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The map label for this gene is folC [H]

Identifier: 85858177

GI number: 85858177

Start: 329970

End: 331367

Strand: Direct

Name: folC [H]

Synonym: SYN_01606

Alternate gene names: 85858177

Gene position: 329970-331367 (Clockwise)

Preceding gene: 85858176

Following gene: 85858178

Centisome position: 10.38

GC content: 48.43

Gene sequence:

>1398_bases
TTGGACGGGATCGATTACGGAATTTCAGGGAGTCGGCCTGAAGGTTCTGCGAAGAATATCGTTTGTGAAGTAATTATAAA
TTGTACAATGGAAAATGTTCAAAAAGAAGATCCACAACGTTTATTAGATTTAAACTGCAATATCATTCGTCTCGGTCTTG
ACCCAATCAGGCATCTTCTTGAAAGAATGGAGAATCCCCAACTCAAGTATCCCGCGGTTCTAATTGGAGGAACCAACGGC
AAGGGATCCACAGCGGCCATTCTATCTTGCATCCTCAAGGAAAGCGGCTATAAAGTCGGACTTTATACTTCACCACATTT
GACGAGCATTTGCGAACGAATCCGTATTAATGGTTCCTTAATATCTGAGGAACGAATGCAAGACTGCTTCGGGATGATCC
AAAGCAGTCTTGAGGAGCCGCTGACCTATTTTGAGGTTCTGACAGCGTCGGCATATCTTTATTTTTATCAGGAGAAGGTT
GATATTGCGGTTCTGGAAGTGGGCATGGGGGGACGTCTTGATGCGACCAACGTTGTAACGCCCCTGGTCTCCGTCATCTC
GAACGTTCATCTGGAACACCAGGAATATCTGGGACGTCATTTAAGAGACATTGCTGCCGAAAAAGCGGGAATTATCAAAG
AGAATAGCGTCTGTGTTACCGCTGTTACCCAGAAAGCCGTTCTTGACCTTCTGTCAGAAACATGTCGACAGAAAGAAGCG
ACCCTGTATCGCCTCGGGAAAGATATCCGCGTCAGAGTGAAAGGCGATGGCACTTTTTCCTATCAGGGGATCGCGATCAA
GCTGGACGATCTCAAGTGTACGTTGTCAGGGGCGCATCAGATAAAGAATGCCGCACTGGCACTAGCAGCGGCCGAGGTCG
TTACCACAAAAGGGATTCGTGTCAATCGGCAGGCCATGCTCAGTGGTGTGGCCTGCGCAAGCTGGGATGGTCGGCTTGAA
ATACTCCAGAATACGCCCACCGTGCTTGTCGACGGCGCCCATAATCCGGCGGCTGCCAATGTCCTCTGTCAGGCGCTTCG
AAATCGGAATGCGGATGACCATCGGCTCCTTATATTCGGAGCGCTCGGCGACAAGAACTATCGCCTTATGCTAAAAAAAT
TACTGCCGTTTTTCGATCATGTCATTTTCACAAAACCAAAGACGGAGCGTGCGGTCGATGTGGCCGATCTGATCAGGGCG
GCAAGACACTATGACCGCTCTGCCGAAGCGATAGAAGACAGCTGTGAAGCGTTGCAGCGGGCATTATGCCTTACCGGTAA
AAATGATATGATCTGTATTACCGGTTCGCTTTATCTTGTCGGTGAAATAAAAAAAAGGTATGCAGAAAATCTTCATGCTG
CATGCAAGCACGGCATTTCTCCCGCTGTTGCAAGCTGA

Upstream 100 bases:

>100_bases
CCATGAATCAGAACAGGCTCCACATTTTGGAGGACGGTCAGACTGTCCGTATTTAGCAGGATGATGTCCTTCTCTTAAGA
TTTTTCGACCTCAGGCAGTC

Downstream 100 bases:

>100_bases
AATTGGATTGTTGCCAGAGGTGATGATGAACCGAAAAAATTCATTATACGAGGAAGTGGGGATAAAGTCCATCCTTCGCC
CTGAAGGACCGAGAAGCTGG

Product: folylpolyglutamate synthase / dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MDGIDYGISGSRPEGSAKNIVCEVIINCTMENVQKEDPQRLLDLNCNIIRLGLDPIRHLLERMENPQLKYPAVLIGGTNG
KGSTAAILSCILKESGYKVGLYTSPHLTSICERIRINGSLISEERMQDCFGMIQSSLEEPLTYFEVLTASAYLYFYQEKV
DIAVLEVGMGGRLDATNVVTPLVSVISNVHLEHQEYLGRHLRDIAAEKAGIIKENSVCVTAVTQKAVLDLLSETCRQKEA
TLYRLGKDIRVRVKGDGTFSYQGIAIKLDDLKCTLSGAHQIKNAALALAAAEVVTTKGIRVNRQAMLSGVACASWDGRLE
ILQNTPTVLVDGAHNPAAANVLCQALRNRNADDHRLLIFGALGDKNYRLMLKKLLPFFDHVIFTKPKTERAVDVADLIRA
ARHYDRSAEAIEDSCEALQRALCLTGKNDMICITGSLYLVGEIKKRYAENLHAACKHGISPAVAS

Sequences:

