDEKTPDFSQGEINPRETT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IGVVPEGLSNMTSEQRDAFEAALAELREAGFSLREISLPDFSLFALVHRIVGSVEASSSA
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCC
GRYDSVRYGQRAKGAKNWNEMYLLSRGAAFGTLVKSYLFQGAFFQFERYDAYEDACRVRA
CCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLAAMAEISAQADFLALPAVNPGVPDMAASLDDTYRQFFYTAFANVTGQPTLYLPPGAG
HHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
AITPGLQLAGPRLGDARLLALGEYLLSARQGGK
CCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MWRPSPTASGRASRPCGCRFPVPAGCSRTPRRSREITSKSPAFWIKHTTEGEAFMEDIFC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCHHHHHHHHH
YTSPQTASSGSGPLRGLKTAIQPCFCVAEWPTDAGSRALAGFTALEDATVVERLRQAGAV
CCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTGLTRMSEFGFGLDDSRAGMAIRNQAADVELSLDLMGETRLAAARASVCGFKPSWGIVS
HHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCHHHH
RFGLIGLMPSMECCGLLSGGVKQIREVLKVIAGPDERDFSLPDEKTPDFSQGEINPRETT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IGVVPEGLSNMTSEQRDAFEAALAELREAGFSLREISLPDFSLFALVHRIVGSVEASSSA
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCC
GRYDSVRYGQRAKGAKNWNEMYLLSRGAAFGTLVKSYLFQGAFFQFERYDAYEDACRVRA
CCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLAAMAEISAQADFLALPAVNPGVPDMAASLDDTYRQFFYTAFANVTGQPTLYLPPGAG
HHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
AITPGLQLAGPRLGDARLLALGEYLLSARQGGK
CCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA