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The map label for this gene is yfnG [H]

Identifier: 85858242

GI number: 85858242

Start: 397517

End: 398512

Strand: Direct

Name: yfnG [H]

Synonym: SYN_01130

Alternate gene names: 85858242

Gene position: 397517-398512 (Clockwise)

Preceding gene: 85858241

Following gene: 85858243

Centisome position: 12.5

GC content: 61.75

Gene sequence:

>996_bases
ATGGGAAGTTTCTGGGCGGATAAGAAAGTGCTGGTAACCGGCGCCGGTGGATTTATCGGCAGTCACCTGGTGGAGCGGCT
TGCCGGGGAAGGGGCTTCAGTGCGGGCCTTCGTCCGCTACAACTCCCGTGCCGACGCGGGGCTGCTCAAGCTGGCGCCTC
CGGAGATTCTTTCCCGCATCGAGCTGATCGGCGGAGATCTGCGGGATTCCGATGCCGTGCGCAAGGCCGTGGAAGGCTGC
CGGATTGTGTTCCACCTGGGTGCGCTCATTTCCATTCCCTATTCCTATTATCATCCCGTGGAAGTGGCCGAGACGAATCT
CATGGGGACGTTGAACGTGCTTCTCGCCTGCCGCGACCTCGGCGTGGAGCGCCTGATCCACACGTCCACCAGCGAAGTCT
ATGGCACCGCCCAGCGCGTCCCCATCGACGAGGCGCATCCCCTCCAGGGGCAGTCCCCCTATTCCGCCAGCAAGATCGGG
GCGGACAAACTGGCGGAGAGCTTCTTCTGCGCCTTCAGCGTGCCGGTGGTGACCGTCCGTCCCTTCAACACATTCGGCCC
GCGCCAGTCCGCCCGCGCAGTCATCCCCACGATCATCACCCAGGTGCTTGCCCGGCAGAGCATCCGTCTGGGAAATCTCG
AAACCACCCGTGATTTCACCTACGTAGACGACACCGTCAGCGGTTTTCTCTGTGCCGCGCAGAAGGAGGGCATCGAAGGC
ATGACCTTCAACCTGGGGACGGGGTCGGAGATTGCGATCGGCGTTCTTGCCCGGACAATCATCGAAAAAATCGGAGTCCC
CGTTTCAGTCGAACTGGATCCGGAGCGGCTCCGTCCGGAAGGCTCCGAGGTGATGCGGCTTCTGTCCGACAATTCGCGGG
CCCGTTCCCAACTCGGGTGGGGGCCCGAAGTGGGCCTGGATGAAGGACTCAACAGGACCATCGCCTGGATTCGGGAGAAT
CTGGGGAATTACCGCATCGGCGTTTATGAATTATAG

Upstream 100 bases:

>100_bases
CCACGTGGAGACGCCGGCCGACGGGAAGTAACGCTTCCTTGATCTATGCAGAGGTCTGCAGTTCTGCGGGAATTTCATCA
CAACAGGAGGGAAAGATCAC

Downstream 100 bases:

>100_bases
TATGAATTATAGAAGGGAGGGCAACCTATGAAAGCCGTAGTCCTGGCAGGCGGAAAAGGCGCACGCCTCGCGCCTTACAC
AAAAATTCTTCCCAAACCGT

Product: dTDP-glucose 4,6-dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MGSFWADKKVLVTGAGGFIGSHLVERLAGEGASVRAFVRYNSRADAGLLKLAPPEILSRIELIGGDLRDSDAVRKAVEGC
RIVFHLGALISIPYSYYHPVEVAETNLMGTLNVLLACRDLGVERLIHTSTSEVYGTAQRVPIDEAHPLQGQSPYSASKIG
ADKLAESFFCAFSVPVVTVRPFNTFGPRQSARAVIPTIITQVLARQSIRLGNLETTRDFTYVDDTVSGFLCAAQKEGIEG
MTFNLGTGSEIAIGVLARTIIEKIGVPVSVELDPERLRPEGSEVMRLLSDNSRARSQLGWGPEVGLDEGLNRTIAWIREN
LGNYRIGVYEL

Sequences:

>Translated_331_residues
MGSFWADKKVLVTGAGGFIGSHLVERLAGEGASVRAFVRYNSRADAGLLKLAPPEILSRIELIGGDLRDSDAVRKAVEGC
RIVFHLGALISIPYSYYHPVEVAETNLMGTLNVLLACRDLGVERLIHTSTSEVYGTAQRVPIDEAHPLQGQSPYSASKIG
ADKLAESFFCAFSVPVVTVRPFNTFGPRQSARAVIPTIITQVLARQSIRLGNLETTRDFTYVDDTVSGFLCAAQKEGIEG
MTFNLGTGSEIAIGVLARTIIEKIGVPVSVELDPERLRPEGSEVMRLLSDNSRARSQLGWGPEVGLDEGLNRTIAWIREN
LGNYRIGVYEL
>Mature_330_residues
GSFWADKKVLVTGAGGFIGSHLVERLAGEGASVRAFVRYNSRADAGLLKLAPPEILSRIELIGGDLRDSDAVRKAVEGCR
IVFHLGALISIPYSYYHPVEVAETNLMGTLNVLLACRDLGVERLIHTSTSEVYGTAQRVPIDEAHPLQGQSPYSASKIGA
DKLAESFFCAFSVPVVTVRPFNTFGPRQSARAVIPTIITQVLARQSIRLGNLETTRDFTYVDDTVSGFLCAAQKEGIEGM
TFNLGTGSEIAIGVLARTIIEKIGVPVSVELDPERLRPEGSEVMRLLSDNSRARSQLGWGPEVGLDEGLNRTIAWIRENL
GNYRIGVYEL

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=31.9018404907975, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=30.1829268292683, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4504031, Length=331, Percent_Identity=29.0030211480363, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=348, Percent_Identity=27.2988505747126, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56118217, Length=348, Percent_Identity=27.2988505747126, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI189083684, Length=348, Percent_Identity=27.2988505747126, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI193211614, Length=354, Percent_Identity=23.4463276836158, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI8393516, Length=354, Percent_Identity=23.4463276836158, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI48994969, Length=342, Percent_Identity=28.6549707602339, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=28.7749287749288, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=26.7605633802817, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788365, Length=346, Percent_Identity=27.1676300578035, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786974, Length=343, Percent_Identity=26.8221574344023, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=339, Percent_Identity=29.4985250737463, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI115532424, Length=335, Percent_Identity=25.6716417910448, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=27.9279279279279, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI133901786, Length=348, Percent_Identity=26.1494252873563, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17539422, Length=348, Percent_Identity=26.1494252873563, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI133901788, Length=348, Percent_Identity=26.1494252873563, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI133901790, Length=348, Percent_Identity=26.1494252873563, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17539424, Length=348, Percent_Identity=26.1494252873563, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17507723, Length=349, Percent_Identity=25.7879656160458, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=351, Percent_Identity=25.9259259259259, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71987463, Length=196, Percent_Identity=34.6938775510204, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321437, Length=360, Percent_Identity=26.1111111111111, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=357, Percent_Identity=24.9299719887955, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=31.9148936170213, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24158427, Length=333, Percent_Identity=27.6276276276276, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19923002, Length=350, Percent_Identity=27.1428571428571, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 35962; Mature: 35830

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFWADKKVLVTGAGGFIGSHLVERLAGEGASVRAFVRYNSRADAGLLKLAPPEILSRI
CCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCHHHHHHH
ELIGGDLRDSDAVRKAVEGCRIVFHLGALISIPYSYYHPVEVAETNLMGTLNVLLACRDL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
GVERLIHTSTSEVYGTAQRVPIDEAHPLQGQSPYSASKIGADKLAESFFCAFSVPVVTVR
CHHHHHHCCCHHHHCCHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEE
PFNTFGPRQSARAVIPTIITQVLARQSIRLGNLETTRDFTYVDDTVSGFLCAAQKEGIEG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHEEHHHHCCCCC
MTFNLGTGSEIAIGVLARTIIEKIGVPVSVELDPERLRPEGSEVMRLLSDNSRARSQLGW
EEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHCCHHHHHHCCC
GPEVGLDEGLNRTIAWIRENLGNYRIGVYEL
CCCCCHHHCHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
GSFWADKKVLVTGAGGFIGSHLVERLAGEGASVRAFVRYNSRADAGLLKLAPPEILSRI
CCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCHHHHHHH
ELIGGDLRDSDAVRKAVEGCRIVFHLGALISIPYSYYHPVEVAETNLMGTLNVLLACRDL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
GVERLIHTSTSEVYGTAQRVPIDEAHPLQGQSPYSASKIGADKLAESFFCAFSVPVVTVR
CHHHHHHCCCHHHHCCHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEE
PFNTFGPRQSARAVIPTIITQVLARQSIRLGNLETTRDFTYVDDTVSGFLCAAQKEGIEG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHEEHHHHCCCCC
MTFNLGTGSEIAIGVLARTIIEKIGVPVSVELDPERLRPEGSEVMRLLSDNSRARSQLGW
EEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHCCHHHHHHCCC
GPEVGLDEGLNRTIAWIRENLGNYRIGVYEL
CCCCCHHHCHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]