>Translated_465_residues
MDGIDYGISGSRPEGSAKNIVCEVIINCTMENVQKEDPQRLLDLNCNIIRLGLDPIRHLLERMENPQLKYPAVLIGGTNG
KGSTAAILSCILKESGYKVGLYTSPHLTSICERIRINGSLISEERMQDCFGMIQSSLEEPLTYFEVLTASAYLYFYQEKV
DIAVLEVGMGGRLDATNVVTPLVSVISNVHLEHQEYLGRHLRDIAAEKAGIIKENSVCVTAVTQKAVLDLLSETCRQKEA
TLYRLGKDIRVRVKGDGTFSYQGIAIKLDDLKCTLSGAHQIKNAALALAAAEVVTTKGIRVNRQAMLSGVACASWDGRLE
ILQNTPTVLVDGAHNPAAANVLCQALRNRNADDHRLLIFGALGDKNYRLMLKKLLPFFDHVIFTKPKTERAVDVADLIRA
ARHYDRSAEAIEDSCEALQRALCLTGKNDMICITGSLYLVGEIKKRYAENLHAACKHGISPAVAS
>Mature_465_residues
MDGIDYGISGSRPEGSAKNIVCEVIINCTMENVQKEDPQRLLDLNCNIIRLGLDPIRHLLERMENPQLKYPAVLIGGTNG
KGSTAAILSCILKESGYKVGLYTSPHLTSICERIRINGSLISEERMQDCFGMIQSSLEEPLTYFEVLTASAYLYFYQEKV
DIAVLEVGMGGRLDATNVVTPLVSVISNVHLEHQEYLGRHLRDIAAEKAGIIKENSVCVTAVTQKAVLDLLSETCRQKEA
TLYRLGKDIRVRVKGDGTFSYQGIAIKLDDLKCTLSGAHQIKNAALALAAAEVVTTKGIRVNRQAMLSGVACASWDGRLE
ILQNTPTVLVDGAHNPAAANVLCQALRNRNADDHRLLIFGALGDKNYRLMLKKLLPFFDHVIFTKPKTERAVDVADLIRA
ARHYDRSAEAIEDSCEALQRALCLTGKNDMICITGSLYLVGEIKKRYAENLHAACKHGISPAVAS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=357, Percent_Identity=33.3333333333333, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI66932990, Length=357, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1788654, Length=404, Percent_Identity=29.9504950495049, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17553148, Length=422, Percent_Identity=26.7772511848341, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=422, Percent_Identity=26.7772511848341, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=422, Percent_Identity=26.7772511848341, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=432, Percent_Identity=29.8611111111111, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=433, Percent_Identity=28.4064665127021, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322718, Length=168, Percent_Identity=29.7619047619048, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24641571, Length=329, Percent_Identity=32.8267477203647, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=36.3128491620112, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 51026; Mature: 51026

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGIDYGISGSRPEGSAKNIVCEVIINCTMENVQKEDPQRLLDLNCNIIRLGLDPIRHLL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECHHHHHHHH
ERMENPQLKYPAVLIGGTNGKGSTAAILSCILKESGYKVGLYTSPHLTSICERIRINGSL
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCC
ISEERMQDCFGMIQSSLEEPLTYFEVLTASAYLYFYQEKVDIAVLEVGMGGRLDATNVVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCCHHHHHH
PLVSVISNVHLEHQEYLGRHLRDIAAEKAGIIKENSVCVTAVTQKAVLDLLSETCRQKEA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHH
TLYRLGKDIRVRVKGDGTFSYQGIAIKLDDLKCTLSGAHQIKNAALALAAAEVVTTKGIR
HHHHCCCCEEEEEECCCEEEEEEEEEEEECEEEEECHHHHHHHHHHHHHHHHHHHHCCCE
VNRQAMLSGVACASWDGRLEILQNTPTVLVDGAHNPAAANVLCQALRNRNADDHRLLIFG
ECHHHHHCCCEEECCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEE
ALGDKNYRLMLKKLLPFFDHVIFTKPKTERAVDVADLIRAARHYDRSAEAIEDSCEALQR
ECCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALCLTGKNDMICITGSLYLVGEIKKRYAENLHAACKHGISPAVAS
HHHHCCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDGIDYGISGSRPEGSAKNIVCEVIINCTMENVQKEDPQRLLDLNCNIIRLGLDPIRHLL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECHHHHHHHH
ERMENPQLKYPAVLIGGTNGKGSTAAILSCILKESGYKVGLYTSPHLTSICERIRINGSL
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCC
ISEERMQDCFGMIQSSLEEPLTYFEVLTASAYLYFYQEKVDIAVLEVGMGGRLDATNVVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCCHHHHHH
PLVSVISNVHLEHQEYLGRHLRDIAAEKAGIIKENSVCVTAVTQKAVLDLLSETCRQKEA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHH
TLYRLGKDIRVRVKGDGTFSYQGIAIKLDDLKCTLSGAHQIKNAALALAAAEVVTTKGIR
HHHHCCCCEEEEEECCCEEEEEEEEEEEECEEEEECHHHHHHHHHHHHHHHHHHHHCCCE
VNRQAMLSGVACASWDGRLEILQNTPTVLVDGAHNPAAANVLCQALRNRNADDHRLLIFG
ECHHHHHCCCEEECCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEE
ALGDKNYRLMLKKLLPFFDHVIFTKPKTERAVDVADLIRAARHYDRSAEAIEDSCEALQR
ECCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALCLTGKNDMICITGSLYLVGEIKKRYAENLHAACKHGISPAVAS
HHHHCCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]