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The map label for this gene is lcfB [H]

Identifier: 85375824

GI number: 85375824

Start: 3021266

End: 3022876

Strand: Direct

Name: lcfB [H]

Synonym: ELI_14985

Alternate gene names: 85375824

Gene position: 3021266-3022876 (Clockwise)

Preceding gene: 85375823

Following gene: 85375825

Centisome position: 98.98

GC content: 58.22

Gene sequence:

>1611_bases
GTGATCGACTGGAACCTTGGCGACATCCTTGATGCAATCGAACCTGCGGTCCCGGAAGATGCTCCGGCGCTGATTCACGG
CGACCGGATCATTACCTGGCCCGAAATGTCGAAGCGTTCGAACAATCTGGCGCGCAATCTGCGCGCGCAAGGTGCAGGCG
ACGGGGCGAAGATAGCCTTCTACATGCGCAACCGTCCCGAATACGGGGAATTGATGGCCGCGTGCTTCAAGGGCCGTCTC
ACGCACGTGAACATCAATTATCGTTACCGTCCCGAAGAGGTTTTCTACATCTTCGACGATTCCGATAGCGAGGTAATCGT
TTACAGTTCCGAGTTCCGCGACTGCATTCTTGAACTGAAGGAACGGTTGACCAAGGTTCATACATTTGTAGAAATCGGCG
ATGCTTCGCAGATCGCTTCCTTCGCGCTTCCTTACGAAAAACTTGTAGAAGGTAACGGCGCGCCTCTCGATATCGAGCGT
TCTCCCGATGACCTGCTGTTCATATATACAGGCGGTACCACCGGCATGCCCAAGGGCGTGATGTGGCGACACGATGATAT
GCGCAAGGCTCAGCTCGACGCGCAAAAGCTGCTGGGTCCGGTTCCCCAGACCATGGAGGAAATGGTGGCCCTGGTGAAGC
AGGGCGAGCTTGCGCGCATCACTTTGCCGGCGTGCCCGCTGATGCATGGAACCGGGTTCATTACTGCTATCGGCACGCTC
ATGTCGGGCGGGGCCCTCGTCACGCTGTCCGATCCATCCTTCGACGCGGAAGAATTGTGGGATGCGGTGGACCAGCACAA
GGTGCAGAGCATCGCCATTGTCGGTGACGCTTTTGCCAAACCCATGCTGCAGGCGCTGGACGCCCACCCGAAGCGCTGGG
ACACGAGCAGCCTGATTTCGATCATTTCGTCCGGCGTCATGTGGAGTAAACAGGTAAAGGCGGGTTTGTGCAAGCATATC
CCTCAGGTCATTCTCATGGACAGTTTCGGCGCTTCGGAAGGGTTGGGCTTCGGCTTGTCCGTGACGACCGCACAGGGCGG
CACCAATACCGCCAAATTCGGGATCGGGGAATTTTGCGACGTATTCGACGAGAACGACCGGAAGGTTGAGGCGGGGAGCG
GCGTGCCCGGTTTCATTGCCCGCAAGGGCGCAATCCCAACGGGGTACTACAAAGACCCGGAAAAGTCGGCCAAAACCTTC
AGGACGATCGACGGCGTGCGTTACTCCATCCCGGGCGACTGGTGCCAGGTGGAATCGGATGGCAGCCTCACCCTGCTGGG
CCGCGGTAGCGTGTGCATCAACACCGCAGGTGAAAAGGTTTATCCTGAAGAAGTGGAGGAGGTGCTCAAGACACATCCCG
CCATCGCCGACGCGCTTGTCGTCGGGGTGCCTGACGAAAAATGGGGCGAAGCGGTGACCGCTGTGGTGCATTTGAACCAG
GATGCCAAGTTCGAGGAGCAGGCGATCAAGGATCACGTGCGCGAACAATTGGCAGGCTACAAGACGCCCAAGGCGATTTG
CACCACGCAAACCCCGTTGCGCGCGTCCAATGGCAAGGCGGATTACGCGGCTGCCAAGCAAATCGCAAAAGCTGGTGTCG
GGGTGTCGTGA

Upstream 100 bases:

>100_bases
AGCAGCGGCCTTGCAGGGGTGGACGCAGGTCGCATGAGACGATGAATTAGGCGCACGCTACCGACAAAATCGTGGCCACC
AAGGAGAGAGGAATTAATAC

Downstream 100 bases:

>100_bases
AGGAGCACTCCACTTCGGACCACGATGCCGTCATCGTCGGGGCCGGGTTCAGCGGAATATACCTGCTTTACAAGCTGCGT
CAGGCGGGGTTCGATGTTGT

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MIDWNLGDILDAIEPAVPEDAPALIHGDRIITWPEMSKRSNNLARNLRAQGAGDGAKIAFYMRNRPEYGELMAACFKGRL
THVNINYRYRPEEVFYIFDDSDSEVIVYSSEFRDCILELKERLTKVHTFVEIGDASQIASFALPYEKLVEGNGAPLDIER
SPDDLLFIYTGGTTGMPKGVMWRHDDMRKAQLDAQKLLGPVPQTMEEMVALVKQGELARITLPACPLMHGTGFITAIGTL
MSGGALVTLSDPSFDAEELWDAVDQHKVQSIAIVGDAFAKPMLQALDAHPKRWDTSSLISIISSGVMWSKQVKAGLCKHI
PQVILMDSFGASEGLGFGLSVTTAQGGTNTAKFGIGEFCDVFDENDRKVEAGSGVPGFIARKGAIPTGYYKDPEKSAKTF
RTIDGVRYSIPGDWCQVESDGSLTLLGRGSVCINTAGEKVYPEEVEEVLKTHPAIADALVVGVPDEKWGEAVTAVVHLNQ
DAKFEEQAIKDHVREQLAGYKTPKAICTTQTPLRASNGKADYAAAKQIAKAGVGVS

Sequences:

>Translated_536_residues
MIDWNLGDILDAIEPAVPEDAPALIHGDRIITWPEMSKRSNNLARNLRAQGAGDGAKIAFYMRNRPEYGELMAACFKGRL
THVNINYRYRPEEVFYIFDDSDSEVIVYSSEFRDCILELKERLTKVHTFVEIGDASQIASFALPYEKLVEGNGAPLDIER
SPDDLLFIYTGGTTGMPKGVMWRHDDMRKAQLDAQKLLGPVPQTMEEMVALVKQGELARITLPACPLMHGTGFITAIGTL
MSGGALVTLSDPSFDAEELWDAVDQHKVQSIAIVGDAFAKPMLQALDAHPKRWDTSSLISIISSGVMWSKQVKAGLCKHI
PQVILMDSFGASEGLGFGLSVTTAQGGTNTAKFGIGEFCDVFDENDRKVEAGSGVPGFIARKGAIPTGYYKDPEKSAKTF
RTIDGVRYSIPGDWCQVESDGSLTLLGRGSVCINTAGEKVYPEEVEEVLKTHPAIADALVVGVPDEKWGEAVTAVVHLNQ
DAKFEEQAIKDHVREQLAGYKTPKAICTTQTPLRASNGKADYAAAKQIAKAGVGVS
>Mature_536_residues
MIDWNLGDILDAIEPAVPEDAPALIHGDRIITWPEMSKRSNNLARNLRAQGAGDGAKIAFYMRNRPEYGELMAACFKGRL
THVNINYRYRPEEVFYIFDDSDSEVIVYSSEFRDCILELKERLTKVHTFVEIGDASQIASFALPYEKLVEGNGAPLDIER
SPDDLLFIYTGGTTGMPKGVMWRHDDMRKAQLDAQKLLGPVPQTMEEMVALVKQGELARITLPACPLMHGTGFITAIGTL
MSGGALVTLSDPSFDAEELWDAVDQHKVQSIAIVGDAFAKPMLQALDAHPKRWDTSSLISIISSGVMWSKQVKAGLCKHI
PQVILMDSFGASEGLGFGLSVTTAQGGTNTAKFGIGEFCDVFDENDRKVEAGSGVPGFIARKGAIPTGYYKDPEKSAKTF
RTIDGVRYSIPGDWCQVESDGSLTLLGRGSVCINTAGEKVYPEEVEEVLKTHPAIADALVVGVPDEKWGEAVTAVVHLNQ
DAKFEEQAIKDHVREQLAGYKTPKAICTTQTPLRASNGKADYAAAKQIAKAGVGVS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=566, Percent_Identity=22.0848056537102, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI187761345, Length=556, Percent_Identity=25.7194244604317, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI187761343, Length=556, Percent_Identity=25.7194244604317, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI38505220, Length=502, Percent_Identity=22.1115537848606, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI42544132, Length=156, Percent_Identity=32.0512820512821, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI115511026, Length=488, Percent_Identity=22.1311475409836, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI58082049, Length=493, Percent_Identity=22.7180527383367, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI40807357, Length=330, Percent_Identity=24.5454545454545, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI157311624, Length=131, Percent_Identity=32.0610687022901, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI157311622, Length=131, Percent_Identity=32.0610687022901, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=532, Percent_Identity=25.5639097744361, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=24.7191011235955, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786810, Length=544, Percent_Identity=24.8161764705882, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=22.7184466019417, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=573, Percent_Identity=24.0837696335079, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=573, Percent_Identity=24.0837696335079, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=573, Percent_Identity=24.0837696335079, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=23.7226277372263, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17557194, Length=512, Percent_Identity=25.390625, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=481, Percent_Identity=25.7796257796258, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=541, Percent_Identity=22.550831792976, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=495, Percent_Identity=24.6464646464646, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=446, Percent_Identity=23.7668161434978, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=350, Percent_Identity=26, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=425, Percent_Identity=22.3529411764706, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=530, Percent_Identity=24.7169811320755, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=561, Percent_Identity=21.74688057041, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=544, Percent_Identity=22.9779411764706, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI18859661, Length=503, Percent_Identity=25.0497017892644, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355181, Length=477, Percent_Identity=24.1090146750524, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922652, Length=331, Percent_Identity=24.1691842900302, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 58317; Mature: 58317

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDWNLGDILDAIEPAVPEDAPALIHGDRIITWPEMSKRSNNLARNLRAQGAGDGAKIAF
CCCCCHHHHHHHHCCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEE
YMRNRPEYGELMAACFKGRLTHVNINYRYRPEEVFYIFDDSDSEVIVYSSEFRDCILELK
EECCCCCHHHHHHHHHCCCEEEEEEEEEECCCEEEEEEECCCCEEEEECCHHHHHHHHHH
ERLTKVHTFVEIGDASQIASFALPYEKLVEGNGAPLDIERSPDDLLFIYTGGTTGMPKGV
HHHHHHHHHHEECCHHHHHHHHCCHHHHHCCCCCEEEECCCCCCEEEEEECCCCCCCCCC
MWRHDDMRKAQLDAQKLLGPVPQTMEEMVALVKQGELARITLPACPLMHGTGFITAIGTL
EECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
MSGGALVTLSDPSFDAEELWDAVDQHKVQSIAIVGDAFAKPMLQALDAHPKRWDTSSLIS
HCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHH
IISSGVMWSKQVKAGLCKHIPQVILMDSFGASEGLGFGLSVTTAQGGTNTAKFGIGEFCD
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
VFDENDRKVEAGSGVPGFIARKGAIPTGYYKDPEKSAKTFRTIDGVRYSIPGDWCQVESD
HCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCC
GSLTLLGRGSVCINTAGEKVYPEEVEEVLKTHPAIADALVVGVPDEKWGEAVTAVVHLNQ
CCEEEEECCCEEEECCCCCCCHHHHHHHHHHCCCHHHEEEEECCCHHHHHHEEEEEEECC
DAKFEEQAIKDHVREQLAGYKTPKAICTTQTPLRASNGKADYAAAKQIAKAGVGVS
CCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIDWNLGDILDAIEPAVPEDAPALIHGDRIITWPEMSKRSNNLARNLRAQGAGDGAKIAF
CCCCCHHHHHHHHCCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEE
YMRNRPEYGELMAACFKGRLTHVNINYRYRPEEVFYIFDDSDSEVIVYSSEFRDCILELK
EECCCCCHHHHHHHHHCCCEEEEEEEEEECCCEEEEEEECCCCEEEEECCHHHHHHHHHH
ERLTKVHTFVEIGDASQIASFALPYEKLVEGNGAPLDIERSPDDLLFIYTGGTTGMPKGV
HHHHHHHHHHEECCHHHHHHHHCCHHHHHCCCCCEEEECCCCCCEEEEEECCCCCCCCCC
MWRHDDMRKAQLDAQKLLGPVPQTMEEMVALVKQGELARITLPACPLMHGTGFITAIGTL
EECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
MSGGALVTLSDPSFDAEELWDAVDQHKVQSIAIVGDAFAKPMLQALDAHPKRWDTSSLIS
HCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHH
IISSGVMWSKQVKAGLCKHIPQVILMDSFGASEGLGFGLSVTTAQGGTNTAKFGIGEFCD
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
VFDENDRKVEAGSGVPGFIARKGAIPTGYYKDPEKSAKTFRTIDGVRYSIPGDWCQVESD
HCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCC
GSLTLLGRGSVCINTAGEKVYPEEVEEVLKTHPAIADALVVGVPDEKWGEAVTAVVHLNQ
CCEEEEECCCEEEECCCCCCCHHHHHHHHHHCCCHHHEEEEECCCHHHHHHEEEEEEECC
DAKFEEQAIKDHVREQLAGYKTPKAICTTQTPLRASNGKADYAAAKQIAKAGVGVS
CCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]